LOCUS       c00360_gnlPro..         3658 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MHIJKGIP_360.
ACCESSION   c00360_gnlPro..
VERSION     c00360_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:44:30
            Original ID  :: gnl|Prokka|MHIJKGIP_360
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3658
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1779
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg360_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-42, bitscore:
                     132.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.4e-215, bitscore:
                     704.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RHNKILTGLPDTYGRGRIVGDYRRVALYGIDALIEGKQKDFAACD
                     RQGMRRYDFQLREEIADQIRALKGMKVMAESYGYDISKPAKDAREAFQWLYFGYLAAIK
                     TQNGAAMSVGRISTFLDIYIERDLQNGTLTEKEAQELVDHMVMKFRMVKFARIPSYNQL
                     FSGDPVWATLEVAGMGQDGRSMVTKNDYRFLHTLEDMGPAPEPNLTVLYSSRLPENFKK
                     YAANISVTTSSVQYENDDVMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYA
                     INGGVDEKTKQQVGPNYQPITSEYLDYDEVIAKYDKMMDWLAHLYVGTLNMIHYMHDKY
                     YYEAAEMALIDTKVERSFATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDF
                     PRYGNDDDRADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDG
                     RKAGEPLAPGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDER
                     SENLVHVLDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLT
                     REQQLDVIARTCHDRM"
     protocluster    1..3658
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2538]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2538
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3658
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3658
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1798..2538
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg360_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.1e-29, bitscore:
                     90.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             2969..3658
                     /locus_tag="ctg360_3"
                     /transl_table=11
                     /translation="MIINCMHGGWHKVKDLLSEIESYWTTRAEGYSEVNHKELNGMQKG
                     AWLEVLKGQFPEKAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAG
                     NLCERISFYKMDAQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFD
                     ANWYGYLYDEEKRLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDR
                     KFLKENGF"
ORIGIN
        1 cgtcacaaca agatccttac aggtcttccg gatacttacg gacgtggacg tatcgttggc
       61 gactacagac gtgtggctct gtacggaatt gacgctctga tcgaaggaaa acagaaagac
      121 ttcgcagcat gcgaccgtca gggtatgaga cgttatgact tccagcttcg tgaagagatc
      181 gctgatcaga ttcgtgctct gaaaggcatg aaagttatgg ctgaatctta tggatatgat
      241 atctccaaac cggccaaaga tgcaagagaa gctttccagt ggctgtactt cggatatctt
      301 gcagccatca aaactcagaa cggtgctgca atgagtgtcg gacgtatttc cacattcctt
      361 gatatttata ttgaaagaga tcttcagaac ggaactctta cagagaaaga agctcaggag
      421 cttgttgacc acatggtaat gaaattccgt atggttaaat ttgcacgtat tccttcttac
      481 aaccagttat tctccggtga cccggtatgg gcaactctgg aagtagcagg tatgggacag
      541 gacggacgtt ccatggtaac aaagaacgac tatcgtttcc tgcacacact ggaagatatg
      601 ggaccggctc cggaaccaaa ccttacagta ctttattctt caagactgcc tgagaacttc
      661 aagaaatatg ctgcaaatat ttctgttaca acaagttctg ttcagtatga gaatgatgat
      721 gttatgcgtc cggtatgggg cgatgactac agcatctgct gctgtgtatc tgctacagag
      781 acaggtaaag agatgcagtt cttcggtgct cgtgcaaacc ttgcaaaatg ccttctttac
      841 gcgatcaacg gcggtgttga tgagaagaca aaacagcagg taggaccgaa ctatcagccg
      901 atcacttctg aatatcttga ttatgatgaa gtaattgcaa aatatgataa aatgatggac
      961 tggctggctc atctgtatgt cggaacactg aacatgatcc actacatgca tgacaaatac
     1021 tactacgaag cagcagagat ggctctgatc gatacaaagg ttgagcgttc tttcgcaaca
     1081 ggtatcgcag gattctctca tgtagtagac tccctgtccg ctatcaaata tgcaaaagtt
     1141 aaagctatcc gtgatgaaga cggtattaca acagatttcc aggttgaggg tgacttccca
     1201 cgttatggta atgatgatga cagagcagac gagattgcaa caactcttct gtctacattc
     1261 cttgagaaac tgaaacacat ccatacttac cgtgattcca agccaaccac atctatcctt
     1321 actattactt ccaacgttgt ttatggtaag gctacaggat ctcttccaga tggaagaaaa
     1381 gcaggggaac ctcttgcacc tggcgcaaac ccatcttacg gtgcagagca gaacggtctt
     1441 cttgcttccc tgaactctgt tgccaagctt gactatgaag atgctcttga cggtatctcc
     1501 aatactcaga caatcaatcc tgatgctctg ggacacagtg atgacgagcg ttcagagaat
     1561 ctggtacatg tacttgacgg atactttgat cagggtgctc atcacctcaa tgtaaacgta
     1621 tttggtaaag agaaactgat cgacgctatg gaacatccgg aaaaagaaga gtatgcaaac
     1681 ttcacaatcc gtgtatccgg atatgctgtt aagttcatcg accttacaag agagcagcag
     1741 ttagacgtta ttgccagaac ctgccacgac agaatgtaat taatggatga tgatataatg
     1801 gataataaac agataaaagg ctatgttcat tctttagaaa gcttcgggtc cgttgacgga
     1861 cccggagtaa gatatgtgat ttttttaagc ggctgtgcca tgagatgtca gttctgtcat
     1921 aatcctgata cctggaaaat gaagcaggga gagctttata cggcagatga gcttcttaag
     1981 aaagctttac gttataaggg ctactggggc agcaaaggcg gtatcactgt aagcggtggt
     2041 gagccattgc ttcagatgga ttttcttaca gaatttttta agaaggcaaa ggcagagggt
     2101 gtgcatacga atcttgatac cagtggaaat cctttcactg atcaggaacc atggcattcc
     2161 gggtggctgg agcttatgaa gtatacagat cttgtaatgc ttgatatcaa gcagattgac
     2221 gagcaggaac atattaagct taccggtcat tccaataaga atattcttgc gatggcgcgg
     2281 gaactttctg atatgaaaaa acctgtatgg atccgtcatg tgcttgtgcc gggcggaagt
     2341 gataaggatg aatatctgca ccggttagct gattttatcc atactctttc caatgtggag
     2401 agagtggagg tgctccctta tcatacgctt ggtaagttta aatgggagaa tctggggctt
     2461 tcttatccac tggagggggt taatcctccg acgcaggaaa ggattgataa tgcccggaag
     2521 attcttggag ctatctgagt aaaaatatat agctgtcata tcctcttttt gggggtatgg
     2581 cagctttgtt ttttgtttag aaaggggacg gacgcgaagc aggagtatag tcattccagg
     2641 tgcggtttcg gtatctaaga gattctaagc cctcgaaaat ccgctaaaag catgagacga
     2701 aaagtccgga attactcgct atcgctcaga caagggactt ttcgtctcat aacgcagatt
     2761 tccgaggact aagaatctct aagatgcctg caaatgcacc tggaatgact atactcctgc
     2821 ttcgctggtt ttggttaaga tataatacaa ggtggagtgt gaattataag aaggactttg
     2881 tttatataaa aatgaattga gatgtgaaat atatcccccg ggagggcttg ttggggggag
     2941 ggtggatttg atataatttt ggtcatatat gattataaat tgcatgcatg gaggttggca
     3001 taaggtgaag gatttattat cggagattga gagttactgg actacccggg ctgaggggta
     3061 ttctgaagtt aatcataagg aattgaatgg aatgcagaag ggggcatggc ttgaggtgtt
     3121 gaaagggcag ttcccggaga aagcgaagga tgagattaag attttggata tcggaacagg
     3181 acctggattt tttcctgtta ttctggctga ggcgggatat aaggtgactg ctgttgatta
     3241 tacgcaggag atgttggata ccgcgaagag gaatgccggt aatctttgtg agcggatttc
     3301 tttttataag atggatgctc agaatctgga atttgaggat gatgtgtttg atgttgtgat
     3361 cagcaggaat ctgacatgga atctgaagga tcctaagaga gcttatgaag agtggtgtcg
     3421 tgtactgaaa ccgggcggaa agcttcttaa tttcgatgca aactggtacg gttatcttta
     3481 tgatgaggag aaacgcctgt cttatgagga agaccgaaag agtgtggaga gtgagcatct
     3541 ggatgatcat tacctctgta cggatattga cagaatggag aagattgcac tgcagatgcc
     3601 tctttcttct attaacagac cgtcctggga caggaagttt ctgaaggaga acgggttt
//
