LOCUS       c00363_gnlPro..         3625 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MHIJKGIP_363.
ACCESSION   c00363_gnlPro..
VERSION     c00363_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:44:30
            Original ID  :: gnl|Prokka|MHIJKGIP_363
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3625
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..456
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg363_1"
                     /sec_met_domain="Spermine_synth (E-value: 3.8e-24,
                     bitscore: 73.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HYENALRFIRRCEAKYDLIIVDSSDPFGPSEGLFTREFYGNCYNA
                     LKDDGIMVNQQGSPFYAEDAHAMQRSHKRIASTFQISRVYQAHIPTFAAGYWLFGFASK
                     KYHPVDDLDAEAWNALNLRTRYYTTRLHKGAFYLPAFLEDMLKEVED"
     protocluster    1..3625
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:3625]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..3625
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..3625
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3625
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             460..1317
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Arginase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg363_2"
                     /sec_met_domain="Arginase (E-value: 4.8e-77, bitscore:
                     247.2, seeds: 235, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMLPNIETFIGCESNFEEASIVLYGAPFDSTTSFRPGARFGPSAM
                     RHESFGLETYSPYQDKDLIDISVFDSGDLELCFGSSEMALSDIQKRAEEILKAGKFPLL
                     LGGEHLVTLGAVRAAAAKYPDLHIIHFDAHADLRDDYLGAKLSHACVLRRCHEIVGDGH
                     IHQFCIRSGEREEFQFASRHTDFHPFTFEGLGETVRELKEKQVPVYFTIDLDCMDPSVF
                     PGTGTPEAGGVSFLELLKAIRIVSQTNVVGADVNELAPMLDVSGVSTATACKVLRELLL
                     AIAK"
     CDS             1365..2624
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg363_3"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 4.5e-45, bitscore:
                     142.8, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 4.6e-34,
                     bitscore: 105.7, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRVLVIGCGGVASVAIQKCCQVDEVFTEMCIASRTKEKCDALVE
                     KLKGKTKTVLTTAKVDADDVDQLIELINSYKPDLVMNIALPYQDLTIMDACLACGVNYM
                     DTANYEPEDTDDPEWRAIYEKRCKEEGFSAYFDYSWQWAYRKKFEDAGLTALLGCGFDP
                     GVTQAYCAYALKHEFDQIDTIDILDCNGGDHGYAFATNFNPEINLREVSAPGSYWENGH
                     WVEIPPMDIKREYNFDQVGDKDMYLLHHEEIESLAKTIPGVKRIRFFMTFGQSYLDHMR
                     CLEDVGMLSTTPIQYNGQEIVPIQFLKALLPDPASLGPRTKGKTNIGCIFTGVKDGKEK
                     TYYIYNVCDHQECYHEVGSQAISYTTGVPAMCGALMMLTGKWIRPGVYTVEEFDPDPFL
                     DALDRYGLPRSESHDPKLVD"
     CDS             2624..3625
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg363_4"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 7.5e-10,
                     bitscore: 27.2, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGLDKRAPFTATGGIIPPEFQNIKTPCYILDEKALIKNAKLLGE
                     VSERTGCKMLLAQKAFSNYDCYRFFEPYLAGTEASGLFEARLGAEEMPGKEVHVFCAGY
                     RADEFEELLQYADHIVFNSPSQLLRFGAKAKVAGKSVGLRINPECSTQEGHEIYDPCAP
                     GSRMGTTRAQWDEAFQKNPELLDLLDGLHFHTLCEQDSDDLETTLVSVKKHFGDLASRV
                     KWINFGGGHHITRPGYDIERLERCIRMVQEEWKAEVYLEPGEAWALNAGFLLTSVLDVL
                     KNGDTQIAIVDASAACHMPDVLEMPYLPPLLGTDEKEEGGITVRLGGPTCLSG"
ORIGIN
        1 cattatgaaa atgcgctgag atttatccga agatgcgaag caaaatatga tctgatcatt
       61 gtggattcct ctgatccgtt tggtccgtct gaaggattgt ttacccgtga attttacgga
      121 aattgttaca atgcgctgaa ggatgacggg attatggtaa accagcaggg cagcccgttc
      181 tacgcagaag atgcacatgc catgcagcgt agtcataaaa ggattgccag caccttccag
      241 atcagccgtg tttaccaggc acatatccca acctttgctg caggttactg gctgttcgga
      301 tttgcaagta aaaaatatca tccggtggat gacctggatg cagaggcatg gaatgcatta
      361 aatctccgta cacgttacta tacgaccaga ctccataaag gggcatttta tcttccggct
      421 tttctggaag acatgctgaa agaagtggag gattaagtta tgatgttacc taatatagaa
      481 acctttatcg gctgcgaaag caattttgag gaagcatcca ttgttcttta tggtgcaccg
      541 tttgattcta caacaagttt tcgaccgggt gcccgctttg gcccatcagc catgcgccat
      601 gagagttttg gcctggaaac ctacagtccg tatcaggata aagaccttat agacatcagc
      661 gtatttgaca gcggagatct ggagctttgc tttggaagca gtgaaatggc actttcagac
      721 attcaaaaga gagcagaaga aattttgaaa gcaggaaaat tcccactgct tctgggagga
      781 gaacatctgg taacattggg agcagtgcgt gccgcagctg caaaatatcc ggatcttcat
      841 attattcatt ttgatgctca tgcagatctg agagatgatt atctcggagc aaaattaagt
      901 cacgcctgtg ttcttcgaag atgccatgaa attgtgggag acggccatat ccatcagttc
      961 tgtatccgaa gtggagagag agaagaattt cagtttgctt ccagacacac agattttcat
     1021 ccgtttactt ttgaaggact gggagaaaca gtcagagagc tgaaagagaa acaggttccg
     1081 gtatatttta caattgatct ggactgtatg gacccgtcag tatttccggg aacagggact
     1141 ccggaggcgg gcggcgtgag ctttttagag cttcttaaag caatccgcat tgtttctcag
     1201 acgaacgtgg taggagcaga tgtcaatgaa ctggctccta tgctggatgt gagtggtgtt
     1261 tcaactgcaa cagcctgcaa ggttcttcgt gaactgcttc tggcaattgc aaaataatat
     1321 taatagaaaa cagtaatata tcaacataaa aaaggagaaa aattatgagt agagtattag
     1381 tgatcggctg cggaggagta gccagtgtgg caattcagaa atgctgccag gttgatgagg
     1441 tattcacaga aatgtgtatt gcaagccgta caaaggaaaa atgtgatgca cttgtagaaa
     1501 aactgaaagg aaagacaaaa accgttctta ctacagctaa ggtggatgca gatgatgtgg
     1561 atcagctgat tgaactgatc aacagctaca aaccagatct ggtaatgaac atcgcacttc
     1621 cttatcagga cctgacgatc atggatgcat gtctggcatg tggtgtaaac tatatggata
     1681 cagcaaacta tgagccggaa gatactgacg atccggagtg gcgtgcaatt tatgaaaaac
     1741 gctgcaaaga agaaggattt tctgcatact ttgattattc ctggcagtgg gcatatcgta
     1801 aaaaattcga agatgcagga cttacagcac ttcttggatg tggatttgac ccgggtgtta
     1861 cacaggctta ctgtgcctat gcattaaagc atgaatttga tcagattgat accatcgata
     1921 tcctggactg caacggcggt gatcatggat atgcatttgc aacaaacttt aacccggaga
     1981 tcaatcttcg tgaagtaagt gcaccgggaa gttactggga aaacggacac tgggtagaaa
     2041 tcccgccgat ggatatcaag agagaatata actttgacca ggttggagac aaggatatgt
     2101 atctgctcca ccatgaagag attgagtctc ttgcaaaaac aattccgggt gtaaaacgta
     2161 ttcgtttctt catgactttt ggacagagct atttggatca catgcgttgt cttgaagatg
     2221 taggaatgct ttctacaaca ccgatccagt acaatggaca ggagattgtt ccaatccagt
     2281 ttttgaaagc actgcttccg gatcctgcaa gccttggacc acgtacaaaa ggaaaaacaa
     2341 atattggatg tatctttact ggtgtaaaag acggaaaaga aaaaacatac tatatttaca
     2401 atgtatgtga ccatcaggaa tgctatcacg aagtaggaag ccaggcgatc agctatacaa
     2461 caggtgttcc ggcaatgtgc ggagcactta tgatgctgac cggaaaatgg atcagaccag
     2521 gtgtgtatac tgtagaagaa tttgatccgg atccgttcct ggatgctctt gatcgttatg
     2581 gactgccgcg cagtgaaagc cacgatccaa agctggtaga ctgatgggag gattggataa
     2641 aagagctccg tttacagcca ccggcggaat tattccgccg gaattccaga atataaaaac
     2701 accctgctac atcctagatg aaaaagcact cataaaaaat gcaaaactgc tcggtgaagt
     2761 gtctgagcga acaggctgta agatgctcct ggcacagaaa gcgttcagca attatgactg
     2821 ctatcgcttt tttgagccgt atcttgcagg tacggaggcc agcggactgt ttgaggcacg
     2881 actcggagca gaggaaatgc cgggaaaaga ggttcatgta ttctgtgcag gctaccgggc
     2941 tgatgaattt gaagaacttc ttcaatatgc ggatcatatc gtgtttaatt caccatcgca
     3001 gctccttcgt tttggtgcaa aggcaaaagt agcaggaaag agcgtcggat tgcggatcaa
     3061 tccggaatgt tccactcagg aaggacatga gatttatgat ccctgtgctc cgggaagccg
     3121 tatgggtacg acaagagcac agtgggatga agcatttcag aaaaatccgg aacttttgga
     3181 tttactcgat ggccttcact tccatactct ctgcgagcag gattccgatg atctggagac
     3241 aactcttgtt tcagtaaaga agcattttgg tgatctcgct tccagagtaa agtggatcaa
     3301 ttttggcggt ggccaccata tcacaagacc aggttacgat attgaaagac tggaacgttg
     3361 tatccgtatg gtacaagagg aatggaaggc agaggtttac cttgaacccg gagaagcgtg
     3421 ggcattaaat gcaggctttc tcctgaccag tgtgctggat gttctgaaaa acggagatac
     3481 acagattgca attgtggatg ccagcgcagc ctgtcatatg ccggatgtac tggaaatgcc
     3541 ttatcttccg ccacttttgg gaacagatga gaaagaagag ggtggaatca ctgttcgcct
     3601 tggagggccg acctgcctgt cagga
//
