LOCUS       c00125_gnlPro..         7064 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OFFLCLLB_125.
ACCESSION   c00125_gnlPro..
VERSION     c00125_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:50:42
            Original ID  :: gnl|Prokka|OFFLCLLB_125
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7064
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7064
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[346:3326]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      347..3326
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7064
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7064
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             39..344
                     /locus_tag="ctg125_1"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLFVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             complement(347..1069)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.2;
                     E-value: 2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg125_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-36, bitscore:
                     114.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKS
                     GTPYRMEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(1086..3326)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg125_3"
                     /sec_met_domain="Gly_radical (E-value: 6.4e-47, bitscore:
                     146.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDRDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             complement(3754..6801)
                     /locus_tag="ctg125_4"
                     /transl_table=11
                     /translation="MNKKFSTLMAGFLLTSAFASAQISPNNLEPVTFENDKVTSGEYII
                     LRDLTNDGISNDDLALTATLSGNTLTTKTEVLSTAKLADAVWKFTVKEETNAAGTKSYT
                     YTLQNKTTGKYLTFNLNGNLTIDASKAGKAEVGTEYSSIFAAVGGTTAANVEFKNDGNG
                     ILYLKGVTVANALALAQTNNVVKVDETGTALILCKYTAEQNADIAKMNDTMGGEGFNLT
                     SAGDKFEGNIFKNGDEYLNLKAFEVATGTIANVPAGIYFVVVDENMPATLKGKDAQLDK
                     KEEFEAATFVAVDPLKNLNINEVPRGKGKGFWFTTVKGSDFHFNNAAGEVSKKDEIYVG
                     NACFTVINPTLLDETTYQIYTNNARVLKEEGKDDHIAAGKQFIGFVKDQDVDYMITNDE
                     DHALTFKATNETLYDVKGLLNDTDAPAIYTIQFVSGEDKEGVSEYGQWLTVDQHATQTG
                     LRLASVEEINEQDPRFQFVVKKISDYNTDTKKYKKVTFANRQSGVEIELSLFKEGDAYI
                     VYTTDNSNNWDVYPEYIDGNDVNGDVTFGNGVKWATTKIALNPVTVEDKFASFVNREEG
                     AGLVTFALAKNDEAAPEFYVGAKRNAKGLMTGEIFSYSDALDAAQFELEKSEKFLTALN
                     TYMYVKNGRPITSTEKDTVAFYTYKVKAFDADQDNMYLKWGSNDFDLGATGLNFVIKEN
                     IDGSVSLIDYNTTNKLDGNSLKVADKLKDGKTAPWATASAYDLTTLLSEGLKTYMVEES
                     PLVSLEAVPQHISFEAVRGGFMTMDENKDARLAIANEASEDLTFWVDTVHSNLNIPSFY
                     ITKGGNFLYNAVDSASYYAARKNYRFNLENKATGDAKLIFKAGELVSSDTLRTIVDGKS
                     VLVAEKDNAPKKIKGGLKNFQFQIVRAEEGSDEYVIRKDGQYVCQYNNYFYMKADKSEA
                     YRFVIEKQSAPTANEGIATSEVKVIAGEGNVTIAGAAGKKVVISNILGQVVANTVISSD
                     NATIAAPAGVVVVAVEGEAAVKAIVK"
ORIGIN
        1 attgttaact ttggatgtta acttaaaaat attcgaagat gagaagagct agtttgtata
       61 atacattttc agcaagtcta ttgttcgttt gcaacgcatg tctttcttca tgtgtctcac
      121 aaatacccct tacaaaggaa aaaccggtaa acaattccac ttatcaagtc gaatatctgt
      181 tcgaacacga agggtgtaag gtctatcgtt tcatggatat gggacattac atctatttca
      241 ccaactgcga gggagatgtt accagtattg aaagcgacag cgtccgcaca gtcaaccgga
      301 tcagccgtaa accgatcatc atcaaagagc agacactcga ttaggcttat acggttactc
      361 cgtgtttcat aaagatcgct ttcgcattgg ccagacgttc tgccgaaagc ggttccacac
      421 ctttcagctt atagtccagg ccttgtgcct catatttgta agctcccatt gtatgataag
      481 gcagaagttc ggctttctgg accacgttat atgaactgat atattcggcc agcttgctca
      541 aggcttcatc atcgtccgtc aggccgggca cgatcacgtg gcgaatccat acagggatgc
      601 cgcgtttctc cagttcatca agaaagcgta aagtattatc gagttttacg gccgtcaaac
      661 ggggatgaag accggcgtcg atcgtcttga tatctaacag aaccagatcg acctgttcga
      721 gcacctccaa tgctttaccg gaacagatat agccggaagt gtccaacgcc gtatgaatac
      781 cgttttcacg gcaaaggcgg aaaaactctt taaggaattc aggttgtaac aaaggctcac
      841 caccggtcac ggtaacacca cctttagcga taaaattctt atagcgcagc acttcggcaa
      901 gcaaggcttc cggttccatt cgataaggcg tcccgctttt tacctcccaa gtatccggat
      961 tgtggcaata cagacaacga agcggacatc cctgcatgaa aacaacaaag cgaattccgg
     1021 gaccgtcaac ggtcccgaaa ctctctaaag aatgtatacg ccccaacata ttaatcaatg
     1081 gacaattata aactgctgtt gatggtgcgc gagatgacgt cgagctgctg ttcgcgtgtc
     1141 agcttcacga agttgacggc atagcccgaa acgcggatcg taagctgcgg gtatttctcc
     1201 ggatgctcca ttgcatctaa caacaagtcc ctatcgaaca cgttcacatt cagatgctgg
     1261 ccaccgtcag gcgtaaaata gccatccaac aggctcacca aattatttat ctgaatatcg
     1321 cgctccttac ccaatgtagc cggtgaaacg gcaaatgtat aggagatacc gtcatgcgca
     1381 tgctggaaag gcagtttggc aacagaagcc aaagcggcaa cagccccttt cgtgtcgcgt
     1441 ccgttcatcg ggttggctcc cggagcgaac ggcgtaccac ccggacggcc gtcaggagtc
     1501 gttccggttt tctttccgta aaccacgttt gaagtgatcg tcaggataga ttgtgtcgga
     1561 gttgccttgc ggtatgtctg atgctggcgc agatattcca tgaacttctc ggtaatgtcg
     1621 acagcaatac tgtccgtgcg gtcgtcattg tttccgaaag gtacatattc gccttcgcgt
     1681 tcgaagtcga cagccaaacc acgttcgtcc cggatcactt tcactttcgt atcacggatg
     1741 gcagccagcg agtcggcaac gatagaaagg ccggcgatac cagttgcgcg gatacgttcc
     1801 acatctccgt catgaagccc catctcgaat gcttcatacg catatttgtc gtgcatgtag
     1861 tgaatgatct tcagagcatt gacataaact tttgccaacc agcgcatcat ctgctcgtat
     1921 ttctccatca cttcgttgta atccagatac tcgctggtga tcggttcgaa acggggagcc
     1981 acctgcacac ccgatttctc gtcacgtccg ccattgatcg catacaacaa gcactttgcc
     2041 agattggcac gagctccgaa atactgcatc tgcttaccga tcttcatcgg agacacacag
     2101 caggcgatac cgtaatcgtc gccatagtcc ggacgcatca gatcgtcgtt ctcgtactgg
     2161 atggcagagg tatcgatcga tacttttgcg cagaagtttt tccagttatc cggagcattg
     2221 ttgaaccaaa gcaccgtcag gttgggttcg ggtgccgggc cgaggttata caaggtatgc
     2281 agataacggt aagatgtctt tgtcaccagc gaacggccgt caacaccttg tccgcccagc
     2341 gattcagtca cccaaacagg gtcgccggag aacaggtcgt tgtattccgg agtacgcagg
     2401 aaacggacaa tacgcagttt gataataaac tggtcgatca attcctgtgc ctcttcttcc
     2461 gtcagcttgc cttcgcaaat atctttttcg atatagatat cgaggaaagt agaagtacgt
     2521 ccgagagaca tggctgctcc atcctgatcc tttacggctg ccaggtaagc cagatacaag
     2581 aactgcacgg cttcgcgggc atcctttgcc ggacgtgtca catcgaaacc ataaccggca
     2641 cacattttct caaatgcttt cagcgctttg atctgctccg ttatctcctc acggctctga
     2701 atgatttctt ctgtcagttc ctgtatatcg agacgtttct ggaaacgttt cttttcttcg
     2761 atcaggatct gggttccgta cagaggtaca cgacggtaat caccgatgat acgtccgcgt
     2821 ccgtaggcat caggcaaccc ggtaatgatg gcggaacgac gtgccgcaat catctcgtct
     2881 gtataggctg aaaaaacgcc ttcgttgtgc gtctttctgt atttggtgaa aatctctttt
     2941 gtcatcgggt ccagatgata gccataggct tccagactgt tttcgaccat gcggataccg
     3001 cctttcggga agataccacg tttcaacggg gcatctgtct gcaagccgac gattacttcg
     3061 ttttccttat cgatgtaacc gggtccgtat gtgtcaatcc cctggggaag cttcgtttcg
     3121 gcatcatata ctcctttctc cctttcaacc ttaaacatct cggtgagttt gttccacact
     3181 ttcttcgttt tttccgaaga gggtacgagg aacgtatcgt ctccgttata aggtttgtaa
     3241 ttgttctgga taaagtctct tacattgatc tcctttttcc aggtttctcc tttgaactgc
     3301 gcccagttct cgttttcaaa cttcataagc ttatcttgtc tatttcggtt taggcacgca
     3361 aagatacgtc ttttccccct atctgaaaag gatcgtaaag gacgagggat gttaaactgg
     3421 ggaaaatacc tacaaatagg gataagggaa ataaaaacga agccccggca gtacgaatac
     3481 cgccggggcc tgttttttgt ctgccgtccg gtcttcacag agggaaaagc agtagaactt
     3541 ttaatttcat tactttagta ttattaatat taaatccaac cgctcgttcg cctgtcgcag
     3601 ggtaattgaa accgtagatc aagaaaacct cttacatacg gtttcacgca ggttgtctag
     3661 aattatttga ttataaaatg tcattgtgcc tgtgtaaggg tggttcgcta accaccctta
     3721 cgatagacag tattgttttt ttagtatcaa tcgttactta acgatagctt ttacagcagc
     3781 ttcgccttca acagctacaa ctactacacc cgccggagca gcgatagttg cgttgtcaga
     3841 agagattact gtgttagcta ctacctggcc taagatgttg ctgataacaa ctttcttacc
     3901 ggcagcacct gcgattgtta cgttaccttc gccagcgatg actttcactt cagatgttgc
     3961 gatgccttcg ttggctgtcg gagcagactg tttttcgatg acgaagcgat aagcttcaga
     4021 cttgtcagcc ttcatgtaga agtagttgtt gtactggcat acatactggc catctttacg
     4081 gatcacatat tcgtcactac cttcttcggc acgtacgatc tggaactgga agttcttcag
     4141 accacccttg atcttcttcg gagcattatc tttttctgca accaaaacag acttgccgtc
     4201 aacgatagta cgcaaagtat cggaagaaac caattcacct gctttgaaga tcaacttggc
     4261 atcacctgtc gctttgtttt ccaagttaaa gcgatagttc ttgcgagcag cgtagtagct
     4321 tgcagagtct acggcattgt acaagaagtt tccacccttc gtgatataga atgacgggat
     4381 gttcaagttc gaatgaaccg tgtcaaccca gaatgtcaag tcttcgcttg cttcattggc
     4441 aatagccagg cgagcatctt tgttttcatc cattgtcatg aaacctccac gaacagcttc
     4501 gaaagatata tgctgcggaa cagcttccaa acttactaac ggagattctt ctaccatgta
     4561 agtcttcaga ccttcgctaa gcagagttgt caagtcgtaa gccgaagcag tagcccaagg
     4621 agcagtcttg ccatctttca atttatcggc aaccttcaag ctattaccgt cgagtttatt
     4681 agtagtgtta taatcaatca agctaacaga accatcaata ttttctttga taacgaaatt
     4741 caaaccagtt gcacctaaat cgaagtcgtt agaaccccat ttcaaataca tattgtcttg
     4801 atcagcatca aaggctttaa ccttatatgt atagaaagca accgtgtcct tttctgtaga
     4861 agtgatagga cggccgttct tcacatacat atatgtattc agagcagtca agaacttctc
     4921 gctcttttcc aattcgaact gagcagcgtc caaagcatca ctataagaga atatttcacc
     4981 ggtcatcaaa cctttagcat ttctcttggc acccacatag aattcaggag cagcttcatc
     5041 gttcttagcc aaggcgaatg tcaccagacc tgcaccttct tcacggttta cgaaagaagc
     5101 aaacttgtct tctacagtta ccggattcaa cgcaatcttg gttgtagccc atttaacacc
     5161 attaccgaat gtaacgtctc cgtttacatc attaccatca atatattcag gataaacatc
     5221 ccagttattg ctattatcag tcgtatatac aatgtaagca tcaccttctt tgaacaaaga
     5281 caattcaatt tcaacaccag actgacggtt tgcaaacgtt acctttttgt attttttggt
     5341 atccgtgttg taatcgctaa tctttttaac aacaaactgg aagcgcggat cctgttcatt
     5401 gatttcttct accgaagcga gacggagacc agtctgggtt gcatgttggt caactgtcaa
     5461 ccactggcca tactcgctta ccccttcttt gtcttcaccg ctgacgaact ggattgtgta
     5521 gatagccgga gcatccgtat cgttcaacag acccttaaca tcataaagtg tttcgtttgt
     5581 agccttgaat gtaagggcat gatcttcatc attcgtgatc atgtaatcta catcctggtc
     5641 ttttacaaaa ccgatgaact gtttaccggc agcaatatgg tcatccttac cttcttcttt
     5701 caaaacgcga gcgttgttag tataaatctg ataagttgtt tcgtccaaca aagtcgggtt
     5761 gatcactgtg aaacaagcgt tacccacata gatttcatcc ttcttagaaa cttctcctgc
     5821 cgcattgtta aaatggaagt cagagccctt taccgttgta aaccagaacc ctttaccttt
     5881 accacgcggt acttcgttga tgttcaggtt tttcaacgga tcaacagcta cgaatgtagc
     5941 agcttcgaat tcttctttct tgtccaattg ggcatctttg cctttcaatg tagccggcat
     6001 gttttcgtct acaacaacaa agtatatacc ggcagggaca ttagcaatag ttcctgttgc
     6061 aacttcgaac gccttcaaat tcagatattc atcaccgttt ttgaagatgt tgccttcaaa
     6121 cttgtcaccg gcagatgtca gattgaagcc ttcgccaccc attgtgtcgt tcatcttggc
     6181 gatgtcggca ttctgttctg cagtgtattt gcaaaggata agagcggtac cagtttcatc
     6241 taccttaacc acattgtttg tctgggccaa tgctaacgca tttgcaaccg tcaccccttt
     6301 cagatacaag attccgtttc catcgttttt gaactcaaca tttgcagctg tcgtaccgcc
     6361 tacagcggca aagatagagc tgtattctgt tccaacctca gctttgccag cttttgacgc
     6421 atcgatagtc aaatttccgt taagattgaa agtcagatac ttgccagtcg ttttgttttg
     6481 taaggtataa gtgtacgact ttgtaccagc agcatttgtt tcttccttta cagtgaattt
     6541 ccaaacagca tctgccaact tcgcagtaga aagcacttct gttttagtag tcaatgtgtt
     6601 accacttaaa gtagctgtca aagccaagtc atcattactt atcccatcat tcgtcaagtc
     6661 acgaaggata atgtactcgc ctgaagtcac tttgtcattt tcaaacgtta ccggctccaa
     6721 attattgggc gaaatttgcg cagaagcgaa cgcacttgtc agcaggaacc ctgccatcag
     6781 agtagaaaac ttcttgttca taatctaaaa tgtttaatta ataataatat gtatattgaa
     6841 tatcgttctt acttcctgtt taacaggttt ttaccaaatt cctgtatttt gacgagataa
     6901 aacgtacgtt ttttttagtc atttttgagg ggctttctgg agggtttgtg ttggaaagct
     6961 ggtttttcgt acttgttaag ctatctgatt gataagaaag aatatagaaa aggaaaaata
     7021 attggaagaa aggattgcag gtttaaaaag tagttgtatt tttg
//
