LOCUS       c00053_gnlPro..        15682 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PPMDHLBH_53.
ACCESSION   c00053_gnlPro..
VERSION     c00053_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:28:51
            Original ID  :: gnl|Prokka|PPMDHLBH_53
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15682
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..372
                     /locus_tag="ctg53_1"
                     /transl_table=11
                     /translation="FMPGKPQVWYLDLFAGKNDYEAVRRAGAGGHKEINRTNLSMEDIE
                     EALKKDVVMTQLKLLRFRKNFPAFEKMNNIKIQAVGSTICFTWENEGYIARSNADFRTC
                     EFKVEGIDPQGDTVFNLEN"
     protocluster    1..15682
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4074:9510]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4075..9510
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..15682
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15682
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             714..1970
                     /locus_tag="ctg53_2"
                     /transl_table=11
                     /translation="VKSTERLIFHIDVNSAFLSWESARRVSQGLPDLREIPSCVGGDPK
                     KRTGIVVAKSIPAKKYGIQTGEPVAMALRKCPNLMIVPSDFELYDKCSRAFKSICASFA
                     PVMESFSIDEVFLDMTGTSLIYPDPVAAAYELKDKIHRELGFTVNVGISTNKLLAKMAS
                     DFQKPDRVHTLFPDEIPEKMWPLPARNLLFLGKASEQKLLNQGIRTIGDLAKEREANVQ
                     LWLGEKPGHQLWQYARGIDHSTVKAVPDEAKGFSVETTFNDDITSTEQVLPILLEQCDV
                     LATRMRRKGKKCNCISVTFRTLDFRNRSHQTKLENATDLTDEIYTNATRLFTEFWKGQP
                     LRLIGVALTGLTDGSFEQMSLFEDTETKERRRKLDAAMDEIRMKFGNDKITRASIMNSH
                     ASIGRKARAQIKNEDRQEE"
     CDS             complement(2020..2730)
                     /locus_tag="ctg53_3"
                     /transl_table=11
                     /translation="MNYLESQNVSYNTGAALVPLVCQSCIYDLSYGNPKVRPTAKMGEE
                     ACLQAFFVTDARTGNIGAGTGATVGRLYGMKQSMKSGLGIAAVSAGDFQMAAVVIVNAL
                     GDIFNPQNGLKIAGLKTPDRSGFLDSVQELYRFMTPHDQFTGNTTIGAVITNGAFSKAE
                     LNKIASMTRCAYARCINSVATMADGDSIYAASIGDVPVDINMAGTLAAEVMAQAIQNAI
                     HTSQIPDEEFLKYV"
     CDS             complement(2730..2975)
                     /locus_tag="ctg53_4"
                     /transl_table=11
                     /translation="MSLLKEISITELTNLRIGHASDHDGQTGVTVLYFPQGAKVGCDIS
                     GGGPASLSPLLLLPTIPSTLLCYPADLPMDWLRLTV"
     CDS             complement(3305..3679)
                     /locus_tag="ctg53_5"
                     /transl_table=11
                     /translation="MNTTTTTTAAAKRSSTLYMVELAMMIAIILLMSFTPLGYLRTPGL
                     SITLLTIPVAVGAIILGPKGGAVCGFTFGATSFYMAVCHRKFCFRSRTFQYQPGSNFYC
                     MYRSTCTGRMDHRTYFCSIT"
     CDS             complement(4075..4584)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg53_6"
                     /sec_met_domain="ALS_ss_C (E-value: 6.1e-23, bitscore:
                     70.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKGMKKRWISLYVENQVGVLSKISGLFSGKSYNLDSLTVGTTED
                     PTVSRMTIATVSDDETFEQIKKQLNRMIEVIKVIDFTDVFVRMKEILYIKVFNCSKEDK
                     VELFQIAETFKAKVIDYGKDSLLLEFVQTATKNDAVVKLIKEEFHRIEVVRGGSVGIES
                     ISMMER"
     CDS             complement(4602..6320)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg53_7"
                     /sec_met_domain="TPP_enzyme_C (E-value: 6.5e-49, bitscore:
                     155.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQISGNKLLVKALKEEGVEYLFGYPGACTIDISDELYKQDDVKI
                     ILPRHEQALVHEADAYARTTGKVGVCLVTSGPGATNLVTGLATANYDSVPLVCFTGQVA
                     RHLIGNDAFQEVDIVGITRSITKYGVTVRRREDLGRIIKEAFYIARTGRPGPVLIDLPK
                     DVMAELGSAEYPKNVNIRGYKPNTDVHIGQLKRAIKLLNKAKRPLFLAGGGVVISRAHE
                     IFREVVEKTKVPVVTTVMGKGSIPTDHPLYIGNLGMHGAYAANMAVSNCDVLFSIGTRF
                     NDRITGKLHEFAPHAQIIHIDIDTASISRNIQVDIPIVADAKEAITKMNEYVQECSTDK
                     WLNLIKNWKEEHPLKMRPNDLLSPMDILKEINEQFENSIIVTDVGQHQMLVSQYAEITE
                     GKQMIMSGGLGTMGYGLPGGIGAKIGNPDRPVIVISGDGGVQMNIQELATAVLEELPVI
                     LCIFNNEYLGMVRQWQKLFYGKRYAMTNLRAGALSRRTEGMEYPQYTPDFIRLAESYRA
                     KGIRVTKKEEIAAAFEEAKKNTKAPTVIEFIIDPEEMVYPMIKPGGTLEDMIMDC"
     CDS             7185..7739
                     /locus_tag="ctg53_8"
                     /transl_table=11
                     /translation="MDYLSNNVAVNLKRIRKSKSMSLDQVSEQTGVSKSMLAQIERGTA
                     NPSLGVLGKITSGLRIEFQELIQPPRVDCCLVTPAELVPTKEIAGQYRVWTCLPYEDTR
                     LVEVYRIEVEPGGVYISGSHGEKTREYLAVMEGVLTLECHGEKYEIHRDQVFKFETDQE
                     HVYRNTGKEKACCTCFFLDYH"
     CDS             8293..9510
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: Beta_elim_lyase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg53_9"
                     /sec_met_domain="Beta_elim_lyase (E-value: 1.8e-08,
                     bitscore: 23.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_3 (E-value: 2e-110, bitscore:
                     359.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKDTGLTFQDIKDKVNKYMIETYERFDFLAETAEGMYIYDENK
                     TPYLDFYAGIAVNSAGNCNPKVVAAAKDQLDDIMHTFNYPYTIPQALLAEKVCETIGMD
                     KIFYQNSGTEANEAMIKMARKYGIEKYGPNRYHIVTAKMGFHGRTFGAMSATGQPGNGC
                     QVGFGPMTYGFSYAPYNDLQAFKDACTENTIAIMVEPVQGEGGVHPATMEFMQGLRKFC
                     DENDMLLLIDEVQTGWCRTGAVMSYMNYGIKPDIVSMAKALGGGMPIGAICATAEVAKA
                     FTAGSHGTTFGGHPVSCAAALAEVNELLDRDLAGNAKKIGDYFAAKLEKLPHVKEVRHQ
                     GLLVGVEFDDTIGGVDVKHGCLDRHLLITAIGAHIIRMIPPLIVTEEDCDKAVAIIEDT
                     IKSLEK"
     CDS             9745..10365
                     /locus_tag="ctg53_10"
                     /transl_table=11
                     /translation="MKHLVITIGCEYGAKGNAIGKKIAQDLGIKFYDRDLVDEIIKEVG
                     IPTDIMDRVEEGGTIAGKGAQGDVRGSFSKYADLTDRAIHVQKQIIRKLAQKEACVIIG
                     RSADYILKDTEGIQLVRAFIYAPNDVRIHNIMESHNLSEKDAKLLLDEKDKRYHKRHLA
                     LTGSNRGDRHNRDILINSDFLGIQGTAEYLEDLIAKKYGKKEA"
     CDS             10369..11889
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AA_permease_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg53_11"
                     /sec_met_domain="AA_permease_2 (E-value: 1.8e-46, bitscore:
                     148.6, seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQKTSEFDKVLGAWDILVIAFGAMIGWGWVVSSGNWIESGGVLG
                     AAIAFAIGGIMIFFVGLTYAELTAAMPQCGGEHVFSYRAMGSTGSFICTWAIILGYVSV
                     ACFEACAFPTIITYLWPGFLKGYLYTVAGFDVYASWLIVAIVIAFLIMIININGAKTAA
                     VLQTVLTCIIGGAGIILIVASVVTGSVDNLQGQMFVGNGTGEAMKSIIKVAVITPFFFI
                     GFDVIPQAAEEINVPLKKIGKMMILSVVLAVVFYALVILAVGYILNPAEIIESQQGTGL
                     VTADAMAKVFGTKIMAKVIIVGGMCGIITSWNSFLLGGSRAMYSMAESYMIPPFFAKLH
                     PKHKTPVNSLYLIGALTMLAPFAGRTMMVWICDAGNFGCCLAYCMVSVSFLILRKKEPD
                     MARPYKVGHYKFVGFMAVVMSGLMLLVYCIPGSGGSLVFQEWLMVGSWSLLGVVFYAIC
                     KRKYKEDFGKLIELISDEDAASLMPEADDEELDVVIDAAIDRVLSSMA"
     CDS             12175..13359
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     374.1; E-value: 2e-114)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg53_12"
                     /sec_met_domain="Fe-ADH (E-value: 4.5e-115, bitscore:
                     374.1, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPFEFFIPQNITVGAGTLAKLPECAKKLGGSHAMLISGPTLRKM
                     GVVDKAADYLKDAGMAVDIFTDVEANPSVTTVEKATEAYKDSGADFIVALGGGSPMDVA
                     KAVGVTAKYGGSITEYEGAHKVPGKIVPLIAIPTTAGTGSEVTAFSVITDHSRDYKLTV
                     FSYELLPAYAILDPELLTSAPASVAAACGIDAFIHAEEAYVSTAASPFSDAMAEKAMEL
                     IGGNIRRFVARRTDLEAAEAMLTGSLFAGIAFSFARLGNVHAMSHPVSAFFNVAHGVAN
                     AVLLPVIAEYNALADHGRYLKIYNYISPIPAYEDEFEPLMLVDAIRELNEDIGIPEDLT
                     TAIRQAKKGEEISDEEIESKIDAMADDAMKSGNIAVNPRSSRKQDIVKLYYKAL"
     CDS             13629..14285
                     /locus_tag="ctg53_13"
                     /transl_table=11
                     /translation="MAKKKIYAVRKGHKTGLFATWAECQKAVSGYSGAEFRGFTEKEEA
                     LAFLNMETTGNVSGDKAKEAAGIVEVPENMVIAYVDGSFEKSIGRYAFGCVILTPDGQE
                     VRKSGSGSDPAGVAIRNVAGEMLGSMTAVNWAIENKYPAVEIRYDYEGVEKWVTGVWRA
                     KTPLTSKYAAHMQEAGKKVKISFCKIAAHTGNHYNEEADQLAKSALLKMDEEVRI"
     CDS             complement(14475..15566)
                     /locus_tag="ctg53_14"
                     /transl_table=11
                     /translation="MEKFKAFLRRKDIEISIKRYGIDALGAMAQGLFCSLLIGTIINTL
                     GTQFHISFLTTAVATVNDTQYTVGSLASAMSGPAMAVAIGYALHCPPLVLFSLITVGFA
                     SNALGGAGGPLAVLFVAIFASEIGKAVSKETKIDILVTPLVTIGIGVALSAWWAPALGS
                     AAMKVGNIIMWATNLQPFLMGILVSVFVGIALTLPISSAAICAALGLTGLAGGAAVAGC
                     CAQMVGFAVMSFRENKWGGLVSQGIGTSMLQMGNIVKNPRIWIAPILTSAITGPLATCL
                     FKLQMNGTPVSSGMGTCGFVGQIGVYTGWMNDIAAGTKTAVTGWDWAGLLLISFILPAI
                     LCPLINMFIRKLGWVKDGDMTLS"
ORIGIN
        1 tttatgccag gaaaacctca ggtatggtat cttgatcttt ttgcaggaaa aaatgattat
       61 gaagctgtca gaagagctgg ggcaggtgga cataaagaaa ttaacagaac gaatctttct
      121 atggaagata tagaagaagc attaaaaaaa gatgtagtaa tgacacaatt gaaattgctc
      181 cggttccgca aaaattttcc ggcatttgaa aaaatgaata atattaagat tcaggctgtt
      241 ggaagtacta tttgctttac ttgggaaaat gaaggttaca ttgcaagatc gaatgcggat
      301 ttccgtacat gtgaatttaa ggtagaggga attgatccac agggagatac agtttttaat
      361 ttggagaact aatgaccgga atgtgaagac aaatgattgt ctcttttgag agatacaggc
      421 tctgattttt tgatcgtaca cttgcttccg ggattacttt ttcccggaag caggtgtttt
      481 taccggctgt acagtgggaa tgtatgtaca ttctgaacag gaacagagta caggctgtgc
      541 gcagttttgc agatttacgg taaagtaaca gcgttacata aaatcattcg gatggtttcg
      601 gcaattagtt gttctgccct tgcgtttatg ttaagataaa agcaagtagc gaagcggatt
      661 gcgaatatct gcttgactta taattaagcg gcaggagctg agggggcaga acagtgaaaa
      721 gtacggaacg attaattttt catatagatg taaacagcgc atttctttcc tgggaatcag
      781 ccagaagagt gagccaggga ctgccggatc tccgggaaat tccctcctgt gtaggaggag
      841 atccgaagaa acggacaggt atcgtggttg ccaaatctat tcctgcaaaa aaatacggga
      901 ttcagaccgg cgagcctgta gctatggctc ttcggaaatg tccaaatctg atgattgttc
      961 ccagtgattt tgaattatat gataaatgtt cccgggcatt taagtccatc tgtgcttcct
     1021 ttgcgcctgt aatggaatcc ttttccattg atgaggtatt tttggatatg acaggcactt
     1081 ctctgatcta tcctgaccct gtagctgcag cttatgagct gaaggataaa attcacagag
     1141 agctgggatt tacagtaaat gtgggaattt ctaccaataa gctactggca aagatggcat
     1201 cggattttca gaaaccggac agagtacaca ctttgttccc ggatgaaatt ccggagaaaa
     1261 tgtggcccct tcctgcccgt aacctgctgt ttctgggaaa agcttccgag cagaagcttc
     1321 tgaatcaggg aatccgtacc atcggtgatc tggcaaagga acgagaggct aatgtacagc
     1381 tctggctggg tgaaaaaccg gggcaccagc tctggcaata tgccagaggt atcgatcatt
     1441 cgactgtaaa ggcagtaccg gatgaggcga agggcttcag tgtagaaact acttttaatg
     1501 atgatattac ttccacagaa caggtgttgc ccattttgtt ggaacagtgt gatgttctgg
     1561 ccaccagaat gcgcagaaaa ggaaagaagt gcaactgtat ttctgttact ttccggacat
     1621 tagatttccg aaaccgttct catcagacaa agctggaaaa tgccacagac cttacagatg
     1681 agatttatac aaatgcaacc agacttttta cagaattctg gaaaggacag ccactgcgcc
     1741 tgatcggtgt ggcactgacc ggtctcacgg acggttcctt tgagcagatg tcactgtttg
     1801 aagatacaga aacaaaagaa cgccgcagaa agctggatgc agctatggat gaaatccgta
     1861 tgaaatttgg caatgacaag atcacccgcg ccagtattat gaacagccat gccagtatcg
     1921 gccggaaagc cagggcacag ataaaaaatg aagacagaca ggaagaataa taaaagcgca
     1981 gagaaaaaag aatgatctct gcgcttttat agtggattgc tatacatatt tcagaaattc
     2041 ttcatccgga atctgagagg tatggatggc attctggata gcctgtgcca tgacttcagc
     2101 ggcaagagtt cctgccatgt taatatccac agggacatca ccaatgcttg ctgcatagat
     2161 ggaatctccg tctgccatgg tggcaacaga attgatgcat ctggcatagg cacagcgggt
     2221 catagaggca attttgttca gttcagcttt tgaaaatgcc ccattggtga tcactgcccc
     2281 tatggtggtg ttgccggtaa actgatcgtg aggtgtcata aaacggtata attcctggac
     2341 actgtccaga aatccgcttc tgtcaggtgt tttcagtcct gcaattttca ggccgttttg
     2401 tggattaaag atatctccca gtgcattgac aatgaccact gcagccatct ggaaatcacc
     2461 ggcagataca gcggcaatcc caagacctga cttcatggat tgtttcatac cgtagagcct
     2521 tcccacagtt gcaccggtac ctgcaccgat atttccggtt ctggcatctg ttacgaaaaa
     2581 tgcctgaagg caggcttcct cgcccatttt tgcagttggg cgtactttag ggtttccgta
     2641 ggaaaggtca tagatacagg actgacatac aaggggaact agtgccgcac cggtgttata
     2701 gctcacattc tgtgattcca gatagttcat cacaccgtca gccgcagcca gtccataggc
     2761 agatccgccg gataacacaa tagcgttgat gggattgtcg gcagtaacag gagaggcgag
     2821 agggaggcag gaccgcctcc gctgatatcg catcccacct ttgcaccctg tggaaagtaa
     2881 agaacagtta caccggtctg cccgtcatga tcagaagcat ggccgatgcg caaatttgta
     2941 agctctgtga tagaaatttc ttttaaaagt gacataaaaa tctccttctg ataaaaaatc
     3001 cctgactaca gtgacaacgt aatcagggac tttttattaa atattatgca cgtttcagtg
     3061 ctacagaaag tgccttggaa acaatacttc ccactacaaa gcagaaacct gcctcaatca
     3121 gtccctgaac tcctacaaag gctgtcacaa agacaatcgg attacttaca ccaagggtgg
     3181 aaacaatacc ttgaaataat cagtgttgta gaagaagaga cataaagagc ccatgaagaa
     3241 gaaagtattc agtaacggac atgccagtga agcaatgtaa taggaaaatt tcttcgccgg
     3301 tgatttatgt aatgctgcaa aaataagtcc tgtgatccat ccttccagta cacgtgctac
     3361 gatacataca ataaaagttg ctgccgggct gatattgaaa agtgcggctg cgaaagcaga
     3421 acttcctgtg acagacagcc atatagaagc tggttgcacc gaaagtaaat ccacatacag
     3481 ctccgccctt cggtccaaga ataatggcac ctacagctac cgggatggtg agaagagtga
     3541 tggataagcc gggagtacga aggtagccca gcggggtaaa ggacatgagc agaataatgg
     3601 caatcatcat agccagctct accatgtaaa gagtgctgct tctttttgct gcagccgtag
     3661 tagttgttgt tgtgttcata tgaattctcc ttttggtctg ataaaacggc gcaatgcgtc
     3721 ccctgctttt cagacagttt agagtatgag gaatcaggcg ttttgaaaaa tatgcatcag
     3781 aacaaaagat atgaaacaag aaatatcttc cggtaaaaat ttccggtgtg tagacgccgc
     3841 cttgcaggtc gcctcacgaa tttaaccaga ctgtaaagtg gatgacttta tccaccagac
     3901 tgtcagatgt ggaaaacaga tgcattttct ttcgaataac gctcttgtaa acaataacac
     3961 atttgattaa aactgcaatg ttttatagta tgtttgaaaa taaatacagg atatagcgtt
     4021 gttcgttgag aagtatctac acactatatc ctgtaaaaaa tattgattat gagattatcg
     4081 ttccatcata ctaatagact caattcctac gcttcctcca cgtaccactt caattctgtg
     4141 aaattcttcc ttgatcagtt tcactactgc gtcattcttg gttgctgtct ggacaaattc
     4201 caggagtaga ctatctttgc catagtcaat gacctttgcc ttgaaggttt cggcaatctg
     4261 aaacagttca accttatctt ctttggaaca gttaaaaacc ttaatataca gaatctcttt
     4321 catgcggaca aatacgtctg tgaaatcaat aaccttaata acctcgatca tacggttcaa
     4381 ctgttttttg atctgttcaa aagtttcatc atcactgaca gttgcgatcg tcattcgaga
     4441 tactgtgggg tcctcggtgg ttcctacagt aagactgtcc agattgtagc tttttccgga
     4501 gaaaagtccg gagattttgg acagaacacc tacctggttc tcaacataga gagaaatcca
     4561 tctttttttc attcctttat ccataataat tttcctcctg atcaacagtc cataatcata
     4621 tcttccagag ttccgcctgg tttgatcata ggataaacca tttcctcagg gtcaatgatg
     4681 aattcaatta ctgtaggtgc ctttgtgttc ttttttgctt cctcgaaggc tgctgcaatt
     4741 tcctctttct ttgtgacacg gattcctttt gccctgtagc tctctgcaag tctgatgaaa
     4801 tccggtgtgt actgtggata ttccatgcct tcagtgcggc gggaaagtgc tcctgcgcgc
     4861 agattggtca tagcatagcg tttgccgtag aaaagcttct gccactggcg taccattcca
     4921 aggtattcat tgttaaagat gcacaggata acaggaagtt cctccagaac tgcagttgca
     4981 agctcctgaa tgttcatctg cacaccgccg tcaccggaga taacgatgac aggtctgtcg
     5041 ggattcccga ttttggctcc gatgccgcct ggaagaccat agcccattgt tcccagacca
     5101 ccggacatga tcatctgttt gccctctgtg atttccgcat actgggagac aagcatctga
     5161 tgctgtccta catctgttac gataatactg ttctcaaact gttcgttgat ctctttcagg
     5221 atatccatcg gacttaaaag atcatttgga cgcattttaa gcggatgctc ttctttccag
     5281 tttttgatca ggttaagcca cttgtccgtt gaacattcct ggacgtattc gttcattttg
     5341 gtgatagcct cttttgcatc agcaacaatg gggatatcca cctgaatgtt tcgggaaatg
     5401 gatgctgtat caatatcaat gtggataatc tgggcatgtg gtgcaaattc atgaagtttt
     5461 ccggtgattc tgtcattgaa tctggttccg atggagaaca gcacatcgca gttgctgact
     5521 gccatgtttg ctgcataggc accatgcatg ccgaggtttc cgatataaag cggatggtca
     5581 gtaggaatgg aacctttgcc cattacggtt gtgacaaccg gtacttttgt tttttctact
     5641 acttcccgga agatttcgtg ggcgcgggag attactacac cgccgcctgc aaggaataaa
     5701 ggacgttttg ccttgtttaa aagtttgatg gcacgtttga gctggccgat atggacatct
     5761 gtgttgggct tgtatccgcg gatatttaca tttttaggat attcagcgct gccgagttct
     5821 gccattacgt cctttggaag gtcgatgagg acaggtcccg gacggccggt gcgggcaatg
     5881 tagaaagctt ctttgatgat acgtcccagg tcttcacgtc tgcggacagt aacaccgtat
     5941 ttggtgatgc ttctggtgat tcctacgatg tctacttcct gaaatgcatc gttgccgatg
     6001 aggtggcggg ctacctggcc ggtgaagcat acaagaggta cgctgtcata gttggcggtt
     6061 gcaagtcctg ttacaaggtt ggttgctccg ggaccgctgg ttaccagaca tacgccgact
     6121 ttgccggtgg ttctggcgta ggcgtcggct tcgtggacaa gggcctgttc gtgacggggg
     6181 aggatgattt tgacatcgtc ctgtttgtag agttcatcac tgatgtcgat ggtacaggct
     6241 ccggggtagc cgaagaggta ttctacgcct tcttctttta aggctttgac taagagtttg
     6301 ttgccggata tttgtttcat atttgggtaa acctcctttg ggtatgtgga tggggacgct
     6361 cggaaattta tttgttttcg ggtgcggttt cggagtctag gaactctaag tgtctgaaaa
     6421 tctgctaaaa ccgttcagaa aaatctcaaa ctcgccgcag atgcggctca gacagtgaga
     6481 tttttctgaa caacgcagat ttccaggcac taagagttac tacgactcct gcaaatgcac
     6541 ccgaaaacaa ataaatttcc gagctgtgtg ttggccaata cagggtgggg cggcgggttt
     6601 gatgtgacag attaaagcaa gcacgaacga ctttttgacc ctgatatata aatatagtaa
     6661 aaaacgccct gggcttgttt gcccagggcg aaaatgtttc cgcggtacca cctgtattca
     6721 gagtctggct ctgcactttg tcagtactga tatactgtgt tcctgtaacg tggaactgac
     6781 gttgaagcct acttggcatg tccgttcagg tcttgtggat cagacgggat atacggtttg
     6841 gttcactgct cgggggctac ttccacaatt cgttcacgaa agctcgcacc gaccgctttc
     6901 tctctgtgct tcccggggtt gtgtactcct ccctgtcatg gcatttggct gatataggga
     6961 tattcattgt ccgaataggg tggacaaatg ttttcttggg ttacattata catttataga
     7021 gaggaaattg tcaaccgggt tttggataat tgctgtaaaa tttatgttcc tgaatgaatt
     7081 ttggctacgt tactgagaac ggagtgaaca gtaatgtaat tgacagattt ctatattgct
     7141 gatataaaaa ataaataata cagctgtttg gaaggagaga acatatggat tatttatcga
     7201 ataatgtggc ggtgaatctg aagagaatcc ggaaatccaa gagtatgagt cttgatcagg
     7261 tgtctgagca gacgggggtg agtaagagta tgctggctca gattgagaga gggactgcca
     7321 atccttcttt gggtgtgttg gggaagatta ccagtggtct gaggatagag tttcaggagc
     7381 tgatacagcc gccgagagtg gactgttgtc tggtgacacc ggcagaactg gttcctacca
     7441 aggagatagc aggacagtac agggtatgga cctgtctgcc ttatgaggat acccgtctgg
     7501 tggaggtgta ccggatcgag gtggaaccgg gtggtgttta tatcagtggc agccatgggg
     7561 agaagacaag agaatatctg gctgttatgg agggtgtgct tactctggaa tgtcatggtg
     7621 agaagtatga aatccacaga gatcaggtgt ttaagtttga aacggatcag gagcatgtct
     7681 atcggaacac agggaaggag aaagcctgct gtacctgttt ctttctggat tatcattgat
     7741 acagttgtat cagggattct gttgttgaag caacccgccg taggcggaga atcctgtttg
     7801 caggattctc ccttgtgtga ttgctggaat tatcatagtc tggaactgag tagtgaagca
     7861 ggtgatttta tccgaagtat gagtgtaaaa tgtacaatat aatgcacaaa ataatgaaaa
     7921 agatacacca ggaaatgaat atctgaaaag aaagaatgta tgtaatggtt tgtcagtctg
     7981 ccatttcatg aagaggctgt gttgttatga attgttgaga aacctttata atatcgcatg
     8041 aatatgcagg aaatattgga tatacaataa ggagaacaca gagctttcgt tactgttcat
     8101 tccgttctca gtaacgatct ttctgtaaaa aatgtaaatg ttcaaaaaaa catgttgaca
     8161 aatcataagg gcagtgctat tgtaaggatg tacaaaataa tacacagaaa aagttaagca
     8221 aaggcgctta cccatacggg tcagtttgtc tttgcttttt ttattatctt aaggatgcag
     8281 gagggcacag ctatgaaatt aaaagacaca ggattaactt ttcaggacat taaggacaaa
     8341 gtaaacaagt acatgatcga gacttatgag agatttgatt tccttgcaga gacagcagag
     8401 ggcatgtaca tttatgatga gaataaaacc ccatatcttg atttttatgc tggtattgca
     8461 gtaaacagcg caggaaactg taaccctaag gttgtagctg ctgcaaagga tcagttagat
     8521 gatattatgc atacattcaa ctatccatac acaatcccgc aggcgctgct tgctgagaag
     8581 gtctgtgaaa caatcggaat ggacaagatt ttctatcaga actctggtac agaggctaac
     8641 gaggcaatga tcaaaatggc ccgtaaatat ggtattgaaa agtatggtcc aaaccgttat
     8701 catatcgtaa ctgcaaagat gggattccat ggaagaacct ttggtgccat gtctgcaaca
     8761 ggtcagccgg gtaatggatg ccaggttgga ttcggaccta tgacttatgg tttctcctat
     8821 gcaccgtaca atgaccttca ggctttcaag gatgcatgta cagagaacac aattgccatt
     8881 atggttgagc ctgtacaggg cgagggtggt gtacatcctg caacaatgga attcatgcag
     8941 ggacttcgta aattctgtga tgagaatgat atgcttcttc tgatcgatga ggtacagaca
     9001 ggatggtgca gaacaggtgc tgttatgtcc tatatgaatt atggtatcaa accggatatc
     9061 gtatctatgg caaaggctct gggcggtgga atgccaatcg gtgccatctg tgcaacagca
     9121 gaggttgcca aagctttcac agcaggttcc catggaacta cattcggcgg acatccggta
     9181 agctgtgcag ctgcactggc tgaggtgaat gagcttctgg acagagatct ggctggaaat
     9241 gcaaagaaga ttggcgatta ctttgcagct aagcttgaga agcttcctca tgtaaaagaa
     9301 gtaagacatc agggacttct ggtaggtgtg gaatttgatg atactatcgg tggagtggat
     9361 gtaaaacatg gttgtcttga cagacatctt ctgatcacag ctatcggagc gcatatcatc
     9421 cgtatgatcc caccgcttat tgttacagaa gaagattgcg acaaagccgt agcaatcatc
     9481 gaagatacaa ttaaatctct ggaaaaataa aaatccggca acggagtgat acagtaacag
     9541 gacaagggcg tcacgaatta tattcgtgcg cccttttatg atatgatatc agggtcaaaa
     9601 aatcaaaaag tcgttcgtgc ctgcacgata agatttttga acttgggacc tgccaaatat
     9661 gagaaagtag cgatttacat agcaaatcag cggatttcga atgtttgaga atctgataaa
     9721 aatttgagtg tgagagggaa gagcatgaaa catctggtaa ttacgattgg ctgcgaatat
     9781 ggagccaagg gcaatgccat cggtaagaag attgcacagg atctggggat taagttttat
     9841 gatcgtgatc ttgtggatga gattatcaaa gaggtaggca tccctacaga cattatggac
     9901 agagtagagg aaggcggaac catcgcaggt aaaggtgcac agggtgatgt gagaggttcc
     9961 ttttccaaat atgcggatct gacagaccgc gccattcatg tacagaagca gattatccgg
    10021 aagctggctc agaaagaagc ctgcgtgatc ataggacggt ctgcagatta tattctgaaa
    10081 gatacagaag gaatccagct ggtgagagca tttatttatg cacctaatga tgttcgcatt
    10141 cataacatta tggaaagcca taatctttcg gaaaaggatg caaagcttct gctggatgag
    10201 aaggataaac gttatcataa gagacatctg gcactgacag gaagtaaccg cggtgacaga
    10261 cataacaggg atattcttat taacagtgat tttctgggaa ttcagggaac agcagaatat
    10321 ctggaagatc tgatcgcaaa aaaatacgga aaaaaggagg cctgacctat ggaacagaaa
    10381 acatcagaat ttgacaaagt acttggtgca tgggacatac ttgtaattgc atttggcgcc
    10441 atgatcggct ggggctgggt agtttcttcc ggtaactgga ttgaatccgg tggtgtgctt
    10501 ggtgcagcta ttgcatttgc aatcggtgga atcatgatct tctttgtagg tctgacttac
    10561 gcagagctta cagcagccat gcctcagtgt ggtggtgagc acgtattcag ctacagggca
    10621 atgggttcta caggttcttt tatctgtacc tgggcgatta ttcttggata tgtaagtgtt
    10681 gcctgctttg aggcatgtgc atttcccaca atcatcactt atctgtggcc ggggttcctg
    10741 aaaggatatc tctatacagt tgcaggcttt gatgtttatg catcctggct gatcgtggcg
    10801 attgtgattg cttttcttat tatgataatc aacattaatg gagcaaaaac tgcagcagtt
    10861 ttgcagactg ttcttacctg tattatcggc ggtgccggaa ttattctgat cgtggcttct
    10921 gtggttacag gaagtgtgga taaccttcag ggacagatgt ttgttggaaa cggtactggt
    10981 gaagcaatga aaagtattat taaggttgca gttatcactc cgtttttctt tattggtttt
    11041 gatgttatcc cacaggcagc agaggagatt aatgttccgc tgaaaaaaat cggtaaaatg
    11101 atgatcctct ctgttgtgct ggctgttgta ttttacgcac tggttattct tgcagtaggt
    11161 tatatcctga atccggcaga gattattgag tctcagcagg gaacaggact tgtaacagca
    11221 gatgccatgg ccaaggtttt cgggacaaag attatggcca aggttatcat tgtaggtgga
    11281 atgtgtggta ttattacatc atggaactcc ttcctccttg gcggttcccg tgccatgtac
    11341 tctatggcag aatcctacat gattcctccg ttttttgcaa agcttcatcc gaagcacaaa
    11401 acgccggtta attctctgta tctgatcggt gccctgacta tgctggctcc ttttgcagga
    11461 agaacaatga tggtctggat ctgtgatgca ggtaacttcg gatgctgcct tgcatactgt
    11521 atggtctctg tctctttcct gatccttcgt aaaaaggaac cggatatggc aagaccttac
    11581 aaagtaggcc actacaaatt tgtaggtttt atggcagtag tcatgtcagg gctgatgctt
    11641 ctggtttact gtattccggg ttccggtgga agtctggtat ttcaggaatg gcttatggta
    11701 ggtagctgga gtttacttgg agttgtattt tatgcgatct gtaaacgtaa atataaagaa
    11761 gattttggta aacttattga actgatttct gatgaggatg cagcaagtct tatgccggag
    11821 gctgatgatg aagagcttga tgtggtaatc gatgcggcta ttgacagggt gctttcctct
    11881 atggcataac gctataaatg acatgacaat tattgctagt acatcgtttt cgaaataact
    11941 ataccactca cttgtctgcg aatccgattg cacaggtcta aaagcctcag cgtagcccgc
    12001 tacgcctgcg tttttatacc agcacactcg aatccgcatc ctacgcgatt ggtatagtta
    12061 tttacgaaac gctgtactag gaattatttt gggtaacaga gttttgaatt tgtgcagggt
    12121 agatgccctg cacattgcaa tattgaaaaa atacaggaaa agaggcagaa attaatgaat
    12181 ccatttgaat tttttattcc tcagaatatt acagtcggtg caggtactct ggcgaagctt
    12241 ccggaatgtg ccaaaaagct tggaggttct catgcaatgc tgatctcagg accaactctc
    12301 cggaaaatgg gagttgtgga taaggcggcg gattatctga aagacgccgg aatggcagtg
    12361 gatattttta cagatgtaga agccaatccg tctgtcacca cagtggaaaa agcgacagaa
    12421 gcgtataagg attccggtgc agattttatc gttgcgctgg gcggtggttc tcctatggat
    12481 gtcgcaaaag cagtgggagt tacggctaaa tatggcggca gtattacaga atatgagggg
    12541 gcgcataagg ttcccggaaa aattgttccg ctgatcgcaa tcccgactac tgcgggaacc
    12601 ggctcagagg ttacggcatt ttctgttatc actgaccaca gcagagatta taagctgacg
    12661 gtattcagtt atgaacttct tcctgcatat gcgatcctgg atcctgaact tctcacatct
    12721 gctccggcat ctgtggcagc agcctgcgga atcgatgcat ttattcatgc ggaggaggct
    12781 tatgtttcca cagcagcatc tcctttttct gacgcaatgg cagagaaagc aatggagttg
    12841 atcggtggaa atatccgccg ttttgtggca agacgtacag atctggaagc tgcagaggca
    12901 atgcttacgg gatctctttt tgcaggaatc gccttttcct ttgcaagact tggaaatgtc
    12961 catgccatga gccatccggt aagtgctttc tttaatgtag cacatggagt ggcaaatgca
    13021 gttcttcttc ccgtgatcgc agaatataat gctctggcag atcatggcag atatctgaag
    13081 atttataatt atatcagccc gatccctgca tatgaggatg aattcgaacc actgatgctg
    13141 gtggatgcca tcagggaatt aaacgaggat atcggaatcc cggaagacct gaccacagcc
    13201 atccgccagg caaagaaggg agaagagatt tctgatgagg agattgaaag caagattgat
    13261 gccatggcag atgacgccat gaagagcgga aatattgcag taaatccaag atcctccagg
    13321 aaacaggata ttgtgaaact gtattataaa gctttataag ataaagaata atgcagtatt
    13381 ttatgtgatt gctacaggta ggatattgat taaaaatatc tgttattata aaaaacgtgc
    13441 agaaacatcg aagtcgacag ataacttcag gcttgaaggt ttatctcgat ttcaaaatgt
    13501 atctgcacgt tgtttttttg agaaatttcc attgtataac ttgcctgata gaaagaaaga
    13561 ttatatagta ttaatcaaaa tatagtaaag taatgtgagt caaaatattg attgttcagg
    13621 aggattttat ggcaaagaag aaaatatatg cagtacgcaa gggacataaa acaggacttt
    13681 ttgctacatg ggcggagtgc cagaaggcag taagcggata ttcaggagct gagtttcggg
    13741 gatttacaga gaaagaggaa gcattggctt ttctgaacat ggagacaaca ggaaatgtat
    13801 ccggtgataa agcaaaagag gcggcaggaa tcgtggaagt acctgaaaat atggttattg
    13861 cctatgtgga cggaagtttt gagaaaagca ttggcagata tgcttttggc tgtgtgatcc
    13921 ttacaccgga cggacaggaa gtccgcaaat ccggaagcgg cagtgaccct gcaggagtcg
    13981 cgatccgaaa tgtggcagga gagatgcttg gttccatgac tgcagtaaac tgggccatag
    14041 aaaacaaata tccagctgtg gaaatccgct atgattacga aggtgtagag aagtgggtaa
    14101 caggtgtgtg gagagccaaa acacctctga ccagcaaata tgctgctcat atgcaggaag
    14161 caggaaagaa agtaaagatt tctttttgta aaatagcagc tcatacgggc aatcactaca
    14221 atgaagaggc agatcagctg gctaagagtg cattgcttaa aatggatgaa gaagtacgaa
    14281 tataaaaatc ttatcactga aaggcgattt ccaaagagat gaaaaattta tgtggatgac
    14341 aatttgagtg atccgcaggg aaattaatat acggaagtgg aaaaataaaa agcagctgtg
    14401 ttaccattga aactgttagc tgaaattttt atcaagtaac agtttcaatt gacaggtaac
    14461 gcagctgttt ttgcttatga aagtgtcata tccccgtctt tcacccatcc aagcttacgg
    14521 ataaacatgt tgatgagcgg acacaggatt gcaggcagga taaaggaaat cagcaaaagt
    14581 ccggcccagt cccagcctgt gacagctgtt tttgttcctg cggcaatatc attcatccat
    14641 ccggtgtaga caccaatctg tcctacgaaa ccacaggtac ccataccgga agaaaccgga
    14701 gtaccgttca tctgaagttt gaacagacag gttgcaagag gtccggtgat ggcagaagta
    14761 aggatcggtg caatccagat gcgtggattt tttacgatat ttcccatctg gagcatggat
    14821 gttccgattc cctgagaaac aagaccaccc catttatttt cgcggaagga cattactgcg
    14881 aaacctacca tttgtgcaca gcagcctgct acagcagctc ctccggcaag tccggtaagg
    14941 ccaagcgcag cacagattgc ggcagatgag ataggaagtg tcagtgcaat acccacaaat
    15001 acagaaacaa ggattcccat caggaacggc tgcaggttgg ttgcccacat aataatattt
    15061 cccactttca ttgcggcact tccaagtgcc ggagcccacc atgcggacag ggcaacacct
    15121 ataccgatcg tgacaagtgg agtgaccaga atatcaattt tggtttcctt agatactgct
    15181 tttccgattt cggaagcgaa gatggctaca aataatacag ccagcggtcc gcctgcaccg
    15241 cctaatgcgt tagatgcaaa gccaacagtg atcagtgaaa acaacaccaa cggcggacaa
    15301 tgtagcgcat agccgatggc aacagccatg gcaggaccgc tcatggcaga tgccagagag
    15361 cctacggtat actgtgtgtc gtttacagtt gccacagcag tggtcagaaa tgaaatatga
    15421 aactgtgttc caagtgtgtt gatgatcgtg ccgatcagca aggagcagaa aagaccctgc
    15481 gccatggcac caagggcatc gattccataa cgttttatgg agatttcgat gtctttgcgt
    15541 ctgagaaatg ctttaaactt ttccatagat tactttctcc tgttttttac tatgtataga
    15601 agggtgtata tacacctata tacgtttttc tcattatagt cacattcacg gagtaaatca
    15661 agtagttttg taactgttca ca
//
