LOCUS       c00245_gnlPro..         3437 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FGBGCNEI_245.
ACCESSION   c00245_gnlPro..
VERSION     c00245_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:09:10
            Original ID  :: gnl|Prokka|FGBGCNEI_245
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3437
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3437
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[85:3111]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      86..3111
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3437
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3437
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(86..814)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.8;
                     E-value: 3.2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg245_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-29, bitscore:
                     89.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFNTVYLN"
     CDS             complement(883..3111)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg245_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
ORIGIN
        1 gttttagatg cgaaattaga taattttatt gaaatgatga aatatttcca ttgatttata
       61 gcaacatctt gtcttcagta tatttctaat ttaaatatac ggtattgaaa tattcaccaa
      121 ataacgtttt ggctgtttcc aattgtttta gagtattttc ttttattccg tttagtttgt
      181 attcttttcc catggcttca tatttgtgta cgcctaatgt atgataaggt agtatttcta
      241 ctctttgaat catttgataa ttcttgaagt gctctcctaa tgcccgtata tcttcctcaa
      301 aggaactata gcctggaact aatacatagc gcaaccaaaa aggctttccg tttttttcaa
      361 gccatccggc ggtacgtatg gtttgttcgt tgctgcgttc tgtcagttgc cgatggcgca
      421 cattattaaa ttccttgatg tctaataata cgaggtctgt ccattgtagc agttccttta
      481 cttcttcatt ccatatactg ccgttggtat ctatacagat gtggatgctt tgttctttta
      541 atcgctggaa taacggaatc agtgcttttg cttgcaatgt aggctcgccg ccgctgaaag
      601 tcacacctcc ttttttaccg aaaaaagctt tttggctgac ggccatacgc acaatttcgt
      661 ctattgttgt ttccgtactt tcacctttgg tgtcaattgt gtcggggttg gcacaataga
      721 ggcatcggaa attgcatcct tgtaaaaata cgactaagcg taaaccggga ccgtcaaacg
      781 tccccattga ctcgtaagaa tggactctga tcataatggt ttgtctttaa actggtaagg
      841 gcaggtttaa aacccgcccc tacgatgaat gtatatgtga tgttacattc tttcgtggaa
      901 agaacggctg attacttcca gctgatgttc acggctcaat ttgatgaagt ttacggcata
      961 acctgatacg cggatggtta gttgcgggta tttttcagga tgttccattg catcttccaa
     1021 catttcacgg ttcagcacat ttacattcag atgatgtgct cctttggtga aataaccgtc
     1081 catcatggtt accaggttct ctatacgggt ttcctggtct acccctagtg attttggtac
     1141 gatagagaac gtgttactga tgccgtcctg ggcatcacga tagcgtagtt tggctacaga
     1201 actcaatgag gcgatggcac cgttcttgtc acgtccgtgc atcgggttgg cacccggagc
     1261 gaaagccact cctttggcac gaccatcagg agtggcacct gttttcttgc cgtacattac
     1321 attggaggtg atagtcagca gtgacagggt agggcgggca tttttgtata ccggatgttt
     1381 ctttaattct tcactgaaga aataaaccaa atctacccct aagtggtcta cgcggtcatc
     1441 atcattaccg aagcaaggat atgcattttc aatatcaaag ccttcggtca aacctatatc
     1501 attacgtttg gcacgtactt tgccgtattt gatggcagac aatgagtcaa tggcaatgga
     1561 cagaccggct acgccatatg ccagattgat acgtggatcg gtatcaatga atgccatttg
     1621 agccttttca taataatatt tatcgtgcat atagtgaatg atattcatcg catcattata
     1681 cacacgtgca atttgcatta atactttctt ataattagac catacttctt caaagttcag
     1741 caagtcccct gtcaatacag gaatatcttt caccattaca gttcctgtat tctcacaacg
     1801 tccgccattg atagccagca atagggcttt agccagattg caacgtgctc cgaagaactg
     1861 gatttgtttg ccgatagcct gataagaaac gcagcaggcg ataccataat catcacagtt
     1921 acgcacttcg cgcatcagcg tgtcgttttc gtattgtata gaagaagtgt cgatagaaac
     1981 ttgtgcacag aattctttga aaccttccgg caattccgga ctccacagta cagtcatatt
     2041 aggttcgggt gacggaccca gattgtatag tgtctgcaag aaacggaagg aagtttttgt
     2101 aaccttggta cgtccgtcgt tgaagcgtcc gccgatagac tcggttaccc aggtgggatc
     2161 acctgcaaag atatcgttgt atgattgcat acgcagatga cggaccatac gcaacttgat
     2221 aatgaactgg tctaccagtt cctgcgcaaa tgactcgtca attttaccat gatccaaatc
     2281 atattgaata tagatatcca ggaatgaaga aacgttgccc aatgacatag ctgctccatc
     2341 ttgttctttc acggcagcaa gatatgccat ataaacccac tgaacagctt cttgtgcatt
     2401 agtagcggga cgacccaagt cgagtccgta atattcaccc aatatcttta tttctttcag
     2461 tgctttgatt tgttcggcta cttcttcgcg caggcggata cgggcatcgg tcatcggact
     2521 ggtgatattg tgcaaatctt cttgttttgc ttcaatcagt cggtcgatac catacagtgc
     2581 caaacggcgg tagtcaccga tgatacgccc gcgtgcataa ttatcgggca gaccggtcag
     2641 gaaaccgagt gaacggaaag agcggatttc ttcggtgtaa gcatcaaata cgccatcatt
     2701 atgggttttg cgataatgag taaagatatc ctttactttt tcatctactt ccacaccgtt
     2761 ttcatggcag gctttttcta cgactttgat accaccataa ggtttgatgg cacggcgcag
     2821 aagttcatcc gtttgaagac ctacaatcag ttcgttttca cggtcaatgt atcctgcttt
     2881 atgtgaggtg atggtagata ctgttacatt atcaagggaa cgaactccgt tattggcacg
     2941 ttcttcttct agtgccttga ggcattcgtt ccacactttt ttagttcgct ctgtggggcc
     3001 tgccagaaat gaggcatcgc cctcataagg ggtaatgttg gtatgtacga agtctgaaac
     3061 attaatttcc ttactccaca atccatcctt aaaaactttg ttcatttcca tactgttatt
     3121 ccattattaa gagatttagg gctgcaaagg tactcctttt ttgtgagcta tgcatcacta
     3181 ttagtaggta taatgcgaat acttgtgcat ttttaagtat aaagtggtca ataaatgaaa
     3241 aaacaataag gactctttat aacaattcca taaaaaataa agctaattgg tggcaataaa
     3301 ccaattagct tctaaaagta gcggaaccgg gaatcgaacc cgagtttcag ccgtgagagg
     3361 gctgcgtcct aggccactag acgataccgc catcagttag tcttaattct ttttctaaga
     3421 cggtgcaaag ttagaag
//
