GCA_958414985.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaMKIECKOO_1 (original name was: gnl|Prokka|MKIECKOO_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	lanthipeptide-class-ii	1	12,669

gnlProkkaMKIECKOO_14 (original name was: gnl|Prokka|MKIECKOO_14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	NRPS	1,615	50,599	dipeptide aldehydes	NRP	94%

gnlProkkaMKIECKOO_18 (original name was: gnl|Prokka|MKIECKOO_18)

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	ranthipeptide	28,477	50,065

gnlProkkaMKIECKOO_62 (original name was: gnl|Prokka|MKIECKOO_62)

Region	Type	From	To	Most similar known cluster		Similarity
Region 62.1	cyclic-lactone-autoinducer	1	19,737

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	lanthipeptide-class-ii	1	12,669
Region 14.1	NRPS	1,615	50,599	dipeptide aldehydes	NRP	94%
Region 18.1	ranthipeptide	28,477	50,065
Region 62.1	cyclic-lactone-autoinducer	1	19,737

Compact view

gnlProkkaMKIECKOO_1 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 12,669 nt. (total: 12,669 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_1

allorf_007307_007405

Leader:

MGKAALNEQGCNSREVVLTA

Core:

ARLKPTFCNLTF

Core with dehydrations:

ARLKPDhbFCysNLDhbF

antiSMASH-DB 4.0 matches for allorf_007307_007405

19.2% NZ_KB907258.1: Lanthipeptides: allorf_010568_010702_lanthipeptide

2

RLKPTFCNL

10

R PTFC +

16

RFAPTFCAI

24

allorf_007545_007664

Leader:

MAHPLYGS

Core:

AQKGRTRFALYCVAVILRRFLCQGTYGAAAD

Core with dehydrations:

AQKGRDhbRFALYCysVAVILRRFLCysQGDhbYGAAAD

antiSMASH-DB 4.0 matches for allorf_007545_007664

22.6% NZ_KB912942.1: Lanthipeptides: AMYBE_RS44660_lanthipeptide

3

KGRTRFALYCVAVILRRFLCQ

23

G + F+L+C L LC

4

HGHSHFSLHCGDSTLSLLLCD

24

12.0% NZ_RYYV01000004.1: Lanthipeptides: allorf_108229_108468_lanthipeptide

1

AQKGRTRFALYCVAVILRR

19

+ + T + C+A +LRR

31

SSQAETTLSFCCIAELLRR

49

allorf_007561_007686

Leader:

MARRKKDVPGL

Core:

PYTASPLSCVAFFVKEHTGLLRIDTKRKRG

Core with dehydrations:

PYDhbADhaPLDhaCysVAFFVKEHDhbGLLRIDDhbKRKRG

antiSMASH-DB 4.0 matches for allorf_007561_007686

25.8% NZ_MSRJ01000005.1: Lanthipeptides: allorf_215951_216079_lanthipeptide

7

LSCVAFFVKEHTGL

20

L C A FVK GL

17

LVCQAVFVKMKLGL

30

16.7% NC_009727.1and 1 other:

NZ_LK937696.1 Lanthipeptides: allorf_1081979_1082068_lanthipeptide

Lanthipeptides: allorf_1214590_1214679_lanthipeptide

1

PYTASPLSC

9

P++ SP+SC

1

PHSRSPISC

9

allorf_008562_008687

Leader:

MEVAAMIYRAYRQSTAHFS

Core:

LSKKARPPPGTLLPSYMNCQII

Core with dehydrations:

LDhaKKARPPPGDhbLLPDhaYMNCysQII

MIBiG 3.1 matches for allorf_008562_008687

11.8% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A

8

PPGTLLPSYMNCQI

21

PG + M C +

8

TPGCKTGALMGCNM

21

antiSMASH-DB 4.0 matches for allorf_008562_008687

22.7% NZ_CP026729.1: Lanthipeptides: allorf_1517053_1517127_lanthipeptide

11

TLLPSYMNC

19

T+LPS+ +C

1

TILPSWQSC

9

15.4% NZ_SBIS02000011.1: Lanthipeptides: allorf_232888_233061_lanthipeptide

7

PPPGTLLPSYMNC

19

PP G+ PS+ C

1

PPEGSAHPSHYLC

13

14.3% NZ_CP050504.1: Lanthipeptides: allorf_0161193_0161369_lanthipeptide

8

PPGTLLPSYMNCQI

21

P GT P Y +C+

10

PCGTACPGYEHCRA

23

with 1 more sequence matches

11.8% NZ_JADMPO010000001.1: Lanthipeptides: allorf_303731_303931_lanthipeptide

5

ARPPPGTLLP

14

PPPG LP

15

CSPPPGCYLP

24

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_007307_007405 - Class II

Cleavage pHMM score: -6.50

RODEO score: 0

Monoisotopic mass: 1373.7 Da

Molecular weight: 1374.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1392.7 Da N = 1

allorf_007545_007664 - Class II

Cleavage pHMM score: -6.50

RODEO score: 0

Monoisotopic mass: 3381.8 Da

Molecular weight: 3383.9 Da

Number of bridges: 2

allorf_007561_007686 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 3299.8 Da

Molecular weight: 3301.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3319.9 Da N = 1; 3337.9 Da N = 2; 3355.9 Da N = 3; 3373.9 Da N = 4

allorf_008562_008687 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 2372.3 Da

Molecular weight: 2373.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2391.9 Da N = 1; 2409.9 Da N = 2

gnlProkkaMKIECKOO_14 - Region 1 - NRPS

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Location: 1,615 - 50,599 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 1614 - 50599:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg14_22

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaMKIECKOO_18 - Region 1 - ranthipeptide

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Location: 28,477 - 50,065 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaMKIECKOO_62 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 19,737 nt. (total: 19,737 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.