LOCUS       c00146_gnlPro..         3286 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MKIECKOO_146.
ACCESSION   c00146_gnlPro..
VERSION     c00146_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:30:10
            Original ID  :: gnl|Prokka|MKIECKOO_146
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3286
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3286
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[187:3131]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      188..3131
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3286
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3286
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             188..1459
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_1"
                     /sec_met_domain="HGD-D (E-value: 4.2e-57, bitscore: 181.4,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIETFGHYVENLTDKKPQSARRLLKTGWEAQNLKFRYMQDKRL
                     MPADRHLANLMMDTMLRPLQKPEASVIVSIFTPCEMMQEVGLHPYNVEGFSCYLSASKA
                     ERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLADF
                     YQVPVFAIDVPWNQTTENVQYVADQLKDLKIFLEKNTGKTISEDRLRERLACSKRTLEN
                     YKKYQQMRADRYVPSDLVTPLYAGMTNNILLGTAEEEKYTQMLLEDIKKAPAAKGKHIY
                     WMHTIPFWSDAVRQELCFSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNGSA
                     LRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRS
                     HGGEGQTSTRLGAFLEMLGGAENE"
     CDS             1452..3131
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_2"
                     /sec_met_domain="HGD-D (E-value: 1.1e-18, bitscore: 55.1,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 5.8e-66, bitscore:
                     209.7, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTKFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTDRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGEKKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLDPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADV"
ORIGIN
        1 attttggctt gtatgtctcg ggattttggc atattcatgc caaaacacct cgcagaatag
       61 tggtgtgtga acagtaacga aaattcaact tgacttccaa aaaagatatt attaaaatat
      121 tccgtggcac tatttgtgca ctgacaattg ataaattatt attaccataa tgaggaagag
      181 acagactatg gagattattg agacatttgg tcattatgtg gaaaatctga cggataaaaa
      241 gcctcagtca gcccgccggc tgttaaaaac cggctgggag gcgcagaatc tgaagttccg
      301 ttacatgcag gacaagagac tgatgcctgc agacaggcat cttgcaaatc tgatgatgga
      361 taccatgctg cggccccttc agaaaccgga agcttctgtt attgtaagta tttttacacc
      421 ctgcgaaatg atgcaggagg tgggattaca tccctataat gtagaaggat tttcctgcta
      481 tctttctgca agcaaagcag agagggcgtt tttgcagcag gcagagaatc agggaattgc
      541 agagacactg tgcagttatc acaagacatt tcttggtgca gcgcagaaag gacttctgcc
      601 aaagcctaaa tgtattgttt ataccaacct gacctgcgat gctaacctgc tcaccttccg
      661 taccctggca gatttttacc aggtacctgt atttgccatt gatgtaccat ggaatcagac
      721 cacagagaat gtacagtatg tggcagatca gttaaaggat ttaaaaatat ttctggaaaa
      781 aaatacagga aaaacaatct ctgaggacag attaagggaa cgtcttgcct gcagtaagcg
      841 gactctggag aattacaaaa aatatcagca gatgcgtgca gacagatatg tgccctctga
      901 tctggtcaca cccttgtatg caggaatgac aaacaatatc cttctgggaa cagcagaaga
      961 agagaagtat acacagatgc ttcttgaaga tattaagaaa gcaccggcag cgaaagggaa
     1021 acacatttac tggatgcata ccattccatt ctggtcggat gcagtgcgcc aggagctttg
     1081 cttcagtgag aaagcccaga tcgtaggatg tgaacttgca gaaacctgtg agccggattt
     1141 tgatcctgag aacccttttg aagcaatggc agaaagaatg gtatatcatt cactgaacgg
     1201 aagtgccctt agaaggattg aagccggtat ccgccatgcg aaacaggcag gagcagacgg
     1261 tgctgtgtgg tttggacatt ggggatgtaa acatacgctt ggtgcggcac agcttgcaaa
     1321 gaagaagttc gaggaagccg gtctgcccct tttgatcctg gacggtgacg ggtgtgatcg
     1381 aagccatgga ggagaaggac aaacttccac caggctggga gcttttctgg aaatgctggg
     1441 aggtgctgag aatgaataaa acttactatg tctgtaaata taccccaatt gaacttctgg
     1501 aatctcttgg cggggaatgt gagaacttta atcatatgcc ggagggattt gacctggccg
     1561 atcaggtttc ccatcccaat atctgcggtt ttggaaaaac acttctggaa ggagtcatgg
     1621 aggggaaaat caaggaactt gtcctggtga gctgctgtga tactatccgg agtgtctatg
     1681 acattctgct tgaaagcggc aagctggatt ttctctatat tttggatgtg cttcactgtg
     1741 acagtgcctg cagcagagag cgtatggctg tacagttaaa agggctggcg aaggcttatg
     1801 cacagtacaa aggcacaaag tttgatgccg gaaaattccg ggctgctttc catgcacagg
     1861 agaaaatcac aaagtcccat attgcagtgc ttggtgccag aatggggcag gaattatttg
     1921 aaatgaccag caaagctatg cctcttcctg tagagaatga tacttgcgtg cataaccgtt
     1981 ctgtgggaaa tatacttcca ccggagggtg cttcttttga tgagctgatg gactggtatg
     2041 caggtgagat tctggggcag attccatgta tgcgtatgat ggacccgaca gatcgtaaga
     2101 agctttataa tgatccgtct gttgcaggaa ttatttatca tacagtgaag ttttgtgatt
     2161 tttacagctt cgaatatgct gagatcaaga accatacaga tgtacctctt ttaaaaatcg
     2221 aatcagatta tactattcag agcagcggtc agcttcttac cagactggaa gcttttgcag
     2281 agagcattca gcctgaaaca ctggagcaga ccatagatgg agacacaaaa ggagagaaaa
     2341 aaatgggaaa aggttatttc gcagggattg acagtggttc caccagtaca gatgtggtga
     2401 ttttaaataa agatcatgag attgtgacca gtattatcct gcctacagga gcaggagctg
     2461 ctatcggagc agaccgtgcc cttgcagagg cattgaaaga ggccggattg cagagagaag
     2521 atattgatgc gctggttact accggatacg gacgtacagc tattaaaaat ggtgataaga
     2581 gtatcacaga gattacctgt catgcaagag gtgcacactt cctggatccg gaagtccgca
     2641 ctgtcattga cattggcgga caggacagca aggtgatccg actggatgag aacggtgcag
     2701 ttgcaaactt tgttatgaat gacaaatgtg cggcaggaac cggacgattt ctggaaatga
     2761 tggcacgtac catggaaatg aatctggatc agatgagcga atgtgggctg gaattcaaag
     2821 aagatattac aatttccagt atgtgtacag tatttgcaga atcagaagtt gtatccctga
     2881 tcgcccagaa taaggcaaca gatgatattg ttcatggcct gaataaggcg gttgccgtaa
     2941 aaaccgcagc tcttaccaga cgtgtgggcg gtgaggagaa atatatgatg accggtggtg
     3001 tttctaagaa taagggcctg gtaaagacgc tggaagagaa gcttggaaca aagctggtaa
     3061 tcagtgacaa ggctcagctt tgcggtgcac ttggcgcagc gctgtttgca gctgatatgg
     3121 ctgatgtata aaaattattt aaatatccag ggattttaca aggtggaaat ctctggaatt
     3181 tttgtacttt ttttataaaa gtttaatttt ctattgacga gttcaaattt aagagatata
     3241 gtataccacc aatgtcatta acttaagaga ttgttgcttg ttaaac
//
