LOCUS       c00269_gnlPro..         5697 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BLHNNCLI_269.
ACCESSION   c00269_gnlPro..
VERSION     c00269_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:37:11
            Original ID  :: gnl|Prokka|BLHNNCLI_269
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5697
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5697
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[132:3108]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      133..3108
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5697
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5697
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(133..867)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(868..3108)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.2e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(3279..5582)
                     /locus_tag="ctg269_3"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKEVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSTTKREDEWQTLLVTGIQLERDKERHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERK
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
ORIGIN
        1 taacaaaaca aacacatatt aaatagtcaa ggttagtcta ggcaaaaaat tattactatc
       61 gtttaaaagc taatcggatt agattgtttt ccctgattag ctgttgcata atcgcaaggc
      121 ttgatcgaga gatcaggcct tgttattttc tggtttgtag cgagagaata tcgctttcgc
      181 attatctaaa cgttcttttg ataagggttc cacccccttt aacggataat cgagacctaa
      241 ttgctcatat ttataggttc ccatcgtatg ataaggcaat aattccactt tgcgaaccac
      301 tttatatgaa ctgacatacc gagctaacgc ctccagccac tcgtcattat ccgtatatcc
      361 ggggacaata acatggcgaa tccaagtatc tatgccacgt ctctccaatt catccaagaa
      421 cagaagcgta ttatcctgct tgacacccgc caataacgga tgtaaatccg ggtttaaggt
      481 tttgatgtcc aataacacta agtccgcata atccaagact tcccaagctt ttgaggtaca
      541 tacgaaacca gatgtgtcca aagctgtgtg aagaccttgc tcttggcaca aacggaaaaa
      601 ctctttcaga aactcaggtt gcaataaagg ttccccaccg gtaacagtca cccccccacg
      661 ggcgataaaa ctcttatacc gtaagacttc cgtcaatagc tcgccgggag tcatctgata
      721 tttcaccttc cctttcgggt tccaagtatc cgggttatgg caatacaggc aacgcaacgg
      781 gcagccctgc ataaaaacga caaagcgaat tccgggacca tctacggtcc cgaaactctc
      841 taatgaatgt atcttacctt ttatcactta cagtgaatgg ttaatggttc ttgaaataac
      901 atccaactgt tgttcacgag tcagtttcac gaagtttacc gcatatccgg atacacggat
      961 cgtaagttgc ggatatttct caggatgttc catagcgtcg atcaacaaat ccttatcaaa
     1021 tacgttaaca ttcagatgct gacctccatc cggagtaaaa tagccatcca ataagctaac
     1081 cagattattg atctgcactt cacgctcctt tcccaacgta gccggagaaa cagcgaaagt
     1141 ataagagata ccgtcgtgcg catgctggaa tggaagcttc gctaccgaag ctagcgcagc
     1201 gatagcacct ttcgtatcac gaccgttcat agggttcgcg cccggagcga aaggagttcc
     1261 accgatacgg ccatccggtg tcgtaccagt cttcttacca tataccacat tagaggtgat
     1321 cgtaaggatg gactgcgtag gtttcgcatg gcgataggtc tcgtgctgac gcaaatattc
     1381 catgaacttc tcggtgatct ctacagcgat actatccgta cgatcgtcgt tattaccgaa
     1441 aggaacatat tctccctcac gttcgaaatc aaccgccaag ccacgctcgt cacggatcac
     1501 acgtactttc gtatcacgga tagcagccaa cgaatcggca acgatagaaa gaccggcgat
     1561 acctgtcgca cggatacgct ctacatctcc atcgtgaagc gacatctcga atgcctcata
     1621 tgcgtattta tcatgcatat aatggattat cttcaaggcg ttcacataca ctttcgccaa
     1681 ccagcgcatc atttgctcat acttagccat cacctcatcg tattccagat actcgccgcg
     1741 aaccggctcg aacatcgggg ctacctgtac gccgctgcgc tcatcccgtc ctccgttgat
     1801 cgcatataat aaacatttcg ccaaattcgc acgggctccg aagaactgca tctgcttacc
     1861 gatcttcata ggagaaacgc aacaagcgat accataatca tctccataat ccggacgcat
     1921 caaatcgtcg ttctcatatt ggatagccga tgtatcgata gagaccttgg cacagaagcg
     1981 tttccagttc tcgggagcgt tcttaaacca cagaactgtc aaattgggtt ctggtgccgg
     2041 acctagatta tataatgtat ggagataacg ataggaagtt cttgttacca aagaacggcc
     2101 atccactcct tggccgccca aggactctgt tacccaaacc ggatcaccgg agaacaaatc
     2161 attgtattcc ggcgtacgta agaaacgaac gatacgcaac ttgatgatca gctggtctac
     2221 caattcttgc gcctcctctt cggtcaactt accctcacgg atgtctttct cgatataaat
     2281 atccaaaaac gtagaagtac gtccgataga catcgccgcg ccatcttggt cttttaccgc
     2341 cgctagataa gctaagtaca caaactgaac cgcctcacga gcgttttgtg ccggacgagt
     2401 cacatcaaag ccatagctag cgcacatttt ctcgaacgct ttcaaagcct tgatctgttc
     2461 ggaaatctcc tcacggctct ggatgatctc ttccgtgatc tcttggatat ccaaacgatt
     2521 caagaaacgc tttttctcct cgatcaagat tgccgtacca tataaagcga cacgacgata
     2581 gtcaccaata atacgtcctc taccatacgc gtcgggcaga ccggtgataa tagccgaacg
     2641 acgtgcggca atcatctctt ccgtatatgc ggagaaaacg ccctcgttat gagttttacg
     2701 gtacttcgtg aagatctctt tcgtcatcgg gtccaaatga taaccataag cttccaagct
     2761 attctcaacc atacggatac cgcccttcgg gaagatacca cgtttcagcg gagcatcggt
     2821 ttgtaaccca acgatcacct cattctcctt atcaatatag cccgggccat atacatcgat
     2881 cccttgaggt aattttgtct cggcgtcata aactcccttt tccctctcga ccttaaacat
     2941 ctcggtcagc ttgttccaga ccttacgggt cttctcggaa gaagcgacta aaaaggaatc
     3001 atccccttca taaggcgtat agttactctg gataaaatct cttacattga tctctctttt
     3061 ccaggtctca cccttaaagt gatcccagca ctcattctca aatttcataa acaatcttat
     3121 ctaataccac atttttaccg ccgcaaaggt aggagaatta tcgtcatatt tctgtcacat
     3181 atgctacacc agttctaaaa tccctataaa ttgcgataaa aaagcttctc atccttcact
     3241 attttaaaag gacattacca ataaatgggg agtcagacct aagggcgaag aggcaaccat
     3301 gcccaaaaag tagatccgac accaagtttt gaagtaaagc ccacttctcc ccctgccgct
     3361 tctacgatag ctctggagat cgtcaagcca agtccggtac cctgcgagaa ctcattcaat
     3421 ttctcaaaac gggtaaatac tttgtcatgc aaatcatccg gtataccgat tcccgtatcc
     3481 tcaacataga tacgaagacc tttcctttca atgccatatc ccattttaat atatccggat
     3541 ttggtgcatt ttaccgcatt agtgaggaaa ttcatccata cttgcttaag ccggtttcta
     3601 tccagaagta cccagcactc cgagttggga ccatctaaac gtaactcgac attcggattt
     3661 gtcatcttcg cctgaatcgt aacaaataac tcattacaaa ccttagccag attgaaattc
     3721 tccttcctac gttcaattat gcctgactct atcttggaaa gatcaaggat atcgttgatc
     3781 aatcgcaata acaactcatt attggattgg atgatattta tatattcttt tttttcttcc
     3841 ggatcatcac aagttaccat taactctgaa aaaccaacaa tagcgtttaa cggggtacgt
     3901 atctcgtgac tcatattagc gagaaacgca gacttgagac gatccgaaag ttccgctttc
     3961 tcctttaatt ccttcaattg ctgaatcatc ttctcccact tcgtattatt gatcgtaata
     4021 ccggtatagc gagtcacatg gcgttcttta tctctttcaa gctggattcc agtaaccaat
     4081 aaggtctgcc actcgtcttc ccttttggtt gtggaacgat atttaacgtt gaaatcttta
     4141 tctatgcccc ggaacatatg ctccatatat ttccgcacaa aaaccacatc atccggatgt
     4201 gtagcgttca aataatcctc gggagtgatt gtcttttccg aataaaaatc attgatagga
     4261 tcattaaaag cactgaattt caggcttttt acatcaaaat cccaatacga cataccaacc
     4321 gtcttgaaag aaagctctat cttgtaattt ctttctttaa gttcccgatt caattgctgg
     4381 atcttaggaa tgttttgcct gaatcccgta tagcgtatta tatctccatt ttcatcttga
     4441 tcgaaaggga cacccataaa atcacaatac tgccaagtct catcatactt ggtttgtatg
     4501 cgacaggtaa aattgatggt gacattcttt ccagctatca gggattgcat tgcgtcgtaa
     4561 aaaacagacc ggtcctcggg gtatataaca tccatatact ccgaaacggt caataaccga
     4621 tctgaaacgt aatcgttgat cggatcatta taagattcga atttctggga atttacatca
     4681 aaatcccaat gaacaatatt actgactttc atggccagat cacgttttgc gatcagatcc
     4741 tgcgctttct tggccatttg caacctttcg gttatatcca tttgtgtccc gatgatctgc
     4801 aattttccac gatgttctgt cgataggcgc atccggctct cataataacg atacatctct
     4861 tcttgctcgt tatagaaacg caaagtcatt tgtccttgtt ctatctcatt tcctatcaat
     4921 tgagagaaga gccggattaa cggagctcga tcttggggat gcaaaatatc cagtaccgcc
     4981 tccattggca tatttttttt ggctacggtt agtccatata atggattaaa aaccttttta
     5041 ttcacatcat atacccaaga agccatatta gccgctttca tggccatctc cagatttttc
     5101 ttgctttctt ccgtacggaa ttccacctct ttcacggaag tcatatcatt tgcgagtaat
     5161 atatgtccga taagatgttt ttttctactc catatcggga taactttaac cccatagatc
     5221 atcgtgtctt tattatggga agaataatat gcatccttat taacccgatc aaaatcatac
     5281 tcaaactcca aggaaatatc ttcacccgat tgtatccgag ctaaaagttc tgaatcaaca
     5341 aatggacttt tgtaaagatt gactatattc accaccgatg tccggtcgtc cacaccatac
     5401 atcctcaacg catggtcgtt tatactacgc agcacaccat gtgcgtcata aatttcaata
     5461 cccaccggca gacaagcata tatactctcc gtaaataaac gctgttcttc tattaattta
     5521 gaaatttctt tctctattat cacatcgtca ttttcttgag aattctcttc acattgttcc
     5581 atctgtcact tactttattt cgtattatca tacgtcagac attctaattt atacgaaagc
     5641 cctgcttttt gcaaaagtaa acaaaaaagt gaattctttt ctgttcgttc ataaaga
//
