LOCUS       c00412_gnlPro..         3637 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BLHNNCLI_412.
ACCESSION   c00412_gnlPro..
VERSION     c00412_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:37:11
            Original ID  :: gnl|Prokka|BLHNNCLI_412
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3637
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3637
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[23:3494]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      24..3494
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3637
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3637
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             24..716
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg412_1"
                     /sec_met_domain="SucD_FumR (E-value: 3.1e-75, bitscore:
                     239.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSISGLFLVLFLLFHLSMNVAAVFSGEAYNMV
                     CSLLGSNWYAVAATLVLAAGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWASQN
                     MFVLGLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQGNAV
                     ITVLYLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAIITII
                     FFLNGVGA"
     CDS             765..2708
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 194.5; E-value:
                     1.1e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg412_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 5.4e-61, bitscore:
                     194.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.5e-39,
                     bitscore: 122.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHI
                     MWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             2739..3494
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg412_3"
                     /sec_met_domain="Fer2_3 (E-value: 3.3e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 6.7e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 7.1e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
ORIGIN
        1 taaataactt aaattattat cttatgtggt tacttaattc ttctatcggg aagaagctga
       61 taatgagcat atcaggtctc ttccttgttt tgttcttatt gttccacctg tcgatgaacg
      121 tggctgccgt tttctccgga gaggcttata acatggtttg ttcgctgttg ggctctaatt
      181 ggtacgctgt ggcagctact ctcgtattgg ccgcaggcgt tgtgatccac ttcgtgtacg
      241 ctatcattct taccttgcaa aaccgtaagg cacgtggtaa cgaccgttac gctatcaacg
      301 ctcgtccgaa aggcgtggaa tgggcatctc agaacatgtt cgttttgggt ttgatcgtta
      361 tcctgttcat gctgttgcac ttcagccagt tctggtataa gatgatgttc gccgaattga
      421 tcggtcatca cgaagtagca ttggggtcag ctatggtatc tccacaagac ggagccgctt
      481 ttatcaacta ttacttccaa ggtaatgccg tgatcaccgt tctttatttg atctggtacg
      541 tggctttgtg gttccacttg actcacggtt tctggagcgc tatccaaaca atcggttgga
      601 ataacactat ctggatgaac cgttgggaat gtatctctaa gatcgtagct acggttattt
      661 gcggactttt cgcgatcatt actatcatct tcttcctaaa cggagtaggt gcttaattgc
      721 aagctacagg ttttataatt aagaactttt gaaattaaat cattatgtct gaaataaatt
      781 ctaaaatacc tcaaggcccg ttggctgaga agtggactaa ttataaaaat caccagaaat
      841 tggtgaaccc cgctaacaag cgtcgtttgg atgttatcgt agtcggtaca ggtttggccg
      901 gtgcttccgc tgccgcatct ttggcagaga tgggattcaa cgtattgaac ttctgtatcc
      961 aagattctcc ccgccgtgcg cactctatcg ccgctcaagg tggtatcaac gccgctaaga
     1021 actaccagaa tgatggtgac tctgtttacc gtttgttcta cgatacgatc aaaggtggtg
     1081 actaccgtgc tcgtgaggct aacgtttatc gtttggctga ggtttctaac tcaatcatcg
     1141 accagtgcgt ggctcaaggc gttcctttcg ctcgcgaata tggtggtttg ttggataacc
     1201 gttcattcgg tggcgctcag gtatcgcgta cgttctacgc ccgtggccag acgggtcagc
     1261 agcttttgtt aggcgcttat tcagcgttga gccgtcagat cggtttgggt aaggttaaga
     1321 tgtacactcg tcatgagatg ttggatgtag ttaaggtaga cggtcgtgct cgtggtatca
     1381 tcgcccgcaa cttgattaca ggtaagatcg agcgttacgc cgctcacgct gtagttgtag
     1441 ctacgggtgg ttacgttaat acgttcttct tgtcaaccaa cgctatggca tctaacggtt
     1501 ccgccgcatg gcaatgctac aagaaaggcg cttacttcgc taatccttgt atggttcaga
     1561 tccacccgac ttgcgtaccg gtgaaaggtg acttccaatc taaattgact ttgatgtccg
     1621 aatccttgcg taacgacggc cgtatctggg ttccgaagaa actggaagac gctaaagctc
     1681 tacaggccgg aacgaagaaa ggtaaagata ttcccgaggc agaccgtgat tattatttgg
     1741 agcgtcgtta tccggcattc ggaaacttgg ttcctcgcga cgtggcttct cgtgccgcta
     1801 aagagcgttg cgacgcaggt ttcggcgtga acagcacggg cttggccgta ttcttggatt
     1861 tctccgacgc gatcaatcgc ttgggtaaag aggtcgttaa gcagaaatac ggtaacttgt
     1921 tcgatatgta cgaagagatc acgaacgata atccatacga gactccgatg atgatctacc
     1981 cggcattgca ctactcaatg ggtggattgt gggttgacta tgagttgatg acctccattc
     2041 cgggtctgtt cgctatcggt gaggcaaact tctccgatca cggagcgaac cgtttaggtg
     2101 cttccgcttt gatgcaggga ttggccgatg gttatttcgt attaccatac acgatccaga
     2161 actatttgtc cgaccaaatc caagttcctc gcttcagcac ggatctgccg gagttcgtcg
     2221 aggcagaaaa ggccatcaag gatcgtatcc aaaaattgat gaacgtcaag ggtaaagaaa
     2281 ccgtagacac tatccaccgc aaactaggtc atatcatgtg ggaacatatc ggtatggcac
     2341 gcgacgcaaa aggtttgcaa gaagctatcc agatgctgaa agacttgaag aaagagttct
     2401 ggagcaacgt attcatccca ggcaacgcag acaacttgaa taccgagtta gagaaagcat
     2461 tacgtgtagc cgactttatc gagatcggta cattgatggc acacgatgct ttggaccgcg
     2521 ctgagtcttg tggtggtcac ttccgtacgg agcaccagac agaagaaggt gaggccttgc
     2581 gtcatgacga taagttcatg tacgtatctt gctgggagta ccaaggcgag gacaaagatc
     2641 ctgtcatgtt gaaagaggat ttgaactatg agttcgttgt tcctcaaaca cgtaactaca
     2701 agaagtaata atcatcaatc tcagaaaaaa ttagaatcat ggataaagat ataagtgtaa
     2761 cacttaaggt ttggcgtcag agaggtccga aggagaaagg acagttcgag acatacaaaa
     2821 ttgagaagat caatcagagt gtctctttct tggaaatgtt ggatatcttg aacgagcagt
     2881 tggtcaacga gggtaaggaa ccgatcgtat tcgatcacga ctgccgcgag ggtatctgtg
     2941 gcatgtgttc attgtatgta aacggacacc cgcacggacc ggcaactgga gctacgactt
     3001 gccagttata tatgcgtcgt ttccacgatg gagagacgat cacgatcgaa ccgtggcgtt
     3061 ccgcaggttt ccctgttatc cgcgacttga tggtagaccg ctacgcttac gacaaaatca
     3121 tccaagcggg tggtttcaca tctgtcaaca caggtggtgt acccgacgct aatgcgatac
     3181 ctatcccaaa gaaggacgca gacttagcca tggacgctgc cgcttgtatc ggttgtggcg
     3241 cttgtgctgc cgcttgtaag aatggttccg ctatgttatt cgtatccgct aaggttagcc
     3301 agctggcttt gttgcctcaa ggtaaagtcg aggctgctcg ccgtgccaaa gctatggtcg
     3361 ctaaaatgga tgagcttggc ttcggtaact gtacgaacac acgtgcttgc gagatggaat
     3421 gtccgaagaa tatctccgtt agcaacatcg ctcgtttgaa ccgcgagttc atttgcgcta
     3481 aattacaaga ctaatatttg attagtatat catggaaaag gcgtctttct ctaaaagaaa
     3541 ggcgcctttt tgtttttgtt atatgtcgta aaacataatt tagcattata gcccttgaca
     3601 ggaattgaaa agtccgtata tttgcatcgt ggttttt
//
