LOCUS       c00255_gnlPro..         2684 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ALIJMEEH_255.
ACCESSION   c00255_gnlPro..
VERSION     c00255_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:15:35
            Original ID  :: gnl|Prokka|ALIJMEEH_255
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2684
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2684
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[886:2682]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      887..2682
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2684
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2684
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..524)
                     /locus_tag="ctg255_1"
                     /transl_table=11
                     /translation="MRPFSILAGKYLIGNSIGEGGFGITYIGMDLNLEVRVAIKEYYPQ
                     GFALRDNRTNDSTVWPCSENTQAFFEEGREKFINEARTIAKFRDLPEIVGVMDFFRENQ
                     TAYIVMEYLDGKTLKQYLAANEGRIPVSELLQMMQPLISSLEKLHDQNLIHRDISPDNI
                     MIMKKGNKKIL"
     CDS             complement(887..1624)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 82.4;
                     E-value: 1.3e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.2e-31, bitscore:
                     96.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEEIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDTW
                     KMGEGQQYTPSQLLKQALRYKNYWGNKGGITVSGGEPLLQIDFLIEFFRQAKAAGVHTT
                     LDTSANPYTEKEPFYSKWLELMKYTDLVLLDIKQIDEEEHIKLTGQSNKNILAMARKLS
                     DMGKPMWIRHVLVPGGSDKDEYLHRLADFIHTLKTVERVEVLPYHTLGVFKWEQLGIPY
                     PLEGVRPPSEERINNAREILGAI"
     CDS             complement(1627..2682)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg255_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-43, bitscore:
                     135.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.7e-96, bitscore:
                     309.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYAINGGV
                     DEKTKQQVGPQYQPITSEYLDYDEVIAKYDKMMDWLAHLYVGTLNMIHYMHDKYYYEAA
                     EMALIDTKVDRSFATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDFPRYGN
                     DDDRADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDGRKAGE
                     PLAPGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHTEEERTENLV
                     HVLDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQL
                     DVIARTCHDRM"
ORIGIN
        1 tccaaaattt ttttgtttcc ctttttcata atcataatat tatctggact gatgtctcta
       61 tgaattaaat tttgatcatg taatttttca agagaagaaa taagtggctg catcatctgc
      121 agaagttcgc ttacaggaat tcttccttca ttagctgcaa gatactgttt cagagttttt
      181 ccatctaagt attccataac aatatatgca gtctgatttt ccctgaaaaa atccataact
      241 cctacaatct ctggtaagtc acgaaatttt gcgatcgttc tcgcttcatt gataaatttc
      301 tcacgacctt cttcaaaaaa cgcttgtgta ttttcagaac acggccatac cgtactgtca
      361 tttgtcctgt tgtccctaag agcaaatccc tgtggataat attctttgat cgcaaccctt
      421 acttctaaat taagatccat tcctatgtag gtaattccaa aaccaccttc gccaatagaa
      481 ttaccaatta aatattttcc agcaagaata ctaaaaggtc tcatatgatt tcttggaaga
      541 ttaaaagttc tgatatcaaa tccacaaaat tcacatgccc catcagggtt tctcatttct
      601 cgcatgcagt tcgcacatag gttctctatt ttcacacatt ctcctcctgg cttttcttaa
      661 attttgtgac cgacattcgt gtcggtcgca aaagtgcttt cataactttc tagaagtttt
      721 tacaatttcc tgcttccatc tccttaaaac gggtaacaca ctgttattat aacataacac
      781 aaaaatataa atacttctac ctgtccccaa acttctggtc ataaaaaaag atctctctgc
      841 cataactggc attgagatct ttttcttttc cagtcctctg accggtttat atcgctccca
      901 gaatttctct ggcgttgttg atccgttcct ctgatggtgg tctgacacct tccagcggat
      961 aaggaattcc gagctgttcc cacttaaata caccaagtgt gtggtacgga agcacttcga
     1021 ctctctccac tgtttttaaa gtatggataa aatctgcaag cctgtggaga tactcatctt
     1081 tatcgctgcc tcccggaacc agtacgtggc gaatccacat cggttttccc atatctgaca
     1141 atttccgtgc cattgccaga atatttttgt tggactgtcc tgtaagcttg atatgttctt
     1201 cttcgtcaat ctgcttgata tccagaagaa caagatcagt atacttcata agctccagcc
     1261 atttggaata aaaaggttcc ttttctgtgt aaggattggc actggtatca agagtcgtat
     1321 gaactcctgc ggcctttgcc tgacggaaaa attcaatcag gaaatcgatc tggagaagag
     1381 gttctcctcc actcacagtg atcccgcctt tgttacccca gtaattctta tagcgaagag
     1441 cctgtttcag aagctgagac ggggtgtact gctgcccctc tcccattttc caggtatccg
     1501 gattatggca gaactggcag cgcattgcac atccactcag gaatatcaca tatctgacgc
     1561 ccggtccgtc tactgatccg aagctttcca gagaatggac atatcctttg atttcttcac
     1621 tcattattac attctgtcgt ggcaggttct ggcgataaca tccagctgct gctctcttgt
     1681 aaggtcgatg aatttaactg catatccgga tacacggatt gtgaagtttg cgtactcttc
     1741 tttttccgga tgttccattg catcgatcag tttttcttta ccaaatacat taacgttgag
     1801 gtgatgagca ccctggtcaa agtatccgtc aagtacatga acaaggttct ctgtacgttc
     1861 ttcttctgta tgtcccagag catccgggtt gatggtctga gtattggaga ttccgtcaag
     1921 ggcatcttca tagtccagtt ttgcaacaga gttgagggaa gcaagaagtc cgttctgctc
     1981 tgctccgtag gacgggttgg caccaggtgc cagcggttcg cctgcttttc ttccatccgg
     2041 aagggatcct gtagctttac cataaacaac gttggatgtg atggtaagga tggatgtggt
     2101 aggcttggaa tcacgatagg tatggatgtg tttcagtttc tcaaggaatg tggaaagcag
     2161 tgttgttgcg atctcatctg ctctgtcatc atcgttacca tagcgtggga agtcaccctc
     2221 tacctggaag tctgttgtaa ttccgtcttc atcacggata gctttaactt ttgcatattt
     2281 gatagcggac agggagtcta ctacgtgaga gaatcctgcg ataccagttg cgaaggaacg
     2341 gtcaaccttt gtatcgatca gagccatctc tgctgcttca tagtagtatt tgtcatgcat
     2401 gtagtggatc atgttcagtg ttccaacata cagatgagcc agccagtcca tcatcttgtc
     2461 atattttgcg attacttcgt catagtcaag gtattcagaa gtaattggct gatactgagg
     2521 tcctacctgc tgttttgtct tctcatcaac accaccgttg atagcgtaca gaaggcattt
     2581 tgcaaggttt gcacgagctc cgaagaactg catttcttta cctgtctcag tagcagatac
     2641 acagcagcag atgctgtagt catcgcccca taccggacgc ataa
//
