LOCUS       c00071_gnlPro..         3393 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNGIGBFC_71.
ACCESSION   c00071_gnlPro..
VERSION     c00071_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 17:56:06
            Original ID  :: gnl|Prokka|PNGIGBFC_71
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3393
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3393
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2792:3392](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2793..3392
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..3393
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3393
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             complement(17..721)
                     /locus_tag="ctg71_1"
                     /transl_table=11
                     /translation="MALPIAIVEDQTPDAQRLEELLQQQLPAAECTWFACGDDFLRAAA
                     EPGLYAVVFLDICMAGTNGIETARRLRQADPEVLIVFVTSSPEYVWDAFPVHPFDYLLK
                     PYQEEKFVHLAAELRRALRCQEPELEIRIARQSIQLPLRKVLYAIARNHYVLITTEDGE
                     YRAMCSFAQIEEKLTALENFMSCNRGVIINMDKVLRFGEDCIEILDGTRLPVRQKDKCT
                     LLAGTMSSMKQK"
     CDS             1314..2780
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_7_arginine"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg71_2"
                     /sec_met_domain="OKR_DC_1_C (E-value: 1e-12, bitscore:
                     36.3, seeds: 113, tool: rule-based-clusters)"
                     /sec_met_domain="OKR_DC_1_7_arginine (E-value: 6.6e-124,
                     bitscore: 401.1, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNENKARSRLDQRRAPIYEALERFRQMRVVPFDVPGHKRGRGNPE
                     LTAFLGQQCVGVDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSSVQS
                     MVLTACKRGDEIILPRNVHRSVLNALVLCGAVPVYVNPEVDKRLGISLGMKREQVAKAI
                     KEHPNAVAVLVNNPTYYGICSDLRAIVKMAHDAGMLCLADEAHGTHFYFGGGLPVSAMA
                     AGADMASVSMHKSGGSLTQSSLLLIGPNVHPGYVRQIINLTQTTSGSYLLMSSLDISRR
                     NLALRGRQVFHQVADIAEYAREEINAVGGYYAFGKELCNGDSVFDFDTTKLSVHTLDIG
                     LAGIEVYDILRDEYDIQIEFGDIGNILAYLSIGDRPQEVERLVSALAEIKRRYRTDGSG
                     LLSQEYIDPVVAASPQEAFYAPKKSLPLRETEGMVCSEFVMCYPPGIPILAPGERITKE
                     ILNYIEYAKAKGCSMTGPEDPEILHLNVLA"
     CDS             2793..3392
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_3"
                     /sec_met_domain="Spermine_synth (E-value: 4.1e-50,
                     bitscore: 157.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 3.9e-24,
                     bitscore: 72.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFWFSEFHTPDVKHSIRVNKQLYSKQSDYQRIDIFETPEFGRVL
                     TLDGNVMLTERDEFIYDEMIVHVPMAVHKEAKDILVIGAGDGGVVRELTRYDRVERIDL
                     VEMDPQVVEACRAYLPGNACRMDDRRVHIYFENALKYIRRCEEEYDLIIVDSSDPFGPS
                     EGLFTREFYGSCFNALKEDGIMVNQQGSPFYAEDASA"
ORIGIN
        1 accgtctttg atgaaactat ttttgtttca tcgatgacat tgtaccggca agcagagtgc
       61 atttatcctt ctgccgtacc ggcaggcggg tgccgtccaa gatctcaatg cagtcctccc
      121 cgaaccgcag caccttgtcc atgttgatga taacgccccg gttgcagctc ataaagtttt
      181 ccagagcagt aagcttttct tcgatctgcg caaagctgca catggcacgg tactcaccat
      241 cctcggtggt gataaggacg tagtggttcc gggcaatggc atacagcacc ttgcgcagcg
      301 gcagctgaat gctttggcgg gcaatgcgga tctccagttc cggctcctga caacgcagcg
      361 cccgccgcag ctcggcggca aggtgcacga atttttcctc ctgatagggt ttgagcaggt
      421 aatcgaaggg gtgcaccgga aaggcatccc atacatattc cggcgaggag gtcacgaata
      481 cgatcagcac ctccgggtcg gcctgccgca gtcggcgggc ggtctcgatg ccgttggtgc
      541 ccgccatgca gatatccaga aacaccacgg cgtataagcc gggctctgct gcggcacgga
      601 gaaaatcatc cccgcaggca aaccatgtgc actcggctgc aggcagctgc tgttgcagca
      661 gctcctccag ccgctgggcg tccggggtct gatcctctac gatggcgatt ggcagtgcca
      721 tccatacacc ctctttctcc attcattgtt cctttattat agcatactgc ggcgggaagg
      781 gtatctgctt ttttgcacaa aacgccccat tttgcgcgcc gagcgccgcc ctgcgcaccc
      841 gcagcgggcg actgccaaaa agttttggtt gacagacggg gcgaatcatg ttattctttt
      901 gttgattttg agagacgccg ccttgcagag tcttttcaaa gcaatttact cgggaccgcc
      961 caaacggggc taaggccata cggacagccg ggtccactgc cgaccggcgg cgcaagctgc
     1021 cattattgat tgagtttgaa gctcaaattt tataagttgg ttcctttaca accatgaaat
     1081 tgcgcagcct atgcgcagac ttgtgaaacg gtcaataaga gtagtaaaag tgtagttgct
     1141 atatagactc tcatcacccc gtctttttgg atcgcgaata acgcgcagca ccggcactta
     1201 cggctgggtg ctttatcagc gcggaaaaag ctgctccaag cgcaggtctg catatttgtg
     1261 cagtgtctgc gcttttttgt tgtgcaaaaa gaagaacagg gacggaggaa aagatgaacg
     1321 agaacaaagc gcgttcccgg ctggatcagc gccgtgcgcc catttacgag gcgctggagc
     1381 ggttccggca gatgcgggta gtgccttttg acgtgcccgg ccacaagcgg ggccgcggca
     1441 acccggagct gaccgctttt ctgggtcagc agtgcgtggg cgtggacgtg aacagcatga
     1501 agccgctgga taacctttgc cacccggtgt cagtcatccg ggaagccgag gagcttgccg
     1561 ccgatgcctt tggcgcagca cacgcctttt taatggtggg cggcaccacc agcagcgtgc
     1621 agagcatggt gcttaccgcc tgcaagcggg gcgatgagat cattctgccc cgcaacgtgc
     1681 accgcagcgt gctgaacgcg ctggtgctgt gcggcgctgt gccggtgtat gtgaacccgg
     1741 aggtggataa gcgtctgggc atctcgcttg gcatgaagcg ggagcaggtg gcaaaggcca
     1801 tcaaggagca ccccaacgct gtggctgtgc tggtgaataa ccccacctac tacggcatct
     1861 gctccgacct gcgtgccatc gtaaaaatgg cgcacgacgc cgggatgctc tgccttgcgg
     1921 atgaagccca cggcacccat ttctactttg gcggcggctt gccggtatct gccatggcgg
     1981 cgggcgcgga tatggcctcg gtgtccatgc acaaaagcgg cggcagcctg acccagtcca
     2041 gcctgctgct catcggtccc aacgtgcacc cggggtatgt gcgtcagatc atcaacctga
     2101 cccagactac ctccggcagt tacctgctga tgtccagtct ggatatctcc cgccgcaatc
     2161 tggcgctgcg gggacggcag gtgttccatc aggtggcgga tattgccgag tatgcccgcg
     2221 aggagatcaa cgccgtaggc ggctactacg cctttggcaa ggaactgtgc aacggcgatt
     2281 ccgtctttga tttcgacact accaagctca gcgtccatac gctggatatc ggtcttgcag
     2341 gcatcgaggt ctacgatatc ctgcgggatg agtacgatat tcagatcgag ttcggcgata
     2401 tcggcaacat ccttgcctac ctgtccatcg gcgatcgccc gcaggaggtg gagcgtctgg
     2461 tcagtgcact ggcggagatc aagcgccgct accgcaccga tggctcgggt ctgctgagtc
     2521 aggaatacat cgaccctgtg gtggctgcca gcccgcagga ggctttctac gcccccaaaa
     2581 agagcctgcc cctgcgggag accgagggca tggtgtgcag cgagttcgtg atgtgctatc
     2641 ccccgggcat ccccatcctt gcacccggcg agcgcatcac caaggagatc ttgaactaca
     2701 tcgagtacgc caaggccaag ggctgcagca tgactggccc ggaggacccc gaaatcttgc
     2761 acctgaatgt tctggcataa ggagggaaag agatggaatt ttggttttca gagtttcaca
     2821 ccccggatgt gaagcacagc attcgtgtaa acaagcagct ctactccaag cagagcgatt
     2881 atcagcgcat tgatatcttt gagaccccgg agttcggccg ggtgctcacg ctggacggca
     2941 acgtgatgct gaccgagcgc gacgagttca tctacgatga aatgatcgtc catgtgccca
     3001 tggcggtgca taaggaggct aaggatattc tggtcatcgg cgccggagac ggcggcgtgg
     3061 tgcgggagct gacccgctac gaccgtgtgg agcgcatcga ccttgtggag atggacccgc
     3121 aggtggtgga ggcctgccgc gcctacctgc ccggcaatgc ctgccgtatg gatgaccgcc
     3181 gggtgcatat ctactttgag aacgccctga agtatatccg ccgctgcgag gaggagtacg
     3241 acctcatcat cgtggactcc tccgacccct tcggcccctc tgagggtctg ttcacccgcg
     3301 agttctacgg cagctgcttc aacgccctga aagaggatgg catcatggtc aaccagcagg
     3361 gcagcccctt ctacgccgag gacgccagcg cca
//
