LOCUS       c00063_gnlPro..        14792 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NCNKIAFH_63.
ACCESSION   c00063_gnlPro..
VERSION     c00063_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:57:16
            Original ID  :: gnl|Prokka|NCNKIAFH_63
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14792
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14792
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[6793:12264]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      6794..12264
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..14792
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14792
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             3..2009
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CPSase_L_D2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg63_1"
                     /sec_met_domain="CPSase_L_D2 (E-value: 7.7e-43, bitscore:
                     136.2, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EQHVDSLMSYDFTGLSREELVKQLHVVDDRRIWVIAEALRRGMDY
                     DVIHDITKIDKWFIDKLAILVEMEQKLKSESLTVELLKEAKRIEFPDKVIAELTGKTET
                     EIHDMRYANGIVAAYKMVDTCAAEFEATTPYYYSVFGSENEAEETHPNKKVLVLGSGPI
                     RIGQGIEFDYCSVHCTWAFAKEGWETVIVNNNPETVSTDFDIADKLYFEPLTPEDVEAI
                     VKLEKPDGAVVQFGGQTAIKLTESLMKMGVKILGTKAEDVDAAEDRELFDEILEKTKIP
                     RAAGGTVFTAEEAKKVANRLGYPVLVRPSYVLGGQGMKIAFNDDEIEEFIGIINRIAQD
                     HPILVDKYLQGKEIEVDAVCDGTDILIPGIMEHIERTGVHSGDSISVYPAPTISDHVKE
                     TIVEYTKRLAQALHVIGLINIQFIAMNEEVYVIEVNPRSSRTVPYISKVTGIPIVDLAT
                     KVIIGNTIRGLGYEPGLAPTADYIAIKMPVFSFEKLRGAEISLGPEMKSTGECLGIAKT
                     FNEALYKAFLGAGVTLPKYKQMIMTVKDADKPEAVGVAKRFEALGYKIYATRSTAKYLQ
                     EHGVNALRVNKITQESPNVMDLILGHKIDLVIDTPTQGNGDKTRDGFLIRRNAIETGVY
                     CITAMDTANALARSLETAMDTLTPVDIAKVKNL"
     CDS             complement(2269..2415)
                     /locus_tag="ctg63_2"
                     /transl_table=11
                     /translation="MNKQKCTTSIKEEIIMLAKIKAFFMENIDGVAMGVEAMYGVDIHP
                     YYN"
     CDS             2520..3389
                     /locus_tag="ctg63_3"
                     /transl_table=11
                     /translation="MEEQKNTYENVTLEPDVGFDIRFVTMDHNSPFHWHREMEILYILN
                     GHATVHMEGEEYELNPLDAVVMDYARIHEVIYALPQTMGICIHVSRQLLRRYLPEAEPF
                     AIRCAGQRLRPGQQEYYNEICGYLKELTVLYVNQNETYRLKSTARILELLACLVEHFSE
                     PVSMVATQTRAGKMERLEQICMYVDHHYREEMTLQEAADELGLNREYFCRFFKQSTGSS
                     FMRYVNQVRLNYIYQDLLHTDDPVQEIMERHGFFNQKLFYRMFKERYHCTPRQARRMAE
                     NNPYVEAK"
     CDS             complement(3451..3609)
                     /locus_tag="ctg63_4"
                     /transl_table=11
                     /translation="MFCSAMTVRDTHESPKIVCNLRTHLVEYSKLGKTRIIENVKHDAA
                     RNAGFGG"
     CDS             complement(3907..5244)
                     /locus_tag="ctg63_5"
                     /transl_table=11
                     /translation="MTIPEEGEIIMQFVKTADLKPGMRLAKPIYNRMGVLLYERDTRLT
                     IQGINSIENFGLIGIFILEPAEPLPPLSREDLEFEQFQTIYMFKLKDNMDKLVDNLVPS
                     TLLSLVDDIQRHYGSLDHKLNFTQTLRSSADFVYKHSISVGILSAMISNEMQLPAEDIR
                     ALITASLLYDFGYLYVPQAILDKGDDLSDSDRNFIQMNLERGYESIRPRYEECNLPKIS
                     LEIIQQFIFQKSQTLKIKDPSPETRLLCDILKVADQFDRLTAMNINNPPVSEVAAMSFL
                     RRHSRTYNPRVVAALAECIHILPTGACVDLSDGEKALVLVENAADFTRPMILKFSNNMI
                     YDLSDPVIGDSLRVTDIMKTMDNRIAIDKEALEHFVADQYIRETADRFRQKKLAIAQRK
                     QKAAQKKSMDDLLDNARVLTPPPIAPVPEEDASPIRKAPRKRMKLV"
     CDS             5306..6523
                     /locus_tag="ctg63_6"
                     /transl_table=11
                     /translation="MILLEPGFLKENPTFPEKEVLMIQIPWRRVAGAALVMCLLLANTS
                     YAETTQDNINDAKNQIEDLKDQKKDAEDTVNDISGKKDALESDLSGLNGQLSDIVAQIN
                     DLEGQISDKQGEIDQAKEDLAAAEAQSAKQYEDMKLRIQFMYENGSTPVWQMLAEAESF
                     SDFLNRTEYITDINAYDRKKLVEYQDLQEQIAAQKEDLESDMTELVAMQDDMKKKQANV
                     SSLINTTKANLAQTQEDLADAQSNVADLEDKINQMVEYEKQLEIQKAKEDAARLAAIKE
                     QEKEDTSTVVYVPTDSDQYLLGAIIQCEAGGESYDGKLAVGSVVINRVKSSYFPNSVSG
                     VIYQSGQFSPVASGRLAYRLEAGVDGSCLQAAQDVLNGNITVGCLYFRQNNGIIQGTVI
                     GNHVFY"
     CDS             6794..8158
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_7"
                     /sec_met_domain="Thiolase_N (E-value: 2.5e-98, bitscore:
                     318.3, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 7e-52, bitscore:
                     164.1, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLVKKRGRNINFNCKVEFIFFSKKIVKKLTAFFGKIIFKEEQIP
                     RTYGVLCVKLKGEFKMAKKVVLAGACRTAIGKMGGALSNTPAAELGSIVIKEALNRAGV
                     KPEQVDEVLMGCVIQAAQGQNVARQASIKAGLPIEVPAVTLNVVCGSGLKCVNEAATMI
                     LAGQADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITA
                     ENVCEKYGLTREELDEFSANSQQKCEKAIAEGKFDDEIVPVPVKVKKETVMFAKDEGPR
                     PGTTAESLSKLKCCSGKEGGLVTAGNASGINDGAAAIVVMSEEKAKELGVKPMATWVAG
                     ALGGVEPEIMGVGPVASTRKVLERTGLTIDDMDLIEANEAFAAQSVAVARDLKFDMSKV
                     NVNGGAIALGHPVGASGCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVERD"
     CDS             8310..9182
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_8"
                     /sec_met_domain="HCDH (E-value: 2.2e-35, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 1.2e-61, bitscore: 197.5,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVDGYEVALCDIKQEWADGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFEDMGVKKTTFQELDK
                     IAKPECIFASNTSSLSITEIGNGLTRPMVGMHFFNPADRMKLIEVIAGVNTPEETVEAI
                     KKISVEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             9214..10368
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_9"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 6.1e-53, bitscore:
                     168.9, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2.5e-31, bitscore:
                     97.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 8.9e-41, bitscore:
                     129.0, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFREFAENEVKPLAQEVDETEEFPVENVKKM
                     QKLGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVIVSAHTSLCIDPIMTYGTE
                     DQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSVTEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGVEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRTIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             10414..11196
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 159.3;
                     E-value: 3.1e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_10"
                     /sec_met_domain="ETF (E-value: 6.5e-50, bitscore: 159.3,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLTIMNPDDKAGLEAA
                     LRLKEQYGAEVTVLTMGLPKADEVLREAMAMGADKGILVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAQDIKIDGDYVVVQRQYEDRY
                     HELKAKMPCLITALSELNQPRYMTPGGIFDAYDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVRKGAGEKVVLDPDESVAYLIGKFKEKHII"
     CDS             11224..12264
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 163.5;
                     E-value: 1.6e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg63_11"
                     /sec_met_domain="ETF (E-value: 3.4e-51, bitscore: 163.5,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 8.8e-39, bitscore:
                     121.4, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLEEYKGVFVFAQQVDNVLDGVAFELLGKGKELAKDLNTEVTAV
                     LIGSGVKELADQLAEYGADRVIVVDDPQLKDYRTEPYAHALASVINEYKPEIMLVGATA
                     IGRDLGPTVSARVKTGLTADCTMLEIGDFPLNAAAGQEQQHNQLLMTRPAFGGNTIATI
                     ACPYNRPQMATVRPGVMQKIAPIAGAKANVVEYNPGFTPNDRYVEILNIVKAVKNTANI
                     MDAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQTGKTVR
                     PQIYFAIGISGAIQHVAGMEDSDLIVAINKDEDAPIFDVADYGLVGDLNKIVPALTTAL
                     KAELGK"
     CDS             12405..13769
                     /locus_tag="ctg63_12"
                     /transl_table=11
                     /translation="VRQTVTENRLFSKKDLRKLIIPLILEQTLAITVGMADTMMISSAG
                     EAAVSGVSLVDMFNNLIISVLAALATGGAVVTSQCIGAGRREEACRSARQLVFTEAAIT
                     IGISVLVLLFHRQILGLFFGQIEADVMQNAIIYLIISVFSFPLLAVYDSCAALYRSMGN
                     AQITLKISLLMNVINVVGNAIGVYVLKLGVAGVAIPSLVSRGVAGVVLFTLLHNPDNLV
                     FLTRGKFKVDATIVKRILFIGIPSGIENGIFQLGRVLVVSIIAAFGTSQIAANGVANSL
                     DSMGCIVGQAMSLAMITVIGRCVGAGEEGQVRYYTKKLLGETYFYTAVINSIILLLLPW
                     ILQIYGLGEETTRLSYILVMIHDGMAIFLWPASFVLPNMLRACNDVKYTMVVSIFSMIT
                     FRIGFSYVFGVHMGWMAVGVWAAMVIDWVFRVLCFVGRYLAGTWRKKCGLVVPTA"
     CDS             13864..14454
                     /locus_tag="ctg63_13"
                     /transl_table=11
                     /translation="MTKSKFSTKYLVEMALLVAIILLMAFTPIGYIKTAGLEITLIVIP
                     VAVGAVTLGPAAGAILGGVFGITSFIQCFGMSAFGTLLLGVNPVFTFLVCVPTRILDGW
                     LTGLIYAGLKKTKIPSAGSVTLASLCCPLLNTTFFMGTLVTLFAGTMREQFGMTKVIPF
                     IAAFVGVNGVVEAIVCFVIGTAVSMALKKALRY"
ORIGIN
        1 tagagcagca tgtggacagc ctgatgtcct atgattttac cggactttcc agagaagaac
       61 tggtaaaaca gcttcacgtt gtggatgatc gtagaatctg ggtgattgca gaggcattgc
      121 gccgcggtat ggattacgat gtgattcacg acatcaccaa gattgataag tggttcatcg
      181 acaagctggc aatcctggtt gagatggagc agaagttaaa gagcgagtct cttacggtag
      241 aactgttaaa agaggcaaaa cgaatcgagt tcccggataa ggtgatcgca gaattgaccg
      301 gtaagaccga gacagagatc cacgatatgc gctacgctaa cggtatcgtg gcagcataca
      361 agatggtaga tacctgtgcg gcggagtttg aggcgaccac accgtactac tattccgtat
      421 tcggtagcga gaatgaggcg gaagagacgc acccgaacaa gaaggtgctg gtacttggtt
      481 ccggaccgat ccgtatcgga cagggaatcg agtttgatta ctgctccgta cattgtacct
      541 gggcattcgc aaaagaaggc tgggagaccg tcatcgtcaa caacaacccg gagaccgtat
      601 cgacagactt tgatattgcg gataagctgt actttgagcc gctgacgcca gaggatgtgg
      661 aggcgatcgt aaaacttgag aagccggacg gcgcggtggt gcagtttggc ggacagaccg
      721 cgatcaagct gactgagagc ctgatgaaga tgggcgtgaa gatcctcgga accaaggcgg
      781 aggacgtcga cgctgcggag gatcgtgagc tcttcgatga gattcttgag aagacaaaaa
      841 ttccgagagc agcgggcgga acagtattta ccgcggagga agcgaagaaa gtggcgaacc
      901 gtctgggata tccggtgctt gtgcgcccgt cctatgtact cggcggacag ggtatgaaga
      961 ttgcgtttaa cgatgacgag atcgaggaat tcatcggcat catcaaccgg attgcacagg
     1021 atcacccgat tctcgttgat aaatatctgc agggcaagga gatcgaggtc gatgccgtat
     1081 gcgacggaac ggacatcctg attccgggta tcatggagca tatcgagaga accggtgtcc
     1141 attcgggaga cagtatttcc gtctatccgg caccgacgat cagcgaccat gtaaaagaga
     1201 cgattgttga atatacgaag cgcctggcac aggcactgca tgtcatcgga ctgatcaaca
     1261 tccagtttat tgcgatgaat gaagaagtct atgttattga ggtaaacccg agatcttcaa
     1321 gaaccgtgcc gtacattagc aaggtgaccg gtattccgat tgtcgatctc gcaacgaagg
     1381 tcattatcgg aaacacaatc cgcggactgg gctatgagcc gggacttgca ccgacggctg
     1441 attacattgc catcaagatg ccggtattct cgtttgagaa gctgcgcgga gcggagattt
     1501 cccttggacc ggagatgaag tccacgggtg agtgccttgg tattgcaaag acattcaatg
     1561 aggcattgta taaggcgttc cttggcgccg gcgtgacact gccgaaatat aagcagatga
     1621 tcatgacggt aaaggatgcg gacaagccgg aggccgttgg cgttgcaaag cgttttgaag
     1681 cgctcggcta caagatctac gcgaccagaa gcaccgcaaa atatttgcag gagcatgggg
     1741 tgaatgcgct gcgtgtcaat aagatcacac aggaatcccc gaatgtcatg gatctgattc
     1801 ttggtcataa gattgatctt gtgatcgata ccccgacaca gggcaatgga gacaagacgc
     1861 gcgacggctt tttgatccgc cgaaatgcga tcgagaccgg tgtgtactgt atcacagcga
     1921 tggatacggc aaacgcactt gcaagaagcc ttgagacagc gatggatacg ctgactccgg
     1981 ttgatattgc gaaagtgaag aatttataat tataaacaaa aaaagaatct cgcttgcgag
     2041 attctccgcg cgcgagcggt tcgcaaagcg atattttcca taccgcgagc tgcgcatccg
     2101 cgcggagcga ttcttatttc ataggaagga cctactttcg tacattaatg tgtcaaggta
     2161 tttcttatga aataagaaag agcctgtata cggatggatg agaactcatc gcaccgttac
     2221 aggctctttt ttggtaaaat tttctgttgt catggcgttg ctccgggatt agttgtaata
     2281 tggatgaatg tcaactccgt acattgcttc aacacccatt gcgactccgt cgatattctc
     2341 catgaagaat gctttgattt ttgctaacat aataatttcc tcctttattg aagtggtaca
     2401 tttttgcttg ttcatcaaag gttttccctt tgacagctat tataatacac ggaaatatgg
     2461 taatattata gataagatat tagcaaaaag ttgggtgata ttgactttta ggagaaaaaa
     2521 tggaagaaca gaaaaataca tatgaaaatg ttacgctcga accggacgtc ggctttgata
     2581 tccggtttgt tacgatggat cataactctc cgtttcactg gcaccgggag atggagattc
     2641 tctacatatt aaacgggcat gccactgtgc atatggaggg tgaggaatac gagctgaatc
     2701 cgttggacgc ggttgtgatg gactatgcca gaattcatga agtcatctat gcgctgccgc
     2761 agacaatggg aatctgtatc cacgtgtcaa ggcagcttct gcggcgctat ctgccggagg
     2821 cggaaccttt tgcgatccgc tgcgcgggac agcgtctccg tccagggcag caggaatatt
     2881 acaatgaaat ctgcgggtat ttaaaagaac tgacggtgct ttatgtgaat cagaatgaga
     2941 cgtaccggtt aaaaagcacg gcgcggattc tggaattgct ggcatgtctg gtggaacatt
     3001 tttcggaacc tgtctccatg gtggcaacgc agacgagggc ggggaagatg gagcggctgg
     3061 agcagatctg tatgtatgtg gatcatcact acagggaaga aatgacgctg caggaagcgg
     3121 cggatgaact ggggctcaac cgggaatact tctgccggtt ctttaagcag agcaccggca
     3181 gctcgtttat gcgctatgta aatcaggtgc ggctcaatta tatctatcag gatctgctgc
     3241 acacggacga tccggtacag gagatcatgg agcggcatgg cttctttaat cagaagcttt
     3301 tttaccggat gtttaaggag cgttaccact gtactccgcg ccaggcgcgc cggatggcgg
     3361 aaaacaatcc ttatgtggaa gcaaaataac tgcgctgtat atgcaggggg agaggatata
     3421 agtttcggat gcagcccggt gtgaagcatc ctaaccgcca aatccggcat ttctagccgc
     3481 atcgtgtttg acattctcga tgatccgtgt ctttccaagt tttgagtact ccacgaggtg
     3541 tgtccgcagg ttgcagacaa ttttaggact ttcgtgtgtg tcccttactg tcatcgctga
     3601 acaaaacata accgcgcgaa gccgatgtct gagcaggtat ctgccgtgtc ttcaatagca
     3661 gatgcctgcg agtttcggtt cgcgcggttt tatattgtct gtgaagcgat gacattaggg
     3721 acaccaggaa gtccgctcat tgtctgcaac ctgtcggaca catcctgtgg attgtcaaaa
     3781 cgtggcaagg acacagaata aaatgtcaaa cacgacgcgg aagaaatgct cgcattatgc
     3841 ttcaaggatg cttcacaccg ggctgcatcc ggaacgatac gccgttccca cccttcacat
     3901 aaaatattaa accagtttca tgcgcttgcg gggagctttc ctgatcgggg aagcgtcctc
     3961 ttccggtacc ggtgcaatgg gcggaggtgt gagcactctg gcattgtcca aaagatcatc
     4021 catgctcttc ttctgggcag ctttctgctt gcgctgtgcg atggcaagct ttttctgccg
     4081 gaaacggtca gccgtctcac ggatgtactg gtctgcgaca aaatgctcga gcgcctcctt
     4141 gtcgatcgca atccggttgt ccatagtttt catgatgtcc gtcacacgca gggaatctcc
     4201 gatgaccgga tcactcagat cgtagatcat attgttggag aactttaaga tcatcgggcg
     4261 ggtaaaatcc gcggcatttt ccacgaggac gagcgccttc tcaccgtcgg agagatcaac
     4321 gcacgcgccg gtcgggagaa tgtggatgca ttccgccagt gcggcaacga cgcgcggatt
     4381 gtaggtccgg ctgtgtctgc gcaaaaaaga catcgcggca acctccgaga ccggcggatt
     4441 gttaatattc atggcggtaa gccggtcaaa ctgatctgcc acctttaaaa tatcacagag
     4501 cagacgtgtc tccggggaag gatccttgat ctttaaggtc tgactcttct ggaagatgaa
     4561 ctgctggata atctctaagg aaatcttcgg aaggttgcac tcctcatagc gcgggcggat
     4621 tgattcataa ccgcgttcca gattcatctg gataaaattc cggtcgctgt ccgagaggtc
     4681 gtcgcccttg tcgaggatgg cctgtggaac atacagataa ccgaaatcat acagcaggga
     4741 agccgtgatc agcgcccgga tatcttctgc cgggagctgc atctcattgg agatcattgc
     4801 ggataggata ccgacactga tcgagtgttt gtatacaaag tccgcggaac tgcggagtgt
     4861 ctgcgtaaaa ttcagcttgt ggtcaagact gccgtaatgc cgctggatat catcgaccag
     4921 cgagagcagt gtggacggga caagattgtc gacaagcttg tccatgttgt cctttaactt
     4981 gaacatgtag atggtctgga actgttcaaa ctccagatct tcccgcgaaa gcggcggcag
     5041 cggttcagcc ggctccagaa taaaaatgcc gatcagacca aaattctcaa tactgttaat
     5101 accctggatc gtgagccttg tatcccgttc gtataacaga acccccatcc tgttgtagat
     5161 tggctttgca aggcgcatac cgggctttag atcagcagtt ttgacaaatt gcatgattat
     5221 ctccccctct tccggtattg tcataccaaa atcctcttta tcttccctct atgatagcat
     5281 attgaaagct gaaatacaat ctgatatgat actcttagaa ccggggtttt tgaaagaaaa
     5341 tcctactttt ccggagaaag aggtacttat gatacagatt ccgtggagac gcgtggcggg
     5401 agcggcgctg gtgatgtgcc tgcttctggc aaatacatct tatgccgaga cgacacagga
     5461 caacataaat gatgcgaaga atcagattga ggatctgaaa gatcagaaaa aggatgccga
     5521 ggataccgtc aatgacatct ccggcaaaaa ggacgcactg gagtcggatc ttagcgggtt
     5581 aaacggtcag ctttccgata ttgtcgcaca gatcaatgac ctggaggggc agatttcgga
     5641 caaacagggg gagattgacc aggcgaagga ggatctggct gcggcggagg cacagtctgc
     5701 gaaacagtat gaggatatga agctgcggat tcagttcatg tatgagaacg gaagcacgcc
     5761 ggtatggcag atgcttgcgg aggcggaatc tttttccgac ttcctgaacc gcacggaata
     5821 tattaccgac atcaacgcct acgacagaaa aaagcttgtg gaataccagg atctgcagga
     5881 gcagatagcc gcccagaagg aagatctgga gagtgatatg accgagctgg tggcgatgca
     5941 ggatgacatg aagaaaaagc aggcgaacgt cagcagtctc atcaacacca ccaaggcgaa
     6001 tctggcgcag acacaggaag atctggcgga tgcgcagtcc aacgtcgcgg acctggagga
     6061 taagatcaac cagatggtcg agtacgagaa gcagttggag atccagaagg cgaaggagga
     6121 cgctgcgcgt ctggcggcga ttaaggagca ggagaaggag gatacctcca ccgtggtcta
     6181 tgtgccgacg gacagtgacc agtacctgct tggagcaatc atccagtgcg aggcgggcgg
     6241 agagtcctac gacgggaagc tcgcggtcgg aagcgtcgtg ataaaccgtg tgaagagttc
     6301 gtattttccg aactctgtct cgggtgtgat ttaccagagc ggtcagttct cgccggtggc
     6361 gagcggcaga ctggcgtatc gtctggaagc gggtgtggac ggaagctgcc ttcaggcggc
     6421 gcaggatgtg ctaaacggca atattacggt gggctgcctg tatttcagac agaataacgg
     6481 catcatacag ggaaccgtaa ttggaaacca tgtattctat tagaagtgaa aaaagtgcgg
     6541 aaaaccgcag aatggcagca gggcgatttt tgtgaaaagt acacaagaat cgctttgctt
     6601 ttgtttggaa aataaaattt gcatgataaa tttttaacaa aaaaagactg taaaacagac
     6661 gtatagaggg ggaaaaagga gcgtaaagaa aactttagaa agatgaaaaa agaagaaagt
     6721 tattgcattt aagagtgaaa tatggtagca tgaaaaagta ttaaaaagct agtaaaaacg
     6781 ccgattattt aaaatgcacc ttgtgaaaaa aagagggaga aacataaatt ttaactgcaa
     6841 agttgaattt atttttttta gcaaaaagat tgttaaaaaa ttaacagcgt tttttggcaa
     6901 aattattttt aaagaggaac aaattccccg tacatacggg gttttgtgtg taaaactgaa
     6961 aggagaattc aaaatggcaa agaaagtagt tttagcaggc gcatgccgta ccgctatcgg
     7021 caagatgggt ggagcactga gcaatactcc ggcagcagag ctgggatcaa tcgtaatcaa
     7081 agaggcattg aacagagctg gcgtgaagcc ggagcaggta gatgaagtat tgatgggatg
     7141 tgttatccag gcagcacagg gacagaacgt tgcacgtcag gcttccatca aggcaggact
     7201 tccgatcgaa gtaccggcag ttactctgaa cgttgtatgt ggttccggac tgaaatgtgt
     7261 caatgaagca gctactatga ttctggctgg tcaggcagat atcgttgttg caggtggtat
     7321 ggagaacatg tccatggctc cttatgccat gacgaaggct cgttttggat atcgtatgaa
     7381 caatgcgacc atcattgata ccatggtaaa cgatgcactt actgatgcat tcaaccacta
     7441 tcacatgatg atcacagctg agaacgtatg tgagaagtat ggtcttacaa gagaagaact
     7501 cgatgagttc tctgcaaaca gccagcagaa gtgcgagaag gcaattgctg agggcaagtt
     7561 cgatgacgag atcgttccgg ttccggttaa ggttaagaaa gagaccgtta tgttcgcaaa
     7621 agatgaggga ccgcgtcctg gcacaacagc tgagtcactg tccaagttaa aatgctgctc
     7681 cggcaaagag ggcggactgg taaccgcagg taacgcttcc ggtattaacg atggtgcagc
     7741 tgcaatcgtt gttatgagcg aggagaaggc aaaagagctt ggcgttaagc cgatggcaac
     7801 atgggtagcc ggagcactcg gcggagtaga gcctgagatc atgggtgtcg gacctgttgc
     7861 atctaccaga aaggtactcg agagaacagg acttaccatc gatgatatgg atctgatcga
     7921 ggcaaacgag gcattcgcag cacagtctgt tgcagttgca agagatctga agtttgatat
     7981 gtccaaggta aacgtaaacg gcggtgcaat cgcacttggt cacccggtag gagcttccgg
     8041 atgccgtatc cttgttacct tattacatga gatgcagaag agagacgcaa agagaggtct
     8101 tgctactctg tgcatcggtg gtggtatggg atgctctacc atcgttgaga gagactaatg
     8161 cagttatgag aacttctgaa ccggcgccag cctgtgggcg gcaccggaga agttcttgct
     8221 ttgcgtaata ggaatctttg ttcctatttt attaaaaggc atagaatcag ttattagaat
     8281 atatatatga tatttcagga ggtactgaca tgaaagttgg agttattggt gcaggaacaa
     8341 tgggacaggg tattgcaaaa gcatttgccc aggtagacgg atatgaagta gcactctgcg
     8401 acatcaagca ggagtgggct gacggcggaa aagagaagat caagaaaggc tatgcaagac
     8461 ttgtcgagaa aggaaaaatg acacaggaag cagtagacgg cattctcgca aagattaccc
     8521 caggcttaaa ggaagacctt tgcaaggatt gtgacctcat cgtagaggcg gctttcgagg
     8581 atatgggcgt taagaagact acattccagg agctggataa gattgcaaag ccagagtgca
     8641 tttttgcatc caacacatcc agcctttcca tcacagagat cggcaacgga ttgacccgtc
     8701 caatggtagg tatgcatttc ttcaacccgg cagaccgtat gaagttaatc gaggttatcg
     8761 caggtgtgaa tacaccggaa gagaccgttg aggcgatcaa gaagatctct gtagagatcg
     8821 gcaagactcc ggtacaggta aatgaggcag caggcttcgt tgtaaaccgt atcttaatcc
     8881 cgatgatcaa tgaggcagct ttcattaaga tggaaggtgt atctgatatc gcaggcatcg
     8941 atgcagcaat gaaactcggt gcaaaccatc cgatgggacc gcttgagtta ggtgatttca
     9001 ttggtctgga tatctgcctt gcaatcatgg atgttctcta caaagagaca ggcgacagca
     9061 agtaccgcgc atgcccgtta atccgcaaga tggttcgcgg cggcaatctt ggcgcaaaga
     9121 gcggcaaggg attctatgta tacaatgctg accgtacaaa gaccccggtt gatgctcagt
     9181 aaaatataag gtaataatgg aggaaatgat atcatggatt tcactttaga caagaaacat
     9241 gaaatggcgc gctccctgtt cagagagttc gctgagaacg aggtaaagcc tctcgctcag
     9301 gaagtagatg agacagagga attcccggtt gagaacgtga agaagatgca gaagctcggc
     9361 ttcatgggca tcccggtacc gaaggagtac ggcggacagg gctgtgaccc gctcacctat
     9421 gtaatgtgcg tagaggagct gtccaaggta tgcgctacaa caggtgttat tgtttctgca
     9481 catacttctc tctgcatcga tccgattatg acctacggta cagaggatca gaagcagaaa
     9541 tacgttaagc cgcttgcaac aggcgagaag ctcggtgcat tcgcactgac tgagccgggt
     9601 gcaggaaccg atgcgcaggg cgcacagacc aaggctgtat tagacggaga cgagtgggta
     9661 ttaaacggct ctaagtgctt catcacaaat ggtaaggttg cagatgttta catcgtaatc
     9721 gcaatcacaa gcgtaacaga ggataagaga ggcagaaaga agaagaactt ctctgcattt
     9781 atcgtagata agggagctcc gggcttctcc ttcggaacca aggagaagaa gatgggtatc
     9841 cgcggatcat ctacatacga gctgatcttc gaggattgca gaatcccgaa ggagaacctg
     9901 cttggcgtag agggcaaggg attcccgatc gctatgcaca cactggatgg cggacgtatc
     9961 ggtattgctg ctcaggcact tggtatcgca gagggcgcat tagaccgcgc aatcgcatac
    10021 accaaggaga gaaagcagtt cggtcgtacg attgcccagc agcagaatac acagttcaag
    10081 ctggctgaca tggctgcaag aattgaggct gcacagcttc ttgtatacaa agctgctatg
    10141 gcaaaggcaa cacagaaggt atattccgta gaggccgcaa aggcaaaact gttcgcagca
    10201 gagactgcta tggctgttac cacagaggtt gtccagctgt ttggtggata cggttacatc
    10261 agagagtacg atgtagagcg tatgatgcgt gacgcaaaga tcacagagat ctacgaggga
    10321 accagtgagg ttcagagaat ggttatctct ggtgcattac tgaaatagtc gtaatccgtg
    10381 gcataatata aaaatataag gagtgaaaaa tacatgaata tcgtagtatg tattaaacag
    10441 gtaccagata ccaagggcgg cgttaagttc aacccggacg gtacattaga cagagctgca
    10501 atgcttacca tcatgaaccc ggatgacaaa gcaggtcttg aggcagcact tagattaaaa
    10561 gagcagtacg gcgcagaagt aaccgttctt accatgggtc ttccgaaggc agacgaggtg
    10621 cttcgtgagg caatggcaat gggcgcagac aaaggaatcc ttgtaacaga tcgtgtgtta
    10681 ggcggagctg atacatgggc tacctccacc acaattgcag gcgcactccg caatctggat
    10741 tacgatctga tcatcaccgg acgtcaggcg atcgatggtg ataccgctca ggtaggaccg
    10801 cagatcgcag agcatctggg acttccggtt atttcctacg cacaggacat caagatcgat
    10861 ggagactatg ttgttgttca gcgtcagtac gaggacagat accatgagtt aaaggcaaag
    10921 atgccgtgcc tgatcacagc actttccgaa ctgaatcagc cgcgttacat gactccgggc
    10981 ggaatcttcg acgcatatga taaggaagtt accgtatggg gaagagcaga tcttaaggat
    11041 gtagatgatt ctgatcttgg attaaaggga tcaccgacaa agatcgcaaa ggcatctgac
    11101 aaggtacgta agggcgcagg cgagaaggtt gtccttgatc cggatgagtc cgtagcatac
    11161 ctgatcggca agttcaaaga gaagcacatt atctaacaaa tataaggaaa agtggtgaat
    11221 aaaatgggtt tagaagaata taaaggagta tttgtctttg cacagcaggt tgacaacgta
    11281 ttagacggcg ttgctttcga gttacttgga aaaggaaaag agctggcaaa ggatttaaac
    11341 acagaagtaa ctgcagtatt gattggctcc ggcgttaagg agctggcaga tcagcttgct
    11401 gagtacggcg ctgacagagt catcgtggta gacgatccgc agttaaaaga ctacagaaca
    11461 gagccgtacg cacatgcact ggcatctgtg atcaatgagt acaagccgga gatcatgctg
    11521 gttggcgcaa ctgcaatcgg ccgtgacctc ggacctactg tttccgctcg tgtgaagaca
    11581 ggtcttactg ctgactgtac gatgcttgag atcggcgatt tcccgctgaa cgcagcagct
    11641 ggacaggagc agcagcacaa tcagcttctt atgacacgtc ctgcatttgg tggtaacacg
    11701 atcgcaacga tcgcgtgccc gtacaaccgt ccgcagatgg ctactgtacg tccgggtgtt
    11761 atgcagaaga tcgctccgat cgcaggcgct aaggcaaatg tggtagagta caatccggga
    11821 ttcaccccga atgacagata tgtagagatc ttaaacattg taaaggctgt taagaataca
    11881 gcgaacatca tggatgcaaa gatcctggta tccggtggac gtggtgttgg ttccaaggag
    11941 aacttcaagc ttctcgagga tctggcagag gtacttggcg gtaccgtaag ctgctcccgt
    12001 gccgtagtgg agaacggctg gctgccggta gatttacagg taggacagac aggtaagaca
    12061 gtacgtccgc agatctactt cgcaatcggt atttcaggag ctatccagca cgttgcaggt
    12121 atggaggatt ctgatctcat cgttgctatc aacaaggatg aggatgcgcc gatcttcgac
    12181 gttgcagatt atggtctggt aggagatctc aacaagatcg ttccggcact gaccactgcg
    12241 ttaaaggcag aacttggcaa ataagcaaag aaaagtacat aggtgaaaag aactcccgcg
    12301 tggcagatgt ggcgtctgtc atgcgggagt ttttatgttt ttatttcacg agcttgacag
    12361 ttgccgtttt gcgtattatt gtcattccga aggagtgaaa gaacgtgaga cagacagtaa
    12421 cagaaaatcg attattcagt aaaaaagatt tgcggaaact gattattccg ctgatcttag
    12481 aacagacgct tgccatcacc gtgggaatgg cagatacgat gatgatttct tctgcgggag
    12541 aagcggcagt ttccggcgtg tcgctggtag acatgtttaa caacttaatc atcagtgtgc
    12601 tggcggcgct tgcgaccggc ggtgcggtgg taacgtcaca gtgtatcggt gcgggaagga
    12661 gagaggaagc ctgtcggtct gccagacagc ttgtgtttac agaggcggcg atcacgatcg
    12721 gtatcagtgt actggtactt ctttttcaca ggcagatcct cggattattc ttcggacaga
    12781 ttgaggcgga tgttatgcag aatgcgatca tctatctcat tatctccgtg ttctcatttc
    12841 cgcttctcgc agtgtatgac tcgtgtgcgg cgctgtaccg ttcgatgggg aatgcgcaga
    12901 tcacattgaa aatatcgctg ctgatgaatg tcatcaacgt tgtgggaaat gcaatcggcg
    12961 tgtatgtgct gaaactggga gtagcgggag tcgccattcc gtcgttggtt tcccgcggtg
    13021 tggcaggagt ggtgcttttt acgcttttgc ataatccgga caatctggtc tttctcacca
    13081 gaggaaaatt taaagtggac gcaacgattg tgaagcggat tctttttatc ggcatcccga
    13141 gcggaattga aaacggtatc ttccagctgg gacgtgtgct tgtggtcagc atcatagctg
    13201 cgttcggcac cagccagatc gcggcgaacg gggtggcgaa cagtctggat tccatgggat
    13261 gtattgtggg acaggcgatg agccttgcga tgattactgt gatcggacgc tgtgtgggag
    13321 ccggagaaga aggacaggtg cgctattaca ccaaaaagct gctgggagag acctattttt
    13381 atacggcggt catcaacagc atcattttac ttctgctgcc gtggatttta cagatctatg
    13441 gactgggaga ggagacgacg cgcctgtcct acattctggt catgattcat gatggaatgg
    13501 cgatcttcct gtggccggcg tcgtttgtgc tgccgaatat gctccgcgcc tgcaacgatg
    13561 taaaatacac gatggtcgtg tcgatttttt ccatgattac cttccgtatc ggcttcagtt
    13621 atgtgtttgg tgttcacatg ggatggatgg cagtcggggt gtgggctgcc atggtaattg
    13681 actgggtgtt ccgcgtactc tgttttgtgg gaagatatct ggcgggaacc tggcggaaaa
    13741 aatgtggtct tgtggtgcca actgcttgat aaatatttca catagtgata tactgcaaaa
    13801 gatgcaaaaa gttcctggca gacaggaacc ataaacaaag tatacagaaa agaggaaaag
    13861 actatgacga agtcgaaatt ttctacgaag tatctcgtgg agatggcgct tttggttgcc
    13921 atcatcttac tcatggcttt taccccgatt ggttacataa agacagcagg tcttgagatt
    13981 accctgattg tcataccagt tgcggtagga gctgtaacat tgggaccggc ggcaggagca
    14041 atcttaggcg gtgtgttcgg tatcacgagt tttatccagt gttttggaat gagcgcgttt
    14101 ggtacgctcc ttctgggcgt aaacccggta ttcacatttc tggtatgtgt gccgacacgt
    14161 attctggacg gatggctgac cggactgatc tatgcgggac tgaagaagac gaaaatacct
    14221 tccgcaggtt cggtaacgct tgccagcctt tgctgtccgt tgctgaacac cacatttttt
    14281 atgggaacac tggtaaccct gttcgcagga acaatgcgtg agcagttcgg catgacgaaa
    14341 gtgattccgt ttatcgcagc atttgtcggc gtgaacggtg ttgtcgaggc gatcgtatgt
    14401 ttcgttatcg gaacagcggt ttccatggca ttgaaaaaag cactccgcta ctaggaataa
    14461 taaataatat tatgagaaga aatacacttg gggatttcat gtggaaatgc agctggaaat
    14521 tcggttcaaa aaatgtggat ttctaaccag ccgaatgttt gcatctttga tgatccgtgt
    14581 ctttccaagt ttggagtact ccgcgggtca tgtctgcatc ccggaagaca atttgtagga
    14641 cttttgtgtg tgtcccttat tggcaccaga caacatatgg cataaatgcc acctttatgt
    14701 cgaacaaaac ataaccgcgc gaagccgatg tttgagacgg catctgcctt attttcaata
    14761 gcagatgccg gcgagtttcg gttcgcgcgg tt
//
