LOCUS       c00089_gnlPro..        10625 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NCNKIAFH_89.
ACCESSION   c00089_gnlPro..
VERSION     c00089_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:57:16
            Original ID  :: gnl|Prokka|NCNKIAFH_89
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10625
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10625
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2825:8056]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2826..8056
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..10625
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..10625
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             3..131
                     /locus_tag="ctg89_1"
                     /transl_table=11
                     /translation="QTGKMEIIVTFLAILELMKTGKIFISQEHTFDDILIQSKIVA"
     CDS             148..717
                     /locus_tag="ctg89_2"
                     /transl_table=11
                     /translation="MNEKNYEAIIEAILFTMGESVELDKIAAAIELDKKETKAIIEKMM
                     KHYENPSIGVKIIELDDAYQMCTKSEMYEYLIKIARQPKRHVLTDVLLETLSIIAYKQP
                     ITKVEIEKIRGVSCDHAVNKLVEYNLVCELGRLDAPGRPLLFGTTEEFLRSFGVQSIDE
                     LPVLNPVQVEEFKQEAEAEMHMKLDI"
     CDS             complement(646..909)
                     /locus_tag="ctg89_3"
                     /transl_table=11
                     /translation="MPAAVWKKYVNFASKNLPPSVYSGTFFVLWSSDNITYYPKRSMLI
                     AAIPYYPSLTDSHQDFFYVLYIKLHMHLGFRLLLKFLHLHRI"
     CDS             1432..2640
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S11"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_4"
                     /sec_met_domain="Peptidase_S11 (E-value: 9.1e-51, bitscore:
                     162.2, seeds: 11, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYRRFLSLLLSMSLLIFSPVRVRAQDVPAESELYAKSAVLMDADS
                     GRILYEKNGHEAMANASTTKILTCIVALENCDPASVVTVSKNAASQPKVHLGMHEGQQF
                     YLRDLLYGLMLESYNDCAVAIAEFAAGDVQGFAALMNAKAEKLGCEDSYFITPNGLDAK
                     DDAGFHHTTAADLAKIMKYCIKESLKSDEFLGITRTAQYSFSDAEGKCTYQCTNHNAFL
                     QMMEGALSGKTGFTGTAGYCYVGALQREDKTLIVALLACGWPNNKTYKWSDTRELMNYG
                     LEQFKKINLLQIEPDEAELAPIEVREGQGGHIGETVYLKPVVRNYAGEESILAPLSSEV
                     TLKYEIADRLYAPVKSGDYIGKIRYMLGEETLAERCVVAGETIGKIDYPWCLGQVLRLL
                     WIE"
     CDS             2826..4262
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_5"
                     /sec_met_domain="CT_Vparvula (E-value: 3.2e-125, bitscore:
                     408.2, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSSNDSLAMQRITRLVDENSFIEIGSLVTARSTDFNLAATDTPS
                     DGVITGHGLIDGNLVFIYSQDASVLNGTIGEMHAKKIAAVYDMAMKMGAPVIGFIDCAG
                     VRLQESVDALDGFGLIYAKEVAASGVVPQISAVFGSCGGGLAVVPALSDFAFIEESKGK
                     MFVNSPNAIDGNRIEKCDTSSAAFQADENGCIDATGTEDEILADIRELVGMLPLNNEGD
                     VYTDECADDLNRACESMDAMKGDPRYLLSEISDSHTFFETKAGYARDMVTGFIKLNGMT
                     VGAVANCTTVYDEEGKESEKFDNVLSAKGCEKAAEFVSFCDAFEIPVLTLTNVKGYKAC
                     KCSEKRLAKALAHLTSAFAGATCPKVNLITGEAYGTAYVAMNSKSIGADFVYAWPDAKV
                     GMMDADLAVKIMYADASADELTEKAKEYDALQGSVMTAARRGYVDLIVDPADTRKYLVD
                     AFELLYTKCAYTPDKKHGTK"
     CDS             4249..5001
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_6"
                     /sec_met_domain="OAD_gamma (E-value: 4.9e-26, bitscore:
                     81.0, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQNRKVKFMKKKLSLILCLCFMALGLAACGTDPTTVDYFGMTYD
                     ELQSSMEQEVSALVGLTDDNRTYIQSYGSDTAMKLVESWDEATSDVGAYQGLGEFNITK
                     AQKTLTVDQYVEYQNREVIVSYIYTYNYETEQPELTDASADKVYTLGEKMEKAALNTLM
                     GMGTVFVVLILISLIIYCFRFISVIQNKAAGKGKKNEAEAGAVVEQIGQREEQLTDDLE
                     LVAVISAAVAASTGASADSFVVRSIRRR"
     CDS             5043..5408
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 61.7; E-value:
                     1.5e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_7"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.8e-20, bitscore:
                     61.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYTITVNGNVYDVTVEENGAVSAPAAAPRRAAAPAAAPAAKPA
                     AAAGAGSVKIEAGAAGKVFKLEKSVGDAVKKGDSIMVLEIMKMETPVVATQDGTVASIN
                     VSVGDMVEAGALLATLN"
     CDS             5424..6572
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_8"
                     /sec_met_domain="OAD_beta (E-value: 2.7e-157, bitscore:
                     512.8, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYFSETMGNLLHQTAFFNLTWGNFVMIIVACFFLYLAIAKGFEP
                     LLLVPIAFGMLLVNIYPDIFADPEQMSNGVGGLFHYFYVLDEWSILPSLIFLGVGAMTD
                     FGPLIANPISFLLGAAAQFGIFGAYFLAIMMGFNDKAAAAVSIIGGADGPTSIFLAGKL
                     GQSGLMGPIAVAAYSYMALVPIIQPPIMKLFTTKKERAIKMENLRPVSKLEKILFPIVV
                     TVVVCTILPTTAPLVGMLMLGNLFRESGVVRQLTDTASNALMYIVVILLGTSVGASTSA
                     EAFLNTTTLKIVALGLIAFMFGTAAGVLFGKLLCFLTKGKINPLIGSAGVSAVPMAARV
                     SQKVGAEEDPTNFLLMHAMGPNVAGVIGTAVAAGVFMAIFGI"
     CDS             6638..8056
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_9"
                     /sec_met_domain="HMGL-like (E-value: 9.3e-46, bitscore:
                     146.1, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 1.2e-59, bitscore:
                     191.1, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGY
                     NAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVE
                     YFVQKSIANGIDIIRIFDCLNDVRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDYWME
                     MAKKVEDMGADSLCIKDMAGLLVPTKATELIQALKSATNLPIELHTHYTSGVASMTYMK
                     AVEAGCDIIDTAMSPFSMGTSQPATEVMVEAFKGTEFDTGLDQNLLAEIADYFRPMREE
                     ALESGLMNPKVLGVNIKTLLYQVPGGMLSNLLSQLKEQGKEDKFEEVLAEVPRVRKDLG
                     EPPLVTPSSQIVGTQAVFNVLMGERYKVATKETKDVLLGKYGQTVKPFNPEIVDKVLGE
                     EKKNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALFPQVATEFFKYREAQQKKV
                     DASVADTKNGAYPV"
     CDS             complement(8120..8602)
                     /locus_tag="ctg89_10"
                     /transl_table=11
                     /translation="MRDITPATNFIHTLMQSPPDASAWIAGGNNFPSISGLARFYATEY
                     EGTLVEVEVFGLPNVATPGSSNFYGMHIHENGVCVSPFTSAGAHYSRTPAEHPNHSGDM
                     PPLLGNQGYAWMAFYDKRFTIPEIIGKSLIIHRMPDNFTTQPAGDSGERIACGVIK"
     CDS             8764..9696
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SIS"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_11"
                     /sec_met_domain="SIS (E-value: 1.4e-24, bitscore: 76.1,
                     seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTNDLSNRINDAYSKLSKGQKRLATYITDNYDKAVFLTAAKLG
                     EVVGVSESTVVRFATHLGYKGYPEFQSALEELVRNKLNSIQRMEVTYGRISQSKILETV
                     LKSDADKIHSTLEKIDQNAFELAVDTILHAKHIYIIGIRSCAPLASFMAFYFTLMFPNV
                     HLIQTSSSSEIFEQMVRIGKEDVIIGISFPRYSMRTLKAMEFANNRSAKVITLTDSVHS
                     PMNLYSSCNLIADSDMASIVDSLVAPLSVINALIVALCMKKQNEVAKTLEMLEDIWDEY
                     QVYENDEINYIDDSIKMRYGKVGDAELYE"
     CDS             9689..10624
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HI0933_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 33.6; E-value:
                     7.7e-11)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_12"
                     /sec_met_domain="HI0933_like (E-value: 1.6e-96, bitscore:
                     313.1, seeds: 46, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKVIVVGGGPAGMFAAIAATERGHEVVLLEKNEKLGKKLYITGK
                     GRCNITNAGDMDTLFANVMTNPKFLYSAFYDYDNQRVIDFFEENGLATKVERGNRVFPV
                     SDHSSDVIATLQRVLKRTGVMVQLHAEVRQILTGEGAVRGVKLADGSVLEAERVILATG
                     GFSYQTTGSTGDGYRFARELGHTVTQITPSLVPFYVKETCAARMQGLALKNVQVRIRDG
                     KKLLYDEFGEMLFTHFGVSGPLILSASAAIKPGRVEKELAMEIDLKPALTEEQLDARLL
                     REFEEAKNKQFKNAVVHLFPAKMIPVMLEAG"
ORIGIN
        1 cacagacggg caagatggag atcattgtca cgtttctcgc cattctggaa ctgatgaaga
       61 caggcaagat atttatatca caggaacaca cctttgatga tattctgatt cagtcaaaga
      121 ttgtggcata gagttggagt tttcgggatg aatgaaaaga attacgaggc gatcattgag
      181 gcgatcctct ttacgatggg agaatcggta gagctggaca agatcgccgc agccatagaa
      241 ctggataaaa aagagacaaa agcgatcatt gagaagatga tgaagcacta cgagaatccg
      301 tccatcggtg tgaagattat tgagctggac gatgcgtatc agatgtgcac caaaagcgag
      361 atgtacgaat atctgatcaa gattgcgaga cagccaaagc gccatgtcct gacggatgtt
      421 cttctggaga cgctgtccat catcgcctat aagcagccga tcaccaaggt ggagatcgag
      481 aagatccgcg gtgtttcctg cgatcacgcg gtgaacaagc tggtggaata caatctggta
      541 tgcgagcttg gcagactgga tgcaccgggg cgcccgctgc tcttcggaac gacggaagag
      601 ttcctacgaa gctttggggt acagtcaatc gacgaactcc cggtattaaa tccggtgcag
      661 gtggaggaat ttaagcagga ggcggaagcc gagatgcata tgaagcttga tatatagaac
      721 ataaaagaaa tcctggtgcg aatctgtgag agacggataa taaggaattg ccgcaataag
      781 catagaccgc ttggggtaat aagtaatatt atcagaagac cagagaacaa aaaaagttcc
      841 agaataaaca gacggcggca aattctttga agcaaaattc acgtatttct tccagaccgc
      901 agctggcatc ccattccgtg tctttgccac gtttggacac tcacggagtg tgtccgacaa
      961 gccgcagaca atgagcggac tttctggtgt ccctaatggc acctcttcac agacactata
     1021 aaaccgcgcg aaccgaaact cgccggcatc tgctattgaa aataaggcag atgccgactc
     1081 agacatcggc ttcgcgcggt tatgttttgt tcagaggtgt cataagggac acacacaaaa
     1141 gtcctaaaat tgtctgccgg cttgcggaca cagtccgtgg agtgctccaa acttggaaag
     1201 acacggatca tcacggatgc cagctgcggc atggttagaa ataccggatt ttgcggtcag
     1261 aatttgccgc cgcatgttta tctggaactt ttaagtatct atttcttata atattactta
     1321 tcacccgacg agttctatgt ttattgcggc aattccttat tccgtgccgt ttctgttctg
     1381 caaaaaatga gggacgcata tacatcagtg aagtcatttt gggagaatgg catgtatcga
     1441 cgctttcttt ctctattgtt aagtatgagt ttacttattt tttcgcctgt gcgtgtccgg
     1501 gcgcaggatg tgccggcaga gtcggaactg tacgcaaaat ctgccgtttt gatggatgcg
     1561 gattccggaa gaattctcta tgagaaaaac gggcatgaag cgatggcgaa tgcgagtacg
     1621 acaaagattc taacctgtat cgtggcactg gaaaactgtg atccggcgag cgtggttacg
     1681 gtgtcaaaaa atgcagcgtc acagccgaag gtacatctgg ggatgcacga gggacaacag
     1741 ttttatctga gggatctttt atatggcctg atgctggaat cctacaatga ctgtgcggtg
     1801 gccatcgcgg aatttgcggc gggggatgtg cagggatttg ccgcgctgat gaatgcgaag
     1861 gcagagaaac tcggctgtga ggacagctac tttattacac cgaacggact ggatgcgaag
     1921 gatgatgccg ggtttcatca cacgacggcg gcagaccttg ctaagattat gaaatattgt
     1981 attaaggaat cgttaaaaag cgacgaattt ctcgggatta cacgaacggc gcagtattct
     2041 ttttctgatg cagaggggaa atgtacgtac cagtgcacga accacaatgc atttttgcag
     2101 atgatggaag gggcgctttc cgggaaaacg ggatttaccg gaaccgccgg atactgctat
     2161 gtcggagcgc tgcagcggga ggacaaaacg ctgatcgtgg cgctgctcgc ctgtggatgg
     2221 ccgaacaaca agacctataa gtggtccgac accagagaac tcatgaatta cggattggag
     2281 cagttcaaaa agatcaatct gctgcagatt gagccggacg aagcggagct tgcgcccata
     2341 gaggtgcggg aaggacaggg cggacatatc ggcgaaacgg tgtatttaaa accggttgtc
     2401 aggaactatg caggggagga gagtatcctg gcgccgctaa gcagtgaggt tacgcttaag
     2461 tatgagattg cagaccggct gtatgcaccg gtgaagagcg gagattacat cgggaagatt
     2521 cgttatatgc tgggggagga gacgctcgcg gagcgatgtg tggtagccgg ggagacaatc
     2581 ggaaagatcg attatccgtg gtgtctgggg caggtgcttc ggcttctctg gatcgaataa
     2641 aaaacacaaa caaaaagcgt caaaaaatta acaatttact tgaaatattg cagaaaagca
     2701 attataattt aacatagcgt aatttgttga atttttatac aaagacgcat ggatttacat
     2761 cattcactat gaagccgcat cccaaatgga tgtggacaac aatttataga tggagggcaa
     2821 aaagaatgaa tagtagcaat gatagcctgg ctatgcagcg catcaccagg ttagtcgacg
     2881 agaacagttt cattgagatc ggttctctcg taaccgcaag aagcacagat tttaatcttg
     2941 cagcaacgga tactccttcc gacggagtca tcacaggaca cggcctgatt gacggcaatc
     3001 tggtgttcat ttacagccag gacgcttctg ttttaaacgg aacaattggc gagatgcatg
     3061 cgaagaagat cgctgcagtg tatgacatgg cgatgaagat gggagcaccg gtgatcggct
     3121 ttatcgattg cgcgggagtc cgtttacagg agtctgtaga tgcgcttgac ggcttcggac
     3181 tgatctatgc aaaagaagtt gcagcatccg gcgtggttcc gcagatttca gccgtattcg
     3241 gcagctgcgg cggtggactg gcagtggtac cggcactttc tgattttgct tttatcgaag
     3301 aatccaaagg aaagatgttt gtgaattctc cgaacgcaat tgacggaaac cgcatcgaga
     3361 agtgcgacac atcatctgcg gcattccagg cagatgagaa cggctgcatc gacgcgacgg
     3421 gaacagagga tgagatcctt gcagacatcc gtgagctggt cggcatgctt ccgctcaaca
     3481 acgagggaga tgtctacaca gacgagtgtg cggatgacct gaaccgtgca tgcgagagca
     3541 tggatgccat gaagggtgat ccgagatatc ttctcagtga gatttcggac agccatacat
     3601 tcttcgagac aaaggcaggc tatgccagag atatggtgac cggatttatc aagttaaacg
     3661 gtatgacggt cggcgcagtt gcaaactgca caacggtata cgacgaagag ggcaaagaga
     3721 gcgagaagtt tgacaatgtt ctttccgcaa agggatgtga gaaggcagca gagttcgtat
     3781 ctttctgcga cgcattcgag atcccggtac tgactctgac gaatgtcaag ggctacaagg
     3841 catgcaagtg ctctgaaaag cgtcttgcaa aggcacttgc tcatctgacc tccgcatttg
     3901 cgggagctac atgtccgaag gtgaacctga tcacgggaga ggcatacggt accgcttatg
     3961 ttgctatgaa ttccaagtct atcggtgcgg atttcgtata tgcatggccg gatgcaaagg
     4021 tcggcatgat ggatgcggat cttgcagtaa agatcatgta cgcggatgca tctgcggatg
     4081 agcttacaga gaaggcaaag gaatatgatg cactgcaggg cagcgttatg acagcagcca
     4141 gaagaggcta tgtggatctg atcgttgatc cggcagatac gagaaagtat ctggtagatg
     4201 cgtttgaact gttatacaca aagtgtgcat atacaccgga caagaaacat ggaacaaaat
     4261 agaaaggtga agtttatgaa gaaaaaatta tctcttatac tttgtctttg ctttatggct
     4321 ctgggacttg cagcatgcgg aacagatccg acaacggttg attatttcgg aatgacttac
     4381 gatgaactgc agagcagcat ggaacaggaa gtttccgcgc tggttggtct tacggacgac
     4441 aacagaacct atatccagtc ctacggcagc gatacggcaa tgaaacttgt cgagagttgg
     4501 gatgaggcga caagtgatgt cggcgcatat cagggacttg gagaattcaa tatcacaaag
     4561 gcacagaaaa ccttaacagt agatcagtat gtagagtatc agaatcgtga agtcatcgtt
     4621 tcttacatct atacatacaa ttacgagacc gagcagccgg agcttactga tgcatccgcg
     4681 gacaaggtat atacgctggg cgagaagatg gagaaggctg cgttaaatac cctgatggga
     4741 atgggaaccg tattcgtggt tctgatcctg atcagcctta ttatttactg tttccgcttt
     4801 atctctgtaa tacagaacaa ggcggccggc aagggcaaga agaacgaggc agaggcaggc
     4861 gctgtggtag agcagatcgg acagcgtgaa gagcagctta ccgatgatct tgaacttgtg
     4921 gcagttattt ccgcagcggt tgcggcaagc accggtgcat ccgcggattc tttcgttgtg
     4981 agatcgatcc gcagacgcta attcgtataa atgaaaagaa atcatattag gaggatattt
     5041 caatgaaaag ctatacaatt acagtaaacg gcaatgtata tgatgtaacc gtagaagaga
     5101 acggcgcagt gagcgcaccg gcagcagcac cgagacgtgc agcagcaccg gcggcagcac
     5161 ctgcagcaaa gccggctgct gcagcaggcg caggcagcgt taagatcgag gcaggcgcag
     5221 caggaaaggt attcaaactt gagaagtctg tcggtgatgc cgttaagaag ggagacagca
     5281 tcatggttct tgagatcatg aagatggaga ctccggtcgt tgcaacgcag gacggtacgg
     5341 tagcaagtat caacgtaagt gttggagata tggtagaagc gggcgcatta cttgcaacat
     5401 taaactaatt taggaggaag acaatgagct attttagtga gacaatgggg aacctcctgc
     5461 atcagaccgc tttctttaac cttacctggg gaaattttgt gatgatcatt gtcgcatgtt
     5521 ttttcctgta tctggctatc gcgaaggggt ttgagccatt gcttcttgtt ccgatcgcat
     5581 tcggtatgct gcttgtaaat atctatccgg atattttcgc agacccggag cagatgagca
     5641 atggtgtagg tggtttattc cactactttt atgtgttaga cgagtggtca atcttaccgt
     5701 ctctgatttt ccttggagtc ggtgctatga cagatttcgg accgctgatt gcaaacccga
     5761 tcagtttcct gcttggagct gcagctcagt tcggtatttt cggagcgtac ttccttgcaa
     5821 tcatgatggg ctttaatgat aaggcagcag ctgctgtttc catcatcggt ggagccgacg
     5881 gaccgacctc cattttcctt gccggaaagt taggacagtc gggactgatg ggaccgatcg
     5941 ctgtggcggc atattcctat atggcgcttg tgccgatcat ccagccgcca attatgaagc
     6001 tgtttacaac caaaaaagag cgtgccatta agatggagaa cttaagaccg gtttccaagc
     6061 tggagaagat tctcttcccg atcgtggtta cggtggttgt atgtacgatt cttccgacga
     6121 cagcgccgct ggttggtatg ctgatgctcg gtaacctgtt ccgcgagagc ggtgtggtcc
     6181 gccagctgac cgataccgca tccaacgcat tgatgtacat cgtagttatc ctgctcggta
     6241 cttccgtagg tgcatctacg agtgcggagg cattcttaaa tacaacgaca ttaaagatcg
     6301 ttgcacttgg actgattgca tttatgttcg gtacagcagc cggtgtcctg tttggtaagc
     6361 tgttatgctt cctcacaaag ggcaagatca atcctctgat cggatctgcc ggtgtatctg
     6421 ccgttcctat ggcagcccgt gtatcccaga aagtcggtgc agaggaagat ccgacgaact
     6481 tcctgctgat gcatgcgatg ggaccgaacg ttgccggcgt tatcggtact gcagtagcag
     6541 ccggtgtatt catggcaatt ttcggaattt agtgaaagag caaatgccgt catagacgga
     6601 tagcggtatc aaagtatccg ctgataagga ggaaaaaatg tcagaagttg ttaaaaaacc
     6661 tttaaaaata acagagacgg tgctgcgtga tgcgcaccag tccctgattg cgacaagaat
     6721 gacaaccgag cagatgcttc cgatcgtaga caagatggat aaggtcggat acaatgcggt
     6781 tgagtgctgg ggcggtgcta ccttcgatgc atgccttcgt ttcctgaaag aagatccgtg
     6841 ggatcgtctc cgcaagttaa gagacggatt taagaacacc aagcttcaga tgctgtttcg
     6901 tggacagaac atcttaggat atcgtccgta tgcggatgat gtggtagagt atttcgtaca
     6961 gaaatcgatc gcaaacggta ttgatatcat tcgtattttc gactgcttaa acgatgtccg
     7021 caacctgcag acagccgtta ccgcatgtaa caaagagaaa ggacatgcac aggtcgcatt
     7081 atcctataca cttggcgatg cctacactct ggattactgg atggagatgg cgaagaaggt
     7141 agaggatatg ggtgctgatt ccttatgtat caaggatatg gcaggtcttt tggttccgac
     7201 gaaggcaacc gagctgattc aggcattgaa gtctgcaacc aaccttccga tcgagctcca
     7261 tacacactat acttccggtg tcgcttccat gacatacatg aaggcggttg aggcaggttg
     7321 tgacattatc gatacagcaa tgtctccgtt ctccatggga accagccagc cggcaaccga
     7381 ggttatggta gaggcattca agggcacaga gttcgatacc ggtctggatc agaacctgtt
     7441 agctgagatc gctgactact tccgtccgat gcgtgaggaa gcgttagaga gcggtctgat
     7501 gaacccgaag gttctcggcg ttaacatcaa gactctgtta tatcaggtac cgggtggtat
     7561 gttatccaac cttctgtccc agttaaaaga gcagggcaag gaagacaagt tcgaggaggt
     7621 tcttgcagag gttccgcgtg tgcgtaaaga cctcggtgag ccgccacttg ttacaccgtc
     7681 ctcacagatc gttggtacac aggctgtgtt caacgtactg atgggagagc gctacaaagt
     7741 ggcaaccaag gagaccaaag acgtactgct tggaaagtac ggtcagactg taaaaccgtt
     7801 caacccggag atcgtagaca aggtacttgg cgaggagaag aagaacgcca ttacctgccg
     7861 tccggcagat ctgcttgagc cggagctgga taagatcgag gctgagatga agcagtggaa
     7921 gcagcaggat gaggatgtac tgtcctacgc actgttcccg caggttgcaa ccgaattctt
     7981 caagtaccgt gaggcacagc agaagaaggt cgatgcaagc gtcgcagata ctaagaacgg
     8041 agcatatccg gtataatcaa aaaacagtta taacaaaaag cgggtgagca gtcacctgct
     8101 ttttttgctg ccttttttgc tattttatca caccgcaggc aatccgttcg ccggagtcgc
     8161 ccgcgggctg cgttgtaaag ttatccggca tccggtgaat gatgagggat tttccgataa
     8221 tttccggaat cgtaaaacgc ttgtcataaa atgccatcca ggcataaccc tggttgccca
     8281 gaagcggagg catgtcgccg ctgtgattcg ggtgttcggc cggcgtacgg ctgtaatggg
     8341 caccggcact ggtaaacggg gagacgcaca cgccgttttc gtggatgtgc atgccgtaaa
     8401 aattactgga gccgggagtc gcgacgttcg gaagaccgaa gacttcaacc tccacgagag
     8461 ttccttcgta ttctgtggca tagaaccggg caagcccgga gatggaagga aaattgttgc
     8521 cgccggcaat ccaggcggat gcatccggcg gagactgcat cagtgtatga ataaaattgg
     8581 ttgctggtgt gatatcgcgc atagctgcct caatggaaga gtgtcattgg tttattatat
     8641 gcagcggcgg aaaaatgcgc tgcatgtgag gaaagttcca tgcataattt cttgcaaaac
     8701 acaatgtatt tgatataata aaaacattgg gcggatcaca attccgcaga agtgaggaga
     8761 agcatgagca atacaaatga cctgagtaac cgcatcaatg atgcttacag taagctgagc
     8821 aagggacaga aacggctggc aacctacatt acggacaatt atgacaaggc ggtatttctg
     8881 accgctgcca agctgggcga ggttgtgggc gtgagcgagt ccacggtggt gcgctttgca
     8941 acgcatctgg gatataaggg gtatccggaa ttccagagtg cgctggagga actggtgcgc
     9001 aataagctga actccattca gcgcatggag gttacttacg gcaggatcag ccagtcgaag
     9061 attctggaga cggtgctgaa atccgatgcg gacaagattc attccacact ggaaaagatt
     9121 gatcagaatg catttgaact cgcggttgac acgatcctgc acgccaaaca tatctatatc
     9181 atcggaatcc ggagctgtgc gccgcttgcc agcttcatgg ctttttattt taccctgatg
     9241 tttccgaacg ttcatctgat tcagacaagc agttcgagcg agatttttga acagatggtg
     9301 cgcatcggca aggaggatgt gatcatcgga atcagttttc cgcgttattc catgcgtact
     9361 ttaaaggcga tggaatttgc gaataaccgg agtgcgaagg taatcacact caccgacagc
     9421 gtgcattccc cgatgaacct ctattcttcc tgcaatctga ttgcggacag cgatatggct
     9481 tccattgtgg attccctggt tgccccgctg tcggtgatca atgcgctgat tgtggcgctc
     9541 tgcatgaaaa agcagaacga ggtggcgaaa acgcttgaga tgttagagga tatctgggac
     9601 gaataccagg tttatgagaa cgatgagatc aattatatcg atgattccat caagatgcgg
     9661 tatggaaaag taggagatgc ggaactgtat gagtaaggtg atcgtagtcg gcggcgggcc
     9721 ggcgggaatg tttgctgcca ttgcggcgac ggagcgggga catgaggtcg ttttgctcga
     9781 gaaaaatgaa aagcttggga aaaagctcta catcaccggc aaagggcggt gcaacatcac
     9841 gaatgcgggg gatatggaca ccctgtttgc caatgtgatg acgaacccga agtttttgta
     9901 cagcgctttt tacgattatg acaatcagag agtgattgat ttttttgagg agaatggtct
     9961 ggcgacgaag gtggagcgcg gcaaccgtgt attcccggta tcggatcatt cttcggatgt
    10021 gatcgccacg ctgcagcggg tactaaagcg cacgggtgtt atggtgcagc ttcatgcgga
    10081 agtgcggcag atcctcaccg gggaaggcgc ggtgcgcggt gtgaagcttg ccgacggaag
    10141 cgttcttgaa gcagagcgcg tgattcttgc aaccggagga ttttcttatc agacgaccgg
    10201 ttcgaccgga gacggctatc gttttgcgag ggaactcgga catacggtga cacagattac
    10261 gccgtcactg gttccgttct acgtgaagga gacctgcgcg gcgaggatgc aggggctggc
    10321 gctgaaaaat gtgcaggtgc gcatccggga cggaaaaaag ctgttgtatg acgaattcgg
    10381 cgagatgctc tttacccatt tcggggtaag cgggccgctg attttaagcg ccagtgcggc
    10441 aatcaagccg ggccgcgtgg agaaggaact ggcgatggag atcgacttaa agccggcact
    10501 cacggaggaa cagctggatg cgagacttct gcgtgaattc gaggaggcaa agaataagca
    10561 gtttaaaaac gccgtcgtgc atctgttccc ggcgaagatg attccggtga tgttagaagc
    10621 cggag
//
