LOCUS       c00259_gnlPro..         5634 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CMKIGCCJ_259.
ACCESSION   c00259_gnlPro..
VERSION     c00259_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:09:50
            Original ID  :: gnl|Prokka|CMKIGCCJ_259
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5634
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5634
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2593]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2593
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5634
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5634
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..1807
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 167.3; E-value:
                     2e-51)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.6e-52, bitscore:
                     167.3, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.7e-39,
                     bitscore: 122.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDG
                     DSVYRLFYDTIKGGDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGG
                     AQVSRTFYARGQTGQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARN
                     LITGKIERYAAHAVVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHP
                     TCVPVKGDFQSKLTLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLER
                     RYPAFGNLVPRDVASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLF
                     DMYEEITNDNPYETPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLG
                     ASALMQGLADGYFVLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGK
                     ETVDTIHRKLGHIMWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELE
                     KALRVADFIEIGTLMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGE
                     DKDPVMLKEDLNYEFVVPQTRNYKK"
     CDS             1838..2593
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg259_2"
                     /sec_met_domain="Fer2_3 (E-value: 5.6e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.1e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2630..2740)
                     /locus_tag="ctg259_3"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             3016..4227
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg259_4"
                     /sec_met_domain="Radical_SAM (E-value: 3.2e-17, bitscore:
                     51.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPDRIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQIEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
     CDS             4261..5493
                     /locus_tag="ctg259_5"
                     /transl_table=11
                     /translation="MKILLVTRGSQGDVLPYLAIAAELERRGHEVTINLPQIFEETVKP
                     YGFKYVLQQFDDIGGMIDSAAQNSHKFRPFLKWMRNVIDKQFDQLIPLLKEHDILVSTN
                     SEFAVASIAEYCKKPLIRTAYAPFLPGKKIPPAVLPFPKPNPIITPAILWKLMNRMTNF
                     MVKDTINKNRAKYGLAPIRNFGYHAGERSYNYLLFSQHLGNIDPDWTFKWSIGGYCFND
                     TFQYDEKAYEEMISFVDSAQSPIIFFTLGSCSSKDGNRFSKALVDICKKHDYRLIIGSG
                     WAKTGITLQADKHLFLMKQPVPHNLIFRHCDGVIHHGGCGTTHSVGRAGKPQLITPLII
                     DQPYWSYRIHQLGLGPEGLKIAKASEQEIERKVCDLVTNPLYKKNAAHIGEMIQKEGGI
                     KNICDSIERFQ"
ORIGIN
        1 agcttccgct gccgcatctt tggcagagat gggattcaac gtattgaact tctgtatcca
       61 agattctccc cgccgtgcgc actctatcgc cgctcaaggt ggtatcaacg ccgctaagaa
      121 ctaccagaat gatggtgact ctgtttaccg tttgttctac gatacgatca aaggtggtga
      181 ctaccgtgct cgtgaggcta acgtttatcg tttggctgag gtttctaact caatcatcga
      241 ccagtgcgtg gctcaaggcg ttcctttcgc tcgcgaatat ggtggtttgt tggataaccg
      301 ttcattcggt ggcgctcagg tatcgcgtac gttctacgcc cgcggccaga cgggccagca
      361 gcttttgtta ggtgcttatt cagcgttgag ccgtcagatc ggtttgggta aggttaagat
      421 gtacactcgt catgagatgt tggatgtagt taaggtagac ggtcgtgctc gtggtatcat
      481 cgcccgcaac ttgattacag gtaagatcga gcgttacgcc gctcacgctg tagttgtagc
      541 tacgggtggt tacgttaata cgttcttctt gtcaaccaac gctatggcat ctaacggttc
      601 cgccgcatgg caatgctaca agaaaggcgc ttacttcgct aatccttgta tggttcagat
      661 ccacccgact tgcgtaccgg tgaaaggtga cttccaatct aaattgactt tgatgtccga
      721 atccttgcgt aacgacggcc gtatctgggt tccgaagaaa ctggaagacg ctaaagctct
      781 acaggccgga acgaagaaag gtaaagatat tcccgaggca gaccgtgatt attatttgga
      841 gcgtcgttat ccggcattcg gaaacttggt tcctcgcgac gtggcttctc gtgccgctaa
      901 agagcgttgc gacgcaggtt tcggcgtgaa cagcacgggc ttggccgtat tcttggattt
      961 ctccgacgcg atcaatcgct tgggtaaaga ggtcgttaag cagaaatacg gtaacttgtt
     1021 cgatatgtac gaagagatca cgaacgataa tccatacgag actccgatga tgatctaccc
     1081 ggcattgcac tactcaatgg gtggattgtg ggttgactat gagttgatga cctccattcc
     1141 gggtctgttc gctatcggtg aggcaaactt ctccgatcac ggagcgaacc gtttaggtgc
     1201 ttccgctttg atgcagggat tggccgatgg ttatttcgta ttaccataca cgatccagaa
     1261 ctatttgtcc gaccaaatcc aagttcctcg cttcagcacg gatctgccgg agttcgtcga
     1321 ggcagaaaag gccatcaagg atcgtatcca aaaattgatg aacgtcaagg gtaaagaaac
     1381 cgtagacact atccaccgca aactaggtca tatcatgtgg gaacatatcg gtatggcacg
     1441 cgacgcaaaa ggtttgcaag aagctatcca gatgctgaaa gacttgaaga aagagttctg
     1501 gagcaacgta ttcatcccag gcaacgcaga caacttgaat accgagttag agaaagcatt
     1561 acgtgtagcc gactttatcg agatcggtac attgatggca cacgatgctt tggaccgcgc
     1621 tgagtcttgt ggtggtcact tccgtacgga gcaccagaca gaagaaggtg aggccttgcg
     1681 tcatgacgat aagttcatgt acgtatcttg ctgggagtac caaggcgagg acaaagatcc
     1741 tgtcatgttg aaagaggatt tgaactatga gttcgttgtt cctcaaacac gtaactacaa
     1801 gaagtaataa tcatcaatct cagaaaaaat tagaatcatg gataaagata taagtgtaac
     1861 acttaaggtt tggcgtcaga gaggtccgaa ggagaaagga cagttcgaga catacaaaat
     1921 tgagaagatc aatcagagtg tctctttctt ggaaatgttg gatatcttga acgagcagtt
     1981 ggtcaacgag ggtaaggaac cgatcgtatt cgatcacgac tgccgcgagg gtatctgtgg
     2041 catgtgttca ttgtatgtaa acggacaccc gcacggaccg gcaactggag ctacgacttg
     2101 ccagttatat atgcgtcgtt tccacgatgg agagacgatc acgatcgaac cgtggcgttc
     2161 cgcaggtttc cctgttatcc gcgacttgat ggtagaccgc tacgcttacg acaaaatcat
     2221 ccaagcgggt ggtttcacat ctgtcaacac aggtggtgta cccgacgcta atgcgatacc
     2281 tatcccaaag aaggacgcag acttagccat ggacgctgcc gcttgtatcg gttgtggcgc
     2341 ttgtgctgcc gcttgtaaga atggttccgc tatgttattc gtatccgcta aggttagcca
     2401 gctggctttg ttgcctcaag gtaaagtcga ggctgctcgc cgtgccaaag ctatggtcgc
     2461 taaaatggat gagcttggct tcggtaactg tacgaacaca cgtgcttgcg agatggaatg
     2521 tccgaagaat atctccgtta gcaacatcgc tcgtttgaac cgcgagttca tttgcgctaa
     2581 attacaagac taatatttga ttagtatatc atggaaaagg cgtctttctc taaaagaaag
     2641 gcgccttttt gtttttgtta tatgtcgtaa aacataattt agcattataa cccttgacag
     2701 gaattgaaaa gtccgtatat ttgcatcgtg gttttttcat aatagtatta gatttaaggt
     2761 taacaaagtt tggttagggc ttgtcgtgag acaggcttta attgtttata gacctacctt
     2821 ttaaacatgg accataaaat actgcaaata gagattgttt agcgtcattt tatcgtcgat
     2881 ctttgccttt aaagatcata tagtatatgc taataagtat tttatttggc ttcttatgtt
     2941 attttctccg ggtgttgatc aaacacaagg aaaaaatcat gcgccagata agacaaatgg
     3001 taaaacaaaa ataaaatgga caaaaaacga accaccagtt ctttcagggc aatgagcacg
     3061 gaaagaaaac tcgagtcgat cttccttttc gtaacaggcc gatgcaacgc caaatgcgca
     3121 atgtgtttct atgcccaaga aatggataag aagcaacccg acctcacttt cgatgaaatc
     3181 aagaagatat cagaaaccgc cgggcaattc aatcgattat ggatatccgg gggtgagcct
     3241 accttgcgtg aggatctacc ggaaatattg gagttattct acaaaaacaa ccatatcaaa
     3301 gatgtgaaca tccctacgaa cggcttaaaa ccagaccgga tcgtcgaatg ggtaaaacgg
     3361 ttccgccaga actgcccgga ttgtaatatt aacgtaagcc tttccttgga tggtttcggt
     3421 acgacccacg atacacagag aggcgtaccg ggaaacttct ataaggcctt ggaaaccctg
     3481 cgtaagatac aggagaattt cggagatgac ggcaaagtct taaaaaatat agcaaccgtt
     3541 atcacgaaat acaatataga ccagatagaa gacttcatga cttgggtata cggacgtttc
     3601 cgtacctcca cgcatacgat tgaggctgcc agaggcatga cccgtgacga tggcgtgaaa
     3661 atcctaaccg agtcaacgct acgcaaaata caagatgaca tatcccctat ctattcagct
     3721 tatgcggatc ggatggtagc ggataccacc ggcttaagga aaccgatcac acgcttcttc
     3781 tatctcggcc ttatacgtac cttatataat gtgcgtgcca gcaacataga tcacccgacc
     3841 ccttggggga tggattgtac ggccggagag actaccttgg taatcgacta cgacggacgt
     3901 tttcgtgcct gcgagctacg ggaaccgctc ggaaatataa aagagtatgg ttgcgatata
     3961 agtaaagtga tgaatagcga agcgatgaaa caggaaatag ccgctatcgg acatgggtat
     4021 aaagccaact gttggtgtac gcacggctgc tggatcacct catccgtcat tttcaatcca
     4081 cgtaagatga ttcgctccgt atataaagga taccgggaaa cgaaacgatt gaatcatccc
     4141 ttagccatca atgagcagaa actacaaacg atggaagcca aataccatct ggacattgag
     4201 cgtttgcgtc aactgaatat ccggtaaaca agaccctcat agcccataaa taaacgtagc
     4261 atgaaaatac tattagtaac cagaggctca caaggcgacg tccttcctta tttggccata
     4321 gcggccgaat tggaaagacg tggccatgag gtgaccatca atctccctca aatattcgag
     4381 gagaccgtta aaccctacgg atttaaatac gtattgcagc agtttgacga tatcggcggc
     4441 atgatcgata gcgcagcgca aaactcacat aaattccggc cattccttaa atggatgagg
     4501 aacgtgatcg ataaacagtt cgatcaattg attcccctac taaaagagca tgatatttta
     4561 gtatcgacca actcggaatt tgctgtcgca agtatcgccg agtattgcaa gaagcccttg
     4621 atacgtaccg cctacgcccc gttcttgccg ggtaagaaga ttcccccggc cgtattgcca
     4681 tttcccaaac ccaacccgat catcaccccc gccatcctat ggaagttgat gaaccggatg
     4741 accaatttca tggttaaaga cacgatcaac aagaaccggg ctaaatacgg actcgctccg
     4801 attcgtaatt ttggttatca tgcgggagag cgttcatata attacttgct tttcagccag
     4861 cacctaggaa acatagatcc ggactggacg ttcaaatggt cgatcggagg ctattgcttc
     4921 aatgacactt ttcaatatga tgagaaggct tacgaggaaa tgatatcatt cgtcgacagc
     4981 gctcaaagcc ctattatctt ttttaccctc gggagttgta gttccaagga tggtaaccgt
     5041 tttagcaaag ccttggtcga tatttgcaag aaacatgatt atcggctcat catcggttcc
     5101 ggttgggcaa agacaggaat caccttacaa gcggataaac atttattctt aatgaagcag
     5161 ccggttccgc ataatttgat atttcggcac tgcgacggcg ttatccatca tggaggttgc
     5221 ggcacgaccc atagcgtagg aagggcggga aagccccaat taatcactcc gttgattatc
     5281 gatcaaccct attggtccta tcgaatccac caattagggt tagggccgga agggcttaag
     5341 atagcgaaag cgagcgagca ggagatcgaa cgcaaggttt gtgatctggt caccaatccg
     5401 ctttataaga aaaatgccgc tcatataggc gagatgatcc aaaaagaggg aggtataaag
     5461 aatatatgcg actctatcga aagatttcaa tgaggcataa gttcctatta gattttatta
     5521 catgttacaa aacacaattt agcacttaaa ctattgacaa ataatagtaa ctccgtatat
     5581 ttgcatcgtg gttttttcat aatagtatta gatttaaggt taacaaagtt tggt
//
