LOCUS       c00285_gnlPro..         2802 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HEKCBFPP_285.
ACCESSION   c00285_gnlPro..
VERSION     c00285_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:46:57
            Original ID  :: gnl|Prokka|HEKCBFPP_285
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2802
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2802
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[507:2800]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      508..2800
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2802
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2802
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(508..1236)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.8;
                     E-value: 1.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg285_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNPDTWKMTEE
                     NGAVWKSADALLNQALRYRPYWKNGGGITVSGGEPLLQIDFMLEFFKKAKEKGIHTVID
                     TAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILDMIRYLSDI
                     KKPIWIRHVLVPERSDKDEYLTRLAEFIHSLDNVEKVEILPYHTMGIYKWKELGLEYPL
                     EGIEPPTKERVENAKKILAI"
     CDS             complement(1247..2800)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg285_2"
                     /sec_met_domain="Gly_radical (E-value: 6.7e-43, bitscore:
                     132.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.4e-180, bitscore:
                     585.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFL
                     DIYIKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLG
                     QDGRSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYEN
                     DDVMRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPE
                     YKPITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRR
                     TFATGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVW
                     LLQTFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYG
                     AEQNGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGA
                     HHLNVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM
                     "
ORIGIN
        1 gggataggct ctctttgctg gctgactgtg gatgctggta caaaatcaga attcatgtta
       61 gagatttggg gattctccag aatattatga atttgtaaaa aaatcagctt ctctataaca
      121 tggtataaaa agatattact ctgattcact ttctcaaatt ttttcactga tttgttgatg
      181 caggagtaaa cataaacatc cgctataaaa actttgaaaa atcgaattta aatattatct
      241 ttttaatata ctgttatgga ttcattgtat tttctcataa aatcacagcc tttcggggaa
      301 tccccaaatt cctccaactg attttctgag ctgtcaacgc acagaactcc tgaagctaaa
      361 taaagcatat ttgtggcctc ggagcaaggt ttggatatga gcgctttaag cgaacattca
      421 aactgccgcg actaaagtcg caaatatgct ttatttagct tcaggagttc gtctgctttg
      481 cagcctcaca aatcagaaat tgatagatta aatagcaaga attttcttag cgttctccac
      541 tcgttctttt gttggtggct caattccctc cagtggatac tctaatccaa gttctttcca
      601 tttataaatt cccatcgtat gataaggcag gatttccacc ttttccacat tatcaagaga
      661 gtgaatgaac tcagctaatc tagtcagata ttcatcttta tcgcttcgtt ccgggacaag
      721 gacatggcga atccaaatcg gttttttgat atcggaaaga tagcgaatca tatcaagaat
      781 attctcgttg ctgcgaccag ttaaaacttt gtggtatttg ttatcaatgt gtttgatatc
      841 cactaataac aggtcagtat atttcatcaa ttcctgaaat ttagaaaaga aaggttcttc
      901 tcttgtaaaa ggtcctccag cagtatcaat aacggtgtgg attccttttt cttttgcttt
      961 tttaaaaaac tcaagcataa aatcaatctg taaaagaggc tctccaccac tgacagtaat
     1021 acctccgcca tttttccagt aaggacgata gcggagtgcc tgatttaaca gcgcatctgc
     1081 gcttttccat accgcaccat tttcttctgt catcttccat gtatctggat tgtgacagaa
     1141 ctggcaccgc aaagggcaac cctgcaaaaa aacaataaaa cgaactcccg gcccatccac
     1201 cgaaccaaaa ctttccgtag aatgaacaag agccattgta tttttattac attctgtcat
     1261 ggcatgttct tgcaattaca tctaactgct gttcttttgt tagagagatg aatttaactg
     1321 cataacctga aacacggatc gtaaagtttg cgtattcttc tttttccgga tgttccatcg
     1381 catcaatcag tttctcaaca ccaaatacat ttacgtttaa gtggtgagca ccctgtgcga
     1441 agtagccgtc cataacattt actaagttac caatacgttc ttcttcggaa tgaccgagtg
     1501 catcagggtt gattgtctgc gtattggaaa taccatctaa tgcccattca taaggaagtt
     1561 tagctacaga gtttaaggaa gctaagagac cgttctgctc tgcaccataa cttgggtttg
     1621 ctcctgggga aagtggttca ccggcttttc ttccatctgg aagggaacct gttgccttac
     1681 catatactac gttagatgtg atagtaagga tagaagttgt aggttcagaa tcacgataag
     1741 tatggaactt ctccagtttc ttcatgaatg tctgaagtaa ccatacagcg atatcatcgg
     1801 cgcggtcgtc atcatttccg tatttaggga agtcgccagt tgtttcataa tctacaacga
     1861 gtccttcttc gttacgaact gtcttaacct ttgcatattt gatagcactt aaggagtcta
     1921 ctacgtgtga gaatcctgcg atacctgtag cgaatgtacg acgtacattt gtatcgatta
     1981 aggccatcaa agcgtattca tagtaatact tgtcatgcat gtactggatg agatttaatg
     2041 tatttacata taaacctgct aaccagtcca tcatgatatc atatttgtgc attacttcat
     2101 catagtcaag atattcggaa gtaatcggtt tatattccgg tccaacctgc tgtccggtct
     2161 tttcatccac accaccattg atagcgtaaa ggagacattt tgcaaggtta gcgcgagcac
     2221 cgaagaactg catttcctta ccagtctgtg tagcagaaac acagcagcag atggagtaat
     2281 catcgcccca taccggacgc attacatcat cattttcata ctggattgaa cttgttgtaa
     2341 tagaaatatg agctgcgtaa tctctaaatg cttttggaag acgctcagag taaagtactg
     2401 tgaggtttgg ctctggggat ggtcccatat tttctaatgt gtggaggaaa cggtagtcag
     2461 tttttgtaac catggaacgg ccgtcctgtc caagtccgcc aacttcaagt gttgcccata
     2521 cagggtcacc ggagaagagc tggttgtaag ctgggatacg tccgaactta accatacgga
     2581 acttcatagt catatggtcg attaattcct gcgcttcttt ctctgtaagg attcccttat
     2641 ccatatcacg tttgatataa atgtcaagga aagtagcgat acgtccgata ctcatagcag
     2701 caccgttctg tgttttaatt gctgcaagat aaccaaagta tgtccactga acagcttctt
     2761 ttgcattaga agcaggctca gaaatatcat aaccatagct tt
//
