LOCUS       c00312_gnlPro..         6214 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BIOKPEKA_312.
ACCESSION   c00312_gnlPro..
VERSION     c00312_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:26:13
            Original ID  :: gnl|Prokka|BIOKPEKA_312
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6214
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6214
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2234:6212]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2235..6212
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6214
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6214
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             3..2090
                     /locus_tag="ctg312_1"
                     /transl_table=11
                     /translation="GEVVATAIRKQTTTLETLNADRVKLLPDPAGGSIESLVVTFAGVT
                     SNNELSSQYSVRGGSYDENIVYVNGLEVFRPLLIRSGQQEGLSFINPDMTEAVNFSAGG
                     FEARYGDKMSSVLDITYKKPKGFEGSASASLLGANAYVGSSSGKFTQVTGFRYKTGRSL
                     LKTTDTDAEYDPNFIDLQTYMTYQLAPKWEINFLGNLAINNYKFIPHTRETSFGTATNA
                     KKFKVFMSGQERDKFETLFGALTLKHNLNDNTELGLQASAFTSKEEEGYDIAGDYWLGD
                     AAEEGGGEIQKLSIARYNEHARNRLHSNIMNVGHYGIARIKNNTLKWGATVQLEKINDK
                     ISEWEKRDSAGYSLPQGGGNVNVIANLFSDNKLESTRISGYLQDVFKFRTKQGLFTLVG
                     GVRGSYWSYNREFIFSPRASIGFIPNFDQNLTFRLASGLYYQSPFYKELRTTVQDEHGN
                     SIIQLNKNLKSQRSIHVIAGGDYTFRASGRNFKVSADMYYKKLDNLNPYTVDNVKIRYY
                     GENCAQGHAMGLDVKFFGEFVPGTDSWISFSLMKAEQSIRGSEYVPMPNSQGYNVSLFF
                     QDYFPGYKRVKLNLKGVLSGGLPVTAPRTGYEDGYFRTPTYKRVDLGFSYQLAGGTDAI
                     MDRGFFRNLKNIWLGLDIFNLFDIKNTSSYYWITNIDNHQYAIPNYLTGRQLNIRLIVD
                     F"
     CDS             2235..2642
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 6.8e-28, bitscore:
                     85.9, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEEVLGLKCYNIEEVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALDEAESKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGK"
     CDS             2755..4308
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_3"
                     /sec_met_domain="CT_Btheta (E-value: 4.6e-292, bitscore:
                     957.3, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALRETARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIYAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGARVHST
                     KSGVTHFTAQTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             4340..5260
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_4"
                     /sec_met_domain="OAD_gamma (E-value: 3.3e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILINNQSNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGALGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             5285..5716
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             5718..6212
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg312_6"
                     /sec_met_domain="OAD_beta (E-value: 2.6e-53, bitscore:
                     169.8, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLGFIYLAVAK
                     EFEPMLLIPIGFGMLIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SS"
ORIGIN
        1 tgggagaagt cgtagctacc gccatccgaa agcagacaac cactcttgaa accctgaatg
       61 ccgaccgcgt caaactactc cccgaccctg ccggaggaag cattgaaagt ctggtcgtta
      121 catttgctgg cgttacttcc aataacgaat taagttcgca atactccgta cgcggcggca
      181 gctatgacga aaacatcgta tatgtgaacg gactggaagt gttccgtccg ctgctcatcc
      241 gttccggaca acaggaaggg ttgagtttta tcaacccgga catgacggaa gccgtcaatt
      301 tctccgccgg aggattcgaa gcccgttatg gggacaagat gagttccgtg ctggacatca
      361 cctacaaaaa accgaaagga ttcgaaggtt cggcttccgc cagcctgctg ggagccaatg
      421 cttatgtggg cagttcttcc ggcaagttca cacaggtgac cggtttccgt tacaaaacag
      481 gacgatctct tttgaaaaca accgatacgg atgccgaata tgatcctaat ttcattgatt
      541 tgcagactta tatgacatac cagcttgccc ccaaatggga gatcaacttt ctcggaaact
      601 tagccataaa caattataag ttcattcccc acacccgaga gacttctttt ggaacggcaa
      661 caaatgccaa aaaatttaaa gtattcatga gtggccagga acgggataag ttcgaaacgt
      721 tattcggagc actcacttta aagcataacc tgaacgacaa tacggaactc ggcttgcaag
      781 catccgcctt caccagtaag gaagaggaag gatatgatat tgccggtgat tactggctgg
      841 gagatgcagc cgaagaagga ggcggagaaa tacagaaatt atccatcgca cgctataacg
      901 agcacgctcg caaccgtctg cactccaata tcatgaatgt cgggcattat ggtatcgctc
      961 gcataaaaaa caatacgttg aaatggggtg ctaccgtcca gttggagaaa atcaacgaca
     1021 agatcagcga atgggaaaaa cgcgactccg ccggatactc gttaccgcaa gggggcggga
     1081 atgtcaacgt gatcgcaaac ctgttctcgg ataacaaact ggaaagcaca cgtatttccg
     1141 gttatttgca ggatgtattc aagtttcgta ccaaacaagg actgttcacg ctggttggtg
     1201 gtgtacgcgg cagctattgg agttataaca gggaatttat attcagcccg cgtgcttcaa
     1261 taggtttcat tccgaacttc gaccagaacc tgacatttcg gctggcttcc ggtctgtatt
     1321 atcagtctcc gttctataag gaattacgta caactgtgca ggacgaacac ggcaacagta
     1381 tcatacaact gaacaaaaac ctgaaatcac agcgttccat ccatgtcatt gcaggaggcg
     1441 attatacatt ccgggcatcc ggcagaaatt tcaaagtcag tgccgatatg tattataaaa
     1501 aattggataa cctgaacccc tatacggtgg ataatgtcaa aatccgatac tacggcgaga
     1561 actgtgcgca aggccatgcg atggggctcg atgtcaaatt cttcggcgaa tttgttccgg
     1621 gtacggactc ctggatcagc ttctccctaa tgaaagcgga gcaaagcatc cgagggagcg
     1681 aatatgtacc gatgcctaac agccaaggtt ataatgtctc gctctttttt caggattatt
     1741 tccccggata caaacgtgtc aaactgaatc tgaaaggcgt actgtccggc ggccttcccg
     1801 tcacagcccc acgtacaggc tacgaggacg gctatttccg tactccgact tacaaacgcg
     1861 tggacctcgg tttctcctac cagcttgccg ggggaacgga tgcaatcatg gatcgcggat
     1921 tcttccgcaa tctcaagaac atttggctcg gattggatat tttcaacttg ttcgacataa
     1981 agaacactag ttcctattat tggattacca atatagacaa ccaccagtac gcaatcccta
     2041 attacctgac cggaaggcaa ttgaatattc gacttatcgt tgatttttaa ccatatgatt
     2101 tcaaaaatat gctttttaaa catatccgga tcatgctgat aaatattaac gaattatttc
     2161 tctattaata aaaagagtgt acctttgcca acatagaaca aaaagcaata ttaaattact
     2221 aaattattat tgacatggaa cttacacata tcgaacattt gggtatcgca gtaaagagca
     2281 tcgaggcttg cctgccgtat tatgaagaag ttttaggact aaaatgctac aacatcgaag
     2341 aagtagcaga ccagaaagta aaaactgcat tctttaaagt aggccagact aagatcgagt
     2401 tgctggagcc gacaagtgaa gacagtacaa tcgctaaatt tatcgaaaag aaaggtgaag
     2461 gtatccacca cattgcattt gctgttcccg acgtacaggc agcattggac gaagccgaat
     2521 caaaaggtgt caagctgatc gacaaagctc ctcgtggagg tgcagaaggc ttgaacattg
     2581 cattccttca cccgaaatcg acatgcagcg ttctgaccga actttgcatg ccgggaaaat
     2641 aagaaacaat ggacaattga gaattgacaa ttatttgatg ctattcaatt ggcaattctc
     2701 aatcgtcaat tgccaattaa ataagttcat ttctaacaac aaatctatag tacaatgagt
     2761 aaccaacttg aaaaagtaaa ggagcttatc gcccttcgcg aaacagctcg tttaggtggt
     2821 ggagaaaaaa gaatcgaatc tcagcacaaa aaaggtaaat acaccgcacg cgaacgtatt
     2881 gccatgttgc tggatgaggg aagttttgaa gaattcgata tgttcgtaca acatcgttgt
     2941 acaaacttcg gtattgaaaa aactaagtat ttgggcgacg gtatcgtaac aggttacgga
     3001 acgatcgacg gacgtctggt atatatttat gctcaggatt tcaccgtatt cggcggtgct
     3061 ttatccgaag cattggccat gaagatctgt aaagtaatgg atcaggcgat gaagatgggt
     3121 gctcccgtta tcggcttgaa cgactcgggt ggtgcacgta tccaggaagg tatcaacgct
     3181 ttggcaggtt atgcagaaat cttccaacgc aacatccttg cttcaggtgt tattccacag
     3241 atttcaggta ttttcggtcc ttgtgccggt ggtgctgttt attctccggc tctgaccgac
     3301 tttacactga tgatggaagg cacttcttat atgttcctca ccggaccgaa agtggtgaag
     3361 accgttacag gtgaagacgt tagccaggaa aacctcggtg gcgcaagagt tcactccact
     3421 aaatcgggtg tgactcattt caccgcccaa acagaagaag aaggtttcga actgattcgc
     3481 aaactgttga gctatattcc tcaaaacaac cttgaagaag ctccttatgt agattgcaca
     3541 gacccaatcg accgtctgga agattctttg aacgatatta tccccgacag ccctactaaa
     3601 ccgtatgaca tgtacgaagt gatcggcgct attgtggaca acggtgaatt ccttgaaatc
     3661 cagaaagatt atgcaaagaa tatcatcatc ggtttcgccc gtttcaacgg acaatcggta
     3721 ggtatcgttg ctaatcagcc taagtatctg gctggtgtac tcgatagcaa cgcatctcgt
     3781 aaaggcgcac gtttcgttcg tttctgcgat gcattcaata ttcctatcgt atcgttggta
     3841 gacgtaccgg gattccttcc gggaacaggt caggaataca atggcgttat ccttcatggt
     3901 gctaaactgt tgtatgctta cggtgaagct actgtgccta aggttactat tacattgcgt
     3961 aaatcttacg gtggttcaca catcgtaatg agctgtaagc aacttcgcgg tgacatgaac
     4021 tacgcatggc ctactgccga aatcgcagta atgggtggtg caggagcagt agaagtattg
     4081 tacgcacgcg aagccaaaga tcaggaaaac cctgctcaat tccttgcaga gaaagaagca
     4141 gaatacacga aattgttcgc caatccttac aatgcagcta aatatggcta catcgacgat
     4201 gtgattgaac cgcgcaacac acgtttccgt gtgatccgtg ctttgcagca gttgcagact
     4261 aagaaattga gtaatccggc taagaagcat ggtaatattc cgttgtaatc ctacttttca
     4321 cattaaaaca agaacgatta tgaacaaaac caaaatcgga atattccttt ctttgctgtt
     4381 gctgattggt ctgacttctt gtggagagca aaagtcgaac aataagctgg tgctaaacga
     4441 aatcctgata aacaatcaga gtaattttca ggacgattac ggattgcaca gcgcatggat
     4501 tgaaatattc aataaatcat tcggtagcgc cgacctggca gcttgcttgc tgaaagtaag
     4561 cagccaaccg ggcgatacag ttacttattt tatcccgaaa ggtgatatac ttaccttagt
     4621 gaaaccacgc caacatgcac tattctgggc agatggcgaa cctaaccgtg gtactttcca
     4681 taccagcttc aagctgaatc cggaaacagc taattgggtt ggtctgttcg actccggcaa
     4741 aaagttgctc gaccagattg tagtgcccgc aggcgctctc ggtcccaatc aatcatacgc
     4801 tcgtgtaagc gacggggcag ctgaatggga agtaaaaagt ggaagcggtg acaaatacgt
     4861 gactccaagc accaacaaca agactcttga cagcaattcc aagatggaaa agtttgaaga
     4921 acacgatgct gatggcgttg gaatgtccat ttctgccatg agcgtagtat tctgcggatt
     4981 gattcttctc tttattgctt ttaagattgt aggaaaagta gctgttaatt tgagcaagcg
     5041 caacgctatg aaatctaaag gcattgacaa gcatgaagct aaggaactgt cacaggctcc
     5101 gggcgaagta tacgctgcta tttctatggc attacacgaa atgcaggatg aggtgcacga
     5161 cgtagaagaa acggtgctga ccatcacccg cgtaaaacgc agctactcac catggagttc
     5221 gaagatttac actttgcgtg aaactcctcc cagaaagtaa gtcacctttt aaaaagttaa
     5281 aacgatgaaa gaatataaat ataaaatcaa cggtaactca tataaagtaa ccatcggaga
     5341 tattgaagac aacatcgctc atgtagaagt gaacggtaca cactataaag tagaaatgga
     5401 gaaacagccg aagcctgttg cgaaacccgt gacagtacgc ccgatgccta atgctcctgc
     5461 tgctcctact caagtagtga aaccgaccgc tccgtctacc ggaaaatcag gagtaaaatc
     5521 tccgctgccg ggtgtgatcc tcgacatcaa agtgaatgta ggcgatactg taaagaaagg
     5581 acagaccatt atcatattgg aagccatgaa gatggaaaac aatatcaatg cggataagga
     5641 cggtaagatt actgccatca acgtcaacaa gggagactct gttcttgaag gtaatgacct
     5701 cgtaattatt gaataatatg ggagatttta taaacttttt aggaaataac ctcgccgact
     5761 tctggacata cacgggcttt gcaaatgcta ctgtagggca tgtcgttatg attcttgtgg
     5821 gtttggggtt catctatctg gcggtagcca aagagttcga accgatgctg ctgattccta
     5881 tcggcttcgg tatgctgatc ggtaatatac cttttaatat ggatgccgga ctgaaagtcg
     5941 gtatttatga agaaggttcc gtattgaata tattgtatca gggagtaact tccggctggt
     6001 atccgccgct catctttttg ggcatcggcg ccatgacgga cttctcggca cttatctcta
     6061 atccgaaatt gatgctgatt ggtgcagctg cacagttcgg tatcttcggt gcatatatga
     6121 tcgcgttaga aatgggcttc gatccgatgc aagccggtgc tatcggtatc attggtggag
     6181 cagacggccc gacggccatc ttcctttctt ccaa
//
