LOCUS       c00477_gnlPro..         3786 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BIOKPEKA_477.
ACCESSION   c00477_gnlPro..
VERSION     c00477_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:26:13
            Original ID  :: gnl|Prokka|BIOKPEKA_477
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3786
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3786
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3492]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3492
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3786
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3786
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..683
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg477_1"
                     /sec_met_domain="SucD_FumR (E-value: 1.5e-132, bitscore:
                     426.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GRKVVMSVTGIALVLFLTFHMAMNLVAIISADGYNMICEFLGANW
                     YALVATAGLAALFVIHIIYAFWLTMQNRKARGSERYAVVDKPKTVEWASQNMLVLGLIV
                     IVGLGLHLFNFWAKMQLPELMHNMGMHADTLTLAYAANGAYHIQQTFSSPVYVVLYLVW
                     LFALWFHLTHGFWSSMQSLGWNNKVWINRWKCISNIYSTIVVLGFALVVVVFFVKTLIC
                     GGAC"
     CDS             721..2700
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 202.3; E-value:
                     4.6e-62)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg477_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.2e-63, bitscore:
                     202.3, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.5e-38,
                     bitscore: 118.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRAREANVYRLAEVSNAIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQT
                     GQQLLLGAYSALSRQVNVGTVKLYTRYEMQDVVIVDGRARGIIAKNLVTGELERFAAHA
                     VVIATGGYGNAYFLSTNAMGCNCTAAISCYRKGAVFANPAYVQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKKEDAVKLQKGEIKGSDIPEEDRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSEAINRLGIDVVLQRYGNLFDMYEEITDVNPGE
                     LAKEISGVKYYNPMMIYPAIHYTMGGIWVDYELQTTIKGLFAIGECNFSDHGANRLGAS
                     ALMQGLADGYFVLPYTIQNYLADQITVPRFSTDLPEFAEAEKAVQAKIDKFMSIQGKES
                     VDSIHKKLGHVMWEYVGMGRTAEGLKKGIAELKEIRKEFETNLFIPGSKEGMNVELDKA
                     IRLYDFITMGELVAYDALNRNESCGGHFREEYQTEEGEAKRDDENFFYVACWEYQGDDE
                     KAPVLHKEPLVYEAIKVQTRNYKS"
     CDS             2737..3492
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg477_3"
                     /sec_met_domain="Fer4_7 (E-value: 2.2e-13, bitscore: 38.5,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 1.6e-26, bitscore: 80.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.7e-08, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQAGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIAKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFIIAKLKD"
ORIGIN
        1 taggaaggaa agtggtgatg agcgttaccg gtatcgccct tgtcctgttt ctaacatttc
       61 acatggcgat gaacttggtt gcaatcatct cggctgatgg ttacaacatg atttgtgagt
      121 tcctgggagc aaactggtat gcgttagtgg ctaccgcagg actagccgct ctttttgtga
      181 ttcacatcat ctacgcattc tggctgacaa tgcagaatcg caaagcgcgt ggtagcgaac
      241 gctatgcagt ggttgacaaa ccaaaaactg tagaatgggc atctcaaaac atgttggtgc
      301 tgggtctgat cgttatcgta ggtcttggtt tgcacctctt caacttctgg gcaaaaatgc
      361 agttgccgga actgatgcac aacatgggca tgcacgctga tacactgacg ctggcttatg
      421 ccgctaacgg tgcttatcac atccagcaga ctttctctag cccggtttac gtagttcttt
      481 acctcgtttg gctgtttgct ttgtggttcc acctgactca cggtttctgg agctctatgc
      541 aatcactggg atggaacaac aaagtatgga tcaatcgttg gaaatgtatt tctaacatct
      601 attctacgat tgttgtactc ggtttcgcac tggtagtagt ggtattcttc gtgaaaacac
      661 tgatttgtgg cggtgcttgc taaatagtaa attgtaaatg attaaattgt aaataatatt
      721 atgatcaaga tagattcaaa aattccagaa ggcccggtgg ctgagaaatg gaccaactat
      781 aaagctcacc agaaattggt gaaccccgct aataaacgtc gtttggacat catcgttgtg
      841 ggtacgggtc tggcaggtgc ttctgccgct gcttcacttg gtgaaatggg tttcagagta
      901 ttcaatttct gtattcagga ttctccccgt cgtgcacact ctatcgctgc acagggtggt
      961 atcaatgctg caaagaatta tcaaaatgat ggtgactccg tttaccgtct gttctacgat
     1021 actgtaaaag gtggcgacta tcgtgcccgt gaagcaaacg tatatcgttt ggctgaagtg
     1081 tctaacgcta tcattgacca atgtgtagcg caaggtgttc ctttcgctcg cgaatatggc
     1141 ggtacactag ataaccgttc tttcggtggc gcacaggtat cccgtacttt ctacgctaaa
     1201 ggccagactg gtcagcagtt gctgctgggt gcttactctg cattgagccg tcaggtaaac
     1261 gtaggtactg ttaaactgta tacccgctac gaaatgcagg atgttgtcat cgttgacgga
     1321 cgtgcccgcg gtatcatcgc aaaaaatctt gtaacaggcg aattggaacg tttcgccgct
     1381 cacgctgtag taatcgctac tggtggttat ggtaacgctt acttcctgtc tactaacgct
     1441 atgggttgta actgtacagc tgctatctct tgctaccgca agggtgctgt attcgcaaat
     1501 cctgcatacg ttcagattca cccgacttgt attccggtac atggtgacaa gcagtctaag
     1561 ctgactttga tgtctgaatc tctccgtaac gacggtcgta tctgggttcc gaagaagaaa
     1621 gaagatgctg taaaactcca gaaaggcgaa atcaaaggaa gtgatattcc ggaagaagac
     1681 cgcgactatt atttggaacg ccgctatccg gcattcggta acctggttcc gcgtgacgtt
     1741 gccagccgtg ccgctaaaga acgttgcgac gctggtttcg gtgtaaacaa cacaggtttg
     1801 gccgtattcc tcgacttctc tgaagctatc aaccgtttgg gtatcgacgt tgttcttcaa
     1861 cgttatggta acctcttcga tatgtacgaa gaaatcactg acgttaatcc gggcgaactg
     1921 gctaaagaaa tcagtggtgt gaaatattat aacccgatga tgatttatcc ggctatccac
     1981 tatacaatgg gtggtatctg ggtagactac gaactgcaaa ccactatcaa gggtctgttc
     2041 gctatcggtg aatgtaactt ctccgaccac ggtgcaaacc gcctcggtgc ttctgcattg
     2101 atgcaaggtt tggctgacgg ttacttcgta ttgccttaca ctatccagaa ctatctggca
     2161 gaccagatca ctgttcctcg tttctctacc gatcttcctg aatttgctga agctgaaaaa
     2221 gcagttcaag ctaagattga caagttcatg agcatccagg gtaaagaatc tgttgattct
     2281 atccacaaga aactgggtca tgtcatgtgg gaatatgtag gtatgggacg tacggcagaa
     2341 ggcctgaaga aaggtatcgc cgaactgaaa gaaatccgca aggaattcga aacgaacttg
     2401 tttatccccg gttctaagga aggtatgaac gtagagcttg acaaagcaat ccgtctgtac
     2461 gacttcatca ctatgggtga gcttgttgct tacgacgcat tgaaccgtaa cgaaagttgt
     2521 ggtggtcact tccgtgaaga ataccagact gaagaaggag aagcaaaacg tgatgacgaa
     2581 aacttcttct atgtagcatg ctgggaatat caaggtgacg atgaaaaggc tccggtattg
     2641 cacaaagaac cgctggtata cgaagctatc aaagttcaga ctcgtaacta caagagctaa
     2701 tcggtgaagt taaacattta aaagaattag aagaaaatgg ataaaaatat atcatttaca
     2761 ctgaaggtat ggcgccaagc tggtccgaaa gctaaaggtg cttttgaaac ctaccaaatg
     2821 aaagatatcc ccggtgatac ttccttcctc gaaatgctgg atattctgaa cgaacagctc
     2881 atcagcgaaa gaaaagaacc ggtagtattc gatcatgact gccgcgaagg tatctgcggt
     2941 atgtgttctc tttacatcaa cggacacccg cacggtcctg caacaggtgc tactacttgc
     3001 caaatctata tgcgtcgttt caacgacggt gacaccatca ctgttgaacc ttggcgttcg
     3061 gctggtttcc ctgtcatcaa agacttgatg gtagaccgta ctgcatacga caagattatg
     3121 caagctggtg gttatgtaag cgtacgcaca ggtgctccgc aggatgctaa cgctatcctg
     3181 atcgcgaaac cgatcgctga cgaagctatg gatgctgctt cttgtatcgg ttgcggtgct
     3241 tgtgtagctg catgtaagaa tggttctgct atgttgttcg tttctgcaaa agtcagccag
     3301 ttgaacttgc tgccgcaagg taaaccggaa gctttgcgtc gggcaaaagc tatgttgtca
     3361 aagatggacg aactgggatt cggtaactgt acaaacactc gtgcttgtga agctgaatgt
     3421 ccgaagaata tttctatcag caacatcgct cgcttgaacc gcgacttcat catcgcaaaa
     3481 ttaaaagact agaaactgct agatttcaat agcgagagct taatttgatt aatgacagaa
     3541 tcccctgccg gctagtccga caggggattt ctttatgtat gagtagatca caattattat
     3601 taatacggat cagtaacatg aacttttaat tcataagttt caccgatttc accttatagc
     3661 cagaactgtt atgaatgaga gaattacggg tgaaagcgag gattgaaata aggtaaaatc
     3721 attcgttacc gctgtaagcg tctccgcgat tctatcttgt cgcccttttt tcattcttat
     3781 attttt
//
