LOCUS       c00492_gnlPro..         3652 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BIOKPEKA_492.
ACCESSION   c00492_gnlPro..
VERSION     c00492_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:26:13
            Original ID  :: gnl|Prokka|BIOKPEKA_492
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3652
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3652
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[373:3346]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      374..3346
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3652
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3652
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             374..2602
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg492_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTDARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2618..3346
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg492_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3374..3652)
                     /locus_tag="ctg492_3"
                     /transl_table=11
                     /translation="DTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLV
                     SELLQRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
ORIGIN
        1 aaaactgggt gtttgttttg caatctgctg attttcagcg tttgttgcgg agagacaggg
       61 attcgaaccc cgggtacctc gcagtacaac ggttttcaag accgccgcaa tcgaccactc
      121 tgccacctct ccaaaacttc tttatcagaa gtgcttttcg ttaaaagcgg tgcaaaggta
      181 cgaatatatt ttaaacttac aagagttttc acaaaaaaaa atcacgaaca tcatttccaa
      241 agaatagcaa tatctaggta ttttatgttg aaaagcataa aatagagcat atttatgctg
      301 attatatgaa aaaacgttgt aactttgtgc gctctaaaga aaaggataac tacaacctct
      361 aattttatca tttatggaat taaataagat ctttaaagac ggtctttgga gcacagaaat
      421 caacgtaaga gatttcgtta gtcacaacat cactccgtat tatggagatg cttctttcct
      481 cgaaggacct accgaacgca caaaagctgt atggaaccgc tgcctcgaag cactggcaga
      541 agaaagagcc aataacggcg tacgctcact ggataatgtc accgtctcaa ccatcacttc
      601 tcataaagcc ggatatatcg acaaagaaaa tgaactgatc gtcggtctgc agacagacga
      661 acttctgaaa cgtgccatca agcctttcgg aggtatcaac gtggtgagca aagcctgcca
      721 cgaaaacggc gtggaagtgg acgaccgtgt aaaagatata ttcactcact accgcaagac
      781 gcacaatgac ggagtatttg acgtatatac cgaagaaatc cgttcgttcc gttctctggg
      841 attcctcacc ggacttcctg acaactatgc ccgcggacgt atcatcggcg actaccgccg
      901 tatggctctc tatggtatcg accgactgat tgaggcaaaa aaagaagact tgcgtaacct
      961 taccggtccg atgaccgatg cccgcatccg cctgcgcgaa gaagtggcag agcaaatcaa
     1021 ggcattgaag gacatgaagg taatgggcga atactacgga ctcgacttga gccgtcctgc
     1081 ctacaccgca caagaagcag tacaatgggt atacatggct taccttgctg ccgtaaaaga
     1141 acaagacggt gctgccatgt cactgggtaa cgtctcttcg ttcctcgaca tttatatgga
     1201 atatgaattg agcaaaggaa ccatcacaga atctttcgca caggagctga tcgaccagtt
     1261 tgtcatcaaa ctgcgtatgg tgcgccacct gcgtatgcaa tcttacaacg acatctttgc
     1321 cggcgacccg acatgggtaa ccgaatctct gggcggacgc ctcaacgacg gacgcacgaa
     1381 ggtaacaaag acttctttcc gcttcctgca aacactctac aacctcggcc cttcaccgga
     1441 accgaacctg actgtattgt ggagcccgga actgccggaa ggattcaaag aattctgcgc
     1501 aaaagtatct atcgacactt cttccatcca atatgaaaac gacgacctga tgcgtgaagt
     1561 acgccagtct gacgactacg gaatcgcgtg ctgcgtatct tatcaggaaa tcggaaaaca
     1621 aatccagttc ttcggcgcac gttgcaacct ggctaaagcc ctgctgcttg ccatcaacgg
     1681 tggacgttgc gaaaacaccg gcacggtaat ggtgaaaaac atccccgtac taaccagcga
     1741 cacactgaac ttcgaagaag taatgagcaa ctacaagaag gtattgacag aaatcgcccg
     1801 tgtttacaac gaagcgatga acatcatcca ttatatgcat gataagtatt actacgaaaa
     1861 agcccaaatg gctcttgtag ataccaaccc gcgcatcaac cttgcttacg gtgtagccgg
     1921 actttctatc gccctcgatt cactgtcggc catcaaatat gccaaagtga ctgcacgccg
     1981 caatgacatc ggtctgacag aaggtttcga tattcaaggc gaattccctt gcttcggtaa
     2041 tgacaacgac aaggtagacc acctgggtgt tgacctggtc tatttcttca gcgaagaatt
     2101 gaagaaactt ccggtttaca aaaatgcccg tcctactctg tctttgctca ccatcacttc
     2161 caacgtgatg tatggcaaga aaacaggtgc tactcccgac ggacgcgcca aaggtgtcgc
     2221 ttttgctccg ggagccaacc cgatgcacgg acgtgacaag aacggtgcta tcgcctcttt
     2281 gagttccgta gcgaaattgc gttaccgcga ctcacaagac ggtatcagca acaccttctc
     2341 catcgttccg aaatcactgg gagctactga agaggatcgc gtagaaaatc tggtgacaat
     2401 gatggatggt tacttcacca aaggcgctca ccacctgaac gtgaacgtac tgaaccgtga
     2461 aatgctttac gatgccatgg aacatccgga aaaatatccg caactcacca tccgtgtttc
     2521 cggttatgcc gtaaacttcg tgaagttgag ccgcgagcat caattggaag ttatcagccg
     2581 tagcttccac gaacgtatgt aatcagatct actcaatatg acgataaacg tacattcata
     2641 cgaaagtatg ggaacattcg acgggccggg cttacggctc gtcgtttttc ttcaaggatg
     2701 caatttccgc tgcctgtatt gtgccaaccc cgacacgata gccggaaaag gaggcacacc
     2761 taccccaccg gaagaaatcg tccgcatggc tatgagccaa cgtccttttt tcgggaaacg
     2821 cggaggagtc actttctccg gaggagaacc tacgtttcag gccaaagcac tagtcccttt
     2881 agtgcgtgaa ctgaaagaaa agggaataca tgtatgcata gacagcaacg gcggtatctg
     2941 gaacgaagaa gtagaagaac ttttcaaact gacggatctt gtattgctgg atattaaaga
     3001 gttcaatccg gcacgccatc aagcactgac cggaagaagt aacgagcaaa ccatccgcac
     3061 ggcagcatgg ctggaagaaa acgagaaacc tttctggctg cgttatgtat tagtgcccgg
     3121 atacagcgat ttcgaagagg atatccgccg cttgggagag gctctgggaa aatataagat
     3181 ggtgcaacga gtggaaatcc tgccttatca tacattaggc gtacataagt atgaagcgat
     3241 ggagcaggaa tacaagctga aagacgtaaa agaaaatacc cccgaacaac ttgaaaaagc
     3301 cgcggaggta tttaaagagt atttcactac tgtggtggtc aattaaccac cacagtaata
     3361 aataggataa aaatcaccat gtctctgctt tcacctcgaa acagggacac tctttgatcc
     3421 gctcccaagg gtcaacaatt ccattataat tagtatcagg gctcaagtcc cggtgaccga
     3481 ccacctttgc atccggataa cgctgcaaca actcgcttac cagacgatgc atcgcctctt
     3541 tctggcggga agtacgtgta tccgctgcct ttccgttctc atccagcccg ccttcatagc
     3601 acacccctaa actaatcgca ttaaaaccgc gtgcatgagc acccaccgta tc
//
