LOCUS       c00148_gnlPro..         6300 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ONJOAAGF_148.
ACCESSION   c00148_gnlPro..
VERSION     c00148_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:45:54
            Original ID  :: gnl|Prokka|ONJOAAGF_148
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6300
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..144)
                     /locus_tag="ctg148_1"
                     /transl_table=11
                     /translation="MNNAVSYAMDSQVIFQMFFSIVCIAAAIGVSILVLRFMRKFIDFL
                     LQ"
     protocluster    1..6300
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1247:6220]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1248..6220
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6300
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6300
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             369..557
                     /locus_tag="ctg148_2"
                     /transl_table=11
                     /translation="MPMKPKEMIRLLKKNGFIKISQNGSHVKMKNFKTGKQTTVPLHSK
                     DLKKGLEDAILKQAGLK"
     CDS             603..992
                     /locus_tag="ctg148_3"
                     /transl_table=11
                     /translation="MKKLFYPAVFHIAEEGGYWVTFPDFPECLTQGENMQEAYDMAIDA
                     LGLILTDDINDHKELPEPSNITHVDDGVVVIIPYDYLEYNRKYHNKSIKKTLTIPEWLN
                     NEALRLNLNFSQILQDALLNKIQSR"
     CDS             complement(1248..2660)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_4"
                     /sec_met_domain="HMGL-like (E-value: 4.8e-44, bitscore:
                     139.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 2.5e-59, bitscore:
                     189.5, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEIKKKPVKIVETVLRDAHQSLIATRMTTEEMLPIVDKMDQVGY
                     HAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYKPYPDDVVE
                     YFVQKSIANGIDVIRIFDCLNDLRNLQTAVDATKKEGGHAQIALSYTIGDAYTLDYWKK
                     TAKNIEEMGADSICIKDMAGLLVPNEATRLVTALKQATDIPIELHTHYTSGVGGMTYLK
                     AVEAGVDIIDTAISPFAMGTSQPATEVMAETFRGTEFDTGFDQKLLGEIADYFRPIREK
                     YLANGLMSPKVLGVNIKTLMYQVPGGMLSNLVSQLEQQGASDKFYEVLEEVPKVRKDFG
                     EPPLVTPSSQIVGTQAVLNVLTGERYKMITNESKALLRGEYGQTVKPMNKEVQKKAIGD
                     EKPITCRPADLLEPELSKIEAEMSQWKEQDEDVLTYALFPQVATEFFKYRAAQENKIDP
                     AKADVKNGAYPV"
     CDS             complement(2818..3942)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_5"
                     /sec_met_domain="OAD_beta (E-value: 7.3e-158, bitscore:
                     514.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVVETMTNLAAQTGFAFLKPGNFIMILVALVFLYLAIAKGYEP
                     LLLVPISFGMLLVNLYPSIMEEGGLLHYFYLLDEWSILPSLIFLGVGALTDFGPLIANP
                     ASFLLGAAAQFGIYSAYFLAMLMGFNGKAAAAISIIGGADGPTSIFLAGKLGQTDLMGP
                     IAVAAYSYMSLVPIIQPPIMKLLTTEKERQIKMEQLRTVTKTEKIMFPIVVTVVVCLIL
                     PTTAPLVGMLMLGNLFRESGVVKQLSETASNALMYIVVIILGTSVGATTSAEAFLNVST
                     IKIVVLGLVAFAVGTAAGVLFGKLMCKVTGGKVNPLIGSAGVSAVPMAARVSQKVGAEA
                     DPSNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             complement(3976..4344)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.6; E-value:
                     4.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_6"
                     /sec_met_domain="Biotin_lipoyl (E-value: 5.5e-22, bitscore:
                     66.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYRITVNGVAYDVAVEELGAGEASAAPVSTPAAVPAPAKKAAP
                     KTSGSAGAVKITAPMPGKIVAVKAQAGASVKKGDAVLVLEAMKMENEICAAQDGVIASV
                     EVAVGDMVEGGDVLATMN"
     CDS             complement(4439..4783)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_7"
                     /sec_met_domain="OAD_gamma (E-value: 3.6e-28, bitscore:
                     87.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFEQALLNTLMGMGTVFAVLIFISLLISLFVYIPSIERALKNR
                     SSKKEKKAAQEERPAPKRPILEEVVEEEELVDDGELVAVITAAIMAANGGAAVSADKLV
                     VRSIKRVKRR"
     CDS             complement(4796..6220)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_8"
                     /sec_met_domain="CT_Vparvula (E-value: 3.5e-138, bitscore:
                     450.3, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNSKMNARERIDYLFDDHSFVEVGGYVSARNTDYNLGAKKVEGD
                     GVITGYGVINDRMVYVYSQDASALGGSVGEMHAKKIASLYDLAMKTGAPIIGMLDCAGL
                     RLQEATDALHAFGTIFNKQAQASGMIPQISAVFGMCGGGSAVMSAMSDFTLMEKENGSL
                     FINSPNALAGNEESRQNTAGAQFQSEQAGNVDFVCETEEALLDQLRILVDVLPSDYEAE
                     DVYFDNDDDLNRVIPELNDAAYDAAYILKSISDNNMYIETKAAYAKEMVTAFIRLNGET
                     VGAVANQPVDGESVLTRNGLEKAERFIKFCDAFSIPLLTVTNVTGLRANAHEERKQALA
                     LAKFTTTLAQVSVPRINLIAGEAYGTAAEVMNSKAIGADFVLAWPQASVGMMDAEQAVR
                     IMYAEEINTDKNKTALIAEKTAEYAEGQSSALAAAKRGYIDDIIEPDATRKRLIAAYEM
                     LYGKKVTPVTKKHTAV"
ORIGIN
        1 ctattgtaac aaaaaatcta taaacttcct cataaagcga agaaccaaaa tggatacacc
       61 tatagctgcc gctatacata caattgaaaa aaacatctga aaaatcacct gactatccat
      121 agcatatgaa actgcgttat tcattgtatc tccccctgac tatcttttga tacttcattt
      181 taccatatct ctgtattttt atcaaaaatt tttcattttc aaaattacat tttttatgtt
      241 attttacatt tattagttgt ttttcttctt aaatatgata tactattcct caccagttgt
      301 atcaccgagt tcttgacaca cgtatattat acgtattata atataattat agaaaggggt
      361 ggcatactat gccaatgaaa ccaaaagaaa tgataagatt attaaaaaag aatggtttca
      421 taaaaataag tcaaaatggt tcccatgtga aaatgaagaa ttttaaaact ggcaaacaaa
      481 caacagttcc tcttcatagc aaagatttaa aaaaaggact agaagatgcc atcctaaaac
      541 aagcgggctt aaaatagcct tatttcatct ataataactc acatacattg gaggtatcca
      601 ttatgaaaaa attattttat cctgctgtat ttcatattgc agaagaaggc ggttattggg
      661 tgacttttcc tgactttccc gaatgtctaa ctcagggaga aaatatgcaa gaagcctatg
      721 atatggctat agatgcttta ggacttatct taactgatga cattaacgac cataaagaac
      781 ttccagaacc gtctaacatc acacatgttg atgatggagt tgtcgtaatc atcccatatg
      841 attatttgga atataatcga aagtatcata ataaatctat caaaaaaact ttaacaatcc
      901 ctgaatggtt gaataacgaa gcattgcgat tgaatttaaa cttttcacag attttgcagg
      961 atgctctttt aaacaaaatt caatctcgtt aatggctata attctttcat ttggtaatac
     1021 tgcaatcata gattataata tacttaacaa ggcatctaat acgatagctt cacctatcca
     1081 tttccggcaa gtttaggtta aaaaacagaa cccccacaac tttatagata gcaaatgtga
     1141 tgtcggagca agtaaaaatg cggctgagca ttttctacgc agcgaaatct tcacatgtgc
     1201 tatctataaa gttgcggggg ttcgtctgtt tttaatctaa atcaatccta caccggatat
     1261 gctccattct ttacatcggc ttttgcagga tcaatcttat tctcctgtgc cgctctgtat
     1321 ttaaagaatt cggttgctac ctgtgggaac aatgcgtatg taagcacatc ttcgtcctgt
     1381 tctttccact ggctcatctc tgcctcgatc ttagataatt ccggttcaag aaggtcggct
     1441 ggacgacagg taattggctt ttcatcgccg attgcctttt tctgtacttc tttattcatt
     1501 ggttttacgg tttgaccgta ttctccacga agaagggctt ttgactcgtt tgtaatcatt
     1561 ttataacgct ctccggtaag tacatttaat acagcctgtg ttccgacaat ctgactggac
     1621 ggtgtaacta atggtggttc accgaaatct ttacgtacct ttggcacttc ctctaatacc
     1681 tcatagaact tatcagatgc gccctgctgc tctaactgag atacgaggtt ggaaagcatt
     1741 cctcccggta cctgatacat taacgtcttg atatttacgc caagtacctt tggactcatt
     1801 aaaccatttg ccagatattt ctcacgtatt gggcggaaat agtctgcaat ctcgcctaaa
     1861 agcttctggt caaatcctgt atcaaattct gtaccgcgga atgtttcagc cataacttct
     1921 gttgccggct ggcttgttcc cattgcaaat ggagatatgg ctgtatcaat gatatcgact
     1981 cctgcttcta ctgcttttaa gtaagtcatt ccaccgacac cacttgtata gtgtgtatga
     2041 agttcgatag gaatgtctgt tgcctgcttt aatgcagtta caaggcgagt tgcttcattc
     2101 ggcactaaaa gtcctgccat atctttgata cagatagaat ctgctcccat ctcctcaata
     2161 ttcttagcag tctttttcca gtagtctaat gtatatgcat caccgattgt ataggaaagg
     2221 gcgatctgcg catgtccgcc ctctttcttt gttgcatcta cagcagtctg taagtttcta
     2281 aggtcattta aacagtcaaa aatacgaata acatcaattc cgtttgcaat agatttctgt
     2341 acgaaatatt ctacaacatc gtctggatat ggcttatatc ctaagatgtt ctgtccacgg
     2401 aataacatct gtaactttgt attcttaaag ccatctctta atttacgaag tctctcccat
     2461 gggtcttcct ttaagaaacg caggcaggca tcaaatgttg cacctcccca gcattctact
     2521 gcatgataac caacctgatc catcttatct acgattggca gcatctcctc tgttgtcatt
     2581 cttgtagcaa tcagagactg atgtgcgtca cggagaacgg tttctactat tttaaccggt
     2641 tttttcttaa tttcggccat aatatctcct tttatttcaa atcacgtaac tatttgcgct
     2701 tacaactgtt attatctaaa attcaacttg taaactattt ataaatatcc cctgtaacat
     2761 tttttacaga ttatagccct ataaataatg aattttaaat aatcaaataa ttctaaatta
     2821 tactccaaaa atcgccataa atgttccggc tgctaccgct gtaccgataa ctccggcaac
     2881 gtttggtccc atcgcatgca tcagtaagaa gtttgatgga tctgcttccg caccaacttt
     2941 ctgggataca cgggctgcca taggaacggc agataccccg gcagaaccaa ttaatggatt
     3001 taccttacca cctgtaacct tacacataag tttaccaaac aggacacctg ctgcagtacc
     3061 aacagcaaaa gctacaagac ctaatactac aatcttgatt gtgctgacat ttaagaatgc
     3121 ctctgcactt gttgtcgcac caacggatgt accaagaata ataactacga tgtacataag
     3181 agcgttagac gctgtctctg aaagctgttt tactacgccg gactctctaa aaaggttacc
     3241 gagcatcagc ataccaacta atggtgctgt tgtaggaaga atcagacata caacaactgt
     3301 tacaacgata gggaacataa ttttctctgt ttttgtaacg gttcttaact gttccatctt
     3361 aatctgacgt tccttttctg tagtaaggag tttcatgatc ggtggctgaa taattggtac
     3421 aagtgacata taagaatatg ccgctaccgc aattggtccc atcaagtctg tctgcccaag
     3481 ctttccggca aggaaaatag aagtaggacc atcggctcca ccaataatgg aaattgctgc
     3541 tgctgccttt ccgttaaatc ccataagcat tgcaaggaaa tacgcgctgt aaataccaaa
     3601 ctgcgccgca gcccccagca agaaacttgc tggattggca atcagcggac cgaagtctgt
     3661 tagtgctcca acgcctaaga aaatcagtga agggagaata ctccattcat ctaataaata
     3721 aaaataatgt aataatccgc cttcttccat aatggacgga tataagttaa ccagcagcat
     3781 accaaaagaa atcggcacta acaaaagtgg ctcatatcct ttggcaatgg ccagatataa
     3841 gaagactaaa gcaaccagta tcataatgaa gttacctggc tttaagaatg caaatccggt
     3901 ctgtgctgcg agattcgtca ttgtctctac aacataactc ataaatcttc ctccgtttta
     3961 ttttgcaaat atgctctagt tcattgtagc cagtacatct ccgccttcta ccatatctcc
     4021 aactgctact tccacacttg cgataacacc atcctgtgct gcacagattt cattctccat
     4081 cttcatagcc tctaatacaa gaacagcgtc gcccttcttt acagaagcac ctgcctgtgc
     4141 ctttactgct acgatcttac ctggcattgg tgctgtaatc tttacggcac cggcacttcc
     4201 ggaagtcttt ggggcagctt tcttagctgg agccggaacg gctgctggtg tacttaccgg
     4261 cgctgcactt gcttctcctg cacctaattc ttctactgct acatcataag caacaccatt
     4321 aactgtaatt ctataatttt tcattgtaaa tcctcctgaa tgattttatg tacttctaaa
     4381 atctttctat agctaacttt tcaaaacttt ataaaaagat tccaagaata catttttgct
     4441 atcttctctt cactctcttg attgaacgaa cgaccaactt atctgcactg actgctgctc
     4501 ctccattcgc tgccatgatt gccgctgtga ttactgcgac aagttcacca tcatccacaa
     4561 gttcctcttc ttccacaact tcttcaagaa tcggacgctt tggagccggt ctttcttcct
     4621 gcgcagcctt tttctctttc ttagaagaac gattctttaa cgctctttca atagatggga
     4681 tatatacaaa aagagaaatc aataaagaaa tgaaaatcag tactgcaaat acagttccca
     4741 tacccatcaa agtatttaat aaggcttgtt caaatacgtt catactctca cctcactata
     4801 ctgccgtatg cttctttgtc actggagtta ctttctttcc gtaaagcatt tcataagcag
     4861 cgataagacg ctttcttgtt gcgtccggct cgatgatgtc atctatatat cccctctttg
     4921 cagctgctaa tgcactgctc tgtccttctg catattccgc agttttctct gcgataagtg
     4981 ctgtcttatt cttatctgta ttgatttcct ctgcatacat gatacgaact gcctgttctg
     5041 catccatcat tcctacagat gcctgtggcc atgccagaac aaagtcagct ccgatagctt
     5101 tagaattcat tacttccgct gcagttccat aagcctcgcc tgcaatgaga ttaatacgag
     5161 gtacactaac ctgtgcaagt gttgtcgtaa acttcgctaa tgcgagcgcc tgctttctct
     5221 cttcatgtgc gttggcacga agtcctgtca cgttcgttac agtaagaaga ggaatcgaaa
     5281 atgcatcaca gaacttaata aatctttctg ctttttcaag accatttctt gttaaaacgg
     5341 actcaccatc tactggctgg tttgcaaccg cacctactgt ctctccattc agacggataa
     5401 atgcagttac catctctttg gcataggctg cctttgtctc tatgtacata ttgttgtcag
     5461 aaatagattt taaaatgtaa gcggcatcat atgcggcatc atttaactca ggaataaccc
     5521 tatttaaatc atcatcgtta tcaaagtaaa catcttctgc ttcataatca gaaggaagta
     5581 catcaacgag aatacgaagc tggtcaagaa gtgcttcttc tgtctcacat acaaaatcta
     5641 cgttaccagc ctgctcagac tggaactgtg ctcctgccgt attctgacgg gattcttcat
     5701 tccccgctaa tgcattcgga ctgttgataa agagactgcc gttctccttc tccatcaatg
     5761 taaaatcaga catagcggac attacggcac ttcctcctcc acacattcca aatactgcag
     5821 aaatctgtgg aatcattcca gatgcctgtg cctgcttatt aaaaatcgtt ccaaaagcgt
     5881 gaagggcatc tgtcgcttcc tgtaatctaa gtcctgcaca atccagcatt ccaatgattg
     5941 gcgcgcctgt tttcatcgcc agatcataaa gggaagcaat tttcttcgca tgcatctcac
     6001 ctacggaacc tccaagtgcg gaagcatcct gactgtaaac ataaaccatg cggtcattaa
     6061 taactccgta tccagtgata acaccatcac cttctacttt ctttgcaccg agattataat
     6121 cggtattacg agcggaaaca tatccaccaa cttccacaaa actgtggtca tcaaacagat
     6181 aatcaatgcg ctctctggca ttcatttttg aattacccat attctttcct ccataaaaat
     6241 gctatattgt ataccttgtt gctctttcat tctatgtaat aataacaaat tattatcatg
//
