LOCUS       c00213_gnlPro..         4254 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ONJOAAGF_213.
ACCESSION   c00213_gnlPro..
VERSION     c00213_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:45:54
            Original ID  :: gnl|Prokka|ONJOAAGF_213
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4254
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4254
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[228:3220]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      229..3220
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4254
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4254
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(229..981)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.9;
                     E-value: 2.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg213_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-33, bitscore:
                     103.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMAIVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTKENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKCHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIDPPTKERVENAKKILAI"
     CDS             complement(968..3220)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg213_2"
                     /sec_met_domain="Gly_radical (E-value: 2.5e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.8e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
ORIGIN
        1 gaaatatttt ggtccatgga gcaaagtaaa gaaaataaga ataaagtaaa ttccatagat
       61 catttgaaaa attagaattt aatttgagcg aattttaact gaagtttata tctaaagagc
      121 cccccatatt gtgacttcaa ggatagattg aagatgaata agtattcaac gcaatctatc
      181 ttcaaagtca caaatatggg ggtttttagg attctgtttg tcggcaaatc aaatagcaag
      241 aattttcttg gcgttctcca ctcgttcttt cgttggaggg tcaattcctt ctaaaggata
      301 ttctaatcca agttctttcc atttataaat acccatcgta tgataaggca ggatttccac
      361 cttttccaca ttatcaagag agtgaatgaa atcagctaat ctagtcaaat attcatcttt
      421 atcgcttcgt tccggaacaa ggacatggcg gatccaaatt ggttttttga tatcggaaag
      481 atagcgaatc atatcaagaa tattctcgtt gctgcgacca gttaaaactt tgtggcattt
      541 attatcaatg tgtttgatat ccactaataa caggtcagta tatttcatca attcctgaaa
      601 tttagaaaag aaaggttcct ctcttgtaaa aggtcctccg gcagtatcaa taacggtgtg
      661 gattcctttt tcttttgcct ttttaaaaaa ctcaagcata aaatcaatct gtaaaagagg
      721 ctctccacca cttacggtaa taccgccgct atttttccag taagtacgat aacggagtgc
      781 ctgatttaac agttcatccg cacttctcca caccgcacca ttttcctttg tcattttcca
      841 ggtgtccgga ttgtgacaga actgacaccg caaaggacaa ccctgtaaaa aaacaataaa
      901 acgaactccc ggcccatcca ccgaaccaaa actctccgta gaatgaacaa tagccatcgc
      961 atttttatta cattctgtca tggcatgttc tggcaattac atctaactgc tgttcttttg
     1021 tcagagagat gaatttaact gcataaccgg aaacacggat tgtaaagttt gcatattctt
     1081 ctttttccgg atgttccata gcatcaatca gtttctcaac accaaataca tttacgttta
     1141 agtggtgagc accctgtgcg aagtagccgt ccataacatt tactaagtta ttgatacgtt
     1201 cttcttcgga atgaccgagt gcatccgggt tgattgtttg tgtattggaa ataccatcta
     1261 atgcccattc gtaaggaagt ttagctacag agtttaaaga agctaagaga ccgttctgct
     1321 ctgcaccata acttgggttt gctcctgggg aaagtggttc accggctttt cttccatctg
     1381 gaagggaacc tgttgcctta ccatatacta cgttggatgt gatagtaagg atagaagttg
     1441 taggttcaga atcacgataa gtatggaatt tctccagttt tttcatgaat gtctgaagta
     1501 accatacagc gatatcatcg gcgcggtcat catcatttcc gtatttaggg aagtcgccag
     1561 ttgtctcata atcaacaaca agtccctctt cattgcggac tgtttttact ttggcatatt
     1621 tgatcgcact taaggaatct actacatgtg agaatcctgc gatacctgta gcgaatgtac
     1681 gacgtacatt tgtatcgatt aaggccatca aagcatattc atagtaatat ttgtcatgca
     1741 tgtactggat gagatttaat gtatttacat ataaacctgc taaccagtcc atcatgatat
     1801 catatttgtg cattacttca tcatagtcaa gatattcgga agtaatcggt ttatattccg
     1861 gtccaacctg ctgtccggtc ttttcatcca caccaccatt gatagcgtaa aggagacatt
     1921 ttgcaaggtt agcgcgagca ccgaagaact gcatttcctt accagtctgt gtagcagaaa
     1981 cacagcagca gatggagtaa tcatcgcccc atactggacg cattacatca tcattttcat
     2041 actggattga acttgttgta atagaaatat gagctgcgta atctctaaat gcttttggaa
     2101 gacgctcaga gtaaagtact gtgaggtttg gctctgggga tggtcccata ttttctaatg
     2161 tgtggaggaa acggtagtca gtttttgtaa ccatggaacg accgtcctgt ccaagtccgc
     2221 caacttcaag tgttgcccat acagggtcac cggagaagag ctggttgtaa gctgggatac
     2281 gtccgaactt aaccatacgg aacttcatag tcatatggtc gattaattcc tgcgcttctt
     2341 tctctgtaag gattccctta tccatatcac gtttgatata aatgtcaagg aaagtagcga
     2401 tacgtccgat actcatagca gcaccgttct gtgtcttgat tgctgcaagg tatccgaaat
     2461 acgtccattg aacagcttcc tttgcgttag aagcaggttc ggaaatatca tagccatagc
     2521 tttcagccat aactttcata tcttttaatg ctttaatctg catggagatt tcttctctta
     2581 aacggataac gtcatctgtc attgttccgt caccgcagtt tgcgaaatct ttttgcttgt
     2641 cagcaactaa caggtcaata ccgtaaagag ctacacgacg gtagtcacca acgatacgtc
     2701 cacgtccgta agtatctgga agaccagtaa tgatcttatt gtgacgagct ttcttcattt
     2761 caggagtata agaatcaaat actgcctggt tatgtgtctt atgataatct gtaaaaatct
     2821 tgtggaattc tggatttggt gtgtaaccat aagttgtaca agcttcttca gcagtacgga
     2881 taccaccaaa tggcataaat gcacgtttta aaggtttatc tgtctgaaga cctacgatct
     2941 gttctttctc ttttaaagac tcagaaatgt aacccggttt atgggaagta aggcttgata
     3001 cgatatcagt atccatatca agaacaccac ccttttcacg ctctgctttc tgcagcttct
     3061 gcagttcgcc ccacagtgtg tcagtagctt cggtaggacc ttcgaggaaa gaagcatctc
     3121 catcatatgg agtgtagtta tcctggatga actctcttaa attaatttct tctctccaga
     3181 ttcgtccatt aaatccctgc cattgttctt tttgtaacat cttaattgac ctccctgttt
     3241 gttaaaaaaa tccttaagtc aaggttttga aatatgtttg ctaaaaaatc aacaaaattg
     3301 ttcagatgat ttactatgat tagttttaac ttatttaagt tttaatcatc taacaacatt
     3361 attaacatga tttttggcaa tcgtcaaaca attttttgat attatgtgtt cttttttttg
     3421 aacatataca caaaaatgct tgtagagcct ttaaaataaa gggttttaga gcgttgataa
     3481 aatattatca aatcgtacaa atgtactgat ataagtacaa taatgacaag catacaacta
     3541 taattttatt tttcgctctt tagagcatgt ttggaaaaac atcttcttta gcacatgact
     3601 taataacgat ttaaagttac ttcttatcag ggatattgga tagaatatta tcttatcaga
     3661 aataaaaaag ccagcatcca gacaagaatt gcccagacgg tcggctataa gagtcttata
     3721 cttcatgtgg ttcattccac ttacttccgt cagtcgtata agtatcctaa ctataaacaa
     3781 aatagaacaa aatgtcaagg ggttactatt taattcatta agcgaactac agacgaagcc
     3841 attgccccga ataaggcata tttgtgattt tagtcgcggt gtgttgaatg ttcgctctat
     3901 gcactcgcat ccaacacttg ctccgagacc acaaatatgc cttattcggg gcaatggctt
     3961 ctgtgctatg gctggagaag atgaattaaa cagagggagg ggatttgggg acagaaactg
     4021 atgtcttctg cttactcaag aatgactggg aacaggggga tgatttagct tttttaatgg
     4081 ttatctcagc aatatttcaa taatttcaga ataaaaagca gactgttgtt atcaatatca
     4141 attcattacc tcttctattt aaatacaaaa catctattca caatatttta agagcaagca
     4201 gaaaacttct accctctccc ccaaatcccc acaaccacta tttaattcaa acaa
//
