LOCUS       c00116_gnlPro..         8082 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MBCHICJG_116.
ACCESSION   c00116_gnlPro..
VERSION     c00116_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:20:16
            Original ID  :: gnl|Prokka|MBCHICJG_116
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8082
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8082
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3803:7404]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3804..7404
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8082
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8082
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..158
                     /locus_tag="ctg116_1"
                     /transl_table=11
                     /translation="EIDLENLRGTSDMKKIKDLKGRPLLLFINSWSVGMTLERNEGLVL
                     SEFGRA"
     CDS             444..3617
                     /locus_tag="ctg116_2"
                     /transl_table=11
                     /translation="MIKSYNLSQSFRRTAKRCMVVTLASVVIAGSVITATPASAYARSV
                     DEAATKASTDISTPETSKDAFNKYKKIDGINGNTILGADFSYYQQCLEWGKTYKNYMSQ
                     PVDDIFKYVEDQGINTITLKVAVNPSGDNSYLSLENAIKTLKAVKQSKADLKTNVVLFY
                     SDSITYANDQRLPYGWTADNAVEEAKEYTQEVIEKLKAENVVPTIITIGNEVNCNFLDI
                     GTAWDSFVAMASMSAIIKQNNIKVGLSLSKPDNPQWILEQFGYATVQYDYFGVNVYPDD
                     NTNAEVEELRKAAEEYAPEKQLIVSNVKYARENEHKTVNVYTQADSIYNLLDATISEKN
                     AGGLIYDEAANAGNWNSLFDDEGDAQVSLSIFAYASTADDADRQKIDTSRDPYKYGDDT
                     GLKQQKVTISKVKNMTDSTIRGIDISSYTALKNAGVKYYDNNGNKASLLKILSDNGVNY
                     IRIRIWNDPYNENGETYGGGANGVKAGLEIAKEAAKYNIKVLLGFHYSDFWADPSVQLL
                     PKAWQKDKNDQKRMCSNVYNFTKETIEQFKDAGADIGMVQVGNEISHGMMGVMHKNKSN
                     IWQEEEKSSVIDSYINAGAKAVRECAPKALVAIHLDTLNLGMYKDAMNAWERDKVDYDV
                     LGASSYAFWVGKNMAYNVRKAGEYVASRGKLFAVLETSWLNSQQDADGTVNMVNSTKDA
                     TYKVGPQGQADMLADLYNAILSNDNGLGAFYWEGAWIPVKSGWVNWNYNKEMANKYGTG
                     WAAENALGYYPKSKLYYNGNPVWGGDSWDNQTLFDSKGYPLDSLRFYKDAVSSNTKYSR
                     VIVCLCDKSNNVLEYRVVKVKEGETISYTLPDISGYTATKKTIHLSGTDEDAARISVVY
                     SKAVQPEQPEQQTISVKKSSYRLPYGTNYNFKKQVKTKGSLTFTSSNSSIVSVGRRSGV
                     LTVKKPGKATIKITLAASGKRKNTSRRITIYVVPKRQTIKVSAAKEKIKVVVKKDQKAS
                     GYQMVIARNSKFSKDKEVISKKRTKRVTYTVTKLSSKKTYYVKARAYKIIGNKKYFGPW
                     SKVKKIRVK"
     CDS             complement(3804..4556)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.1;
                     E-value: 2.2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg116_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.6e-32, bitscore:
                     101.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKNTMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNPDT
                     WKMTDENGAVWKSADELLNQALRYRAYWKNGGGITVSGGEPLLQIDFLLEFFKKAKEKG
                     IHTVIDTAGGPFTREEPFFSKFQELIKYTDLLLVDIKHIDTECHKILTGRSNENILDMI
                     RYLSDIKKPIWIRHVLVPERSDKDEYLTRLSDFIHTLDNVEKVEILPYHTMGIYKWKEL
                     GLEYPLEGIEPPTKERVENAKQILDIVI"
     CDS             complement(5152..7404)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg116_4"
                     /sec_met_domain="Gly_radical (E-value: 5e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.4e-267, bitscore:
                     876.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLECPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
ORIGIN
        1 cagagattga ccttgaaaat cttcgtggaa catcggatat gaagaagatt aaggacttaa
       61 agggacgccc attgttactg tttatcaata gctggagtgt gggaatgacc ttagaaagga
      121 atgaagggct cgtactctca gagtttggac gagcataaga agacaattta attcgttaac
      181 aaacagacgc ccgacaggca agaaactatt tctcagatgt gtctttcgtc actgcagttt
      241 gaatgtccgc ttatcgagtt cgcatccaac ttttgttcca ggtcacatct gaggagtagt
      301 tttttgcctg tcgagctgtt tttagctggt gtagatgaat gaaattggag aaagaaaact
      361 ttaaatgata atttttttgc atgtattgag aaaagtgagg tattatgata aaatataatt
      421 tgatactata aaggaggaat attatgataa aaagttacaa tttaagtcaa tcattcaggc
      481 gaacagccaa gcgctgtatg gtagtaactc tcgcgtcagt ggttatagca ggttctgtga
      541 tcacggctac acctgcttct gcatatgcca gatcggtaga tgaggctgca acgaaagctt
      601 ccactgatat cagtacccct gagacaagta aagatgcttt taataagtat aagaaaattg
      661 atggaatcaa tggcaacact attttaggag cggacttttc ttattatcag cagtgcctgg
      721 aatggggaaa aacgtataaa aattatatga gccagcccgt tgatgatatt tttaagtatg
      781 tagaagatca gggaattaat acgattactt taaaagttgc ggtgaatcct tccggagata
      841 actcatatct ttctctggag aatgcgatta agacattaaa agctgtaaag cagtcaaaag
      901 ctgatctcaa gacgaatgtc gtattatttt attctgacag tataacttat gcgaatgatc
      961 agaggcttcc gtatggatgg acagcagata atgcagttga ggaagcaaaa gagtataccc
     1021 aagaagtgat agagaaactc aaagcggaga atgtagttcc aacaattatc acgattggaa
     1081 atgaagtgaa ttgcaacttt ctggatatcg gtactgcatg ggactcattt gttgcgatgg
     1141 cttctatgtc tgcaattata aaacagaata atattaaagt agggcttagt ttatcaaaac
     1201 cggacaatcc tcagtggatt ttagaacaat ttggatatgc aacagtacag tatgattatt
     1261 ttggtgtgaa tgtttatccg gatgataaca cgaatgcaga agttgaagaa ttaagaaaag
     1321 cagcagaaga gtatgcaccg gaaaagcagc ttatagtttc taatgtaaag tatgctcgtg
     1381 agaatgagca taaaactgtg aatgtgtata cgcaggcaga tagtatttat aatttattag
     1441 atgcaaccat cagcgaaaag aatgcaggtg gcttaattta tgatgaagca gccaatgcag
     1501 gaaactggaa ttctttgttt gatgatgagg gcgatgctca ggtttctctt tctatttttg
     1561 catatgccag tacagcagat gatgcggaca gacagaagat cgatacaagc agggatcctt
     1621 ataaatatgg ggatgatact ggattaaaac agcagaaagt aacaattagt aaggttaaaa
     1681 atatgacgga ttctacaatc cgtggtatag acatttcctc ttatactgct ctgaagaatg
     1741 caggagtaaa gtattatgac aataatggaa acaaagcttc cttgttgaag attctcagcg
     1801 ataatggagt aaactatatt cgtatccgta tctggaacga cccttataat gagaatgggg
     1861 aaacttatgg cggaggagcg aatggtgtta aggcaggatt agagatagca aaagaagcgg
     1921 caaagtataa catcaaagtt ttgttaggat tccactattc tgacttctgg gcagatcctt
     1981 ccgttcagtt gttaccaaaa gcatggcaaa aagataaaaa tgatcagaag agaatgtgct
     2041 ctaatgtata taattttacg aaagagacga tagagcagtt caaagatgcc ggtgcagata
     2101 tcggcatggt acaagttgga aatgaaatct ctcacggtat gatgggagtt atgcataaga
     2161 ataagtctaa tatatggcag gaagaagaga aatcttctgt tattgacagt tatataaatg
     2221 caggagctaa ggctgtcaga gaatgtgcac caaaagcatt agtagcgata catctggata
     2281 ctctgaatct gggaatgtat aaggatgcca tgaatgcctg ggaaagagat aaagtagact
     2341 atgatgttct cggtgcatcc agttacgctt tttgggttgg caaaaatatg gcttataatg
     2401 ttcgaaaagc tggagaatat gttgcatcca gaggtaagct gtttgcagta ttagaaacat
     2461 catggcttaa ctctcagcag gatgctgacg gaacggtaaa tatggtaaac agcacgaagg
     2521 atgctactta taaagtgggt cctcaaggac aggcagatat gttagctgac ctgtacaatg
     2581 caatattatc caatgataat ggattaggtg cgttctattg ggaaggcgca tggattccgg
     2641 ttaagtctgg ttgggtaaac tggaattata ataaggaaat ggccaataaa tatggaacag
     2701 gctgggcagc agaaaatgca ttgggatatt atccaaagag caagttgtac tataatggaa
     2761 atccggtttg gggcggagat tcatgggata atcagactct ttttgacagc aaaggttacc
     2821 cattagattc tcttcgtttt tataaagatg ctgtatcttc caatacaaaa tattcacgag
     2881 tgatcgtttg tttatgtgac aaatcaaata atgtactcga atatagagtt gtaaaggtga
     2941 aagagggaga gaccatctct tatacattac cagatatcag tggatataca gcaacgaaaa
     3001 agacaatcca tctttcagga acagacgaag atgccgcacg tatatctgta gtttatagta
     3061 aggctgtaca accagagcaa ccagagcagc agacgatttc tgttaagaag tcttcttata
     3121 gactgccata tggcacaaac tataatttta agaagcaggt aaaaacaaaa ggaagcctga
     3181 catttacttc aagtaattcc agcattgtct cagtcggaag acgaagtggt gtgcttacgg
     3241 tgaaaaaacc aggtaaagca acgattaaga taacgttagc tgcatcagga aagcgcaaaa
     3301 atacaagcag aagaattacc atttatgtag tgccaaaacg tcagacgata aaagtttccg
     3361 cagcgaaaga aaagatcaaa gttgttgtga agaaggatca gaaagccagc ggatatcaga
     3421 tggttattgc cagaaattcg aagtttagca aagataaaga agtaatcagc aagaagagaa
     3481 caaagcgtgt aacatacact gtaacaaagc taagttcgaa aaagacatat tatgtgaagg
     3541 cacgtgctta taagataatc ggtaataaaa aatactttgg tccatggagt aaagtaaaga
     3601 aaataagagt aaaataagac gatatcaaca gtcagagcgt gtagttcaac aaaaaggaaa
     3661 atagggaaaa gccagtatat aaaaatgtat ctaaatgcat ctgtaagcaa tctcttaatg
     3721 tgagcacttt gagcgagcag taaggatgca atgaaataaa gcatttttat atatggactt
     3781 tttccctatt ttttcttttt ggactaaatt acgatatcta atatttgctt tgcgttctcc
     3841 actcgttctt ttgttggcgg ctcaattccc tccagtggat actccaatcc aagctctttc
     3901 catttataaa ttcccattgt gtgataaggc agaatctcta ctttttctac gttatcaaga
     3961 gtatgaatga agtcagataa tctggttaaa tattcatctt tatcgcttcg ttccggaaca
     4021 aggacatggc ggatccaaat tggtttttta atatcagaaa gatagcggat catatcgaga
     4081 atattttcat tgctccgacc agttaatatt ttgtggcatt cggtatcaat gtgcttgata
     4141 tcgaccagga gcaagtcggt gtattttatc aattcctgaa acttggaaaa gaaaggttct
     4201 tctctggtaa agggtcctcc ggcagtatca ataacggtat gaattccttt ttcttttgct
     4261 tttttgaaaa attccagaag aaaatcaatc tgcaataaag gttccccgcc gctgacagta
     4321 atgcctccgc catttttcca gtaagcacga tagcggagtg cttgatttaa cagctcatct
     4381 gcgcttttcc ataccgcacc attttcatct gtcatcttcc atgtatctgg attgtgacag
     4441 aactgacacc gcaaagggca accctgcaaa aaaacaataa aacgaactcc cggcccatct
     4501 accgaaccaa aactttctgt agaatgaaca agagccatcg tatttttatc actcataaat
     4561 aacacctcat aatattttca gtataaaaat aaattactag agcgtgtttg aaaaatgctt
     4621 tctgcaagat gtgcgttaca atttgtggaa gattttacct gaataaaggc ggcgtagtgg
     4681 gctacgtcaa ctgaatgaag gcaaaatctt ccgcaaattg gggcgtgcag attgcaggaa
     4741 tgatttttca aacacgctct aagaagaaat tagtatattc ataataaata gatatatatg
     4801 aataattgaa aatagcaatt aaaagatata attatttttc tacttccgct atagccccaa
     4861 ataagttatt tgcagtaaaa aatactatta caacagcatt ttttactgca aatgttttac
     4921 ttaaaatcag gatgtcgaca agctatatgg taaagaacgt aatacaaaaa gagaatactt
     4981 ttatcattcc atactatgga attttgactg tagccgctcg aactgctttc ttcgaagcgg
     5041 ctgttttatc aggcaaaatg aaatgtacag tacggtgtat aaaagtatct ctttttgtat
     5101 tacgctcttt tcaaaaaact agagtcagca tccgtaagga taaaacattt actacattct
     5161 atcgtggcat gttctggcaa ttacatctaa ctgctgttct tttgtcagag agatgaattt
     5221 aactgcgtaa ccagaaacac ggattgtaaa gtttgcgtat tcttcttttt ctggatgttc
     5281 catagcatca atcagtttct caacaccaaa tacatttacg tttaggtggt gagcaccctg
     5341 tgcgaagtag ccgtccataa catttactaa gttattgata cgttcttctt cggaatgacc
     5401 gagtgcatca gggttgattg tctgtgtatt ggaaatacca tctaatgccc attcataagg
     5461 aagtttagct acagagttta aggaagctaa aagtccgttc tgctcagcac cataacttgg
     5521 gtttgctcct ggggaaagag gttctccggc ctttcttccg tctggaaggg aacctgttgc
     5581 cttaccatat actacgtttg atgtgatagt aaggatggaa gttgtaggtt cagaatcacg
     5641 gtaagtatgg aatttttcaa gcttcttcat aaatgtctga agtaaccata cagcgatatc
     5701 atcagcgcgg tcatcatcat ttccgtattt agggaagtcg ccagttgtct cataatcaac
     5761 aacaagtcct tcttcattgc ggactgtttt tactttggca tatttaatcg cacttaagga
     5821 atctactaca tgtgagaatc ctgcgatacc tgtagcgaat gtacgacgta catttgtatc
     5881 gattaaggcc atcaaagcat attcatagta atatttgtca tgcatgtact ggatgagatt
     5941 taatgtattt acatataaac ctgctaacca gtccatcatg atatcatatt tgtgcattac
     6001 ttcatcatag tcaagatatt cggaagtaat cggtttatat tccggtccaa cctgctgtcc
     6061 ggtcttttca tccacaccac cattgatagc gtaaaggaga cattttgcaa ggttagcgcg
     6121 agcaccgaag aactgcattt ccttaccagt ctgtgtagca gaaacacagc agcagatgga
     6181 gtaatcatcg ccccatactg gacgcattac atcatcattt tcatactgga tagaacttgt
     6241 tgtaatggaa atatgagctg cgtaatctct aaatgctttt ggaagacgct cagagtaaag
     6301 tacggtgagg tttggctctg gggatggtcc catattttct aatgtgtgga ggaaacggta
     6361 gtcagttttt gtaaccatgg aacgaccgtc ctgtccaagt ccgccaactt caagtgttgc
     6421 ccatacaggg tcaccggaga agagctggtt gtaagctggg atacgtccga acttaaccat
     6481 acggaacttc atcgtcatat ggtcgattaa ttcctgcgct tctttctctg taaggattcc
     6541 tttatccata tcacgtttga tataaatatc aaggaaagta gcgatacgtc cgatactcat
     6601 agcagcaccg ttctgtgtct tgattgctgc aaggtatccg aaatacgtcc attgaacagc
     6661 ttcctttgcg ttagaagcag gttcggaaat atcatagcca tagctttcag ccataacttt
     6721 catatctttt aatgctttaa tctgcatgga gatctcttct cttaaacgga taacgtcatc
     6781 tgtcattgtt ccgtcaccgc agtttgcaaa atctttctgc ttgtcagcaa ctaacaggtc
     6841 aataccgtaa agagctacac gacggtagtc accaacgata cgtccacgtc cgtaagtatc
     6901 tggaagacca gtaatgatct tattgtgacg agctttcttc atttcaggag tataagaatc
     6961 aaatactgcc tggttatgtg tcttatgata atctgtaaaa atcttgtgga attctggatt
     7021 tggtgtgtaa ccataagttg tacaagcttc ttcagcagta cggataccac caaatggcat
     7081 aaatgcacgt tttaaaggtt tatctgtctg aagacctacg atctgttctt tctcttttaa
     7141 agactcagaa atgtaacccg gtttatggga agtaaggctt gatacgatat cagtatccat
     7201 atcaagaaca ccaccctttt cacgctctgc tttctgcagc ttctgcagtt cgccccacag
     7261 tgtgtcagta gcttcggtag gacattcgag gaaagaagca tctccatcat atggagtgta
     7321 gttatcctgg atgaactctc ttaaattaat ttcttctctc cagattcgtc cattaaatcc
     7381 ctgccattgt tctttttgta acatcttaat tgacctccct gtttgttaaa aaaatcctta
     7441 agtcaaggtt ttgaaatatg tttgctaaaa aatcaacaaa attgttcaga tgatttacta
     7501 tgattagttt taacttattt aagttttaat catctaacaa cattattaac atgatttttg
     7561 gcaatcgtca aacaattttt tgatattatg tgttcttttt tttgaacata tacacaaaaa
     7621 tgcttgtaga gcctttaaaa taaagggttt tagagcgttg ataaaatatt atcaaatcgt
     7681 acaaatgtac tgatataagt acaataatga caagcataca actataattt tatttttcgc
     7741 tctttagaac atgtttggaa aaacatcttc tttagcacat gacttaataa cgatttaaag
     7801 ttacttctta tcagggatat tggatagaat attatcttat cagaaataaa aagccagcat
     7861 ccagacaaga attgcccaga cggtcggcta caagagtctt atacttcatg tggttcattc
     7921 cacttacttc cgtcagtcgt ataagtaccc taactataaa caaaacagaa caaaatgtca
     7981 aggggttact atttaattca ttaagagaca gacgcccggg aagagaacga taccctatat
     8041 ttgtgacttt agtcgctgcg gtttaaatgc tcgctttatg cg
//
