LOCUS       c00466_gnlPro..         3591 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PPJFMCDP_466.
ACCESSION   c00466_gnlPro..
VERSION     c00466_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:17:55
            Original ID  :: gnl|Prokka|PPJFMCDP_466
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3591
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3591
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2945]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2945
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3591
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3591
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..2203
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg466_1"
                     /sec_met_domain="Gly_radical (E-value: 2.5e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEAL
                     AEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSK
                     ACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIG
                     DYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLD
                     LSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQ
                     ELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLY
                     NLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCV
                     SYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNY
                     KKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSA
                     IKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNA
                     RPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRY
                     RDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAME
                     HPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2217..2945
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.4;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg466_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-31, bitscore:
                     96.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRLEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2938..3387)
                     /locus_tag="ctg466_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3374..3541)
                     /locus_tag="ctg466_4"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
ORIGIN
        1 cggtctttgg agcagcgaaa tcaacgtcag agatttcgta agtcataaca tcactccgta
       61 ttacggagat gcttcattcc tcgaaggacc tacagaacgt accaaagccg tatggaaccg
      121 ctgcctcgaa gcgttggcag aagaaagaga aaacaacggt gtccgctcat tagataatgt
      181 taccgtatca accatcactt cccacaaggc cggatatatc gataaggaaa acgaactgat
      241 cgtcggtctg cagacagacg aacttctgaa acgtgccatc aagcctttcg gaggtatcaa
      301 cgtagtcagc aaagcctgtc acgaaaacgg tgtggaagtg gacgaccgcg taaaagatat
      361 tttcactcac taccgcaaga cgcacaacga cggagtattc gacgtatata cggaagaaat
      421 ccgttccttc cgttcgctgg gattcctcac cggacttccc gacaactatg cacgcggacg
      481 tatcatcggt gactaccgcc gcatggcact ttacggcatc gaccgtctga tcgaagcaaa
      541 gaaggaagat ttgcacaacc tcaccggtcc gatgacagag gcccgcatcc gtctgcgcga
      601 agaagtggca gaacagatca aggcactgaa agacatgaaa gtgatgggcg aatactacgg
      661 tctcgacctg agccgtcccg cttacacggc acaagaagcc gtacagtggg tatatatggc
      721 ttaccttgcc gccgtcaaag aacaagacgg tgccgctatg tcactgggta acgtttcttc
      781 tttcctcgat atctatctgg aatatgaact gagcaaagga accatcaccg aatcgttcgc
      841 gcaagagctg atcgaccagt ttgtcatcaa actgcgtatg gtccgccacc tgcgtatgca
      901 atcatacaac gacatctttg caggcgaccc gacctgggta accgaatctc tgggcggacg
      961 tctcaacgac ggacgtacca aagtgacaaa gacctccttc cgtttcctgc agacattgta
     1021 caacctcggc ccttcaccgg aaccgaacct gaccgtactc tggagcccgg aacttccgga
     1081 aggattcaaa gaattctgtg caaaggtttc tatcgacact tcttctatcc agtacgaaaa
     1141 cgacgacctg atgcgcgaag tacgtcagtc cgacgactac ggaatcgcct gctgcgtatc
     1201 ataccaggaa ataggtaagc agattcagtt cttcggtgcc cgttgcaacc tggccaaagc
     1261 cctgttgctt gccatcaacg gcggacgttg cgagaatacc ggtacagtca tggtgaagaa
     1321 catccccgtg ctgactagcg acacgctgaa gttcgaagaa gtaatggaca actacaagaa
     1381 agtgttgacc gaaatcgccc gcgtatacaa cgaagcgatg aacatcatcc actatatgca
     1441 cgacaagtat tactacgaga aagcccagat ggctcttgta gatacgaacc cgcgcataaa
     1501 ccttgcttac ggtgttgccg gactttccat cgcactcgac tcactgtctg ccatcaaata
     1561 tgcgaaagta actgcccgcc gcaacgatat cggcctgaca gaaggcttcg acatcgaagg
     1621 agaattccct tgcttcggta acgacaacga caaggtagac caccttggcg tagacctggt
     1681 atacttcttc agcgaagaat tgaagaaatt gcctgtttac aagaatgccc gtcctaccct
     1741 ctccttgctg actatcactt ccaatgtgat gtacggcaaa aagaccggtg ctactcccga
     1801 cggacgtgcc aaaggtgttg ccttcgctcc gggtgccaac ccgatgcacg gacgcgacaa
     1861 gaatggtgcg atcgcttctc tgagttctgt agcgaaactt cgttaccgtg actcacagga
     1921 cggtatcagc aacaccttct ctatcgttcc gaaatcactg ggtgctacgg acgaagaccg
     1981 tatcgagaac ctcgtgacga tgatggacgg ttacttcacc aaaggtgccc accacctgaa
     2041 cgtaaacgta ctgaaccgtg atatgctgta cgatgccatg gagcatccgg agaactatcc
     2101 gcagctgacc atccgcgttt ccggctatgc cgtaaacttc gtgaagctga gccgcgaaca
     2161 ccaactggaa gttatcagcc gtagcttcca cgaacgtatg taatctatta ctctatatga
     2221 tgataaacgt acattcatac gaaagtatgg gaacattcga cgggccgggc ttacggctcg
     2281 tcgtttttct tcaaggatgc aacttccgct gcctctattg cgccaatccg gatacgatag
     2341 caggaaaggg aggcacgcct accccaccgg aagaaatcgt ccgcatggca atgagccagc
     2401 gccccttctt cgggaaacga ggaggaatca ctttctccgg aggcgaaccg acgtttcagg
     2461 cgaaagcact cgtcccgctg gtccgcgaac tgaaagaaaa aggcatccat gtctgtctgg
     2521 acagcaacgg aggactttgg aacgaagacg tagaagaatt attcagactg accgatcttg
     2581 tgttattgga tataaaggaa ttcaacccca accgccatca gacgctgacc ggaagaagca
     2641 acgagcaaac catccgcacg gccgcctggc tggaagaaca gggaaaacct ttctggctgc
     2701 gttatgtgct agtgcccgga tacagcgatt ttgaagaaga catacgcgca ctgggagaag
     2761 cactcggaaa atacaagatg atccaacggt tggaaatact gccttaccat acactgggcg
     2821 tacataagta cgaagcgatg ggacaggaat acaagatgaa aggggtaaaa gaaaataccc
     2881 ccgaacagct tgaaaaagct gcggaggtat tcaaagagta tttcactacg gtagtagtta
     2941 attaatggag aattctccaa tgacctcaaa gcaagggcat tctttaatcc gttcccaagg
     3001 atctacgatc ccattatagt tggtgtcggg acttaaatcc cgatgcccta ctactttagc
     3061 ttccggataa cgctgcaaca attcgtgtac caagcgatgt agcgcctcct tctgacgggg
     3121 tgtacgcgta tccgccgctt ttccattctc atccagaccg ccctcatagc atacacctaa
     3181 gctgatcgca ttgtagccac gtgcatgagc tccgaccgta tcttcatccc gcatcggctc
     3241 aatctcgccc gacttgcgta tataataatg atacccccag cacgaaaagc cacgataacg
     3301 gtgagccgta tccacatcct ttgccgtaaa gtcgcaatcg acacgggtgg cggaacaatg
     3361 gatcacaatc agactaatct ttctcatgtt ccttttccct ttccgtcgtt ttcactttgc
     3421 acacctcctt acaagtagta tgatagcgca cttctctcac caccttgtcc cgctttctct
     3481 ttccgaagaa aggaatcaga tacatgatca catctaaaat ccggtttact atttctttca
     3541 tcttatcaaa atattaagtg gtagttattt aaattcgtta ttaccttgac c
//
