LOCUS       c00055_gnlPro..        15199 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FPGOFBJB_55.
ACCESSION   c00055_gnlPro..
VERSION     c00055_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:55:37
            Original ID  :: gnl|Prokka|FPGOFBJB_55
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 1958
            Orig. end    :: 17157
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15199
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[11958:17157]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..15199
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..15199
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15199
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             complement(258..389)
                     /locus_tag="ctg55_4"
                     /transl_table=11
                     /translation="VKTYIEERAMEIARYIIDNNTTVRQAAKHFGISKSTVHIDVTM"
     CDS             complement(458..754)
                     /locus_tag="ctg55_5"
                     /transl_table=11
                     /translation="MKDNRNRYNEEIEKYQSMDQFQVGIAVFDPDTTETQFYDNVRSVC
                     EMMNNNISISVGVTWAEHGKEFNEKIMEADWRMYEQKNAYHQQQQAAQQELLQ"
     CDS             complement(757..1026)
                     /locus_tag="ctg55_6"
                     /transl_table=11
                     /translation="MCRADLTERAAEEKDMLVLAKEEENIVVNKPALSQIRSICELATL
                     ECYYHNVEKSAKTKGTGLAHVGEKEKNSGSNTPELLRLKSTCRM"
     CDS             complement(1242..2903)
                     /locus_tag="ctg55_7"
                     /transl_table=11
                     /translation="VLTNGETFSYDKNEIESYVVTGLKYVPVKVKTEDYEAFKAAYTVV
                     ENGSTLSGGFSEGNLKNYTDLVAEVTENTNGLKTVTQNEDGSFSFAARVNNGTDSGIKD
                     AALKTAENITTTVKEANGSYGEFLRVDLTGEGYGALGADMQAAEWTYYGSDSTYTDPLQ
                     SYGTKFASDNWMHKAQGIQLGLTDSLRCKLPAGTDGTGYWTITVYALGYNDYTVKFKVT
                     DANIVKDEEETVDTTALETAIKSAENLTESDYMAASWSDLCVELKEAKDELAAPHTQST
                     VDEATEHLDAAIKALVKAETKEETKTDVTKLNAVIEKAEALKQSDYTAESWKNLQTALD
                     AAKKLTDATAEQTVVDQAASDLETAILALVKADTENTGTTGKKKKPAVGTVKTVGQIKY
                     KVTGKNTVTVNKYAMKNITKASIPATVKINGYTFKVTAIADSAFSGCSKLTKVTVGSNV
                     KAIGNKSFYKCTKLTTFTASSTGLNKIGKEAFSGDKKLANITLKTTKLKKSGVGKDAFK
                     NIKKNATFKVPAKKVSDYKAIFKSKGAGKNIKVKKL"
     CDS             complement(2897..3244)
                     /locus_tag="ctg55_8"
                     /transl_table=11
                     /translation="MKRKNVSARILTGVLAFSMVAATGTSSMTVFAAEESAVEAEQITG
                     TEVKSETGETQEYQYAYAALSLTRVHLIPYREQPPITDCTGEVISVRQKLQKKMVQSSK
                     WHIIQEQPPVC"
     CDS             complement(3396..4175)
                     /locus_tag="ctg55_9"
                     /transl_table=11
                     /translation="LEGTKLKNIVEIIQKNCISKERKTNGAVFWKKHKKQIPAWILAGI
                     QLLFFVFFPSAFTAAFSGVKYIAGQMGTGAQIEITSFVLSLIILCASTVVFGRFFCGFA
                     CAFGSLSDWVRAAYVWVCKKLKKKPVTLGMELTAAVRGMSFPCFMQEILHWKDILRESS
                     SSFSSCVACACRSVFSAVFCARWERYFHFFQYCHILRSGEKGRTVSLAVQHARKNVPRI
                     SDCRNWGRLRREATVLCVRNVPVSVRKEISTVVSLEN"
     CDS             complement(4190..4627)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FMN_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_10"
                     /sec_met_domain="FMN_bind (E-value: 4.6e-17, bitscore:
                     52.9, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDFLIRSFCLAAVILILAGYNQVLKDRSKDEEITKLEAQVTKLQ
                     QEKEKTVDIKGTYPDGRWEGRAKGFGGMITVLVTVENGTISEIEITSADGEDKAYLSMA
                     EDIIPKIIEAQSADVDTVSGATFSSTGIRDAVSEALKQAEQ"
     CDS             complement(4641..6323)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FMN_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_11"
                     /sec_met_domain="FMN_bind (E-value: 7e-18, bitscore: 55.5,
                     seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNISGIVPVILAAVVGIGAVVSLSDYTAPVYGSESEDELTETET
                     IIESAGMESVEETEVENTEAAVVLDGAFDLADGTYEGSANGFSGKIKVSVVIKNQTIRS
                     INILSNSDDEAFFNRAKEGVTASIIAKQSTDVDTVSGATYSSRGIINAVKDALSSSDGE
                     EQTVVAKGDFALNDGYYEGTGNGFAGPVKLFIEIKDKSIVGIYIVKTSDDAGFFNRTKE
                     GVTASILEKQSTDVDTVSGATYSSRGIIEAVSNAMEAAKKAKKGETDTETEDDTEKPDT
                     SKASSYYGVVVCNPDESGAFTAYNLHVKVMFQNGKIIAVSDVYGDGDSQNDTYITKAVS
                     GSSKHPGVVTQIVNKNGIDGVDTVSGATCTSKAVLHAVDVVVKAAAKDGNYTFSGSQSG
                     DDDTKNSEDTEKDSEDTENNSENTENNSEDTENRGENTESGGDTEKNDTENTENSGETT
                     LIYKNGDYKVSVVCIPDEDEDFDEYKLTLTLVIENDQIKEIKDISGDGGKSNSFYIKAA
                     SKGVVSQILQKNSTEGIDTVSSATCSSKAIIEACQKALEQAKN"
     CDS             complement(6350..7390)
                     /locus_tag="ctg55_12"
                     /transl_table=11
                     /translation="MSEGKVKRKIIFLIFAAVMVVAGGCGTDGGDTEASRDLFAMDTYM
                     TVTAYGKGATEAVDQAADEISRLDQLLSTGNEDSEIYKINQSGDGILSEETAYLVERSL
                     DLYEATDGAFDIAIYPVMKAWGFTDDNFRVPDADELKDLLTLTDASDISYDKETSQIAF
                     KKDGMQIDFGGIAKGYTSARIMDIFRACGIKSGLVNLGGNVQALGTKKDGSSWRVAVQD
                     PQDTDQYLGVLSIQDKAVITSGGYERYFEQDGRTYHHIIDPKTGYPVENGLISVSIVTA
                     DGTLADGLSTSVFIMGKEAATEYWRNHSDEFDMILMTDDREIYVTEGIADSFESEMDTK
                     IIEKKV"
     CDS             complement(7586..8269)
                     /locus_tag="ctg55_13"
                     /transl_table=11
                     /translation="LRNEHASVILNNVVKLHITRKEEETMTQKKFSTKYLVEMALLVAI
                     ILIMAFTPIGYIRTAGLEITLIVVPVAVGAVTLGPAAGAILGGVFGVTSFIQCFGMSPF
                     GAALLGINGFLTFLVCVPTRILMGWLTGLIYKGLRKTKLPSGVSVTISNLCCPLLNTTF
                     FMGMLVIGFYNTEYIQSFVSALGAKNALLFILAFVGVNGLVEAIVCFVVGTAISAALKK
                     ALKYN"
     CDS             complement(8340..8948)
                     /locus_tag="ctg55_14"
                     /transl_table=11
                     /translation="MNKKKFSTKFLVEMALLVAIILIMAFTPLGYIKTAGIEITLIVVP
                     VAVGAVTLGPAAGAILGGVFGLASFLRCFGLNAFGAMLLSINPFLAFLLYVPTRILTGW
                     LTGLIYQGIRKTKIPSNVSITIANLCCPLLNTTFFMSMLVFGFYQTEYIQSFVQTLGVS
                     NPFLFVIAFVGINGLVEAVVCFIVGTAISAALKKALKYS"
     CDS             9344..9646
                     /locus_tag="ctg55_15"
                     /transl_table=11
                     /translation="MTPPPITALASDILPTTGNVRALVVVVDFADAKYDTDRRLSDEEI
                     SSYLFGTDNTEFYPCESLTSYFDRASYGSLHMTGNVFHYTAKGTIASYEKTADGS"
     CDS             complement(10001..11047)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 161.3;
                     E-value: 7.7e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_16"
                     /sec_met_domain="ETF (E-value: 2.5e-50, bitscore: 161.3,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 1.1e-38, bitscore:
                     121.7, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALEEYKGVFVFAQQVDNVLDGVAFELLGKGKDLAKDLGTDVTAV
                     LIGSGVKGLADQLAEYGADKVIVVDDPELKDYRTEPYAHALASVINEFKPEIMLVGATA
                     IGRDLGPTVSARVKTGLTADCTSLEIGDFPLVAAPGKESEQKHNQLLMTRPAFGGNTIA
                     TIACPDNRPQMATVRPGVMQKIAPISGAKANVVEYNPGFTPNNRYVEILNIVKAVKSTA
                     NIMEAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQTGKT
                     VRPQIYFAIGISGAIQHVAGMEDSDLIIAINKDEDAPIFDVADYGLVGDLNKIVPALTT
                     ALKAELGK"
     CDS             complement(11074..11856)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 159.1;
                     E-value: 3.7e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_17"
                     /sec_met_domain="ETF (E-value: 1.2e-49, bitscore: 159.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLAIMNPDDKAGLEAA
                     LRIKDETGAKVTVLTMGLPKADAVLREALAMGADEAVLVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAADIKVEGDSVVVKRQYEDRY
                     HELKAKMPCLVTALSELNEPRYMTPGGIFDAFDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVAKGAGEKVNLDPAESVAYLIGKFKEKHII"
     CDS             complement(11913..13067)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_18"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.1e-52, bitscore:
                     168.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.1e-31, bitscore:
                     97.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 7.8e-40, bitscore:
                     126.6, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFKEFAETEVKPLAQETDETEAFPAETVKKM
                     QKYGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVVVSAHTSLCIDPIMTYGTE
                     EQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSITEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGPEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRSIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             complement(13097..13969)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_19"
                     /sec_met_domain="HCDH (E-value: 3.3e-35, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 2.2e-63, bitscore: 203.8,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYEVALCDIKQEWAEGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFENMEVKKTTFKELDE
                     IAKPECIFASNTSSLSITEIGNGLNRPMVGMHFFNPADRMKLIEVIAGVNTPDETVAAI
                     KKISEEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             complement(14063..15199)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_20"
                     /sec_met_domain="Thiolase_N (E-value: 1.2e-93, bitscore:
                     303.6, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 2.6e-51, bitscore:
                     162.9, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GKMGGALSNTPAADLGAIVIKEALRRANVKPEMVDEVKMGCVIQA
                     AQGQNVARQAAIKAGLPIEVPAITINVVCGSGLKCVNDAATMIMAGEADIVVAGGMENM
                     SMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITAENVCDKYGITREELDE
                     FSANSQQKCEKAIAEGKFDDEIVPVPVKVKKEIVDFVKDEGPRAGTTAESLAKLKCCSG
                     KEGGLVTAGNASGINDGAAAIVVMSEEKAKELGVTPMATWVAGALGGVEPEIMGVGPVA
                     STRKVLAKTGLSIDDMDLIEANEAFAAQSIAVARDLKFDMSKVNVNGGAIALGHPVGAS
                     GCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVER"
ORIGIN
        1 aaacaactac ttcctttccc cccaagacca atacttccat cgcaacactt atttcaaaat
       61 tgcaaaaaga atcatataat aatactgccg ttttcttcat acgttttcac cctcctcata
      121 ttttttactc attgtcttct aaaattcaca cttccatctc atacatatta tatcaaaatc
      181 actctcagat ttccacctga gagtgatcct actttatccc tattcaatca cccaaacaat
      241 cttaccttta aatcgttcta cattgttacg tctatatgta ccgtactctt gctaatccca
      301 aaatgtttcg ccgcctgacg caccgtcgta ttattatcaa taatatatct ggctatttcc
      361 atagctcttt cctcaatata ggttttcaca aaaactcccc tgcaatactt gtttttacat
      421 tgtatgcagg ggagttatat actattcttt caaacattta ttgtagcagt tcctgttgtg
      481 cagcctgctg ctgttgatga tatgcatttt tctgttcata catccgccag tcagcttcca
      541 tgatcttctc attaaattct tttccgtgct ctgcccaggt aactcccact gaaatgctaa
      601 tattgttgtt catcatttca cagacagatc ttacattatc ataaaattgc gtttctgtcg
      661 tatccggatc aaatactgca atgccaacct gaaactgatc catactctga tatttttcta
      721 tttcctcatt gtatctgtta cgattatctt tcatatttac atccgacatg tcgatttcag
      781 tcttagcaac tccggtgtat tcgatccaga atttttttct ttttctccca catgtgccaa
      841 tccggtacct tttgttttcg cggacttttc cacattgtga taataacatt ctagtgttgc
      901 aagttcacag atcgaccgga tctgtgacag ggcaggttta ttgacaacaa tattttcctc
      961 ttcctttgcc agcacaagca tatccttctc ttctgctgca cgctctgtca gatccgctct
     1021 gcacatttca tacgcttcct gcgatatcgt ttctgtttca tatttttatt ttcaaaaata
     1081 taatccatag aaccaaaacc taccgccatg ttctgtatct cactctccgg aagtgtgagg
     1141 gtgattgttt ttgtttcctc ctcaaaggtt caattacaca ttccattcta caataaggtc
     1201 tcttcacccc ctgtttttta acaaaagaat attttttcac cctacagttt ctttacctta
     1261 atattttttc cagcaccctt tgacttaaaa atagctttat aatcagaaac tttttttgcc
     1321 ggtactttaa aagttgcatt tttcttaatg ttcttgaatg catcctttcc aacacctgat
     1381 tttttcagtt ttgttgtctt taatgtgata tttgcaagtt ttttatcacc ggaaaacgct
     1441 tcctttccaa tcttgtttaa tccggtagaa gatgccgtaa atgtggtaag ttttgtacat
     1501 ttataaaatg atttatttcc gattgccttt acattagaac caaccgtaac tttcgtaagt
     1561 ttactgcatc cggaaaatgc gctgtctgca attgcagtta ccttaaatgt atatccgttg
     1621 attttaaccg ttgcaggaat agatgccttt gtaatgtttt tcattgcata tttatttaca
     1681 gttactgtat tttttccggt tactttatac ttgatctgtc caactgtttt cacagttcca
     1741 actgccggtt ttttcttttt gcctgtagtt ccggtatttt ccgtatcagc ttttaccagc
     1801 gccaaaatcg ctgtttccag atcacttgct gcctgatcta ctacagtctg ttctgctgtt
     1861 gcatctgtaa gtttctttgc agcatcgagc gcagtctgta aatttttcca gctctctgct
     1921 gtgtaatcac tctgctttaa tgcctccgct ttctcgatta cagcgttaag ttttgtaaca
     1981 tctgtttttg tttcttcctt tgtctctgcc tttaccagtg ctttgattgc agcatccaga
     2041 tgctctgttg cctcatctac agtagactgc gtgtgcggag ccgcaagctc atcttttgcc
     2101 tctttcaatt caacgcaaag atcagaccag cttgctgcca tataatcact ctcagttaag
     2161 ttctctgcac ttttaattgc agtttcaagt gccgtcgtat ccaccgtttc ctcttcatcc
     2221 ttaacaatat ttgcatcggt taccttaaat tttaccgtgt aatcattgta accaagtgca
     2281 tataccgtga ttgtccagta tccggttccg tctgttcccg ccggcagttt acaacgcagt
     2341 gaatctgtaa gaccaagctg gattccctgt gctttgtgca tccagttatc tgatgcaaac
     2401 ttggttccat aagattgaag tggatcggta taagtactgt cggatccata ataagtccac
     2461 tctgctgcct gcatatcagc accaagtgca ccgtagccct ccccagtcag atcgacacga
     2521 agaaattctc cgtaagaacc gtttgcctct tttaccgttg ttgtaatatt ctctgctgtt
     2581 tttaatgcag cgtccttgat tccggaatcc gttccattgt ttactctcgc agcaaacgaa
     2641 aaacttccgt cctcattttg ggtaactgtt tttagaccat tggtgttttc tgttacttct
     2701 gcaaccagat ccgtatagtt tttcagattt ccctccgaaa atccaccgga taaagtactg
     2761 ccattttcca ctacggtata tgctgcttta aaagcttcat aatcttcagt ttttactttg
     2821 actggaacat atttaagtcc tgttaccaca tagctttcaa tctcattttt atcataggaa
     2881 aaagtctctc cgttcgtcag cacaccggtg gctgctcctg tataatatgc cactttgagc
     2941 tttgtaccat ctttttttgt aatttctgac gtacactgat aacttcccct gtgcagtccg
     3001 tgattggtgg ttgctctcga tacggtatca aatgcaccct tgtcaagctc agcgctgcat
     3061 aagcatactg atactcctgc gtttctcccg tttcactctt cacctcggtt ccagtgatct
     3121 gctctgcttc cactgcactt tcttctgcgg caaaaaccgt catggacgat gtccctgttg
     3181 ctgccaccat ggaaaaagct aatactccgg tcagaatccg ggcagataca ttttttcttt
     3241 tcattttttt aatcctcctt cattttgctt tatctatcaa aagttagttt ttgctaactc
     3301 tcaatcaaaa aattcacact gtcagattcc gatccacagg tataagaaaa gcagaaatac
     3361 cgcccttatg gcagtaaacc acaattcatt ccctttcaat tttccagaga caccacagtg
     3421 gatatttcct ttcggacaga taccggtaca tttctgacac ataaaacagt cgcctctcgt
     3481 ctcaatcgcc cccagttccg gcagtccgat atccgaggga cattttttcg tgcatgctgc
     3541 acagccagag atacagttct ccctttctct cctgagcgca aaatatggca gtactggaag
     3601 aagtgaaaat atcgctccca tcgggcacaa aatacggcag aaaaaacgct cctgcacgca
     3661 catgccacac aggatgagaa tgagctggac gactcccgca aaatatcctt ccaatgcaaa
     3721 atttcctgca tgaagcacgg aaaagacatc ccacggactg ctgccgtaag ttccatgcca
     3781 agcgtgaccg gtttcttttt taatttttta cagacccaga cataagctgc acgcacccag
     3841 tccgacagac ttccaaatgc acaggcaaat ccacagaaaa aacggccgaa cactacggta
     3901 gatgcacaga gtatgataag tgacagcaca aatgaagtga tttcaatctg tgcacccgtt
     3961 cccatctgtc cggcaatata tttcacaccg gaaaatgcag ccgtgaacgc tgacggaaaa
     4021 aatacaaaaa acaataactg tattcccgca aggatccatg ctggaatctg ctttttatgc
     4081 ttcttccaga aaacagctcc atttgtcttt cgttcctttg atatacaatt tttttgaatt
     4141 atttcaacta tattttttaa ctttgtacct tccaattttt tcctcactct tactgttctg
     4201 cctgtttcag tgcctcgctg acagcatccc tgatcccggt agaactgaac gttgcaccgc
     4261 ttacggtatc cacatctgcg gactgcgcct ctatgatctt tggaatgata tcttccgcca
     4321 tggataaata ggctttatcc tcgccgtctg cggaagttat ctcaatttcg ctgattgttc
     4381 cattttccac ggtcacaagc acggtgatca ttccgccaaa accttttgcc ctgccttccc
     4441 atctgccgtc cggataagtt ccttttatat cgacagtttt ttctttttcc tgctgcagtt
     4501 ttgtcacctg cgcctctaac tttgtgatct cttcatcctt gcttctgtcc tttaatacct
     4561 gattgtaacc tgccagtatc aatatgactg cagccagaca aaatgaccgg atcaaaaaat
     4621 ctttcatctg ctattctccc ttaatttttt gcctgctcca atgccttttg acaggcttca
     4681 atgattgctt ttgaggaaca ggttgcagac gataccgtat cgatcccttc tgtactgttc
     4741 ttttgtaata tctgactgac aacacctttt gacgcggctt ttatatagaa tgaattgctc
     4801 tttcctccgt ctccggaaat atctttgatc tccttgatct gatcgttttc gatcacaagc
     4861 gtgagcgtca gtttatattc atcaaaatct tcatcctcat ccggaatgca cacaacactg
     4921 accttataat caccattttt atagatcagc gttgtctcgc cactattctc ggtattttcc
     4981 gtatcatttt tttcagtatc tccgccactc tcagtatttt cacctctatt ttccgtatcc
     5041 tcgctgttat tttctgtatt ttcgctgttg ttttccgtat cctcactgtc tttttccgtg
     5101 tcttcgctgt tctttgtatc gtcatcaccg ctctggctgc cagagaacgt gtagtttcca
     5161 tcttttgccg cagcttttac caccacatcc acggcatgca gtactgcctt tgatgtacag
     5221 gttgcaccgg agaccgtatc caccccgtca attccgtttt tatttacgat ctgtgttaca
     5281 acccccggat gcttactgct gccggaaacc gcctttgtaa tataagtatc attctgggaa
     5341 tctccatccc cgtacacatc ggaaactgca atgatttttc cattctgaaa catcactttt
     5401 acatgcaggt tgtacgccgt aaaagcaccg ctctcatccg ggttacagac aacgactcca
     5461 taataggagc tcgcttttga tgtgtccggc ttttcggtat catcttctgt ttcggtatcc
     5521 gtctccccct ttttcgcttt tttggcagcc tccatcgcat ttgatactgc ctcaatgatt
     5581 cccctcgaac tatatgttgc accagaaact gtatctacat ccgtgctctg cttttctaaa
     5641 atacttgccg tcacgccctc ctttgtacgg ttaaaaaatc ctgcatcatc cgatgttttt
     5701 acaatataga tcccaacgat cgacttatct ttgatctcaa taaacaattt gaccggtccg
     5761 gcaaatccat ttccagttcc ttcataatag ccatcattca acgcaaaatc tccctttgcc
     5821 acaaccgttt gttcttcccc gtcactggaa gataacgcat cttttactgc atttatgatt
     5881 cctcttgaac tgtatgttgc accggaaacc gtatccacat ccgtactctg ctttgcaatg
     5941 atgctcgctg tcacaccttc ttttgcacga ttgaaaaagg cttcatcatc tgaattactc
     6001 aaaatgttga tcgaccggat cgtctgattt tttatcacaa ctgaaacttt gatcttaccg
     6061 gaaaatccgt tcgcactgcc ttcataagtt ccgtctgcaa gatcaaacgc accgtctaaa
     6121 acaacagccg cttccgtatt ttcaacttcc gtctcttcta ccgactccat accggcactt
     6181 tcgataatag tttccgtctc tgtcagttca tcttctgact ctgaaccata aaccggtgct
     6241 gtataatcac tcagggaaac cactgcacca attcccacca ccgctgccag aatgacgggt
     6301 acaattcctg aaatattttt catacttgtc tccataccaa atcattcgtt tatacttttt
     6361 tttcaatgat cttcgtatcc atttctgact caaaactgtc tgcgattccc tctgtgacat
     6421 agatttccct gtcatccgtc atcagaatca tgtcaaattc atcactgtga tttctccaat
     6481 attccgttgc ggcttctttc cccataataa aaacggatgt agaaagtccg tccgcaagcg
     6541 ttccatctgc tgtgacaatg gaaacggaaa ttaacccgtt ctccaccgga taaccggttt
     6601 tgggatctat gatatgatga tatgtcctgc cgtcctgctc aaaataacgt tcataaccgc
     6661 cggaagtgat caccgcctta tcctgtatgg aaagcacccc aagatactga tctgtatcct
     6721 gtgggtcctg cactgccacg cgccagctgc ttccatcctt ctttgttcca agtgcctgca
     6781 catttccacc aagattgacc agtccgctct tgatcccgca ggcacgaaaa atatccatga
     6841 tacgtgcaga agtataacct ttcgcgattc ctccaaagtc gatctgcatt ccgtcctttt
     6901 taaaagcgat ctgcgatgtc tctttatcat aagaaatatc cgatgcatct gtcagcgtga
     6961 gcagatcctt aagttcgtct gcgtccggca ccctgaaatt gtcatctgta aatccccatg
     7021 ctttcataac cggataaatc gcaatatcaa atgcaccatc tgtagcttca tacagatcca
     7081 gtgagcgttc caccagatat gctgtctcct ccgagagtat tccatctccg ctctgattga
     7141 ttttatatat ctcactgtcc tcatttcccg tagacaacaa ttgatccagc cggctgatct
     7201 catctgccgc ctgatccacg gcctctgtgg ctcctttgcc ataagcagtt accgtcatat
     7261 atgtgtccat tgcaaagaga tctctgctcg cctcggtatc tcccccatcg gtaccacagc
     7321 cacctgccac aaccatgact gctgcaaaaa tcagaaatat aatttttctt tttactttac
     7381 cttcgctcat tttccctcta actttggtta gtattttcta accattttag cttacaatgc
     7441 accagacgca aatgtcaatc tgcacttttg agactttttc tcattttgat ctccatttcc
     7501 catgttaccg caaaaaaatc ccctcacata cagacaacaa cctgcatata agaggattcc
     7561 tcaattatat tatatttcat tcaatttaat tatatttcaa tgcctttttg agagctgctg
     7621 aaatcgccgt tcccacaaca aagcagacaa ttgcctcaac tagtccgttg acacctacaa
     7681 atgctaaaat gaacagcaat gcattttttg caccaagagc tgatacaaat gactggatgt
     7741 attccgtatt gtaaaatcca atcactaaca ttcccataaa aaatgtggtg ttcagcagcg
     7801 gacaacaaag gttgctgatc gtaacagata cacctgacgg cagctttgtt ttacgaagtc
     7861 ctttatagat cagacctgtc agccatccca ttaaaatacg ggtaggtacg catactaaaa
     7921 atgttaaaaa tccgttgatt ccaagcagtg cagcaccaaa tgggctcatg ccgaagcact
     7981 ggatgaaact tgtaacaccg aacacgccgc ctaagattgc gcctgccgcc ggtcctaacg
     8041 taacagctcc taccgctacc ggaacgacga tcagcgtgat ctcaagacca gctgttctga
     8101 tatatccgat cggggtaaaa gccatgatga gaatgatagc aactaaaagt gccatctcca
     8161 caagatactt tgtggaaaac tttttctgag tcatagtctc ttcctctttt cttgtaatat
     8221 gtaattttac aacgttatta agtataacac ttgcatgctc attccgcaat cccgcatcca
     8281 tatttacttc gtaatgtcat gtttttattt tcatccaatt tttaccatat tccatccaat
     8341 taactatatt tcaatgcttt cttcaaagcc gcggaaattg cggttccgac gataaaacat
     8401 accaccgcct caaccagtcc gttgattccg acaaatgcta tcacaaacaa aaatggattg
     8461 ctgacaccaa gcgtctgaac aaacgactgg atgtactcgg tctggtaaaa cccaaatacc
     8521 agcatgctca taaaaaacgt ggtatttaaa agcggacagc acagattcgc gattgtgatt
     8581 gatacattcg atggtatttt tgttttgcgg attccctgat aaatcagtcc tgtcagccag
     8641 cccgtcagga tacgggtcgg tacatacagc aaaaatgcca gaaacggatt gatgctaaga
     8701 agcatcgcac caaatgcatt cagtccgaag cagcgcaaaa aactggcaag tccaaataca
     8761 cctcctaaaa ttgcaccagc cgcaggccct aaagtaacag ctcctaccgc caccggaaca
     8821 acgatcagtg taatctcaat acctgccgtc ttaatgtatc cgagtggggt aaaagccatg
     8881 ataagaatga tggcaactaa aagtgccatt tccacaagaa actttgtgga gaactttttc
     8941 ttgttcatag tcccttcctc ttttctcgtt tttatgtaat tttatacgaa acacaggacg
     9001 agccatttgt ggatcagtat aaatttccgg catcctgtat ctctgcaacg ttactaagta
     9061 taacatctgg cagaattttc cgcaatctta gattgcgtta atcctaaaat cctaaaattg
     9121 tgccattctc agaattgcac taccactttt ttatattttt aatgtttcct tatattatca
     9181 ataaatgcca ttgactttct gctttttgcg tgcaaagcta accatatatt tataagaaaa
     9241 gagtttttac catgatacag aaaaaaatgt acttaacacc gttccggtta tctgccttgg
     9301 tgccctgctt ttggggtcgg catacgggca gcttcacacg ccaatgacgc cgccaccgat
     9361 tacagctctc gcctcagata ttctgccgac gacaggcaat gtccgagcac ttgttgttgt
     9421 ggtcgatttt gcggatgcga aatatgatac agacagacgc ctgtccgatg aagaaatttc
     9481 ctcctatctg tttggcacag acaatactga attttatccc tgtgagagcc tgactagtta
     9541 ttttgaccgt gcttcatacg gatcactgca tatgaccgga aatgtatttc attacacagc
     9601 gaaaggtacg atcgcatcct acgaaaaaac ggcagatgga tcttaagact ggtcaataac
     9661 ggaaatggct cttaccggga cggtgacgtg atcggaggca ctgccacagg ctttggctgg
     9721 tatgacaaca acggacagtt gaccattgat accggatata cgatcacggt cgactccgta
     9781 tctgaggatc agaaaaactg taccataacg attttgacaa acaactaagt atattttccc
     9841 tcaactcctg accaagagcc tcaaatttcc tccaactcca aacaagaagc ctcaaatttt
     9901 ctccagctcc cagcaaaaag cccgtccttc ctgtcagctt cctcacagaa gccgcacacg
     9961 aaagacgggc tttctatctt acaggttatt cacctatgta ttatttgcct aactcagctt
    10021 ttaatgctgt tgttaaagca ggaacgatct tattcagatc accaaccaga ccgtaatcag
    10081 ctacgtcgaa gattggagca tcttcatctt tgttgattgc gatgatcagg tcagaatctt
    10141 ccataccagc tacgtgctgg attgctccgg aaataccgat tgcaaagtag atctgaggac
    10201 gaacagtttt accagtctga cctacctgta aatcaactgg taaccagcca ttctctacaa
    10261 ctgcacggga gcagcttaca gtaccgccaa gtacctctgc aagatcttcc aaaagtttga
    10321 agttctcttt ggaaccaact ccacgaccac cggaaacaag gatctttgct tccataatgt
    10381 ttgctgtgct cttaacagct tttacgatgt ttaagatctc aacatatctg ttgtttggtg
    10441 taaatcccgg gttgtactct acaacgtttg cttttgcacc gctgatcgga gcgatcttct
    10501 gcataacacc tggacgtaca gttgccatct gaggacggtt gtccgggcag gcaattgttg
    10561 cgatggtgtt accaccaaat gcaggacgtg tcattaataa ctggttgtgt ttctgctctg
    10621 attctttacc aggtgcagca accagtggga aatcaccgat ctcaagagat gtacagtctg
    10681 ctgtcagacc tgtttttact cttgcagaaa cagtaggtcc taagtcacgg ccgatagcag
    10741 ttgcaccaac taacatgatt tcaggtttga actcgttaat tacagatgct aatgcatgag
    10801 catacggctc tgttctgtag tcttttaatt caggatcatc tactacgata actttgtcag
    10861 caccatactc agctaactga tctgctaatc cttttacacc agaaccaatt aatacagcag
    10921 ttacgtctgt acctaaatct tttgctaagt ctttgccttt tccaagtaac tcaaatgcaa
    10981 cgccatctaa tacgttatct acctgctgtg caaagacaaa tactccttta tattcttcta
    11041 atgccatgtt attcaccact tttccttttt ttattagata atgtgtttct ctttgaattt
    11101 accgatcaaa tatgctacgg attctgccgg atcaaggttt actttctcac cagcaccctt
    11161 tgcaacttta tcagatgctt ttgcaatctt agtaggagat ccctttaaac caagatcaga
    11221 atcgtcaaca tcctttaagt ctgctctgcc ccaaacggtt acttctttgt caaatgcatc
    11281 gaagattccg cccggtgtca tgtaacgcgg ctcatttaat tcagaaagag cagttaccag
    11341 acaaggcatt tttgctttta actcatgata tctgtcttcg tactgacgtt ttacaacaac
    11401 agaatcaccc tctactttga tgtctgctgc gtaagagata accggaagtc caagatgctc
    11461 agcgatctga ggaccaacct gtgcagtatc accatcgata gcctgacgtc ctgtgatgat
    11521 caggtcatag tctaagttac gaagtgcacc agcgattgtg gtagatgttg cccatgtatc
    11581 agctccacct aatacgcggt ctgttacaag aactgcttca tcagcaccca ttgctaatgc
    11641 ttcacgaaga actgcatcag cctttggaag tcccatggta agaactgtaa cttttgctcc
    11701 tgtctcatct ttgattctaa gtgctgcttc aagaccagct ttatcatctg ggttcatgat
    11761 agcaagcatt gctgctctgt caagcgttcc atccgggttg aacttaacgc cgccctttgt
    11821 atcaggaacc tgtttaatac atactacgat attcatgtat ttttcactcc ttatgtttta
    11881 tatgtttaaa ttttacgcgg attgattcga aattacttta acagtgcacc ggagattacc
    11941 atacgctgga cttcgcttgt accctcgtag atctcagtga tcttagcatc acgcatcata
    12001 cgctctacat cgtactctct gatatatccg tatccaccga ataactgaac aacctcagtt
    12061 gtaacagcca ttgcagtctc tgctgcgaat aatttcgctt ttgcagcttc tacagaatat
    12121 actttctgag tagcttttgc cattgcagct ttgtaaacta aaagctgtgc agcttcgatt
    12181 cttgcagcca tatcagcaag tttgaactgt gtattctgct gctgtgcgat tgaacgtcca
    12241 aactgttttc tctcttttgt ataagcgata gctctgtcta atgcgccctc agcgatacca
    12301 agtgcctgag cagcaatacc gatacgtcca ccatcaagtg tgtgcattgc gatcgggaaa
    12361 ccttttcctt ccggtcctaa cagattctct ttcgggattc tgcagtcttc gaagatcagc
    12421 tcataagtag atgatccacg gatacccatc ttcttctctt ttgttccgaa tgagaagcct
    12481 ggagctccct tatcaacgat gaatgcagag aagtttttct tctttctgcc tctcttatcc
    12541 tctgtgatgc ttgtgatagc gattacaata taaacatctg caactttacc attggtaatg
    12601 aagcattttg atccatttaa tacccactcg tcaccatcta aaacagcttt tgtctgtgca
    12661 ccctgtgcat cagtaccagc ccccggctca gttaatgcga atgcacctaa cttctcgcct
    12721 gttgcaagtg gttttacata tttctgtttc tgctcttctg taccatatgt catgatcgga
    12781 tcgatgcaga gagatgtatg tgcagataca acaacacctg tggttgcgca aactttagat
    12841 aactcttcta cgcacataac gtatgtaaga ggatcacaac cctgtccacc atattctttc
    12901 ggaaccggga ttcccatgaa tccgtatttc tgcattttct taaccgtttc tgccgggaaa
    12961 gcttctgtct catctgtttc ctgtgcaagc ggttttactt ctgtttcagc aaattccttg
    13021 aataaggaac gcgccatttc atgtttcttg tctaaagtga aatccatgat ctaattttcc
    13081 tccataaata tttattttac tgagcatcga ccggagtctt tgtacgatca gcattgtata
    13141 catagaatcc cttgccgctc tttgctccaa ggttaccacc acgaaccatc ttacggatca
    13201 gtgggcatgc acgatatttg ctgtcgcctg tctctttgta aagtacatcc atgatagcaa
    13261 ggcagatatc aagaccgatg aaatcaccta actctaacgg tcccattggg tggttagcac
    13321 caagtttcat agctgcatcg ataccagcga tatcggaaac accttccatt ttgatgaatg
    13381 cagcttcatt gatcattggg attaagatac ggtttacaac gaaaccagct gcctcgttaa
    13441 cctgtaccgg agtttttcca atctcttcag agattttctt aatagctgca acagtttcat
    13501 ccggagtatt cacaccagcg ataacctcga tcagtttcat acggtctgcc gggttgaaga
    13561 aatgcatacc aaccattgga cggtttaatc cgtttccgat ctctgtgata gaaagactgg
    13621 atgtgttgga agcgaagata cattccggtt ttgcgatctc gtctaactct ttgaaagtag
    13681 ttttctttac ttccatgttc tcgaaagctg cctctacgat cagatcacag tctttgcaaa
    13741 ggtcttcttt taaacctgga gtaatttttg caaggatgcc gtcaacagct tcctgtgtca
    13801 tttttccttt ttctactaat cttgcataac cttttttgat cttctctttt ccgccttcag
    13861 cccactcctg cttgatatcg caaagtgcta cttcatatcc ttctacctga gcaaatgctt
    13921 ttgcaatgcc ctgtcccata gttcctgcac caataacgcc aaccttcatt tcggttacct
    13981 cctgaaaata attatgttaa ctgtatgttt gaaaaatgca atccccggga agtcattccc
    14041 cgggaattac tataaaaaac tattatctct caacgattgt agagcatccc ataccaccgc
    14101 cgatgcaaag tgtagcaaga cctctcttag cgtctctctt ctgcatctca tgcagtaatg
    14161 taacaaggat acggcatccg gaagcaccta ccgggtgtcc taatgcgatt gcaccaccgt
    14221 ttacgtttac ttttgacata tcgaatttca gatctcttgc tactgcgata gactgagctg
    14281 cgaatgcttc gtttgcttcg attaagtcca tatcatcaat ggataaacct gttttagcta
    14341 atacttttct tgtagaagca acaggaccaa cacccatgat ctctggctca actccgccaa
    14401 gagcacctgc tacccatgta gccattggtg taacaccaag ctcttttgct ttttcttcac
    14461 tcataacaac gattgctgct gcaccatcgt tgataccgga agcgttacct gctgtaacaa
    14521 gtccgccctc tttaccggag cagcatttta attttgctaa agactcagca gttgtaccgg
    14581 cacgtggtcc ctcatctttc acgaaatcaa cgatctcttt tttcacttta acaggaactg
    14641 gaacgatctc atcatcaaat ttgccttcag cgattgcttt ttcacatttc tgctggctgt
    14701 ttgctgagaa ctcgtcaagt tcttctcttg taatgccata tttatcacat acgttctctg
    14761 cagtgatcat catatgatag tggttgaatg catctgttaa tgcatcattt accattgtat
    14821 cgatgatcgt tgcattgttc atacgatatc caaaacgagc ttttgtcata gcatatggtg
    14881 ccatagacat gttttccata ccacctgcaa caacgatatc agcttcacct gccatgatca
    14941 tggtagctgc atcattgaca cattttaaac cagaaccaca aacaacgttg attgtgattg
    15001 ccggaacttc gattggcagt cctgctttga ttgctgcctg acgtgcaacg ttctgtccct
    15061 gagctgcctg gattacacaa cccattttta cctcgtcaac catctccggt ttaacgtttg
    15121 ctcttcttaa agcctctttg ataacgattg cacctaagtc tgctgctgga gtattgctta
    15181 atgctccacc cattttacc
//
