LOCUS       c00146_gnlPro..         7497 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IIJOBCDP_146.
ACCESSION   c00146_gnlPro..
VERSION     c00146_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:47:11
            Original ID  :: gnl|Prokka|IIJOBCDP_146
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7497
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7497
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[284:3257]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      285..3257
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7497
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7497
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             285..2513
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_1"
                     /sec_met_domain="Gly_radical (E-value: 3.9e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2529..3257
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.4e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3285..3728)
                     /locus_tag="ctg146_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVNELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3715..3882)
                     /locus_tag="ctg146_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(3903..4406)
                     /locus_tag="ctg146_5"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             4600..4830
                     /locus_tag="ctg146_6"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             4918..7035
                     /locus_tag="ctg146_7"
                     /transl_table=11
                     /translation="MEHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSITYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(7124..7255)
                     /locus_tag="ctg146_8"
                     /transl_table=11
                     /translation="MVGNLKKITVFFKFPTVFLRKKKEKVRVGEGKFLTFVFFFRRK"
     CDS             complement(7273..7497)
                     /locus_tag="ctg146_9"
                     /transl_table=11
                     /translation="RAEAEEKAYAFYTGQIIEDARKKAKITQAELARRIGSDRSYISRV
                     ESGQTEPKVSTFYRIMNALGCRIEFSMSL"
ORIGIN
        1 cggttttcaa gaccgccgca atcgaccact ctgccacctc tccaaaactt ccttttcaga
       61 agtgcttttc gttaaaagcg gtgcaaaggt acgaatatat tttaaactta caagagtttt
      121 cacaaaaaaa aatcacgaac atcatttcca aagaatagca atatctaggt attttatgtt
      181 gaaaagcata aaatagagca tatttatgct gattatatga aaaaacgttg taactttgtg
      241 cgctctaaag aaaaggataa ctacaacctc taattttatc atttatggaa ttaaataaga
      301 tctttaaaga cggtctttgg agcacagaaa tcaacgtaag agatttcgtt agtcacaaca
      361 tcactccgta ttatggagat gcttctttcc tcgaaggacc taccgaacgc actaaagctg
      421 tatggaaccg ctgcctcgaa gcactggcag aagaaagagc caataacgga gtacgctcac
      481 tggataatgt caccgtctca accatcactt ctcataaagc cggatatatc gacaaagaaa
      541 acgaactgat cgtcggtctg caaacagacg aacttctgaa acgtgcaatc aaacctttcg
      601 gaggtatcaa cgtggtgagc aaagcctgcc acgaaaacgg cgtggaagtg gacgaccgtg
      661 taaaagatat attcactcac taccgcaaga cgcacaacga cggagtattt gacgtatata
      721 ccgaagaaat ccgttcgttc cgttctctgg gattcctcac cggacttcct gacaactatg
      781 cccgcggacg tatcatcggc gactaccgcc gtatggctct ctatggtatc gaccgactga
      841 ttgaggcaaa aaaagaagac ttgcgtaacc ttaccggtcc gatgaccgaa gcccgcatcc
      901 gcctgcgcga agaagtggca gagcaaatca aggcattgaa agacatgaaa gtaatgggcg
      961 aatactacgg actcgacttg agccgtcctg cctacaccgc acaagaagca gtacaatggg
     1021 tatacatggc ttaccttgct gccgtaaaag aacaagacgg tgctgctatg tcactaggta
     1081 acgtctcttc gttcctcgac atttatatgg aatatgaatt gagcaaagga accatcacag
     1141 aatctttcgc acaggagctg atcgaccagt ttgtcatcaa actgcgtatg gtgcgtcacc
     1201 tgcgtatgca atcttacaac gacatcttcg ccggcgaccc gacatgggta accgaatccc
     1261 tgggtggacg cctcaacgac ggacgtacca aggtgacaaa gacttctttc cgctttctgc
     1321 aaacactcta caacctcggc ccttcaccgg aaccgaacct gactgtattg tggagcccgg
     1381 aactgccgga aggattcaaa gaattctgcg caaaagtatc tatcgacact tcttccatcc
     1441 aatatgaaaa cgacgacctg atgcgtgaag tgcgccagtc tgacgattac ggaatcgcat
     1501 gctgcgtatc ttatcaggaa atcggaaaac aaatccaatt cttcggcgca cgttgcaacc
     1561 tggcaaaagc cctgttgctt gccatcaacg gcggacgttg cgaaaacacc ggtacggtaa
     1621 tggtgaaaaa tatccccgta ctaaccagcg acacactgaa cttcgaagaa gtaatgagca
     1681 actacaagaa ggtattgaca gaaatcgccc gtgtttacaa cgaagcgatg aacatcatcc
     1741 attatatgca tgataagtat tactacgaaa aagcccaaat ggctcttgta gataccaacc
     1801 cacgcatcaa ccttgcttat ggtgtagccg gactttctat cgccctcgat tcactgtcgg
     1861 ccatcaaata tgccaaagtg accgcacgcc gcaacgacat cggtctgaca gaaggtttcg
     1921 atattcaagg cgaattccct tgcttcggta atgacaacga caaggtagac cacctgggtg
     1981 ttgacctggt ctatttcttc agcgaagaat tgaagaaact tccggtttac aaaaatgccc
     2041 gtcctacttt atctttgctc accatcactt ccaacgtgat gtatggcaag aagacaggtg
     2101 ctactcccga cggacgcgcc aaaggtgtcg cttttgctcc gggagccaac cctatgcacg
     2161 gacgtgacaa gaacggtgcc atcgcctctt tgagttccgt agcgaaatta cgttaccgcg
     2221 actcacagga cggtatcagc aacactttct ccatcgtccc caaatcactg ggagctactg
     2281 aagaggatcg cgtagaaaat ctggtgacaa tgatggatgg ctatttcacc aaaggcgctc
     2341 accacctgaa cgtgaacgta ctgaaccgtg aaatgcttta cgatgccatg gaacatccgg
     2401 aaaaatatcc gcaactcacc atccgtgttt ccggttatgc cgtaaacttc gtgaagttga
     2461 gccgcgaaca tcaattggaa gttatcagcc gtagcttcca cgaacgtatg taatcagatc
     2521 tactcaatat gacgataaac gtacattcat acgaaagtat gggaacattc gacgggccgg
     2581 gcttacggct cgtcgttttt cttcaaggat gcaatttccg ctgcctgtat tgcgccaacc
     2641 ctgacacaat agccgggaaa ggaggcacac ctactccacc ggaagaaatc gtccgcatgg
     2701 ctatgagcca acgtcctttt ttcgggaaac gcggaggagt cactttctcc ggaggagaac
     2761 ctacgtttca ggccaaagca ctggttcccc tggtgcgtga actgaaagaa aagggaatac
     2821 atgtatgcat agacagcaac ggcggtatct ggaacgaaga agtagaagaa cttttcaaac
     2881 tgacggatct tgtattgctg gatattaaag agttcaatcc ggcacgccat caagcactga
     2941 ccggaagaag taacgagcaa accatccgca cggcagcatg gctggaagaa aacgagaaac
     3001 ctttctggct gcgttatgta ttagtgcccg gatacagcga tttcgaagag gatatccgcc
     3061 gcttgggaga ggctctggga aaatataaga tggtgcaacg agtggaaatc ctgccttatc
     3121 atacattagg cgtacataag tatgaagcga tggagcagga atacaagctg aaagacgtaa
     3181 aagaaaatac ccccgaacaa cttgaaaaag ccgcggaggt atttaaagag tatttcacta
     3241 ctgtggtggt caattaacca ccacagtaat aaataggata aaaatcacca tgtctctgct
     3301 ttcacctcga aacagggaca ctctttgatc cgctcccaag ggtcaacaat tccattataa
     3361 ttggtgtcag gactcaagtc ccgatgaccg accacctttg catccggata acgctgcaac
     3421 aactcgttta ccagacgatg catcgcctct ttctggcggg aagtacgcgt atctgctgcc
     3481 tttccgttct catccagccc gccttcatag cacactccta aactaatcgc attaaaaccg
     3541 cgtgcatgag cacccaccgt atcttcatcc ctcatcggct ctatctgtcc cgacttgcgg
     3601 atataataat gataccccca acaagaaaag ccccgaaagc gatgggcagt gtccacatcc
     3661 ttcgccgtga agtcgcgatc agcacgggta gcagagcagt gaatcacaat cagactaatc
     3721 tttctcatcg tccttctccc tttccgtcgt tttcacatta cacacttcct tatgggtagc
     3781 attgaagcgc acctctctca caattctgtt ccgctttctt tttccgaaga aaggcacaag
     3841 gaacataatt acatccaaaa tcttagttac aatttctttc atttgattca atattaaagt
     3901 tatcatgctg ccggatccgg agcttctccg cttccaccac cgtctgaacc accaccggaa
     3961 cctttgtcat cgtcttctcc ttcattgccg ctaccgcccg tttgggccac cgcacgcgtc
     4021 gttacctgct ggtagctaag aatcttcgtg atgtccgcca acgtctttcc gggacgaaaa
     4081 ataatatggg cagccgtaat cagcgaactt gaaaactcct tgaccgacac agctccttca
     4141 ctgttcaacc cgatctgcaa actacccaaa tcacccagcc ggactattct tcccgctttc
     4201 aacgcacgct tcgcctcata aagaataccc gaaatggcgg cacgcacatc tgtctccgta
     4261 caggtagacc ggctggtgat tccctcgcac aactcttcaa aatccaactc tcccgaagcc
     4321 tgtgcctgcg cataatactt tgccgactca tcctttttgg caggattctt gcgcggcact
     4381 actgaataag ttactgtcat tgccataata aaataatttt aaattttcat acatttcgaa
     4441 cgcgttcctt atttgttaac gacataaaga taacacattt tccatcctcc ggattcccca
     4501 acgaagtttc ctgatattct atgaataata atgacgttat tcttttaata agccattaat
     4561 tttcagtaac tttatagcat aaaccaaata ccattcatca tgtattatcc ggatcagccg
     4621 tttcatatcc gcacttataa aaagtcggaa ctagctcatc tttacaaccc caacgtatgc
     4681 cttaaagtag ccttacaaat actacgtcgt tggattgtct acaaccttcc gctcctgcaa
     4741 gaactcgaac aagaaggcta tcgtgcccgc aaccgccttc tctctccccg gcaagtggca
     4801 acgatcatac gctatttggg agaaccctag gaacttaatc aaaaaaacga aaagttccat
     4861 tctattattt tacaaacaaa tttctttgca accgcaaatt aaaaagaaca aaccaacatg
     4921 gagcatttta ccatctttca agacttttac cgcgtcatag ccgaaatgac agaagaagaa
     4981 atcgtatcgg ccatcagcaa cggcacatac cgggaaacag tggaggacgt gcgccgaatc
     5041 ttcgcggaac aaggcgagaa agcagcaaac gaaaagaaaa tagaactacc ggaaatcacc
     5101 ttttcagcca actaccgggg aggacgttca aacgcaactc tcgtcaagta tctgggatac
     5161 atcgtggtag acatcgacca tcagacacag gaagcattgg cacgcatcct cgcactggca
     5221 aaaaagtgcg cccacacccg catcgctttc atcagcccca aaggaatggg actgaaaatc
     5281 gtggtacgtg tatgccggac agacgggaca ttgcccgaaa caattcagga aattgaagat
     5341 ttccatcatg ccgcttatca caaaatcgct tccttttatg cccagctctg cggagttgag
     5401 gtcgacactt ccggacagga tgttagccgc acttgcctgt tctcttacga tccggatatt
     5461 tatttcaatc ccgatgccac agctgtcatc gtggaacagc caccgatgtt cttcaagtct
     5521 caaaagaaga aaagggcatc cggcaaaaag aaaaagacga cagcaccaga caacaatccc
     5581 ctcaccgaac aagtggcact caactaccat tcctcgcacg cttccttaat ggtgactctg
     5641 aactattatc acaataaatc cgaaaagtac gtcaccggca accggaacaa ctacctgcat
     5701 tgcctggctt gtatgtacaa tcgctacggc gtcccccagg aagaggcggc agcttttatc
     5761 aagagccagt tcaccgatct tcccgcggat gaaatggacg cactgatcgg gagtgcttac
     5821 ggacacaacg aggaattcga cacccgcaag ctgaatagta cgcagaagcg aatattacag
     5881 attgagcaac atatcaaaga aaattacgat acacgctaca acgaagtgct tcatatcatg
     5941 gaatatcgca gaaggaaaac ggacacggag caaccggaac ctttccatat tctcgacgaa
     6001 atgatggaaa acagcatttg gatggagatg aacgaactgg gctattcgtg caccgtcaag
     6061 acgattcaga atctgatcta ctccgacttc agcatcacct accatcctat tcgcgagtat
     6121 ttagactcgc tgcctgaatg ggacggaacg gattatatcg gtatccttgc caacagcgtg
     6181 catactagtc accagaaatt ttgggtagaa tgtctggaac gttatctggt gggaatgtgc
     6241 gctgccgcca cacaagacga tgtggtgaac cacactgttt tgttgctttg cagcgaaata
     6301 cagaacatcg gaaagactac gtttatcaac aacctgctgc cgcccgaatt gcgcgcctat
     6361 ctttctaccg gtcttatcaa cccgaacaac aaggatgatc tggccaaaat agcgcaggca
     6421 atgcttatca atctggatga gtttgaaggg atgaacggac gggaactgaa tatcttcaag
     6481 gatttggtta cccgcaaagt aatcagcatc cgcctgccat acgcgcggcg ttcgcagaac
     6541 tttcctcaca cggcttcttt tgccggcaca tgcaattatc aggaagtgct tcacgacacc
     6601 actggtaacc gccgttttct ctgctttcat gtcaactcga tggagtttat caaaataaac
     6661 tacgcgcagt tgtatgcaca aatcaagtat ctactcaaca agccggggta tcagtattgg
     6721 ttcacccaat cggaaaactc acggattgaa gagaacaacg aggatttcat tttccactca
     6781 ccggaagaag aactggtgtt gacacatatc cgcaaaccgg aacgtttcga aaaggtgcat
     6841 tatctgacgg tcactgaaat tgcggaattg attcgcgaac gaacggggta tcaatactct
     6901 cacggaacca aagcccaact aggaaaagtg atgtccaaac atggcttcga atttcataag
     6961 ggaaaaaacg ggagaagata cactgtattc attattgata cggaacaggt gaaatcaaac
     7021 aggctatatg aataagattt cgtctccttc gcacgcaaaa aggtgagggt tggtgagggt
     7081 acttttgcaa cttttcattt ttatatttct tatttctatt cttctatttt cttctaaaaa
     7141 aaaatacgaa tgttagaaat ttaccctcac ctaccctcac cttttccttt ttcttcctca
     7201 aaaaaacggt gggaaatttg aaaaagacag ttatcttttt caaatttccc accatgtttt
     7261 tttaatcgct tcttataaag acatcgaaaa ttcgatccta caacctaacg cattcatgat
     7321 gcgatagaaa gtagaaacct taggttctgt ctgaccactt tccactcttg aaatataaga
     7381 acggtcggaa ccgatacgcc gggctaactc tgcttgagta atcttagcct ttttccttgc
     7441 gtcttcgata atttgcccgg tgtagaaagc atacgctttt tcttctgctt ccgcacg
//
