LOCUS       c00249_gnlPro..         4897 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DBFMDGLB_249.
ACCESSION   c00249_gnlPro..
VERSION     c00249_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:03:01
            Original ID  :: gnl|Prokka|DBFMDGLB_249
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4897
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4897
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[80:3095]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      81..3095
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4897
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4897
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(81..821)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.1e-29, bitscore:
                     90.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             complement(840..3095)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_2"
                     /sec_met_domain="Gly_radical (E-value: 2.4e-42, bitscore:
                     131.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.9e-269, bitscore:
                     881.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNFEQWNGFKGNRWKEKIDVRNFIGMNYTPYDGDASFLEGPTEA
                     TNKLWGKLQALQKEERAKGGVLDMETEVVTSLTAYGPGYIDEETKDLEKVVGLQTDKPL
                     KRAFMPYGGIKMAEQACETYGYKVSDKIKDVFHNYEFKTHNQGVFDIYTPEMKVARHNK
                     ILTGLPDTYGRGRIVGDYRRVALYGIDALIEGKQKDFAACDRQGMRRYDFQLREEIADQ
                     IRALKGMKVMAESYGYDISKPAKDAREAFQWLYFGYLAAIKTQNGAAMSVGRISTFLDI
                     YIERDLQNGTLTEKEAQELVDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVAGMGQD
                     GRSMVTKNDYRFLHTLEDMGPAPEPNLTVLYSSRLPENFKKYAANISVTTSSVQYENDD
                     VMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYAINGGVDEKTKQQVGPNYQ
                     PITSEYLDYDEVIAKYDKMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTKVERSF
                     ATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDFPRYGNDDDRADEIATTLL
                     STFLEKLKHIHTYRDSKPTTSILTITSNVVYGKATGSLPDGRKAGEPLAPGANPSYGAE
                     QNGLLASLNSVAKLDYEDALDGISNTQTINPDALGHSDDERSENLVHVLDGYFDQGAHH
                     LNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     CDS             complement(3507..4895)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg249_3"
                     /sec_met_domain="HMGL-like (E-value: 1.2e-53, bitscore:
                     170.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QNLIPQDVTIQVLTQAREHIIRKTFEAVKGAPKAIVHVYNSTSVA
                     QREQVFKKSKEEILKIAVDGAALLKKLADETEGNFQFEYSPESFTGTEPEYALEVCNAV
                     LDVWQPTADNKCIINLPVTVQHSMPHVYASQVEYMCENLKYRENVIVSLHPHNDRGCGV
                     ADSEMGLLAGADRIEGTLFGNGERTGNVDIVTLGMNMYSQGVDPKLDFSDMPHICEIYE
                     ECTGMKVGERSPYSGALVFAAFSGSHQDAIAKGMHWRDDKDPDHWNIPYLPIDPTDVGR
                     NYDADVIRINSQSGKGGVGYILETKFGLNLPPKMREAMGYATKAVSDHKHKELHPDEIF
                     NLFKQTFENITEPYSINEVHFQQQDGGIVTKVTSTFRGKTITTEASGNGRLDAVSNALK
                     KAYELKYSLETYQEHALERSSSSKAIAYVGIKKPDGTLAWGAGVDVDIIRASIDALVTA
                     INNR"
ORIGIN
        1 ttcgcgtccg tcccctttct aaacaaaaaa caaagctgcc atacccccaa aaagaggata
       61 tgacagctat atatttttac tcagatagct ccaagaatct tccgggcatt atcaatcctt
      121 tcctgcgtcg gaggattaac cccctccagt ggataagaaa gccccagatt ctcccattta
      181 aacttaccaa gcgtatgata agggagcacc tccactctct ccacattgga aagagtatgg
      241 ataaaatcag ctaaccggtg cagatattca tccttatcac ttccgcccgg cacaagcaca
      301 tgacggatcc atacaggttt tttcatatca gaaagttccc gcgccatcgc aagaatattc
      361 ttattggaat gaccggtaag cttaatatgc tcctgctcgt caatttgctt gatatcaagc
      421 attacaagat ctgtatactt cataagctct agccatccgg aatgccatgg ttcctgatca
      481 gtgaagggat ttccactggt atcaagattc gtatgcacac cctctgcctt tgccttctta
      541 aaaaattctg taagaaaatc catctgaagc aagggctcac caccgcttac agtgataccg
      601 cctttgctgc cccagtagcc cttataacgt aaagctttct taagaagctc atctgccgta
      661 taaagctctc cctgcttcat cttccaggta tccggattat gacagaactg acatctcata
      721 gcacagccgc ttaaaaaaat cacatatctt actccgggtc cgtcaacgga cccgaagctt
      781 tctaaagaat gaacatagcc ttttatctgt ttattatcca ttatatcatc atccattaat
      841 tacattctgt cgtggcaggt tctggcaata acgtctaact gctgctctct tgtaaggtcg
      901 atgaacttaa cagcatatcc ggatacacgg attgtgaagt ttgcatactc ttctttttcc
      961 ggatgttcca tagcgtcgat cagtttctct ttaccaaata cgtttacatt gaggtgatga
     1021 gcaccctgat caaagtatcc gtcgagtaca tgtaccagat tctctgaacg ctcgtcatca
     1081 ctgtgtccca gagcatcagg attgattgtc tgagtattgg agataccgtc aagagcatct
     1141 tcatagtcaa gcttggcaac agagttcagg gaagcaagaa gaccattctg ctctgcaccg
     1201 taagatgggt ttgcgccagg tgcaagaggt tcccctgctt ttcttccatc cggaagagat
     1261 cctgtagcct taccataaac aacgttggaa gtaatagtaa ggatagatgt ggttggcttg
     1321 gaatcacggt aagtatggat gtgtttcagt ttctcaagga atgtagacag aagagttgtt
     1381 gcaatctcgt ctgctctgtc atcatcatta ccataacgtg ggaagtcacc ctcaacttgg
     1441 aaatctgttg taataccgtc ttcatcacgg atagctttaa cttttgcata tttgatagcg
     1501 gacagggagt ctactacatg agagaatcct gcgatacctg ttgcgaaaga acgctcaacc
     1561 tttgtatcga tcagagccat ctctgctgct tcgtagtagt atttgtcatg catgtagtga
     1621 atcatgttca gtgttccgac atacagatga gccagccagt ccatcatttt gtcatatttt
     1681 gcaattactt catcatagtc aagatattca gaagtgatcg gctgatagtt tggtcctacc
     1741 tgctgttttg tcttctcatc aacaccgccg ttgatcgcat aaagaaggca ttttgcaagg
     1801 tttgcacgag cgccgaagaa ctgcatctct ttacctgtct ctgtagcaga tacacagcaa
     1861 cagatgctgt agtcatcgcc ccataccgga cgcataacat catcattctc atactgaaca
     1921 gaacttgttg taacagaaat atttgcagca tatttcttga agttctcagg cagtcttgaa
     1981 gaataaagta ctgtaaggtt tggttccgga gccggtccca tatcttccag tgtgtgcagg
     2041 aaacgatagt cgttctttgt taccatggaa cgtccgtcct gtcccatacc tgctacttcc
     2101 agagttgccc ataccgggtc accggagaat aactgattgt aagaagggat acgtgcaaat
     2161 ttaaccatac ggaatttcat taccatgtgg tcaacaagct cctgagcttc tttctctgta
     2221 agagttccgt tctgaagatc tctttcaata taaatatcaa ggaatgtgga aatacgtccg
     2281 acactcattg cagcaccgtt ctgagttttg atggctgcaa gatatccgaa gtacagccac
     2341 tggaaagctt ctcttgcatc tttggccggt ttggagatat catatccata agattcagcc
     2401 ataactttca tgcctttcag agcacggatc tgatcagcga tctcttcacg aagctggaag
     2461 tcataacgtc tcataccctg acggtcgcat gctgcgaagt ctttctgttt tccttcgatc
     2521 agagcatcga ttccgtacag agccacacgt ctgtagtcgc caacgatacg tccacgtccg
     2581 taagtatccg gaagacctgt aaggatcttg ttgtgacgtg ctactttcat ctcaggtgtg
     2641 tagatatcaa atacaccctg gttgtgagtt ttaaattcat agttgtggaa aacatctttg
     2701 atcttgtcac ttactttata tccgtaagtt tcacaagcct gctctgccat tttgataccg
     2761 ccgtatggca tgaaagcacg tttcagaggt ttatctgtct gaagaccaac aactttttca
     2821 aggtcttttg tttcttcatc aatatatccg ggaccatatg ctgtcagaga tgtaactacc
     2881 tctgtctcca tatcaagaac tccgcctttg gcacgttctt ctttctgaag tgcctggagc
     2941 ttgccccaca gtttgtttgt agcttctgta ggtccttcca ggaaagaagc atcaccgtca
     3001 tatggtgtat agttcattcc aataaagtta cgtacatcga ttttttcttt ccaacggttg
     3061 cccttaaagc cattccactg ttcgaaattt ttcattcttt tacctctttc tctaaaatat
     3121 ttccgcgcat ctctcactgg tctggagtga gataataatt tgtaaattgt acttatttag
     3181 gacacttttc tgtccacgct gtcagtatac gtaaacctga gtgttatgtc aattaatttc
     3241 ccttatgatt ttaattaaat acaattctga tagcttttct ccggagttcg cttctttatt
     3301 gtactttatc gcgtacatta agactttttt gtatttttat aaatacaaaa agataccggt
     3361 caggaagtcc tcgggacaat ctgaccggta tctgattttg atattttatg taaactataa
     3421 atacagtacc ataaggatta cggatattag cctaacctgc agaaatcaaa aaaagcagga
     3481 aagaatcagg cgtgtcggaa cacactttag cggttattga ttgctgttac aagagcatca
     3541 atagatgcgc gaatgatatc tacatctacg cctgcacccc aggcaagggt tccatccggt
     3601 tttttaattc ccacataagc aatggctttg gaacttgagc tgcgctccag cgcatgctcc
     3661 tgatatgttt ccagggaata tttcagttca tacgccttct tcaatgcatt gctgactgca
     3721 tcaagacgtc cgttaccgga tgcctctgta gtgattgtct ttccgcggaa agtagaggta
     3781 acctttgtta caataccgcc atcttgctgc tggaaatgta cttcattgat gctgtatggt
     3841 tctgtaatat tttcaaaggt ctgtttaaac agattgaaaa tttcgtcagg atgaagctct
     3901 ttatgcttgt gatccgaaac agcttttgtg gcatatccca ttgcctcacg cattttcgga
     3961 ggaaggttaa gaccgaattt ggtctcaagg atatatccta cgccgccttt accggactgg
     4021 ctgttgatgc ggataacatc tgcgtcataa tttcttccca catctgtcgg atcgatcgga
     4081 agatacggaa tattccagtg atccggatcc ttgtcatctc tccagtgcat acccttggca
     4141 attgcatcct gatgagagcc ggaaaatgcg gcaaatacaa gtgcaccact gtacggactt
     4201 ctttcgccta ctttcattcc ggtacattct tcatatattt cacagatatg tggcatatca
     4261 gagaaatcaa gttttggatc tacaccctga gaatacatat tcataccaag tgtaacaata
     4321 tctacatttc cggtacgctc accgtttcca aacagagtac cctcgattct gtctgcacct
     4381 gcaagtaatc ccatttcaga atctgcaaca ccgcatcctc tgtcattgtg cggatggaga
     4441 gatacgatta cattctcgcg gtatttcaga ttttcacaca tatattctac ctggcttgca
     4501 tatacatgcg gcatggaatg ctgtacagtt acaggcagat tgatgatgca tttgttatct
     4561 gctgtaggct gccatacatc aagaactgca ttgcatactt ccagagcgta ttccggttct
     4621 gtgcctgtaa agctttcagg gctgtactca aactggaaat ttccttctgt ttcatcagcc
     4681 agtttcttta ataatgcggc accatccact gcaatcttta agatttcttc tttggatttc
     4741 ttaaatacct gttctctctg agctacagag gtggaattgt atacatgtac aattgccttc
     4801 ggtgcgcctt tgacagcttc aaaagttttg cggatgatat gctctctggc ctgagtcaga
     4861 acctgaatag ttacatcctg tgggatcaga ttctgct
//
