LOCUS       c00026_gnlPro..        15173 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MAGDFHNO_26.
ACCESSION   c00026_gnlPro..
VERSION     c00026_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 16:28:49
            Original ID  :: gnl|Prokka|MAGDFHNO_26
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 21434
            Orig. end    :: 36607
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15173
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[31434:36606]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..15172
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..15173
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15173
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             955..1305
                     /locus_tag="ctg26_25"
                     /transl_table=11
                     /translation="MTLSECYEEMGADLHEVLRRLKTEERIGKFLKLILTDTNYESLCK
                     ALEEKNYEQAFTHIHNLKGLAMNLGLTPLLVPAQELCEELRDGEPERDLKPLLDEVAAA
                     YQKVLGIVERLD"
     CDS             1450..2070
                     /locus_tag="ctg26_26"
                     /transl_table=11
                     /translation="VQVILLIIAVSVDVFVACVACGSEQIKIGSAAALCISTVCSGILG
                     ISLYAGMALQGVLLKKYALYLSFFGLLAVGIFKLTEYAIRKYIERHKFLCKKVKISFSQ
                     LSFILSIYNNPVMADRDHSATMSCVEAVFFALAMSLDGMFGGLGAGFTGKNIYFTVMGS
                     FVVSFCAVLCGCRIGMQASKRWKTDVSWLGGVLFVILAFQRII"
     CDS             2363..3487
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg26_27"
                     /sec_met_domain="GATase (E-value: 6.1e-48, bitscore: 154.3,
                     seeds: 97, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKEEKRRRFFFWKKSERTGKRMKAHLILEDGNVFTGTSIGSTRE
                     VISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGNYGITPDMESGRPWPDGYIVREL
                     SRMPSNFREEGTIQDFLTKYDIPGIAGIDTRALTKILREKGTMNGMITTNNNYNIEEII
                     PRLKAYTTGNVVDKVTCSEKRVLKGNGKRVALLDLGAKNNIAQSLNKRGCEVTIYPAHT
                     TAEEILATNPDGIMLSNGPGDPKECKEIIAEIKKLYDSDTPIFAICLGHQLMALATGAD
                     THKMKYGHRGGNHPVKDLETGRVYISSQNHGYVVDTDTLDPKIAKPAFVNVNDGTNEGL
                     SYVGKNIFTVQFHPEACPGPQDSAYLFDRFISMM"
     CDS             3534..6740
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CPSase_L_D2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg26_28"
                     /sec_met_domain="CPSase_L_D2 (E-value: 1.3e-83, bitscore:
                     270.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRNHDIKKVLVIGSGPIVIGQAAEFDYAGTQACRSLKEEGVEVV
                     LVNSNPATIMTDKDIADQVYIEPLTVSVLEKIIAKEKPDSVLPTLGGQAGLNLGMELEE
                     KGILEKYNVRLIGTTAETIFKAEDRQAFKDTMEKIGEPCAASQVVHNVQDGIKFTNTIG
                     YPVVLRPAYTLGGSGGGIAHNETELVEILTNGLRLSRVGEVLVERCIAGWKEIEYEVMR
                     DANGNCITVCNMENIDPVGVHTGDSIVVAPSQTLGDKEYQMLRTSALNIISELNITGGC
                     NVQYALNPDSFEYCVIEVNPRVSRSSALASKATGYPIAKVAAKIALGYTLDEIKNAITG
                     KTYASFEPMLDYCVVKIPRLPFDKFITAKRTLTTQMKATGEVMSICNNFEGALMKAIRS
                     LEQHVDSLMSYDFTGLSDKELDEQLHVVDDRRIWVIAEALRRGVSYDKIHEITKIDRWF
                     IDKLAILVEMENQLKNEPLTVELLKEAKRIEFPDSVIAKLTGKTEKEIHDMRYENGIVA
                     AFKMVDTCAAEFEASTPYYYSVFGSENEAIETNPKKKVLVLGSGPIRIGQGIEFDYCSV
                     HCTWAFAKEGWETVIINNNPETVSTDFDIADKLYFEPLTPEDVESIVKLEKPDGAVVQF
                     GGQTAIKLTESLMKMGVKILGTKAEDVDAAEDRELFDEILEKTQIPRAAGGTVFTAEEA
                     KEVANRLGYPVLVRPSYVLGGQGMKIAFNDDEIEEFIGIINRIAQDHPILVDKYLQGKE
                     IEVDAVCDGTDILIPGIMEHIERTGVHSGDSISVYPAPTISDHVKETIVEYTKRLAQAL
                     HVIGLINIQFIAMDEQVYVIEVNPRSSRTVPYISKVTGIPIVDLATKVIIGDTIRGLGY
                     EPGLAPTADYIAIKMPVFSFEKLRGAEISLGPEMKSTGECLGIAKTFNEALYKAFLGAG
                     VTLPKYKQMIMTVKDADKPEAVGVAKRFEALGYKIYATRSTAKYLQEHGVNALRVNKIS
                     QESPNVMDLILGHKIDLVIDTPTQGNGDKTRDGFLIRRNAIETGVYCITAMDTANALAR
                     SLETATDDLTPVDIATVKNL"
     CDS             complement(6995..8290)
                     /locus_tag="ctg26_29"
                     /transl_table=11
                     /translation="MQFVKTADLKPGMRLAKPIYNKMGVLLYERDTLLTMQGINSIENF
                     GLIGIFILEPAEPVPPLSREDLEFEQFQTIYVFKLKENMDRLQANQAPVSLSDLVRDIQ
                     THYGSLDHKLNFAQNLRSSADFVYKHSISVGILCAMITNQMHLPAAEQTALITAALLYD
                     FGYLYVPQTVLDKGDDLTTADKDFIQLNLERGYETLRPDRGDYKFPVHTLDIIQQIIFK
                     NSRTLKVKEPADNIRLLVDILAVADEFDHLTAMNINKPPISEIAAMGRLKKDKATYNPL
                     VLSALAECIHILPTGACVDLSDGEKALVLAENPADFLHPMVLKFSTNMIYDLSDPVINK
                     SLKITDIMKTMDNRIALDKDSVKHFVSDAYIKETADRFRKVKLQIAQHNQSKLEQKAAS
                     ALMSSASVIPQDALGTEKPLTQKKPRKKMKLR"
     CDS             8501..9655
                     /locus_tag="ctg26_30"
                     /transl_table=11
                     /translation="MRHNFLRRILGGILTGCILVTATVHAGTTQKDIDKAKDQITDLKK
                     QKEDAKDQVDSLTNEKQGLESDLSGLNGQLNTITSDMNQLEQQAAQKEDEINTAAEDLK
                     RSEERSAEQYEDMKVRIQYIYENGNQSVWDMLIHVSSIAEFLNQAEYTSAINQYDRNML
                     TAYQELTEQIKKQKKELEEQKEELTVLQQEKQKEQEKVNSLIEDTQKKIQESSEKIEDA
                     QSDVESIDAKIEKMIEYEKQLEIQKAKEDAARLAAIKKQEQENRSNVTYAPAQSDAYLL
                     AAIIQCEAWGEPYDGKLAVGSVIMNRVRSSYFPNTVSGVIYQGGQFSPVASGRLAYRLE
                     QGVASDCMSAAQAVLGGTSTTNCLYFRVNNGIIEGTVIGNHVFY"
     CDS             10001..11182
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg26_31"
                     /sec_met_domain="Thiolase_N (E-value: 1.6e-97, bitscore:
                     317.1, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 5e-51, bitscore:
                     162.8, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKKVVLAGACRTAIGKMGGALSNTPAADLGAIVIKEALRRANVK
                     PEMVDEVKMGCVIQAAQGQNVARQAAIKAGLPIEVPAITINVVCGSGLKCVNDAATMIM
                     AGEADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITAE
                     NVCDKYGITREELDEFSANSQQKCEKAIAEGKFDDEIVPVPVKVKKEIVDFVKDEGPRA
                     GTTAESLAKLKCCSGKEGGLVTAGNASGINDGAAAIVVMSEEKAKELGVTPMATWVAGA
                     LGGVEPEIMGVGPVASTRKVLAKTGLSIDDMDLIEANEAFAAQSIAVARDLKFDMSKVN
                     VNGGAIALGHPVGASGCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVER"
     CDS             11276..12148
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg26_32"
                     /sec_met_domain="HCDH (E-value: 5.9e-35, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 4.3e-63, bitscore: 203.6,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYEVALCDIKQEWAEGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFENMEVKKTTFKELDE
                     IAKPDCIFASNTSSLSITEIGNGLNRPMVGMHFFNPADRMKLIEVIAGVNTPDETVSAI
                     KKISEEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             12178..13332
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg26_33"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.9e-52, bitscore:
                     168.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 7.1e-31, bitscore:
                     97.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.4e-39, bitscore:
                     126.6, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFKEFAETEVKPLAQETDETEAFPAETVKKM
                     QKYGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVVVSAHTSLCIDPIMTYGTE
                     EQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSITEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGPEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRSIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             13389..14171
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 159.1;
                     E-value: 3.7e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg26_34"
                     /sec_met_domain="ETF (E-value: 2.1e-49, bitscore: 159.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLAIMNPDDKAGLEAA
                     LRIKDETGAKVTVLTMGLPKADAVLREALAMGADEAVLVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAADIKVEGDSVVVKRQYEDRY
                     HELKAKMPCLVTALSELNEPRYMTPGGIFDAFDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVAKGAGEKVNLDPAESVAYLIGKFKEKHII"
     CDS             14189..15172
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 161.5;
                     E-value: 6.5e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg26_35"
                     /sec_met_domain="ETF (E-value: 3.7e-50, bitscore: 161.5,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 1.7e-38, bitscore:
                     121.8, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VNNMALEEYKGVFVFAQQVDNVLDGVAFELLGKGKDLAKDLGTDV
                     TAVLIGSGVKGLADQLAEYGADKVIVVDDPELKDYRTEPYAHALASVINEFKPEIMLVG
                     ATAIGRDLGPTVSARVKTGLTADCTSLEIGDFPLVAAPGKESEQKHNQLLMTRPAFGGN
                     TIATIACPDNRPQMATVRPGVMQKIAPISGAKANVVEYNPGFTPNNRYVEILNIVKAVK
                     STANIMEAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQT
                     GKTVRPQIYFAIGISGAIQHVAGMEDSDLIIAINKDEDAPIFDVADY"
ORIGIN
        1 ttaaattcct gtctgactgc tgatgagatt gaggaaatca caggaatcgt caatggaaca
       61 acgaactata tgatgactga aatgtcagaa aaaggtgctg attttgatga ggtattaaaa
      121 gatgcacagg caaaaggata tgcggagaaa gatccgacag ccgatattga agggtatgat
      181 gcctgcagaa agattgcaat tctgacatca cttgtctgtg gacagcaggt agattttgag
      241 gatatccaca cagagggtat cacacatatt tctgcaaccg atatcaaata tgcgaaagcc
      301 atgggaagag caatcaaact tcttgctacg agcaaaaaaa cagatggcgg atatgtggca
      361 atggtagcac cgttcttact gccgaaagag catccgttat acaatgtaaa cggcgtattc
      421 aatgcaattt ttgttcatgg aaatgtactt ggggatgcaa tgttttacgg aagcggtgcc
      481 ggaaaacttc cgacagccag tgcggttgta gcagatgttg tggagatggc aaagaatgtt
      541 gacaagaata taccggttga gtggagctca aagaaattag agctggtaga ttaccgcagc
      601 gcagagaacc gtttctttgt cagagtgtca ggtacagata aggcatctgt ggaaaaagta
      661 tttggaaatg tagaatatat tgatgcacag ggtgttacag gagagcttgg ttttgtgacc
      721 gaagtgatga cagaagaagc tttggagaac aaaaaagcag catatgggga agtgttgaat
      781 gtcattcgtg tagcatagaa aaaagagcag atcgtggatc acatggaagg cggttcacgg
      841 gtctgctctt tttatgaaaa cggaagagtg tgaaaagtaa gattgtttgc acatagattt
      901 gtgcttacac acgaatttga gatacgcaaa agcagcgtgg aaatggagag gaatatgacg
      961 ttaagtgaat gttacgaaga aatgggcgcg gatctgcacg aagtgctgcg gcggctaaag
     1021 acggaggaga ggatcgggaa atttttaaaa ctgattctga cggatacgaa ttatgagtcg
     1081 ctgtgtaagg cgctggaaga aaagaattat gagcaggcgt ttacacatat acataattta
     1141 aagggactgg caatgaatct gggactgacg ccgcttttgg ttccggcgca ggagttgtgt
     1201 gaggaactta gggatggtga accggaaagg gatcttaaac cgcttttgga tgaggtggcg
     1261 gcggcttatc agaaagtcct tggtattgtc gaaagacttg actgagataa cagtgtgaaa
     1321 agctggaatt ttggctgatt ctttgtaatg agttattcgg aaatggagaa cagtatcaaa
     1381 ggtaaagatg catttttgaa tctctgacat atgataaaac agaaggtttt atgtctgagg
     1441 tagaaaaaag tgcaggtaat tcttttgatc atagcagtca gtgtggatgt ctttgtggca
     1501 tgtgtggcgt gtgggagtga gcagataaag atcggatctg cggcagcgtt atgcatcagc
     1561 acggtctgca gtggaatatt agggatatca ctttatgcgg ggatggcgtt acagggggta
     1621 cttttaaaaa aatatgcatt atacttaagt tttttcggac tgcttgcagt gggaattttt
     1681 aagctgacag agtatgcgat cagaaaatat attgagcgtc ataagttcct ttgcaagaaa
     1741 gtcaaaatct ctttttcaca gctcagtttt attctgagta tctacaacaa tccggtaatg
     1801 gcagaccgcg atcactcggc gacgatgtcg tgtgtggagg ctgtattttt tgcacttgca
     1861 atgtcattgg acgggatgtt tggaggactt ggtgcaggat ttaccggaaa gaatatttat
     1921 tttacagtga tgggaagttt tgttgtcagt ttctgtgccg tactttgcgg atgcaggatt
     1981 ggcatgcagg catctaagcg gtggaagaca gatgtatcat ggcttggcgg cgtgttgttt
     2041 gtaatacttg cgtttcagag gattatttaa aaagtcagtt ccatgctgcc cattgctgca
     2101 ggaaaatcag aaaagtctgt tactgtttat gccttgccgg gcgtttgggt aataaagatt
     2161 ttctgcgtat gggcggcaca tactgccgct ttctatggat ttggaaataa tgtataaata
     2221 tacataaata tgcataaaat cttagtgcta tttgttttag gaaaaaggtt gaaaaatatc
     2281 ttatgatatg atacactttt tacaatttag gaaaaagact ataggtacta ataagggaaa
     2341 gttcagtcag gggactgtct ggatggaaaa agaagaaaaa cggcgacgtt tttttttttg
     2401 gaaaaaaagt gaaaggacag ggaaacgaat gaaagctcat ttaatactcg aagatgggaa
     2461 tgtatttacc ggaacaagta taggttctac tagggaagtc atcagtgaga tcgtatttaa
     2521 tacgtcgatg acaggttatc tggaggttct gactgatccg tcatatgcgg gacaggcggt
     2581 tgttatgact tatccgctga ttggaaatta tggaatcaca ccggatatgg agtcaggcag
     2641 accgtggccg gatggatata tcgtaaggga gttatcaaga atgccgagta acttccgtga
     2701 ggaggggaca attcaggatt tcctcacgaa atatgacatt ccgggtatag cagggatcga
     2761 cacccgtgca ctgaccaaga tccttcgtga gaagggaacc atgaatggta tgatcaccac
     2821 gaataataac tataatatag aagaaattat tcccagatta aaagcataca cgacaggtaa
     2881 tgttgtggac aaagttacct gcagtgaaaa acgagtatta aaaggaaatg gcaagcgagt
     2941 tgctttgctt gaccttggag caaaaaataa tattgcccag tctttaaata aacgcggctg
     3001 tgaagtgacg atctatccgg cacatacgac tgcagaagag atccttgcaa caaatccgga
     3061 tggaatcatg ttaagtaacg gacctggaga tccgaaggag tgcaaggaga tcattgctga
     3121 gatcaaaaaa ttatatgact ccgatacacc aatctttgca atctgcttag gacatcagct
     3181 gatggcactt gcaaccggcg ctgacacaca taagatgaaa tacggacacc gtggcggcaa
     3241 ccatccggta aaagatttag aaaccggaag agtatatatt tcctcccaga atcacggtta
     3301 tgtggtagat acggatacac ttgatccaaa gatcgcgaaa ccggcatttg tgaatgtaaa
     3361 cgatggaacc aatgagggac tttcctatgt aggcaaaaac atttttaccg tacagttcca
     3421 tccggaagcg tgtccgggac cgcaggattc tgcatacctg tttgatcgtt ttatttcgat
     3481 gatgtagaaa aattcttacg ttattgttta acaaatttta ggaggaagaa ttaatgccaa
     3541 gaaatcatga cataaaaaaa gttttagtaa ttggttccgg cccgatcgta atcggacagg
     3601 ctgcagagtt tgactatgca ggtacacagg catgccgctc attaaaagaa gaaggcgttg
     3661 aggttgtgct ggtaaactca aacccggcga cgatcatgac agataaagat attgcagatc
     3721 aggtttacat tgagccgctt acggtatccg tcttagagaa gattatcgca aaagaaaaac
     3781 cggacagcgt actgccgaca ttaggtggac aggctggatt aaatctcggt atggaattag
     3841 aggaaaaagg catccttgaa aaatataatg tcagactgat cggtacaaca gcagaaacga
     3901 tttttaaggc agaggaccgt caggcattca aagataccat ggaaaagatc ggtgagcctt
     3961 gtgcagcatc ccaggttgta cacaacgtac aagatggtat caagtttacc aataccattg
     4021 gttatccggt cgtacttcgt ccggcatata cgcttggcgg aagcggcggt ggtatcgcac
     4081 ataacgagac cgagctggtt gagatcttaa caaacggtct gcgtctttcc cgtgtcggag
     4141 aggtattggt agagcgttgt atcgcaggat ggaaagagat cgagtatgag gtaatgcgtg
     4201 atgcgaacgg caactgtatc accgtgtgta acatggaaaa tatcgatccg gtcggtgtac
     4261 ataccggtga ttccatcgtt gttgcacctt cccagacact tggcgataag gaataccaga
     4321 tgctccgtac atctgcatta aatattattt ccgagttaaa tatcaccgga ggatgtaacg
     4381 tacagtatgc attgaatccg gacagctttg agtactgcgt catcgaggtt aacccgcgtg
     4441 tatcccgttc ttctgcactt gcatccaagg caaccggata tccgattgca aaagttgccg
     4501 caaagatcgc attgggctat accttagacg aaattaaaaa tgcaattacc ggaaaaactt
     4561 acgcaagttt cgagccgatg cttgactatt gtgttgtaaa gattccgaga cttccatttg
     4621 ataaattcat cacggcaaag agaaccctga ccacacagat gaaggcaacc ggagaggtta
     4681 tgagtatctg caataacttt gagggtgcat tgatgaaagc aatccgttcc ttagagcagc
     4741 atgtggacag tttaatgtct tacgacttta ccggactttc cgataaagag ttagatgagc
     4801 agctgcatgt agtggatgac cgccgtatct gggtgatcgc agaggcactg cgcagaggcg
     4861 taagctatga caagatccat gagatcacga agatcgaccg ctggtttatt gacaaacttg
     4921 ccatcctggt tgagatggaa aaccagttaa agaatgagcc tttgactgta gaattattaa
     4981 aagaagcaaa acgcatcgag ttcccggaca gcgttattgc aaaactgacc ggaaagaccg
     5041 aaaaagagat ccatgacatg cgttatgaaa atggtatcgt ggcagcattc aagatggtag
     5101 atacctgtgc cgcagagttt gaggcatcca caccgtacta ctattccgta ttcggaagcg
     5161 agaacgaagc cattgagaca aatccgaaga aaaaagtact tgtacttggt tccggaccta
     5221 tccgtatcgg acagggtatc gagtttgatt actgctccgt acactgtacc tgggcatttg
     5281 caaaagaggg atgggagacc gtcatcatca acaacaaccc ggagaccgtt tccactgact
     5341 ttgatatcgc agacaaactt tattttgaac ctttaacacc ggaagatgta gagagcatcg
     5401 taaaactgga aaaaccggat ggtgctgtcg tacagtttgg tggacagacc gcgatcaagt
     5461 taaccgagag cctgatgaag atgggtgtta agatccttgg aacaaaagca gaggatgtcg
     5521 atgcggcaga ggaccgtgag cttttcgatg agattctgga aaaaacacag attccgagag
     5581 ctgcaggtgg aactgtattt accgcagagg aggcaaaaga agttgcaaac cgtctcggct
     5641 atccggtact ggtaagacct tcctatgtac tcggtggaca gggtatgaag atcgcattca
     5701 acgatgatga gatcgaggaa tttatcggca tcatcaatcg tatcgcacag gatcacccga
     5761 ttctcgtaga taaatattta cagggaaaag aaatcgaggt cgatgctgta tgtgacggaa
     5821 ccgatatctt aattcctggt atcatggagc atatcgaacg taccggtgta cactctggag
     5881 acagtatttc cgtatacccg gcaccgacga tcagtgacca tgtaaaagag acaatcgttg
     5941 agtatacgaa acgtctggcg caggcactgc atgtcatcgg actgatcaat atccagttca
     6001 tcgctatgga cgagcaggta tatgtcattg aggtaaaccc gcgttcttcg agaaccgtgc
     6061 catacatcag caaagtaacc ggtattccga tcgttgacct tgcaacgaaa gtaattatcg
     6121 gtgatactat ccgcggactt ggctatgagc cgggacttgc accgaccgca gattacattg
     6181 caatcaagat gccggtattc tccttcgaga aactgcgtgg cgctgagatc tccttaggac
     6241 cggagatgaa atcaaccggt gagtgccttg gtattgcaaa gaccttcaac gaggctctct
     6301 acaaagcatt cctcggcgca ggcgtgactt taccgaaata caaacagatg atcatgaccg
     6361 tcaaagatgc agataagccg gaagcagtcg gtgttgcaaa acgttttgag gcacttggct
     6421 acaagattta cgcaaccaga agtaccgcaa aatatttaca ggagcacggc gtcaatgcac
     6481 ttcgtgtcaa caagatctct caggagtcac cgaacgttat ggacttaatc ttaggacata
     6541 agatcgacct tgtcatcgac acaccgacac agggaaatgg tgataagacc agagacggat
     6601 tcctgatccg gagaaatgca atcgagacag gtgtttactg tatcacagca atggatacgg
     6661 caaatgcgtt ggcacgcagc ttagagactg cgaccgatga tctgacaccg gtggatattg
     6721 cgaccgtaaa gaacttgtag ttctttgaat ttataattct ttgaacttgt agttctttgg
     6781 atttataatt ctttaaactt gtagttcttt gaatcttata atatagaaaa gacagcacaa
     6841 atccaaaagg aggcgtgctg tttttgcttt tgcggaatat ggatattggc gacaacatga
     6901 aaaaacgaca tgaaagcagt taagaaattg ctttcatgtc gttttttata tcgccggaat
     6961 atccactcca acatatagaa aaaagtcgcg cggattaacg gagtttcatt tttttgcgcg
     7021 gtttcttttg ggtcagtggt ttttctgtgc ccaaagcatc ctgcgggatc acagatgcac
     7081 tactcattaa ggcgcttgcc gctttttgtt ccagtttact ttgattgtgc tgtgcaatct
     7141 gaagcttgac cttgcggaaa cggtctgccg tttctttgat gtaggcatcg gaaacaaaat
     7201 gctttacaga atctttatcc aatgcgatac ggttgtccat ggttttcatg atatctgtga
     7261 tctttaaaga tttgttgatc accgggtcac ttaaatcgta tatcatgttt gtggaaaact
     7321 tcagtaccat cggatgaaga aaatcagccg gattttcggc gagcacaaga gctttttctc
     7381 catcggaaag atcaacacat gcaccggtcg gaagaatatg gatacattct gctaaagcgg
     7441 aaagtacgag cggattgtag gtggctttat cctttttcag acgtcccatg gcagcaattt
     7501 ctgaaattgg cggtttgtta atgttcattg cggtcagatg gtcaaattcg tccgctactg
     7561 ctaaaatatc gacgagaaga cggatattgt ctgccggttc tttgactttt aaagtacggc
     7621 tgtttttaaa aatgatctgc tggataatat caagcgtatg taccgggaat ttataatcac
     7681 cacggtccgg gcggagtgtt tcataaccgc gctctaagtt gagctggata aaatccttat
     7741 ctgctgttgt aaggtcatcc cctttgtcta acactgtctg tggtacatac agataaccaa
     7801 aatcgtagag aagcgcagct gtgataaggg ctgtctgttc tgcagcaggc agatgcatct
     7861 gatttgtgat catcgcacag agaatgccga cactgatgga atgtttgtaa acaaaatctg
     7921 cagaacttcg caggttttgt gcaaaattta atttgtgatc aagactgccg taatgtgtct
     7981 ggatgtctct gacgagatcg ctcagcgaaa caggtgcttg attggcctgc agacgatcca
     8041 tattttcttt taatttaaaa acatatatag tctggaactg ttcgaattca agatcctctc
     8101 tggaaagagg cggtaccggc tctgccggct caagaataaa aataccgatc agaccaaagt
     8161 tttcgatact gttaataccc tgcatggtta aaagtgtgtc tctttcataa agaagaacac
     8221 ccatcttatt gtagattggt tttgcaagtc tcatgccggg ttttaaatct gctgttttta
     8281 caaattgcat aggtaaatcc tccccctgtg atgattatca ggctcttttt ataccatatc
     8341 cagacatcag atgcctgaat attcccccaa taatggtatg tgaaaaaatt aatattcata
     8401 ttgtgcttta catgctataa taccatatta atagaagaaa tacaatgaac gaaaaataca
     8461 acagtagcag aaagcttact gttcagccga aaggaacagc atgagacaca attttttgag
     8521 aagaatcctg ggaggaatac tgaccggctg catacttgtt acggcaactg tccatgcagg
     8581 aacgacacag aaagatattg ataaggcaaa agaccagatc acagacctta agaaacagaa
     8641 agaagatgcg aaagatcagg tggacagcct gaccaatgaa aaacagggat tggagagcga
     8701 ccttagcgga ctgaacggac agttaaatac gatcacttct gatatgaacc agttagagca
     8761 gcaggcagcg cagaaagaag acgagatcaa tacggcggcg gaagatctga aacgttccga
     8821 ggaacgatct gcagaacagt atgaagatat gaaagtgcgc attcagtata tttatgaaaa
     8881 tggcaatcag tctgtatggg acatgcttat tcatgtgtcc tccatcgcag aatttttaaa
     8941 tcaggcggaa tatacaagtg caatcaatca gtatgacaga aatatgctta cggcatatca
     9001 ggagctcacg gagcagatca aaaaacagaa aaaggaactc gaggagcaga aggaagaact
     9061 gactgtatta cagcaggaaa aacagaaaga gcaggaaaaa gtcaacagcc tgatcgagga
     9121 tacgcagaag aagatacagg aatccagcga gaaaattgaa gatgcacagt ccgatgtgga
     9181 atcgatcgat gcaaagatcg agaaaatgat cgaatacgaa aagcagcttg agatacagaa
     9241 ggcaaaagag gatgctgcac gccttgcagc catcaaaaag caggaacagg aaaacaggtc
     9301 gaatgtgaca tacgcaccgg cacagagcga tgcgtatctg ttagctgcca tcatccagtg
     9361 tgaggcatgg ggcgagccat atgatggaaa acttgcggtg ggcagcgtga tcatgaaccg
     9421 tgtcagaagc tcctattttc cgaatacggt ttccggtgtg atttatcagg gagggcagtt
     9481 ctctccggtt gccagcggtc gtcttgcata ccgtctggag cagggagtcg cttccgattg
     9541 tatgagtgcg gcacaggcgg ttctgggtgg aacgagcaca acaaattgtc tgtattttcg
     9601 tgtgaataat ggcattatag aaggaacagt tataggaaat catgtgtttt attaaacaaa
     9661 atgataaaat gataaaaatt taacaaaaaa ccccgcaaaa tggagcaaaa aggatgtaaa
     9721 aaattgttta caaagatgaa aaataaagaa agctattgca attaattgtg aaatatgtta
     9781 gcataagaaa gtacaagaaa tgcagaaaaa tagctgatta catagcaaat aagcttttta
     9841 aaatgaaaga aaaaaagata aatttcaacc gaaagtttga atttattttt ttaaaaaaga
     9901 ttgttaaaaa attaacagca tttttcggca actttaaaga gaaagaaaca aattctctgc
     9961 aaaagcagag ctttgtgtta aattgaaagg agaattcaaa atggcaaaga aagtagtttt
    10021 agcaggtgcc tgccgtaccg caatcggtaa aatgggtgga gcattaagca atactccagc
    10081 agcagactta ggtgcaatcg ttatcaaaga ggctttaaga agagcaaacg ttaaaccgga
    10141 gatggttgac gaggtaaaaa tgggttgtgt aatccaggca gctcagggac agaacgttgc
    10201 acgtcaggca gcaatcaaag caggactgcc aatcgaagtt ccggcaatca caatcaacgt
    10261 tgtttgtggt tctggtttaa aatgtgtcaa tgatgcagct accatgatca tggcaggtga
    10321 agctgatatc gttgttgcag gtggtatgga aaacatgtct atggcaccat atgctatgac
    10381 aaaagctcgt tttggatatc gtatgaacaa tgcaacgatc atcgatacaa tggtaaatga
    10441 tgcattaaca gatgcattca accactatca tatgatgatc actgcagaga acgtatgtga
    10501 taaatatggc attacaagag aagaacttga cgagttctca gcaaacagcc agcagaaatg
    10561 tgaaaaagca atcgctgaag gcaaatttga tgatgagatc gttccggttc ctgttaaagt
    10621 gaaaaaagag atcgttgatt tcgtgaaaga tgagggacca cgtgccggta caactgctga
    10681 gtctttagca aaattaaaat gctgctccgg taaagagggc ggacttgtta cagcaggtaa
    10741 cgcttccggt atcaacgatg gtgcagcagc aatcgttgtt atgagtgaag aaaaagcaaa
    10801 agagcttggt gttacaccaa tggctacatg ggtagcaggt gctcttggcg gagttgagcc
    10861 agagatcatg ggtgttggtc ctgttgcttc tacaagaaaa gtattagcta aaacaggttt
    10921 atccattgat gatatggact taatcgaagc aaacgaagca ttcgcagctc agtctatcgc
    10981 agtagcaaga gatctgaaat tcgatatgtc aaaagtaaac gtaaacggtg gtgcaatcgc
    11041 attaggacac ccggtaggtg cttccggatg ccgtatcctt gttacattac tgcatgagat
    11101 gcagaagaga gacgctaaga gaggtcttgc tacactttgc atcggcggtg gtatgggatg
    11161 ctctacaatc gttgagagat aatagttttt tatagtaatt cccggggaat gacttcccgg
    11221 ggattgcatt tttcaaacat acagttaaca taattatttt caggaggtaa ccgaaatgaa
    11281 ggttggcgtt attggtgcag gaactatggg acagggcatt gcaaaagcat ttgctcaggt
    11341 agaaggatat gaagtagcac tttgcgatat caagcaggag tgggctgaag gcggaaaaga
    11401 gaagatcaaa aaaggttatg caagattagt agaaaaagga aaaatgacac aggaagctgt
    11461 tgatggcatc cttgcaaaaa tcactccagg tttaaaagaa gacctttgca aagactgtga
    11521 tctgatcgta gaggcagctt tcgagaatat ggaagtaaag aaaactactt tcaaagaatt
    11581 agacgagatc gcaaaaccag attgtatctt cgcttccaac acatccagtc tttctattac
    11641 agagattgga aacggattaa accgtccaat ggttggtatg catttcttca acccggcaga
    11701 ccgtatgaaa ctgatcgagg ttatcgctgg tgtgaatact ccggatgaaa ctgtttccgc
    11761 tattaagaaa atttctgaag agatcggaaa aactccggta caggttaatg aggctgctgg
    11821 tttcgttgta aaccgtatct taatcccaat gatcaacgaa gctgcattca tcaaaatgga
    11881 aggtgtttcc gatatcgctg gtatcgatgc agctatgaaa cttggtgcta accacccaat
    11941 gggaccgtta gagttaggtg atttcatcgg tcttgatatc tgccttgcta tcatggatgt
    12001 actttacaaa gagacaggcg acagcaaata tcgtgcatgc ccactgatcc gtaagatggt
    12061 tcgtggtggt aaccttggag caaagagcgg caagggattc tatgtataca atgctgatcg
    12121 tacaaagact ccggtcgatg ctcagtaaaa taaatattta tggaggaaaa ttagatcatg
    12181 gatttcactt tagacaagaa acatgaaatg gcgcgttcct tattcaagga atttgctgaa
    12241 acagaagtaa aaccgcttgc acaggaaaca gatgagacag aagctttccc ggctgaaacg
    12301 gttaagaaaa tgcagaaata cggattcatg ggaatcccgg ttccgaaaga atatggtgga
    12361 cagggttgtg atcctcttac atacgttatg tgcgtagaag agttatctaa agtttgcgca
    12421 accacaggtg ttgttgtatc tgcacataca tctctctgca tcgatccgat catgacatat
    12481 ggtacagaag agcagaaaca gaaatatgta aaaccacttg caacaggcga gaagttaggt
    12541 gcattcgcat taactgagcc gggtgctggt actgatgcac agggtgcaca gacaaaagct
    12601 gttttagatg gtgacgagtg ggtattaaat ggatcaaaat gcttcattac caatggtaaa
    12661 gttgcagatg tttatattgt aatcgctatc acaagcatca cagaggacaa gagaggcaga
    12721 aagaagaaaa acttctctgc atttatcgta gataagggag ctcctggttt ctcattcgga
    12781 acaaaagaga agaagatggg tatccgtgga tcatctactt atgagctgat cttcgaagac
    12841 tgcagaatcc cgaaagagaa tctgttagga ccggaaggaa aaggtttccc gatcgcaatg
    12901 cacacacttg atggtggacg tatcggtatt gctgctcagg cacttggtat cgctgagggc
    12961 gcattagaca gagctatcgc ttatacaaaa gagagaaaac agtttggacg ttcaatcgca
    13021 cagcagcaga atacacagtt caaacttgct gatatggctg caagaatcga agctgcacag
    13081 cttttagttt acaaagcagc aatggcaaaa gctactcaga aagtatattc tgtagaagct
    13141 gcaaaagcaa aattattcgc agcagagact gcaatggctg ttacaactga ggttgttcag
    13201 ttattcggtg gatacggata tatcagagag tacgatgtag agcgtatgat gcgtgatgct
    13261 aagatcactg agatctacga gggtacaagc gaagtccagc gtatggtaat ctccggtgca
    13321 ctgttaaagt aatttcgaat caatccgcgt aaaatttaaa catataaaac ataaggagtg
    13381 aaaaatacat gaatatcgta gtatgtatta aacaggttcc tgatacaaag ggcggcgtta
    13441 agttcaaccc ggatggaacg cttgacagag cagcaatgct tgctatcatg aacccagatg
    13501 ataaagctgg tcttgaagca gcacttagaa tcaaagatga gacaggagca aaagttacag
    13561 ttcttaccat gggacttcca aaggctgatg cagttcttcg tgaagcatta gcaatgggtg
    13621 ctgatgaagc agttcttgta acagaccgcg tattaggtgg agctgataca tgggcaacat
    13681 ctaccacaat cgctggtgca cttcgtaact tagactatga cttgatcatc acaggacgtc
    13741 aggctatcga tggtgatact gcacaggttg gtcctcagat cgctgagcat cttggacttc
    13801 cggttatctc ttacgcagca gacatcaaag tagagggtga ttctgttgtt gtaaaacgtc
    13861 agtacgaaga cagatatcat gagttaaaag caaaaatgcc ttgtctggta actgctcttt
    13921 ctgaattaaa tgagccgcgt tacatgacac cgggcggaat cttcgatgca tttgacaaag
    13981 aagtaaccgt ttggggcaga gcagacttaa aggatgttga cgattctgat cttggtttaa
    14041 agggatctcc tactaagatt gcaaaagcat ctgataaagt tgcaaagggt gctggtgaga
    14101 aagtaaacct tgatccggca gaatccgtag catatttgat cggtaaattc aaagagaaac
    14161 acattatcta ataaaaaaag gaaaagtggt gaataacatg gcattagaag aatataaagg
    14221 agtatttgtc tttgcacagc aggtagataa cgtattagat ggcgttgcat ttgagttact
    14281 tggaaaaggc aaagacttag caaaagattt aggtacagac gtaactgctg tattaattgg
    14341 ttctggtgta aaaggattag cagatcagtt agctgagtat ggtgctgaca aagttatcgt
    14401 agtagatgat cctgaattaa aagactacag aacagagccg tatgctcatg cattagcatc
    14461 tgtaattaac gagttcaaac ctgaaatcat gttagttggt gcaactgcta tcggccgtga
    14521 cttaggacct actgtttctg caagagtaaa aacaggtctg acagcagact gtacatctct
    14581 tgagatcggt gatttcccac tggttgctgc acctggtaaa gaatcagagc agaaacacaa
    14641 ccagttatta atgacacgtc ctgcatttgg tggtaacacc atcgcaacaa ttgcctgccc
    14701 ggacaaccgt cctcagatgg caactgtacg tccaggtgtt atgcagaaga tcgctccgat
    14761 cagcggtgca aaagcaaacg ttgtagagta caacccggga tttacaccaa acaacagata
    14821 tgttgagatc ttaaacatcg taaaagctgt taagagcaca gcaaacatta tggaagcaaa
    14881 gatccttgtt tccggtggtc gtggagttgg ttccaaagag aacttcaaac ttttggaaga
    14941 tcttgcagag gtacttggcg gtactgtaag ctgctcccgt gcagttgtag agaatggctg
    15001 gttaccagtt gatttacagg taggtcagac tggtaaaact gttcgtcctc agatctactt
    15061 tgcaatcggt atttccggag caatccagca tgtagctggt atggaagatt ctgacctgat
    15121 catcgcaatc aacaaagatg aagatgctcc aatcttcgac gtagctgatt acg
//
