LOCUS       gnlProkkaNMIMCLMO_297   3373 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NMIMCLMO_297.
ACCESSION   gnlProkkaNMIMCLMO_297
VERSION     gnlProkkaNMIMCLMO_297
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-18 11:37:43
            Original ID  :: gnl|Prokka|NMIMCLMO_297
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3373
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3373
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1:2587]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2..2587
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..3373
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3373
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     CDS             complement(2..2587)
                     /NRPS_PKS="Domain: AMP-binding (20-419). E-value: 1.1e-105.
                     Score: 345.4. Matches aSDomain:
                     nrpspksdomains_ctg297_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (521-585). E-value: 3e-12.
                     Score: 38.8. Matches aSDomain:
                     nrpspksdomains_ctg297_1_PP-binding.1"
                     /NRPS_PKS="Domain: Condensation_LCL (597-861). E-value:
                     1.4e-34. Score: 111.4. Matches aSDomain:
                     nrpspksdomains_ctg297_1_Condensation_LCL.1"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     313.4; E-value: 3.7e-95)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg297_1"
                     /sec_met_domain="AMP-binding (E-value: 3.4e-110, bitscore:
                     355.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-13, bitscore:
                     38.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.1e-45, bitscore:
                     142.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNINERNNFVFEFRPVTELFEEQVKLHPEKCAVVSGKESFTYAQ
                     LNERANRIANALVEKGVRRETIVGVVLERCCDFYAVRQGILKAGGAFAVATPDYPDDRI
                     RYIFEDSGAPFIITTKEIAEERRELFAKLPCTVLLIEELLENENAENPQVKIGEHDLCY
                     CIYTSGSTGKPKGVLIEHINLANFVNPDPKNAETYGYVSRGSVSLSMAAMTFDVSVLEE
                     FLPLTNGMTAVIASDEEILNPLMLGELIVRNKVDIMTTTPTYLSNMIDLPQLEKAVSQI
                     KVFDVGAEAFPPALYDKIRRVNPDAYIMNGYGPTETTISCTMKVITDSRNITIGTPNGN
                     VKVYIVDKENKILPDGETGELVIAGLGVGRGYMNLPDKTEAVFIDLNGERAYKTGDLAR
                     ISPEGEIEFFGRIDNQIKLRGLRIELGEIEEVINSYEGIITSITLPVDNKFLCCYFMAD
                     RQINTEELSAYASESLAHYMVPEVFVQLEKMPVTQNGKIDKKALPKPAAQPKNLKEPQT
                     PMQKKIFEIVADVVENDFFGTDTSFYRAGLSSISAMKLCILISEEFGVTVKTSDIHENN
                     TVEKLEKYVMLAPKIRTYEKREVYPLTGSQKGIFAECMKNPESTVYNIPFLFELESSVD
                     VQKLSDAISQMIAAHPYLLTKVYLSDSGEMVQKPCEEAFVPEVVRTTNEQFEKIKDELV
                     RPFKLEKGRLFRAGIYVTEDRKYLFTDFHHILADGNSYDIIFEDIDRAYLGEKLEKESY
                     TGFDAALDEEQQMKEGKYKKAEKYYDSIFEGIETESLPLPDCSGKTPERGYLSMPVDTG
                     ESLVLSACEKLGVTPNILFTGVFGILMSRYSNSEESLFATIYNGRNDSRL"
     aSDomain        complement(5..796)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg297_1_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-34"
                     /label="ctg297_1_Condensation_LCL.1"
                     /locus_tag="ctg297_1"
                     /protein_end="861"
                     /protein_start="597"
                     /score="111.4"
                     /tool="antismash"
                     /translation="EVYPLTGSQKGIFAECMKNPESTVYNIPFLFELESSVDVQKLSDA
                     ISQMIAAHPYLLTKVYLSDSGEMVQKPCEEAFVPEVVRTTNEQFEKIKDELVRPFKLEK
                     GRLFRAGIYVTEDRKYLFTDFHHILADGNSYDIIFEDIDRAYLGEKLEKESYTGFDAAL
                     DEEQQMKEGKYKKAEKYYDSIFEGIETESLPLPDCSGKTPERGYLSMPVDTGESLVLSA
                     CEKLGVTPNILFTGVFGILMSRYSNSEESLFATIYNGRNDSR"
     CDS_motif       complement(359..424)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0006"
                     /evalue="7.90E-05"
                     /label="C3_LCL_132-143"
                     /locus_tag="ctg297_1"
                     /protein_end="743"
                     /protein_start="721"
                     /score="15.4"
                     /tool="antismash"
                     /translation="FHHILADGNSYDIIFEDIDRAY"
     CDS_motif       complement(635..730)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0005"
                     /evalue="2.30E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg297_1"
                     /protein_end="651"
                     /protein_start="619"
                     /score="20.0"
                     /tool="antismash"
                     /translation="TVYNIPFLFELESSVDVQKLSDAISQMIAAHP"
     aSDomain        complement(833..1024)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg297_1_PP-binding.1"
                     /evalue="3.00E-12"
                     /label="ctg297_1_PP-binding.1"
                     /locus_tag="ctg297_1"
                     /protein_end="585"
                     /protein_start="521"
                     /score="38.8"
                     /tool="antismash"
                     /translation="KIFEIVADVVENDFFGTDTSFYRAGLSSISAMKLCILISEEFGVT
                     VKTSDIHENNTVEKLEKYV"
     aSModule        complement(833..2527)
                     /complete
                     /domains="nrpspksdomains_ctg297_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg297_1_PP-binding.1"
                     /locus_tags="ctg297_1"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1301..1366)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0004"
                     /evalue="1.50E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg297_1"
                     /protein_end="429"
                     /protein_start="407"
                     /score="33.1"
                     /tool="antismash"
                     /translation="FFGRIDNQIKLRGLRIELGEIE"
     aSDomain        complement(1331..2527)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg297_1_AMP-binding.1"
                     /evalue="1.10E-105"
                     /label="ctg297_1_AMP-binding.1"
                     /locus_tag="ctg297_1"
                     /protein_end="419"
                     /protein_start="20"
                     /score="345.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEEQVKLHPEKCAVVSGKESFTYAQLNERANRIANALVEKGVRRE
                     TIVGVVLERCCDFYAVRQGILKAGGAFAVATPDYPDDRIRYIFEDSGAPFIITTKEIAE
                     ERRELFAKLPCTVLLIEELLENENAENPQVKIGEHDLCYCIYTSGSTGKPKGVLIEHIN
                     LANFVNPDPKNAETYGYVSRGSVSLSMAAMTFDVSVLEEFLPLTNGMTAVIASDEEILN
                     PLMLGELIVRNKVDIMTTTPTYLSNMIDLPQLEKAVSQIKVFDVGAEAFPPALYDKIRR
                     VNPDAYIMNGYGPTETTISCTMKVITDSRNITIGTPNGNVKVYIVDKENKILPDGETGE
                     LVIAGLGVGRGYMNLPDKTEAVFIDLNGERAYKTGDLARISPEGEIEFFGRIDNQIKLR
                     "
     CDS_motif       complement(1439..1528)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0003"
                     /evalue="7.30E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg297_1"
                     /protein_end="383"
                     /protein_start="353"
                     /score="37.5"
                     /tool="antismash"
                     /translation="PDGETGELVIAGLGVGRGYMNLPDKTEAVF"
     CDS_motif       complement(1625..1654)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0002"
                     /evalue="6.90E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg297_1"
                     /protein_end="321"
                     /protein_start="311"
                     /score="9.2"
                     /tool="antismash"
                     /translation="YGPTETTISC"
     CDS_motif       complement(2045..2104)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg297_1_0001"
                     /evalue="4.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg297_1"
                     /protein_end="181"
                     /protein_start="161"
                     /score="31.5"
                     /tool="antismash"
                     /translation="CYCIYTSGSTGKPKGVLIEH"
     CDS             complement(2601..3371)
                     /locus_tag="ctg297_2"
                     /transl_table=11
                     /translation="MIFVLIMLFTEEITVMFGSGGGELGSHMVQALRIFALCVAPYLWN
                     KFIISYYESIEETAIASFATFLENAVVVLPATLVGILVWKQIDGIGIDGIAAGFVATEI
                     ITAVAACIFRKIRHKNTSFYIVPDKNPGINLDFSIKSTMEEAQTVHKRIIEFCQEQGAS
                     KSKANLAAVCAEEMTVNIIRFGGKTSNWIDINLCLEDDLCRLRIRDNGVNFNPLEYQYD
                     SEDFDIHGIELVKKVSKSMDYIRAIDMNNTIISF"
ORIGIN
        1 ccagtctgga atcatttctt ccattataaa tagttgcaaa aagactctcc tcggaattgg
       61 aatatctgct catgagaatg ccgaatacac ctgtaaacag aatgtttgga gtaaccccca
      121 gcttctcgca ggcagaaagt accagagact ctccggtgtc cacaggcatc gacagatatc
      181 ctctctcagg agtctttcct gagcagtcag gaagaggcag ggattctgtc tcaattccct
      241 cgaaaatact gtcataatat ttctcggcct tcttatattt tccctctttc atctgctgtt
      301 cttcatcaag ggcagcatca aagcctgtgt aggattcttt ctccaatttc tcacccagat
      361 aggcccgatc aatatcctca aaaataatat cataggaatt accatctgcc aggatatggt
      421 ggaaatctgt aaaaagatat ttcctgtctt ctgtcacata aatccccgcc cggaacagcc
      481 gtcccttttc cagcttaaag ggacgcacca gctcatcttt tatcttttca aactgctcat
      541 ttgtggtccg gaccacctct ggtacaaagg cttcctcaca aggcttctgc accatctctc
      601 cgctgtcact taaataaacc tttgtcagca gatacggatg tgcagcgatc atctgagaaa
      661 tggcatctga cagcttctga acgtcaacag agctttccag ttcaaacagg aacggaatat
      721 tgtataccgt actctcagga ttcttcatgc actctgcaaa aatacccttc tgggagccgg
      781 taagagggta aacttctctc ttttcatagg ttctgatctt cggtgcaagc atcacatatt
      841 tttccagttt ctccaccgta ttgttttcgt ggatatcact ggttttcaca gtcactccga
      901 attcctctga aatcagaata caaagcttca tggcactgat agaagacagc cccgccctgt
      961 aaaaacttgt gtcagttcca aaaaaatcat tctccacaac gtctgcaacg atttcaaaaa
     1021 tcttcttctg cataggagtc tggggttctt tcagattctt tggctgtgca gccggctttg
     1081 gcagtgcttt cttatcaatt ttgccattct gtgtcacagg catcttttcc agttggacaa
     1141 aaacctcagg aaccatataa tgagccaggg actctgatgc ataggcagaa agctcctctg
     1201 tattgatctg gcggtcagcc ataaaatagc agcagaggaa tttgttatct acaggcagag
     1261 taatgctggt aatgattccc tcataggaat tgatcacttc ctcgatctca ccaagctcaa
     1321 tcctcagacc gcgaagcttg atctgattgt ctattcttcc gaaaaattca atctcaccct
     1381 cagggctgat ccttgcaagg tcaccggtct tataagctct ctctccgtta agatcaatga
     1441 atacagcctc tgttttatcc ggcagattca tataacctct gccaacgccc agtcctgcga
     1501 tcacaagctc tccggtctct ccgtccggaa ggatcttatt ctctttatct acaatatata
     1561 ccttcacatt accgttaggc gtaccaatgg taatgtttct gctgtccgta attaccttca
     1621 tggtacagct gatggtagtt tccgtaggtc cataaccatt cataatgtaa gcatccgggt
     1681 ttactctgcg aatcttgtca tacagagccg gaggaaaagc ctctgcacct acatcaaaca
     1741 ccttaatctg agaaactgct ttttcaagct ggggcagatc gatcatattg gacaggtaag
     1801 taggagttgt ggtcataata tccactttat ttctgacgat cagctcaccc agcataagag
     1861 gattcagaat ctcctcatca gaggcgatca ccgcagtcat cccgttggta agtggcagaa
     1921 attcctccag aacagacaca tcaaaggtca tggctgccat ggaaagagaa accgatcccc
     1981 tgctgacata accataagtt tccgcatttt tcggatccgg atttacaaaa ttggcaaggt
     2041 tgatatgttc gatcagcaca cccttgggct tgcctgtgga accagaagta tagatacaat
     2101 aacaaagatc atgctccccg atctttacct gaggattctc tgcattctca ttctccagaa
     2161 gttcctcgat cagaagtact gtacagggaa gcttagcaaa cagttccctt cgttcctctg
     2221 cgatctcctt tgttgtgata ataaaaggag ctcctgaatc ctcaaaaata taccggatcc
     2281 tgtcatcagg ataatccggt gtggcaactg caaatgcgcc tcctgccttc aggataccct
     2341 gtcgcactgc atagaaatca cagcatctct caagaaccac acctacgatg gtctcccgtc
     2401 tgacaccttt ttccacaagt gcattggcaa tcctgttagc ccgttcgttg agctgtgcat
     2461 aggtgaagct ctctttgccg gagacaactg cacatttctc tggatgaagc ttcacctgtt
     2521 cctcgaacaa ttctgttacc ggccgaaact caaaaacaaa attgttcctt tcattaatat
     2581 ttttcatttt catgtacctc ctaaaaagat attatggtat tattcatatc aatcgcacga
     2641 atataatcca tggattttga taccttcttg acaagttcaa tcccatgtat atcaaagtct
     2701 tcactgtcat actgatattc cagagggttg aaatttacac cgttatctct gatcctcagt
     2761 ctgcagagat catcctccag acagaggtta atgtcgatcc agttggaggt ttttcccccg
     2821 aatcgtataa tatttacagt catttcctca gcacacactg cggcaagatt tgccttgctt
     2881 ttagaagctc cctgttcctg gcagaactct atgatcctct tatgaaccgt ttgtgcttcc
     2941 tccatggtac tcttaatgga aaaatccaga ttaatccccg gatttttgtc aggaacaata
     3001 taaaagcttg tattcttatg cctgattttt ctgaaaatac aggctgcaac tgcagttatg
     3061 atctcagtgg caacaaatcc tgctgcaatt ccgtcgatac cgatcccgtc aatctgtttc
     3121 catacaagga ttcccaccag ggttgcaggc agaactacca ccgcattctc cagaaatgtg
     3181 gcaaaacttg caatggctgt ctcctcaatg gactcgtaat agctgataat aaacttattc
     3241 cacagataag gtgccacaca caaagcaaag atgcgcagcg cctggaccat atggctgccc
     3301 agctcgccac cgccgcttcc gaacatgaca gtgatttcct cggtaaacag cataataagg
     3361 acaaagatca cag
//
