LOCUS       c00216_gnlPro..         7063 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OMOMMHNM_216.
ACCESSION   c00216_gnlPro..
VERSION     c00216_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:32:03
            Original ID  :: gnl|Prokka|OMOMMHNM_216
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7063
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..93
                     /locus_tag="ctg216_1"
                     /transl_table=11
                     /translation="SRGFVRKKQKVCILTHLRFFEEEKGKGEGR"
     protocluster    1..7063
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4002:6975]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4003..6975
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7063
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7063
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(225..2342)
                     /locus_tag="ctg216_2"
                     /transl_table=11
                     /translation="MEHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPIFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(2430..2660)
                     /locus_tag="ctg216_3"
                     /transl_table=11
                     /translation="MYYPDQPFHICTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             2854..3357
                     /locus_tag="ctg216_4"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             3378..3545
                     /locus_tag="ctg216_5"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             3532..3975
                     /locus_tag="ctg216_6"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVNELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(4003..4731)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_7"
                     /sec_met_domain="Radical_SAM (E-value: 3.4e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4747..6975)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_8"
                     /sec_met_domain="Gly_radical (E-value: 3.9e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             complement(6987..7061)
                     /locus_tag="ctg216_9"
                     /transl_table=11
                     /translation="HIYADYMKKRCNFVRSKEKDNYNL"
ORIGIN
        1 agtcggggct ttgtgcgtaa aaaacagaag gtgtgcattt tgacacacct tcgttttttt
       61 gaggaagaaa aaggaaaagg tgagggtagg tgagggtaaa tttctaacat tcgtattttt
      121 ttttagaaga aaatagaaga atagaaataa gaaatataaa aatgaaaagt tgcaaaagta
      181 ccctcaccaa ccctcacctt tttgcgtgcg aaggagatga aatcttattc atatagcctg
      241 tttgatttca cctgttccgt atcaataatg aatacagtgt atcttctccc gttttttccc
      301 ttatgaaatt cgaagccgtg tttggacatc acttttccta gttgggcttt ggttccgtga
      361 gaatattgat accccgttcg ttcgcgaatc aattccgcaa tttcagtgac cgtcagataa
      421 tgcacctttt cgaaacgttc cggtttgcgg atatgtgtca acaccagttc ttcttccggt
      481 gagtggaaaa tgaaatcctc gttgttctct tcaatccgtg agttttccga ttgggtgaac
      541 caatactgat accccggctt gttgagcaga tacttgattt gtgcatacaa ctgcgcgtag
      601 tttattttga taaactccat cgagttgaca tgaaagcaga gaaaacggcg gttacctgtg
      661 gtgtcgtgaa gcacttcctg ataattgcat gtgccggcaa aagaagccgt gtgagggaag
      721 ttctgtgaac gccgcgcgta tggcaggcgg atgctgatta ctttgcgggt aaccaaatcc
      781 ttgaagatat tcagttcccg tccgttcatc ccttcaaact catccagatt gataagcatt
      841 gcctgcgcta ttttggccag atcatccttg ttgttcgggt taataagacc ggtagaaaga
      901 taggcgcgca attcgggcgg cagcaggttg ttgataaacg tagtctttcc gatgttctgt
      961 atttcgctgc aaagcaacaa aacagtgtgg ttcaccacat cgtcttgcgt ggcggcagcg
     1021 cacattccca ccagataacg ttccagacat tctacccaaa atttctggtg actagtatgc
     1081 acgctgttgg caaggatacc gatataatcc gttccgtccc attcaggcag cgagtctaaa
     1141 tactcgcgaa taggatggta ggtgctgctg aagtcggagt agatcagatt ctgaatcgtc
     1201 ttgacggtgc acgaatagcc cagttcgttc atctccatcc aaatgctgtt ttccatcatt
     1261 tcgtcgagaa tatggaaagg ttccggttgc tccgtgtccg ttttccttct gcgatattcc
     1321 atgatatgaa gcacttcgtt gtagcgtgta tcgtaatttt ctttgatatg ttgctcaatc
     1381 tgtaatattc gcttctgcgt actattcagc ttgcgggtgt cgaattcctc attgtgtccg
     1441 taagcactcc cgatcagtgc gtccatttca tccgcgggaa gatcggtgaa ctggctcttg
     1501 ataaaagctg ccgcctcttc ctgggggacg ccgtagcgat tgtacataca agccaggcaa
     1561 tgcaggtagt tgttccggtt gccggtgacg tacttttcgg atttattgtg ataatagttc
     1621 agagtcacca ttaaggaagc gtgcgaggaa tggtagttga gtgccacttg ttcggtgagg
     1681 ggattgttgt ctggtgctgt cgtctttttc tttttgccgg atgccctttt cttcttttga
     1741 gacttgaaga atatcggtgg ctgttccacg atgacagctg tggcatcggg attgaaataa
     1801 atatccggat cgtaagagaa caggcaagta cggctgacat cttgtccgga agtgtcgacc
     1861 tcaactccgc agagctgggc ataaaaggaa gcgattttgt gataagcggc atgatggaaa
     1921 tcttcaattt cctgaatcgt ttcgggcaat gtcccgtccg tccggcatac acgtaccacg
     1981 attttcagtc ccattccttt gggactgatg aaagcgatgc gggtgtgggc acactttttt
     2041 gccagtgcga ggatgcgtgc caatgcttcc tgtgtctgat ggtcgatgtc taccacgatg
     2101 tatcccagat acttgacgag agttgcgttt gaacgtcctc cccggtagtt ggctgaaaag
     2161 gtgatttccg gtagttctat tttcttttcg tttgctgctt tctcgccttg ttccgcgaag
     2221 attcggcgca cgtcctccac tgtttcccgg tatgtgccgt tgctgatggc cgatacgatt
     2281 tcttcttctg tcatttcggc tatgacgcgg taaaagtctt gaaagatggt aaaatgctcc
     2341 atgttggttt gttcttttta atttgcggtt gcaaagaaat ttgtttgtaa aataatagaa
     2401 tggaactttt cgtttttttg attaagttcc tagggttctc ccaaatagcg tatgatcgtt
     2461 gccacttgcc ggggagagag aaggcggttg cgggcacgat agccttcttg ttcgagttct
     2521 tgcaggagcg gaaggttgta gacaatccaa cgacgtagta tttgtaaggc tactttaagg
     2581 catacgttgg ggttgtaaag atgagctagt tccgactttt tataagtgca gatatgaaac
     2641 ggctgatccg gataatacat gatgaatgat atttggttta tgctataaag ttactgaaaa
     2701 ttaatggttt aataaaagaa taacgtcatt attattcata gagtatcagg aaacttcgtt
     2761 ggggaatccg gagggtgaaa aatgtgttat ctttatgtcg ttaacaaata aggaacgcgt
     2821 tcgaaatgtg tgaaatctta aaattatttt attatggcaa tgacagtaac ttattcagta
     2881 gtgccgcgca agaatcctgc caaaaaggat gagtcggcaa agtattacgc gcaggcacag
     2941 gcttcgggag agttggattt tgaagagttg tgcgagggaa tcaccagccg gtctacctgt
     3001 acggagacag atgtgcgtgc cgccatttcg ggtattcttt atgaggcgaa gcgtgcgttg
     3061 aaagcgggaa gaatagtccg gttgggtgat ttgggcagtt tgcagatcgg gttgaacagt
     3121 gaaggagctg tgtcggtcaa ggagttttca agttcgctga ttacggctgc ccatattatt
     3181 tttcgtcccg gaaagacgtt ggcggacatc acgaagattc ttagctacca gcaggtaacg
     3241 acgcgtgcgg tggcccaaac gggcggtagc ggcaatgaag gagaagatga tgacaaaggt
     3301 tccggtggcg gttcagacgg tggtggaagc ggagaagctc cggatccggc agcatgataa
     3361 ctttaatatt gaatcaaatg aaagaaattg taactaagat tttggatgta attatgttcc
     3421 ttgtgccttt cttcggaaaa agaaagcgga acagaattgt gagagaggtg cgcttcaatg
     3481 ctacccataa ggaagtgtgt aatgtgaaaa cgacggaaag ggagaaggac gatgagaaag
     3541 attagtctga ttgtgattca ctgctctgct acccgtgctg atcgcgactt cacggcgaag
     3601 gatgtggaca ctgcccatcg ctttcggggc ttttcttgtt gggggtatca ttattatatc
     3661 cgcaagtcgg gacagataga gccgatgagg gatgaagata cggtgggtgc tcatgcacgc
     3721 ggttttaatg cgattagttt aggagtgtgc tatgaaggcg ggctggatga gaacggaaag
     3781 gcagcagata cgcgtacttc ccgccagaaa gaggcgatgc atcgtctggt aaacgagttg
     3841 ttgcagcgtt atccggatgc aaaggtggtc ggtcatcggg acttgagtcc tgacaccaat
     3901 tataatggaa ttgttgaccc ttgggagcgg atcaaagagt gtccctgttt cgaggtgaaa
     3961 gcagagacat ggtgattttt atcctattta ttactgtggt ggttaattga ccaccacagt
     4021 agtgaaatac tctttaaata cctccgcggc tttttcaagt tgttcggggg tattttcttt
     4081 tacgtctttc agcttgtatt cctgctccat cgcttcatac ttatgtacgc ctaatgtatg
     4141 ataaggcagg atttccactc gttgcaccat cttatatttt cccagagcct ctcccaagcg
     4201 gcggatatcc tcttcgaaat cgctgtatcc gggcactaat acataacgca gccagaaagg
     4261 tttctcgttt tcttccagcc atgctgccgt gcggatggtt tgctcgttac ttcttccggt
     4321 cagtgcttga tggcgtgccg gattgaactc tttaatatcc agcaatacaa gatccgtcag
     4381 tttgaaaagt tcttctactt cttcgttcca gataccgccg ttgctgtcta tgcatacatg
     4441 tattcccttt tctttcagtt cacgcactaa agggactagt gctttggcct gaaacgtagg
     4501 ttctcctccg gagaaagtga ctcctccgcg tttcccgaaa aaaggacgtt ggctcatagc
     4561 catgcggacg atttcttccg gtggggtagg tgtacctcct tttccggcta tcgtgtcggg
     4621 attggcacaa tacaggcagc ggaaattgca tccttgaaga aaaacgacga gccgtaagcc
     4681 cggcccgtcg aatgttccca tactttcgta tgaatgtacg tttatcgtca tattgagtag
     4741 atctgattac atacgttcgt ggaagctacg gctgataact tccaattgat gttcgcggct
     4801 caacttcacg aagtttacgg cataaccgga aacacggatg gtgagttgcg gatatttttc
     4861 cggatgttcc atggcgtcgt aaagcatttc acggttcagt acgttcacgt tcaggtggtg
     4921 agcgcctttg gtgaagtaac catccatcat tgtcaccaga ttttctacgc gatcttcttc
     4981 agtagctccc agtgatttcg gaacgatgga gaaggtgttg ctgataccgt cctgtgagtc
     5041 gcggtaacgt aatttcgcta cggaactcaa agaggcgatg gcaccgttct tgtcacgtcc
     5101 gtgcataggg ttggctcccg gagcaaaagc gacacctttg gcgcggccgt cgggagtagc
     5161 acctgtcttc ttgccataca tcacgttgga agtgatggtg agcaaagaca gagtaggacg
     5221 ggcatttttg taaaccggaa gtttcttcaa ttcttcgctg aagaaataga ccaggtcaac
     5281 acccaggtgg tctaccttgt cgttgtcatt accgaagcaa gggaattcgc cttgaatatc
     5341 gaaaccttct gtcagaccga tgtcgttgcg gcgtgcggtt actttggcat atttgatggc
     5401 cgacagtgaa tcgagggcga tagaaagtcc ggctacaccg taagcaaggt tgatgcgtgg
     5461 gttggtatct acaagagcca tttgggcttt ttcgtagtaa tacttatcat gcatataatg
     5521 gatgatgttc atcgcttcgt tgtaaacacg ggcgatttct gtcaatacct tcttgtagtt
     5581 gctcattact tcttcgaagt tcagtgtgtc gctggttagt acggggatgt ttttcaccat
     5641 taccgtaccg gtgttttcgc aacgtccgcc gttgatggca agcagcaggg ctttagccag
     5701 gttgcaacgt gcgccgaaga actggatttg ttttccgatt tcctgataag atacgcagca
     5761 cgcgattccg tagtcatcag actggcgcac ttcacgcatc aggtcgtcgt tttcatattg
     5821 gatggaagaa gtgtcgatag atacttttgc gcagaattct ttgaatcctt ccggcagttc
     5881 cgggctccac aatacagtca ggttcggttc cggtgaaggg ccgaggttgt agagtgtttg
     5941 caggaagcgg aaagaagtct ttgtcacctt ggtgcgtccg tcgttgaggc gtccgcccag
     6001 ggattcggtt acccatgtcg ggtcgccggc aaagatgtcg ttgtaagatt gcatacgcag
     6061 gtggcgcacc atacgcagtt tgatgacaaa ctggtcgatc agctcctgtg cgaaagattc
     6121 tgtgatagtt cctttgctca attcatattc catataaatg tcgaggaacg aagagacgtt
     6181 acccagtgac atggcagcac cgtcttgttc ttttacggca gcaaggtaag ccatgtacac
     6241 ccattgtact gcttcttgtg cggtgtaggc aggacggctc aagtcgagtc cgtagtattc
     6301 gcccattact ttcatgtctt tcaatgcctt gatttgctct gccacttctt cgcgcaggcg
     6361 gatgcgggct tcggtcatcg gaccggtaag gttacgcaaa tcttcttttt ttgcctcaat
     6421 cagtcggtcg ataccataga gagccatacg gcggtagtcg ccgatgatac gtccgcgggc
     6481 atagttgtca ggaagtccgg tgaggaatcc cagagaacgg aacgaacgga tttcttcggt
     6541 atatacgtca aatactccgt cattgtgcgt cttgcggtag tgagtgaata tatcttttac
     6601 acggtcgtcc acttccacgc cgttttcgtg gcaggctttg ctcaccacgt tgatacctcc
     6661 gaaaggtttg attgcacgtt tcagaagttc gtctgtttgc agaccgacga tcagttcgtt
     6721 ttctttgtcg atatatccgg ctttatgaga agtgatggtt gagacggtga cattatccag
     6781 tgagcgtact ccgttattgg ctctttcttc tgccagtgct tcgaggcagc ggttccatac
     6841 agcttttgtg cgttcggtag gtccttcgag gaaagaagca tctccgtaat acggagtgat
     6901 gttatgactt acgaaatctc tgacattgat ttcagtgctc caaagaccgt ctttaaagat
     6961 cttatttaat tccataaatg ataaaattag aggttgtagt tatccttttc tttagagcgc
     7021 acaaagttac aacgtttttt catataatca gcataaatat gct
//
