LOCUS       c00215_gnlPro..         6906 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KAEIMFCC_215.
ACCESSION   c00215_gnlPro..
VERSION     c00215_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:30:16
            Original ID  :: gnl|Prokka|KAEIMFCC_215
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6906
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6906
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2697]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2697
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6906
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6906
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1955
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_1"
                     /sec_met_domain="Gly_radical (E-value: 4.1e-47, bitscore:
                     148.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1047.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QTDELLKRAIKPFGGINVVSKACHENGVEVDDRVKDIFTHYRKTH
                     NDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALYGIDRLIEAKKEDLHNL
                     TGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVK
                     EQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDI
                     FAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKE
                     FCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLL
                     AINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDK
                     YYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGE
                     FPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPD
                     GRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATDED
                     RIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSR
                     EHQLEVISRSFHERM"
     CDS             1969..2697
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2690..3139)
                     /locus_tag="ctg215_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3126..3293)
                     /locus_tag="ctg215_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(3353..3856)
                     /locus_tag="ctg215_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             4050..4280
                     /locus_tag="ctg215_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             4367..6481
                     /locus_tag="ctg215_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(6551..6856)
                     /locus_tag="ctg215_8"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
ORIGIN
        1 tgcagacaga cgaacttctg aaacgtgcca tcaagccttt cggaggtatc aacgtagtca
       61 gcaaagcctg tcacgaaaac ggcgtggaag tggacgaccg cgtaaaagat attttcactc
      121 actaccgcaa gacgcacaac gacggagtat tcgacgtata tacggaagaa atccgttcct
      181 tccgttcgct gggattcctt accggacttc ccgacaatta tgcacgcgga cgcatcatcg
      241 gtgactaccg ccgtatggca ctttacggca tcgaccgtct gatcgaagca aagaaggaag
      301 atttgcacaa cctcaccggt ccgatgacag aggcccgcat ccgcctgcgc gaagaagtgg
      361 cagaacagat caaggcactg aaagacatga aagtgatggg cgaatactac ggtctcgacc
      421 tgagccgtcc cgcttacacg gcacaagaag ccgtacagtg ggtatatatg gcttaccttg
      481 ccgccgtcaa agaacaagac ggtgccgcca tgtcactggg taacgtttct tctttcctcg
      541 atatctatct ggaatatgaa ctgagcaaag gaaccatcac cgaatcattc gcgcaagagc
      601 tgatcgacca gtttgtcatc aaactgcgta tggtccgcca cctgcgtatg caatcataca
      661 acgacatctt tgcaggcgac ccgacctggg taaccgaatc tctgggcgga cgtctcaacg
      721 acggacgtac caaagtgaca aagacctcct tccgtttcct gcagacattg tacaacctcg
      781 gcccttcacc ggaaccgaac ctgaccgtac tctggagccc ggaacttccg gaaggattca
      841 aagaattctg tgcaaaggtt tctatcgaca cttcttctat ccagtacgaa aatgacgacc
      901 tgatgcgcga agtacgtcag tccgacgact acggaatcgc ctgctgcgta tcataccagg
      961 aaataggtaa gcagattcag ttcttcggcg cccgttgcaa cctggccaaa gccctgttgc
     1021 ttgccatcaa cggcggacgt tgcgagaata ccggtacagt catggtgaag aacatccccg
     1081 tactgaccag tgacacgctg aagttcgaag aagtaatgga caactacaaa aaagtgctga
     1141 ccgaaatcgc ccgcgtatat aacgaagcga tgaacatcat ccactatatg cacgacaagt
     1201 attactacga gaaggcccaa atggctcttg tagatacgaa cccgcgcatc aacctggctt
     1261 acggtgtagc cggactttcc atcgccctcg attcactgtc tgccatcaaa tatgcgaaag
     1321 taactgcccg ccgcaatgat atcggtctga cagaaggctt cgacatcgaa ggagaattcc
     1381 cttgcttcgg taacgacaac gacaaggtag accaccttgg cgtagacctg gtatacttct
     1441 tcagcgaaga attgaagaaa ttgcctgttt acaagaatgc ccgtcctacc ctctccttgc
     1501 tgactatcac ttccaatgtg atgtacggca aaaagaccgg tgctactccc gacggacgtg
     1561 ccaaaggtgt tgccttcgct ccgggtgcca acccgatgca cggacgcgac aagaatggtg
     1621 cgatcgcttc tctgagttct gtagcgaaac ttcgttaccg tgactcacag gacggtatca
     1681 gcaacacctt ctctatcgtt ccgaaatcac tgggtgctac ggacgaagac cgtatcgaga
     1741 acctcgtaac gatgatggac ggttacttca ccaaaggtgc ccaccacctg aacgtaaacg
     1801 tactgaaccg tgatatgctg tacgatgcca tggagcatcc ggaaaactat ccgcagctga
     1861 ctatccgcgt ttccggctat gccgtaaact tcgtgaagct gagccgcgaa caccaactgg
     1921 aagttatcag ccgtagcttc cacgaacgta tgtaatctat tactctatat gatgataaac
     1981 gtacattcat acgaaagtat gggaacattc gacgggccgg gcttacggct cgtcgttttt
     2041 cttcaaggat gcaacttccg ctgcctctat tgcgccaatc cggatacgat agcaggaaag
     2101 ggaggcacgc ctaccccacc ggaagaaatc gtccgcatgg caatgagcca gcgccccttc
     2161 ttcgggaaac gaggaggaat cactttctcc ggaggcgaac cgacgtttca ggcaaaagca
     2221 ctcgtcccgc tggtccgcga actgaaagaa aaaggcatcc atgtctgtct ggacagcaac
     2281 ggaggacttt ggaacgaaga cgtagaagaa ttattcagac tgaccgatct tgtgttattg
     2341 gatataaagg aattcaaccc caaccgccat cagacgctca ccggaagaag caacgagcaa
     2401 accatccgca cggccgcctg gctggaagaa caggagaaac ctttctggct acgctacgtg
     2461 ctagtgcccg gatacagcga ttttgaagaa gacatacgcg cactgggaga agcactcgga
     2521 aaatacaaga tgatccaacg ggtggaaata ctgccttacc atacactggg cgtacataag
     2581 tacgaagcga tgggacagga atacaagatg aaaggggtaa aagaaaatac ccccgaacag
     2641 cttgaaaaag ctgcggaggt attcaaagag tatttcacta cggtggtagt taattaatgg
     2701 agaattctcc aatgacctca aagcaagggc attctttaat ccgttcccaa ggatctacga
     2761 tcccattata gttggtgtcg ggacttaaat cccgatgccc tactacttta gcttccggat
     2821 aacgctgcaa caattcgtgt accaagcgat gtagcgcctc cttctgacgg ggggtacgcg
     2881 tatccgcagc ttttccattc tcatccagac cgccctcata acatacacct aagctgatcg
     2941 cattatagcc acgtgcatga gctccgaccg tatcttcatc ccgcatcggc tcaatctcgc
     3001 ccgacttgcg tatataataa tgatatcccc agcacgaaaa gccacgataa cggtgagccg
     3061 tatccacatc ctttgccgta aagtcgcaat cgacacgagt ggcggaacaa tggatcacaa
     3121 tcagactaat ctttctcatg ttccttttcc ctttccgtcg ttttcacttt gcacacctcc
     3181 ttacaagtag tatgatagcg cacttctctc accaccctgt cccgctttct ctttccgaag
     3241 aaaggaatca gatacatgat cacatctaaa atccgactta ctatttcttt catcttatca
     3301 aaatattaat ggaagttatt ctaattcgtt attaccttga cccgtctgcc aattataccg
     3361 tagggtcggg agcctcttca tcaccggagc catcaccacc ggaatccgga ttcttgtcag
     3421 ggtcttctcc cccgccatcg cctgcacccg cctgcgccac cgcacgagtc tgcacttgct
     3481 gataactcag aatcttcatc aactccgcca gcgtcttacc ggggcggaaa ataagatgcg
     3541 cccccttaat cattgaaccg gaaaattcct tgaccgatgc cgcaccttca ctgttcagtc
     3601 ctatctgcaa actgcccaaa tcgcccagtc tcgcaattct tcctgccttc aacgcacgtt
     3661 tcacttcgta aagaataccc gaaatagccg cacgtacatc cgtttccgta caagtagaac
     3721 gactggtgat cgcttcgcac aactcttcaa agtccagctc acccgacgcc tgcgcctgtg
     3781 cataatactt tgccggttcg ctcttttttg caggattctt gcgcggcacc actgaataag
     3841 atactgtcat tgccataata aaataatttt aaagtttata atacatagga tgcatcccct
     3901 tcttgttaac agcacaaaga taaggatagc cggacaagcc atattcccca acgaagtttc
     3961 ttgattccca acgaataaaa acgaagtcaa acctttgaat aacaaggaat tttatgtaac
     4021 tttatagcat aaaacaagaa gaaaacatca tgaattacaa agaagattct ttccttatcc
     4081 gcacctatac caaagcggaa ctggctcacc tttacaatcc ccatgtctgc ctcaaagttg
     4141 ctttacaaat acttcgtcgt tggattattt acaacctccc tctcctgcac gaactcgaac
     4201 aggaaggcta ccgcgcacgc aaccgtctcc tttcgccgaa acaagtggca actatcatac
     4261 actttttggg cgaaccgtag taacttattg ccatttcata caaagttcct ttacacattt
     4321 ccgcaaagaa gttcctttgc atcgacaaac aataatcaaa ataaaaatga aacatttcac
     4381 catatttcaa ggtttctact acgcaatagc agagatgacc gaagaagaaa tagtgtccac
     4441 tatcggcagt tttacctacc gggaaaaagt cgaagaaata cgccgcatct ttgcagagca
     4501 aggagaaaag gctgccaatg agaagaaaaa agaattgccg gcaatcgcct tctccgccag
     4561 ctaccgagga aggcgtacca aagtgaacct cgtcaaatat ctgggacaca tcgtgataga
     4621 catcgaccac ctcagcaaag aagaattggc acgcatcctc cctatcatca agagatgcga
     4681 ttacacccgc atcgccttta tcagccccaa agggatgggc gtgaaaatca tagtacgcgc
     4741 atgccatccg gatgagacat tgcccgaaac ccttcaggaa atagaagact tccatcatgc
     4801 agcttacacc agactcgttt ctttttacac cgaactctgt cagatagaaa tcgacacctc
     4861 cggtcaggat gtggcacgca cttgtctgtt ctcttacgat ccggagattt atttcaatcc
     4921 gaacgctgac gcttttcttg tagatcaacc tcaggcatcc tacaaggttt caaatcgtaa
     4981 aaatctctcc ggcagtaaac aacagactcc tccggacggc cccccgacga atgaagacac
     5041 agcgctcaac gcccattccg ctaacgcttc tctggtgctg actttaacct actatcacaa
     5101 caagtccgaa aaatacatag caggcaaccg gaacaattat ctgcaccatc tctcatgtac
     5161 cttcaatcgc tacgggattc cgcaggagga agcatccgct tttatcaaaa gccagtttac
     5221 agactttccg gcggacgaga cagtctcttt gatcaatagc gcctacgctc ataccgacga
     5281 atttaatacc tgtaaactga atggcacgca gaaacgaata ttacggatag agcaatatat
     5341 cagcgagcac tacgaaacac gctacaacga agtgctgcat atcatggaat atcgcagaag
     5401 gcatcccgac accgagaaac cggagccttt ccgcatcttg gatgagatga tggaaaacag
     5461 tatctggata gaaatcaacg agctgggata tccctgcact gtcaaaacaa tcgaaaatct
     5521 gatttactcc gatttcagcc agtcgtatca tcccattcgc gagtactttg aattactgcc
     5581 cgaatgggac ggaaccgact atatccgtat cctggcggat agtgtccaga cgagtcatca
     5641 ggagttttgg gcggaatgcc tggaacgtta tctggtggcg atgtgcgcag cggctactca
     5701 ggagaatatt gtcaatcaca cggtgcttct gctatgcagc gagatacaga acataggaaa
     5761 gactaccttt atcaataatc tcctgcctcc cgaactgcgt gcttatctct ctaccggact
     5821 aatcaattcg aacaataaag atgatttagc caaaataacg caggccatgc tgatcaatct
     5881 cgacgagttt gaagggatga gcggccggga actgaatgca ttcaaagatt tggtaacgcg
     5941 caaagtgatc agtttccgtc tgccttatgc acgccgttcg caaaacttcc ctcatacggc
     6001 ctcttttgca ggaacctgca attatcagga agtgcttcac gacacgacag gcaaccggcg
     6061 tttcctttgt tttcatcctt attccatcca atttattacc atcaattatg cgcagctcta
     6121 tgctcagatc aaatatctgc tgaataagcc gggatatcaa tactggttca cgcaagcgga
     6181 caatgaacgg cttgaagaga ataacgaggc tttcatcttt cattcaccgg aagaagaaat
     6241 ggttcttacg catatccgca agccggaacg tttcgagaag gtttattatc tgactgtcag
     6301 tgagattgcg gaactaatac gtgagcgtac cggttatcag tattccatcg gatcaaaaat
     6361 acagttgggg aaggtgatga ctaagcacca ttttgaatct aaaaaaggaa ataacgggcg
     6421 ccgatatgcc gtatttatta tagatatcga gcaggtaaaa tcaaacaggc tgtatgaata
     6481 gaagcgggaa agccttgtga atatagcaaa atgcagagtc atcttgacag atactctgca
     6541 tttattgttt ttacaaaatc attgaaaatt cgattttaca gcctaacgca ttcattatac
     6601 gatagaaagt agatacttta ggctctgtct gaccactttc tactcttgaa atataagaac
     6661 ggtcagaacc gatacgccgg gctaattcag cctgagtgat tttagccttt tttcttgcgt
     6721 cctcgataat ctgtcctgta taaaaggcgt aggccttttc ttctgcctct gcacgagaag
     6781 ctgttccttc cttaccaaaa cgctcatcta ttaatgcact gacattaaag aattcctcat
     6841 ttgtttttgc ttccataata ctcctttctt aatatctttg ctttatcaat ttctttcttt
     6901 ggtgtt
//
