LOCUS       c00178_gnlPro..         7102 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ENFLOKOM_178.
ACCESSION   c00178_gnlPro..
VERSION     c00178_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 16:36:50
            Original ID  :: gnl|Prokka|ENFLOKOM_178
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7102
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7102
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[124:3097]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      125..3097
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7102
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7102
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             125..2353
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg178_1"
                     /sec_met_domain="Gly_radical (E-value: 3.4e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2369..3097
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg178_2"
                     /sec_met_domain="Radical_SAM (E-value: 3e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3125..3568)
                     /locus_tag="ctg178_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3555..3722)
                     /locus_tag="ctg178_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(3743..4246)
                     /locus_tag="ctg178_5"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             4440..4670
                     /locus_tag="ctg178_6"
                     /transl_table=11
                     /translation="MYYPDQPFHICTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             4758..6875
                     /locus_tag="ctg178_7"
                     /transl_table=11
                     /translation="MDHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPIFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCATATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             6990..7100
                     /locus_tag="ctg178_8"
                     /transl_table=11
                     /translation="MLEIYPHLPSPFPFSSSKKRGCVKTLTQPFFYAQSPD"
ORIGIN
        1 atatctaggt attttatgtt gaaaagcata aaatagagca tatttatgct gattatatga
       61 aaaaacgttg taactttgtg cgctctaaag aaaaggataa ctacaacctc taattttatc
      121 atttatggaa ttaaataaga tctttaaaga cggtctttgg agcacagaaa tcaacgtaag
      181 agatttcgtt agtcacaaca tcactccgta ttatggagat gcttctttcc tcgaaggacc
      241 taccgaacgc actaaagctg tatggaaccg ctgcctcgaa gcactggcag aagaaagagc
      301 caataacgga gtacgctcac tggataatgt caccgtctca accatcactt ctcataaagc
      361 cggatatatc gacaaagaaa acgaactgat cgtcggtctg caaacagacg aacttctgaa
      421 acgtgcaatc aaacctttcg gaggtatcaa cgtggtgagc aaagcctgcc acgaaaacgg
      481 cgtggaagtg gacgaccgtg taaaagatat attcactcac taccgcaaga cgcacaatga
      541 cggagtattt gacgtatata ccgaagaaat ccgttcgttc cgttctctgg gattcctcac
      601 cggacttcct gacaactatg cccgcggacg tatcatcggc gactaccgcc gtatggctct
      661 ctatggtatc gaccgactga ttgaggcaaa aaaagaagat ttgcgtaacc ttaccggtcc
      721 gatgaccgaa gcccgcatcc gcctgcgcga agaagtggca gagcaaatca aggcattgaa
      781 ggacatgaag gtaatgggcg aatactacgg actcgacttg agccgtcctg cctacaccgc
      841 acaagaagca gtacaatggg tgtacatggc ttaccttgct gccgtaaaag aacaagacgg
      901 tgctgccatg tcactgggta acgtctcttc gttcctcgac atttatatgg aatatgaatt
      961 gagcaaagga actatcacag aatctttcgc acaggagctg atcgaccagt ttgtcatcaa
     1021 actgcgtatg gtgcgccacc tgcgtatgca atcttacaac gacatctttg ccggcgaccc
     1081 gacatgggta accgaatccc tgggcggacg cctcaacgac ggacgcacca aggtgacaaa
     1141 gacttctttc cgcttcctgc aaacactcta caacctcggc ccttcaccgg aaccgaacct
     1201 gactgtattg tggagcccgg aactgccgga aggattcaaa gaattctgcg caaaagtatc
     1261 tatcgacact tcttccatcc aatatgaaaa cgacgacctg atgcgtgaag tgcgccagtc
     1321 tgatgactac ggaatcgcgt gctgcgtatc ttatcaggaa atcggaaaac aaatccagtt
     1381 cttcggcgca cgttgcaacc tggctaaagc cctgctgctt gccatcaacg gcggacgttg
     1441 cgaaaacacc ggtacggtaa tggtgaaaaa catccccgta ctaaccagcg acacactgaa
     1501 cttcgaagaa gtaatgagca actacaagaa ggtattgaca gaaatcgccc gtgtttacaa
     1561 cgaagcgatg aacatcatcc attatatgca tgataagtat tactacgaaa aagcccaaat
     1621 ggctcttgta gatacaaacc cacgcatcaa ccttgcttac ggtgtagccg gactttctat
     1681 cgccctcgat tcactgtcgg ccatcaaata tgccaaagta accgcacgcc gcaacgacat
     1741 cggtctgaca gaaggtttcg atattcaagg cgaattccct tgcttcggta atgacaacga
     1801 caaggtagac cacctgggtg ttgacctggt ctatttcttc agcgaagaat tgaagaaact
     1861 tccggtttac aagaatgccc gtcctacttt atctttgctc accatcactt ccaacgtgat
     1921 gtatggcaag aaaacaggtg ctactcccga cggacgcgcc aaaggtgtcg cttttgctcc
     1981 gggagccaac ccgatgcacg gacgtgacaa gaacggtgct atcgcctctt tgagttccgt
     2041 agcgaaatta cgttaccgcg actcacaaga cggtatcagc aacaccttct ccatcgttcc
     2101 gaaatcactg ggagctactg aagaggatcg cgtagaaaat ctggtgacaa tgatggatgg
     2161 ttacttcacc aaaggcgctc accacctgaa cgtgaacgta ctgaaccgtg aaatgcttta
     2221 cgatgccatg gaacatccgg aaaaatatcc gcaactcacc atccgtgttt ccggttatgc
     2281 cgtaaacttc gtgaagttga gccgcgaaca tcaattggaa gttatcagcc gtagcttcca
     2341 cgaacgtatg taatcagatc tactcaatat gacgataaac gtacattcat acgaaagtat
     2401 gggaacattc gacgggccgg gcttacggct cgtcgttttt cttcaaggat gcaatttccg
     2461 ctgcctgtat tgtgccaacc ccgacacgat agccggaaaa ggaggcacac ctaccccacc
     2521 ggaagaaatc gtccgcatgg ctatgagcca acgtcctttt ttcgggaaac gcggaggagt
     2581 cactttctcc ggaggagaac ctacgtttca ggccaaagca ctagtccctt tagtgcgtga
     2641 actgaaagaa aagggaatac atgtatgcat agacagcaac ggcggtatct ggaacgaaga
     2701 agtagaagaa cttttcaaac tgacggatct tgtattgctg gatattaaag agttcaatcc
     2761 ggcacgccat caggcactga ccggaagaag taacgagcaa accatccgca cggcagcatg
     2821 gttggaagaa aacgaaaaac ctttctggct gcgttatgta ttagtgcccg gatacagcga
     2881 tttcgaggaa gacattcgcc gactgggaga ggctctggga aaatataaga tggtgcaacg
     2941 agtggaaatc ctgccttatc atacattagg cgtacataag tatgaagcaa tggagcagga
     3001 atacaagctg aaagacgtaa aagaaaatac ccccgaacaa cttgaaaaag ccgcggaggt
     3061 atttaaagag tatttcacta ctgtggtggt caattaacca ccacagtaat aaataggata
     3121 aaaatcacca tgtctctgct ttcacctcaa aacagggaca ttctttgatc cgttcccagg
     3181 ggtctacaat tccgttgtag ttggtgtcag ggctcaagtc ccggtgaccg accacctttg
     3241 catccggata acgctgcaac aactcgctta ccagacgatg catcgcctct ttctggcggg
     3301 aagtacgtgt atccgctgcc tttccgttct catccagccc gccttcatag cacaccccta
     3361 aactaatcgc attaaaaccg cgtgcatgag cacccaccgt atcttcatcc ctcatcggtt
     3421 ctatctgtcc cgacttgcgg atataataat gataccccca acacgaaaag ccccgaaagc
     3481 gatgggcagt gtccacatcc ttcgccgtga agtcgcgatc gacacgggta gcagagcagt
     3541 gaatcacaat cagactaatc tttctcatcg tccttctccc tttccgtcgt tttcacatta
     3601 cacacttcct tatgggtagc attgaagcgc acctctctca caattctgtt ccgctttctt
     3661 tttccgaaga aaggcacaag gaacatgatt acatccaaaa tcttagttac aatttctttc
     3721 atttgatcta atattaaagt tatcatgctg ccggatccgg agcttctccg cttccaccac
     3781 cgtctgaacc accaccggaa cctttgtcat cgtcttctcc ttcattgccg ctaccgcccg
     3841 tttgagccac cgcacgcgtc gttacctgct ggtagctaag aatcttcgtg atgtccgcca
     3901 acgtctttcc gggacggaaa ataatatggg cagccgtaat cagcgaactt gaaaactcct
     3961 tgaccgacac agctccttca ctgttcaacc cgatctgcaa actgcccaaa tcacccagcc
     4021 ggactattct tcccgctttc aacgcacgct tcgcctcata aagaataccc gaaatggcgg
     4081 cacgcacatc tgtctccgta caggtagacc gactcgtgat tccctcgcac aactcttcaa
     4141 aatccaactc tcccgaagcc tgtgcctgcg cgtaatactt tgccgactca tcctttttgg
     4201 caggattctt gcgcggcact actgaataag ttactgtcat tgccataata aaataatttt
     4261 aagatttcac acatttcgaa cgcgttcctt atttgttaac gacataaaga taacacattt
     4321 ttcaccctcc ggattcccca acgaagtttc ctgatactct atgaataata atgacgttat
     4381 tcttttatta aaccattaat tttcagtaac tttatagcat aaaccaaata tcattcatca
     4441 tgtattatcc ggatcagccg tttcatatct gcacttataa aaagtcggaa ctagctcatc
     4501 tttacaaccc caacgtatgc cttaaagtag ccttacaaat actacgtcgt tggattgtct
     4561 acaaccttcc gctcctgcaa gaactcgaac aagaaggcta tcgtgcccgc aaccgccttc
     4621 tctctccccg gcaagtggca acgatcatac gctatttggg agaaccctag gaacttaatc
     4681 aaaaaaacga aaagttccat tctattattt tacaaacaaa tttctttgca accgcaaatt
     4741 aaaaagaaca aaccaacatg gatcatttta ccatctttca agacttttac cgcgtcatag
     4801 ccgaaatgac agaagaagaa atcgtatcgg ccatcagcaa cggcacatac cgggaaacag
     4861 tggaggacgt gcgccgaatc ttcgcggaac aaggcgagaa agcagccaac gaaaagaaaa
     4921 tagaactacc ggaaatcacc ttttcagcca actatcgggg aggacgttca aacgcaactc
     4981 tcgtcaagta tctgggatac atcgtggtag acatcgacca tcagacacag gaagcattag
     5041 cacgcatcct cgcactggca aaaaagtgtg cccacacccg catcgctttc atcagtccca
     5101 aaggaatggg actgaaaatc gtggtacgtg tatgccggac ggacgggaca ttgcccgaaa
     5161 cgattcagga aattgaagat ttccatcatg ccgcttatca caaaatcgct tccttttatg
     5221 cccagctctg cggagttgag gtcgacactt ccggacaaga tgtcagccgt acttgcctgt
     5281 tctcttacga tccggatatt tatttcaatc ccgatgccac agctgtcatc gtggaacagc
     5341 caccgatatt cttcaagtct caaaagaaga aaagggcatc cggcaaaaag aaaaagacga
     5401 cagcaccaga caacaatccc ctcaccgaac aagtggcact caactaccat tcctcgcacg
     5461 cttccttaat ggtgactctg aactattatc acaataaatc cgaaaagtac gtcaccggca
     5521 accggaacaa ctacctgcat tgcctggctt gtatgtacaa tcgctacggc gtcccccagg
     5581 aagaggcggc agcttttatc aagagccagt tcaccgatct tcccgcggat gaaatggacg
     5641 cactgatcgg gagtgcttac ggacacaatg aggaattcga cacccgcaag ctgaatagta
     5701 cgcagaagcg aatattacag attgagcaac atatcaaaga aaattacgat acacgctaca
     5761 acgaagtgct tcatatcatg gaatatcgca gaaggaaaac ggacacggag caaccggaac
     5821 ctttccatat tctcgacgaa atgatggaaa acagcatttg gatggagatg aacgaactag
     5881 gctattcgtg caccgtcaaa acgattcaga atctgatcta ctccgacttc agcagcacct
     5941 accatcctat tcgcgagtat ttagactcgc tgcctgaatg ggacggaacg gattatatcg
     6001 gtatccttgc caacagcgtg catactagtc accagaaatt ttgggtagaa tgtctggaac
     6061 gttatctggt gggaatgtgc gctaccgcca cacaagatga tgtggtgaac cacactgttt
     6121 tgttgctttg cagcgaaata cagaacattg gaaagactac gtttatcaac aacctgctgc
     6181 cgcccgaatt gcgcgcctat ctttctaccg gtcttatcaa cccgaacaac aaggatgatc
     6241 tggccaaaat agcgcaggcg atgcttatca atctggatga gtttgaaggg atgaacggac
     6301 gggaactgaa tatcttcaag gatttagtta cccgcaaagt aatcagcatc cgcctgccat
     6361 acgcgcggcg ttcgcagaac ttccctcaca cggcttcttt tgccggcaca tgcaattatc
     6421 aggaagtgct tcacgacacc acaggtaacc gccgttttct ctgctttcat gtcaactcga
     6481 tggagtttat caaaataaac tacgcgcagt tgtatgcaca aatcaagtat ctactcaaca
     6541 agccggggta tcagtattgg ttcacccagt cggaaaactc acggattgaa gagaacaacg
     6601 aggatttcat tttccactca ccggaagaag aactggtgtt gacacatatc cgcaaaccgg
     6661 aacgtttcga aaaggtgcat tatctgacgg tcactgaaat tgcggaattg attcgcgaac
     6721 ggacggggta tcaatattct cacggaacca aagcccaact aggaaaagtg atgtccaaac
     6781 acggcttcga atttcataag ggaaaaaacg ggagaagata cactgtattc attattgata
     6841 cggaacaggt gaaatcaaac aggctatatg aataagattt catctccttc gcacgcaaaa
     6901 aggtgagggt tggtgagggt acttttgcaa cttttcattt ttatatttct tatttctatt
     6961 cttctatttt cttctaaaaa aaaatacgaa tgttagaaat ttaccctcac ctaccctcac
     7021 cttttccttt ttcttcctca aaaaaacgag gttgtgtcaa aacgctgaca cagccttttt
     7081 tttatgcgca aagcccagac tt
//
