GCA_958452905.1 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaFKIMNIKK_13 (original name was: gnl|Prokka|FKIMNIKK_13)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	cyclic-lactone-autoinducer	18,747	39,480

gnlProkkaFKIMNIKK_14 (original name was: gnl|Prokka|FKIMNIKK_14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	NRPS	19,106	68,090	dipeptide aldehydes	NRP	58%

gnlProkkaFKIMNIKK_17 (original name was: gnl|Prokka|FKIMNIKK_17)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	ranthipeptide	1	12,690

gnlProkkaFKIMNIKK_18 (original name was: gnl|Prokka|FKIMNIKK_18)

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	lanthipeptide-class-ii	33,933	56,593

gnlProkkaFKIMNIKK_22 (original name was: gnl|Prokka|FKIMNIKK_22)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	ranthipeptide	47,092	68,680

gnlProkkaFKIMNIKK_26 (original name was: gnl|Prokka|FKIMNIKK_26)

Region	Type	From	To	Most similar known cluster		Similarity
Region 26.1	RRE-containing	1	11,504

gnlProkkaFKIMNIKK_32 (original name was: gnl|Prokka|FKIMNIKK_32)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	cyclic-lactone-autoinducer,RRE-containing	13,254	46,260

Region	Type	From	To	Most similar known cluster	Similarity
Region 13.1	cyclic-lactone-autoinducer	18,747	39,480
Region 14.1	NRPS	19,106	68,090	dipeptide aldehydes	NRP	58%
Region 17.1	ranthipeptide	1	12,690
Region 18.1	lanthipeptide-class-ii	33,933	56,593
Region 22.1	ranthipeptide	47,092	68,680
Region 26.1	RRE-containing	1	11,504
Region 32.1	cyclic-lactone-autoinducer,RRE-containing	13,254	46,260

Compact view

gnlProkkaFKIMNIKK_13 - Region 1 - cyclic-lactone-autoinducer

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Location: 18,747 - 39,480 nt. (total: 20,734 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaFKIMNIKK_14 - Region 1 - NRPS

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Location: 19,106 - 68,090 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 19105 - 68090:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg14_34

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaFKIMNIKK_17 - Region 1 - ranthipeptide

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Location: 1 - 12,690 nt. (total: 12,690 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaFKIMNIKK_18 - Region 1 - lanthipeptide-class-ii

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Location: 33,933 - 56,593 nt. (total: 22,661 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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Lanthipeptides

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Lanthipeptide predictions

Lanthipeptide(s) for ctg18_35

ctg18_34

Leader:

MKNNEKNFIGDINKELEEIEKLDILEEIEESLLTMLGS

Core:

VNSLFCCH

Core with dehydrations:

VNDhaLFCysCysH

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg18_34 - Class II: Cleavage pHMM score: -3.20; RODEO score: 0; Monoisotopic mass: 903.4 Da; Molecular weight: 904.1 Da; Number of bridges: 1

gnlProkkaFKIMNIKK_22 - Region 1 - ranthipeptide

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Location: 47,092 - 68,680 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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gnlProkkaFKIMNIKK_26 - Region 1 - RRE-containing

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Location: 1 - 11,504 nt. (total: 11,504 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

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regulatory genes

other genes

resistance

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Gene details

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaFKIMNIKK_32 - Region 1 - RRE-containing,cyclic-lactone-autoinducer

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Location: 13,254 - 46,260 nt. (total: 33,007 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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No matches found.