LOCUS       c00267_gnlPro..         7727 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CAKCHGEE_267.
ACCESSION   c00267_gnlPro..
VERSION     c00267_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-08 00:20:49
            Original ID  :: gnl|Prokka|CAKCHGEE_267
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7727
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7727
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4470:7443]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4471..7443
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7727
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7727
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..80
                     /locus_tag="ctg267_1"
                     /transl_table=11
                     /translation="FTVKVVYIRFIGTHKEYDKIDCANI"
     CDS             85..450
                     /locus_tag="ctg267_2"
                     /transl_table=11
                     /translation="MTKIENQTQYEWAVKRVEDLLPLVKDDTPLNDPNSIELELLSNFV
                     ADYSEEHFSLGEPSLVDVLKLRMYEMGLNQKSLSQLIGVSPSRLSDYISGKCEPTLKVA
                     REISKKLNIDASIVLGV"
     CDS             473..604
                     /locus_tag="ctg267_3"
                     /transl_table=11
                     /translation="MVGNLKKITVFFKFPTVFLRKKKEKVRVGEGKFLTFVFFFRRK"
     CDS             complement(693..2810)
                     /locus_tag="ctg267_4"
                     /transl_table=11
                     /translation="MEHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPIFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSSTYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVDSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
     CDS             complement(2898..3128)
                     /locus_tag="ctg267_5"
                     /transl_table=11
                     /translation="MYYPDQPFHICTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             3322..3825
                     /locus_tag="ctg267_6"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             3822..4013
                     /locus_tag="ctg267_7"
                     /transl_table=11
                     /translation="MITLILDQMKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATH
                     KEVCNVKTTEREKDDEKD"
     CDS             4000..4443
                     /locus_tag="ctg267_8"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(4471..5199)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg267_9"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5215..7443)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg267_10"
                     /sec_met_domain="Gly_radical (E-value: 4.3e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 agttcactgt aaaggttgta tatattcgct ttattggtac tcataaagaa tatgataaaa
       61 tagattgtgc taatatttga aaatatgaca aagatagaaa accaaaccca gtatgaatgg
      121 gctgtaaaga gagtggagga tcttcttccg ttagtgaagg atgatactcc tttgaatgac
      181 cctaacagca tcgagttgga acttctttct aattttgttg ccgattattc agaggaacac
      241 ttttctttgg gagaaccttc gcttgtggat gttctgaagc ttcgtatgta cgaaatgggg
      301 ctcaatcaga aatcactatc acaactgata ggagttagtc cttcgcgtct tagtgactac
      361 atttccggaa aatgtgagcc gacattgaaa gtggctcgtg aaataagtaa aaaactgaat
      421 attgatgcta gtattgtgct aggagtataa cttcgattca gattaaaaaa acatggtggg
      481 aaatttgaaa aagataactg tctttttcaa atttcccacc gtttttttga ggaagaaaaa
      541 ggaaaaggtg agggtaggtg agggtaaatt tctaacattc gtattttttt ttagaagaaa
      601 atagaagaat agaaataaga aatataaaaa tgaaaagttg caaaagtacc ctcaccaacc
      661 ctcacctttt tgcgtgcgaa ggagatgaaa tcttattcat atagcctgtt tgatttcact
      721 tgttccgtat caataatgaa tacagtgtat cttctcccgt tttttccctt atgaaattcg
      781 aagccgtgtt tggacatcac ttttcctagt tgggctttgg ttccgtgaga atattgatac
      841 cccgtccgtt cgcgaatcaa ttccgcaatt tcagtgaccg tcagataatg caccttttcg
      901 aaacgttccg gtttgcggat atgtgtcaac accagttctt cttccggtga gtggaaaatg
      961 aaatcctcgt tgttctcttc aatccgtgag ttttccgact gggtgaacca atactgatac
     1021 cccggcttgt tgagcagata cttgatttgt gcatacaact gcgcgtagtt tattttgata
     1081 aactccatcg agtcgacatg aaagcagaga aaacggcggt tacctgtggt gtcgtgaagc
     1141 acttcctggt aattgcatgt gccggcaaaa gaagccgtgt gagggaagtt ctgtgaacgc
     1201 cgcgcgtatg gcaggcggat gctgattact ttgcgggtaa ccaaatcctt gaagatattc
     1261 agttcccgtc cgttcatccc ttcaaactca tccagattga taagcattgc ctgcgctatt
     1321 ttggccagat catccttgtt gttcgggtta ataagaccgg tagaaagata ggcgcgcaat
     1381 tcgggcggca gcaggttgtt gataaacgta gtctttccga tgttctgtat ttcgctgcaa
     1441 agcaacaaaa cagtgtggtt caccacatcg tcttgcgtgg cggcagcgca cattcccacc
     1501 agataacgtt ccagacattc tacccaaaat ttctggtgac tagtatgcac gctgttggca
     1561 aggataccga tataatccgt tccgtcccat tcaggcagcg agtctaaata ctcgcgaata
     1621 ggatggtagg tgctgctgaa gtcggagtag atcagattct gaatcgtctt gacggtgcac
     1681 gaatagccca gttcgttcat ctccatccaa atgctgtttt ccatcatttc gtcgagaata
     1741 tggaaaggtt ccggttgctc cgtgtccgtt ttccttctgc gatattccat gatatgaagc
     1801 acttcgttgt agcgtgtatc gtaattttct ttgatatgtt gctcaatctg taatattcgc
     1861 ttctgcgtac tattcagctt gcgggtgtcg aattcctcat tgtgtccgta agcactcccg
     1921 atcagtgcgt ccatttcatc cgcgggaaga tcggtgaact ggctcttgat aaaagctgcc
     1981 gcctcttcct gggggacgcc gtagcgattg tacatacaag ccaggcaatg caggtagttg
     2041 ttccggttgc cggtgacgta cttttcggat ttattgtgat aatagttcag agtcaccatt
     2101 aaggaagcgt gcgaggaatg gtagttgagt gccacttgtt cggtgagggg attgttgtct
     2161 ggtgctgtcg tctttttctt tttgccggat gcccttttct tcttttgaga cttgaagaat
     2221 atcggtggct gttccacgat gacagctgtg gcatcgggat tgaaataaat atccggatcg
     2281 taagagaaca ggcaagtacg gctgacatct tgtccggaag tgtcgacctc aactccgcag
     2341 agctgggcat aaaaggaagc gattttgtga taagcggcat gatggaaatc ttcaatttcc
     2401 tgaatcgttt cgggcaatgt cccgtccgtc cggcatacac gtaccacgat tttcagtccc
     2461 attcctttgg gactgatgaa agcgatgcgg gtgtgggcac acttttttgc cagtgcgagg
     2521 atgcgtgcca atgcttcctg tgtctgatgg tcgatgtcta ccacgatgta tcccagatac
     2581 ttgacgagag ttgcgtttga acgtcctccc cggtagttgg ctgaaaaggt gatttccggt
     2641 agttctattt tcttttcgtt tgctgctttc tcgccttgtt ccgcgaagat tcggcgcacg
     2701 tcctccactg tttcccggta tgtgccgttg ctgatggccg atacgatttc ttcttctgtc
     2761 atttcggcta tgacgcggta aaagtcttga aagatggtaa aatgctccat gttggtttgt
     2821 tctttttaat ttgcggttgc aaagaaattt gtttgtaaaa taatagaatg gaacttttcg
     2881 tttttttgat taagttccta gggttctccc aaatagcgta tgatcgttgc cacttgccgg
     2941 ggagagagaa ggcggttgcg ggcacgatag ccttcttgtt cgagttcttg caggagcgga
     3001 aggttgtaga caatccaacg acgtagtatt tgtaaggcta ctttaaggca tacgttgggg
     3061 ttgtaaagat gagctagttc cgacttttta taagtgcaga tatgaaacgg ctgatccgga
     3121 taatacatga tgaatgatat ttggtttatg ctataaagtt actgaaaatt aatggtttaa
     3181 taaaagaata acgtcattat tattcataga gtatcaggaa acttcgttgg ggaatccgga
     3241 gggtgaaaaa tgtgttatct ttatgtcgtt aacaaataag gaacgcgttc gaaatgtgtg
     3301 aaatcttaaa attattttat tatggcaatg acagtaactt attcagtagt gccgcgcaag
     3361 aatcctgcca aaaaggatga gtcggcaaag tattacgcgc aggcacaggc ttcgggagag
     3421 ttggattttg aagagttgtg cgagggaatc acgagtcggt ctacctgtac ggagacagat
     3481 gtgcgtgccg ccatttcggg tattctttat gaggcgaagc gtgcgttgaa agcgggaaga
     3541 atagtccggc tgggtgattt gggcagtttg cagatcgggt tgaacagtga aggagctgtg
     3601 tcggtcaagg agttttcaag ttcgctgatt acggctgccc atattatttt ccgtcccgga
     3661 aagacgttgg cggacatcac gaagattctt agctaccagc aggtaacgac gcgtgcggtg
     3721 gctcaaacgg gcggtagcgg caatgaagga gaagacgatg acaaaggttc cggtggtggt
     3781 tcagacggtg gtggaagcgg agaagctccg gatccggcag catgataact ttaatattgg
     3841 atcaaatgaa agaaattgta actaagattt tggatgtaat catgttcctt gtgcctttct
     3901 tcggaaaaag aaagcggaac agaattgtga gagaggtgcg cttcaatgct acccataagg
     3961 aagtgtgtaa tgtgaaaacg acggaaaggg agaaggacga tgagaaagat tagtctgatt
     4021 gtgattcact gctctgctac ccgtgtcgat cgcgacttca cggcgaagga tgtggacact
     4081 gcccatcgct ttcggggctt ttcgtgttgg gggtatcatt attatatccg caagtcggga
     4141 cagatagaac cgatgaggga tgaagatacg gtgggtgctc atgcacgcgg ttttaatgcg
     4201 attagtttag gggtgtgcta tgaaggcggg ctggatgaga acggaaaggc agcggataca
     4261 cgtacttccc gccagaaaga ggcgatgcat cgtctggtaa gcgagttgtt gcagcgttat
     4321 ccggatgcaa aggtggtcgg tcaccgggac ttgagccctg acaccaacta caacggaatt
     4381 gtagacccct gggaacggat caaagaatgt ccctgttttg aggtgaaagc agagacatgg
     4441 tgatttttat cctatttatt actgtggtgg ttaattgacc accacagtag tgaaatactc
     4501 tttaaatacc tccgcggctt tttcaagttg ttcgggggta ttttctttta cgtctttcag
     4561 cttgtattcc tgctccatcg cttcatactt atgtacgcct aatgtatgat aaggcaggat
     4621 ttccactcgt tgcaccatct tatattttcc cagagcctct cccaagcggc ggatatcctc
     4681 ttcgaaatcg ctgtatccgg gcactaatac ataacgcagc cagaaaggtt tctcgttttc
     4741 ttccagccat gctgccgtgc ggatggtttg ctcgttactt cttccggtca gtgcttgatg
     4801 gcgtgccgga ttgaactctt taatatccag caatacaaga tccgtcagtt tgaaaagttc
     4861 ttctacttct tcgttccaga taccgccgtt gctgtctatg catacatgta ttcccttttc
     4921 tttcagttca cgcactaaag ggactagtgc tttggcctga aacgtaggtt ctcctccgga
     4981 gaaagtgact cctccgcgtt tcccgaaaaa aggacgttgg ctcatagcca tgcggacgat
     5041 ttcttccggt ggggtaggtg tgcctccttt tccggctatc gtgtcggggt tggcacaata
     5101 caggcagcgg aaattgcatc cttgaagaaa aacgacgagc cgtaagcccg gcccgtcgaa
     5161 tgttcccata ctttcgtatg aatgtacgtt tatcgtcata ttgagtagat ctgattacat
     5221 acgttcgtgg aagctacggc tgataacttc caattgatgt tcgcggctca acttcacgaa
     5281 gtttacggca taaccggaaa cacggatggt gagttgcgga tatttttccg gatgttccat
     5341 ggcgtcgtaa agcatttcac ggttcagtac gttcacgttc aggtggtgag cgcctttggt
     5401 gaagtaacca tccatcattg tcaccagatt ttctacgcga tcttcttcag tagctcccag
     5461 tgatttcgga acgatggaga aggtgttgct gataccgtcc tgtgagtcgc ggtaacgtaa
     5521 tttcgctacg gaactcaaag aggcgatggc accgttcttg tcacgtccgt gcatagggtt
     5581 ggctcccgga gcaaaagcga cacctttggc gcggccgtcg ggagtagcac ctgtcttctt
     5641 gccatacatc acgttggaag tgatggtgag caaagacaga gtaggacggg catttttgta
     5701 aaccggaagt ttcttcaatt cttcgctgaa gaaatagacc aggtcaacac ccaggtggtc
     5761 taccttgtcg ttgtcattac cgaagcaagg gaattcgcct tgaatatcga aaccttctgt
     5821 cagaccgatg tcgttgcggc gtgcggttac tttggcatat ttgatggccg acagtgaatc
     5881 gagggcgata gaaagtccgg ctacaccgta agcaaggttg atgcgtgggt tggtatctac
     5941 aagagccatt tgggcttttt cgtagtaata cttatcatgc atataatgga tgatgttcat
     6001 cgcttcgttg taaacacggg cgatttctgt caataccttc ttgtagttgc tcattacttc
     6061 ttcgaagttc agtgtgtcgc tggttagtac ggggatgttt ttcaccatta ccgtaccggt
     6121 gttttcgcaa cgtccgccgt tgatggcaag cagcagggct ttagccaggt tgcaacgtgc
     6181 gccgaagaac tggatttgtt ttccgatttc ctgataagat acgcagcacg cgattccgta
     6241 gtcatcagac tggcgcactt cacgcatcag gtcgtcgttt tcatattgga tggaagaagt
     6301 gtcgatagat acttttgcgc agaattcttt gaatccttcc ggcagttccg ggctccacaa
     6361 tacagtcagg ttcggttccg gtgaagggcc gaggttgtag agtgtttgca ggaagcggaa
     6421 agaagtcttt gtcaccttgg tgcgtccgtc gttgaggcgt ccgcccaggg attcggttac
     6481 ccatgtcggg tcgccggcaa agatgtcgtt gtaagattgc atacgcaggt ggcgcaccat
     6541 acgcagtttg atgacaaact ggtcgatcag ctcctgtgcg aaagattctg tgatagttcc
     6601 tttgctcaat tcatattcca tataaatgtc gaggaacgaa gatacgttac ccagtgacat
     6661 ggcagcaccg tcttgttctt ttacggcagc aaggtaagcc atgtacaccc attgtactgc
     6721 ttcttgtgcg gtgtaggcag gacggctcaa gtcgagtccg tagtattcgc ccattacctt
     6781 catgtccttc aatgccttga tttgctctgc cacttcttcg cgcaggcgga tgcgggcttc
     6841 ggtcatcgga ccggtaaggt tacgcaaatc ttcttttttt gcctcaatca gtcggtcgat
     6901 accatagaga gccatacggc ggtagtcgcc gatgatacgt ccgcgggcat agttgtcagg
     6961 aagtccggtg aggaatccca gagaacggaa cgaacggatt tcttcggtat atacgtcaaa
     7021 tactccgtca ttgtgcgtct tgcggtagtg agtgaatata tcttttacac ggtcgtccac
     7081 ttccacgccg ttttcgtggc aggctttgct caccacgttg atacctccga aaggtttgat
     7141 tgcacgtttc agaagttcgt ctgtttgcag accgacgatc agttcgtttt ctttgtcgat
     7201 atatccggct ttatgagaag tgatggttga gacggtgaca ttatccagtg agcgtactcc
     7261 gttattggct ctttcttctg ccagtgcttc gaggcagcgg ttccatacag ctttagtgcg
     7321 ttcggtaggt ccttcgagga aagaagcatc tccataatac ggagtgatgt tgtgactaac
     7381 gaaatctctt acgttgattt ctgtgctcca aagaccgtct ttaaagatct tatttaattc
     7441 cataaatgat aaaattagag gttgtagtta tccttttctt tagagcgcac aaagttacaa
     7501 cgttttttca tataatcagc ataaatatgc tgtattttat gcttttcaac ataaaatacc
     7561 tagatatagc tattctttgg aaatgatgtt cgtgattttt ttttgtgaaa actcttgcaa
     7621 gtttaaaata tattcgtacc tttgcaccgc ttttaacaaa aagcacttct gaaaaggaag
     7681 ttttggagag gtggcagagt ggtcgattgc ggcggtcttg aaaaccg
//
