LOCUS       c00489_gnlPro..         2832 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ALHLMMPL_489.
ACCESSION   c00489_gnlPro..
VERSION     c00489_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-08 00:31:00
            Original ID  :: gnl|Prokka|ALHLMMPL_489
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2832
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..765
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg489_1"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-47, bitscore:
                     148.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-45, bitscore:
                     144.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRV
                     IRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILT
                     ITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGIS
                     YTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQ
                     LTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     protocluster    1..2832
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1500]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1500
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2832
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2832
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             766..1500
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg489_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             1699..2277
                     /locus_tag="ctg489_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             2385..2831
                     /locus_tag="ctg489_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNII"
ORIGIN
        1 gataaatacg catatgaggc attcgagatg tcgcttcacg atggagatgt agagcgtatc
       61 cgtgcgacag gtatcgccgg tctttctatc gttgccgatt cgttggctgc tatccgtgat
      121 acgaaagtac gtgtgatccg tgacgagcgt ggcttggcgg ttgatttcga acgtgagggc
      181 gaatatgttc ctttcggtaa taacgacgat cgtacggata gtatcgctgt agagatcacc
      241 gagaagttca tggaatattt gcgtcagcac gagacctatc gccatgcgaa acctacgcag
      301 tccatcctta cgatcacctc taatgtggta tatggtaaga agactggtac gacaccggat
      361 ggccgtatcg gtggaactcc tttcgctccg ggcgcgaacc ctatgaacgg tcgtgatacg
      421 aaaggtgcta tcgctgcgct agcttcggta gcgaagcttc cattccagca tgcgcacgac
      481 ggtatctctt atactttcgc tgtttctccg gctacgttgg gaaaggagcg tgaagtgcag
      541 atcaataatc tggttagctt attggatggc tattttactc cggatggagg tcagcatctg
      601 aatgttaacg tatttgataa ggatttgttg atcgacgcta tggaacatcc tgagaaatat
      661 ccgcaactta cgatccgtgt atccggatat gcggtaaact tcgtgaaact gactcgtgaa
      721 caacagttgg atgttatttc aagaaccatt aaccattcac tgtaagtgat aaaaggtaag
      781 atacattcat tagagagttt cgggaccgta gatggtcccg gaattcgctt tgtcgttttt
      841 atgcagggct gcccgttgcg ttgcctgtat tgccataacc cggatacttg gaacccgaaa
      901 gggaaggtga aatatcagat gactcccggc gagctattga cggaagtctt acggtataag
      961 agttttatcg cccgtggggg ggtgactgtt accggtgggg aacctttatt gcaacctgag
     1021 tttctgaaag agtttttccg tttgtgccaa gagcaaggtc ttcacacagc tttggacaca
     1081 tctggtttcg tatgtacctc aaaagcttgg gaagtcttgg attatgcgga cttagtgtta
     1141 ttggacatca aaaccttaaa cccggattta catccgttac tggcgggtgt caagcaggat
     1201 aatacgcttc tgttcttgga tgaattggag agacgtggca tagatacttg gattcgccat
     1261 gttattgtcc ccggatatac ggataatgac gagtggctgg aggcgttagc tcggtatgtc
     1321 agttcatata aagtggttcg caaagtggaa ttattgcctt atcatacgat gggaacctat
     1381 aaatatgagc aattaggtct cgattatccg ttaaaggggg tggaaccctt atcaaaagaa
     1441 cgtttagata atgcgaaagc gatattctct cgctacaaac cagaaaataa caaggcctga
     1501 tctctcgatc aagccttgcg attatgcaac agctaatcag ggaaaacaat ctaatccgat
     1561 tagcttttaa acgatagtaa taattttttg cctagactaa ccttgactat ttaatatgtg
     1621 tttgttttgt tatatgcaac aaatatctat ttttgtaagg agataaaaaa tgatatagat
     1681 cagaaaatga tgaatagtat gaagacacca caagaaatag ccgaaatgat atccaaacgt
     1741 ttttatccat tacccgatgc ggatttggat tctctggcct cgatcataga atcaagtaag
     1801 gctgcgaaag gagaattatt gttggataac ggacaaatat ctaaatattt atattatgtg
     1861 gaatcgggat tattacgcca gttttactat aagaataaac gtgatattac cgagcatttc
     1921 tcctgtgagg gaaatatcgt tatttgtata gccagcacat tccaaagaga accgacgcgt
     1981 ttattaatcg aggcattgga accgacggtt tatcaccgga tctctttttc ggaatttagc
     2041 gatttattga aaacatcttt agctattaat agattgtacc agaaaatact ggagtgggct
     2101 ttgatgcttt cgcaagagaa agcggactct tggcgttttg agtcggcaag aaaacgttat
     2161 ctgcgttttc aacaggaata tccggaagca gcgaaacgtg cttctgtcaa tcatatcgcc
     2221 tcgtatttat tgatgacacc cgaatctttg agccgggtgc gggcgggagt gttgtagtct
     2281 ctacctacat tcaggtatta ttgaggtgaa aatagcaatt tgtggtgatt tcacggttca
     2341 agtaatccat ctatatttgc gtcagtttaa aaaacgtaga agcaatgaaa atagaaaaga
     2401 ttacaggacg tgagatccta gattcaagag gtaacccgac agtagaggtt gatattttac
     2461 tagagtccgg cgttatgggc cgtgcgtctg tgccttccgg tgcgtccacc ggcgagaacg
     2521 aggctttgga gttgcgtgat ggcgataaga agcgttatgg tggtaaaggc gtattgaaag
     2581 cggttgagaa tatcaatacg attatcgctc cggcattgaa ggggatgtct gccttggatc
     2641 agataggtat cgatcatgcg atgttggctt tggacggtac gaaaactaaa tcgaaactag
     2701 gtgcgaacgc tatccttggc gtttctttag ctgtagcgaa agctgccgca aattatttgg
     2761 atattccttt atatagatat attggtggaa cgaataccta cgtgatgcct gttccgatga
     2821 tgaatataat ca
//
