LOCUS       c00256_gnlPro..         7046 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ELOGBILD_256.
ACCESSION   c00256_gnlPro..
VERSION     c00256_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:08:24
            Original ID  :: gnl|Prokka|ELOGBILD_256
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7046
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7046
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2789]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2789
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7046
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7046
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..547)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.6;
                     E-value: 6.5e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg256_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-29, bitscore:
                     91.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRPAAWLEDKEKPFWLRY
                     VLVPGYSDFEEDILALVEA"
     CDS             complement(561..2789)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg256_2"
                     /sec_met_domain="Gly_radical (E-value: 5e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             3390..4223
                     /locus_tag="ctg256_3"
                     /transl_table=11
                     /translation="MKSTFSVLFFVKKDKQKINGSYPIFVRVTIDGVASRFNSKLDVQP
                     KVWDGKAGKAAGRSAEATRINRLLDDINASLNTIYHELQRRDNYVTAEKVKNEFLGHSE
                     SHDTILNLFQKHNDDVKQLVGISKTIATYRKYEVTRRHLAEFIQSKYNLSDISIKEITP
                     MFITDFELYLRTTCKCGFNTTAKFMQFFKRIILIARNNGILIGDPFASYKIRLEKVDRG
                     YLSEDEIKIILKKKMVSERLEQVRDVFIFSCFSGLAYVDVANLKEDNIRKFFYVN"
     CDS             4290..4628
                     /locus_tag="ctg256_4"
                     /transl_table=11
                     /translation="MILEKYKGKLPDGKVLPIISNQKLNAYLKEIADVCGIKKNLTFHL
                     ARHTFATTTTLAKGVPIETVSKMLGHTNIETTQIYARITNNKISNDMQGLDKKFVGIEK
                     IYKEVSMK"
     CDS             4705..5211
                     /locus_tag="ctg256_5"
                     /transl_table=11
                     /translation="MDLQIIQNKIFEVRGCRVMLDFHLAELYQVETRALKQAVKRNLSR
                     FPSDFAFQLTKDEWQELITNCDKFPENIRHTPTPPLAFLEQGVAMLSSVLRSQTAIDVN
                     ISIMRAFVLMRQMSIGYEELLKRIEELEQSTDAQFSEVYQALTQLLSKPEPKPRKPIGY
                     RTYDE"
     CDS             complement(5233..5370)
                     /locus_tag="ctg256_6"
                     /transl_table=11
                     /translation="MWRFFRKPERFRFGTCPRRAVPLLKFVIEIHYFRELKRGITGDTP
                     "
     CDS             5469..5921
                     /locus_tag="ctg256_7"
                     /transl_table=11
                     /translation="MKTTEFTMPEITISYKDNVKASERVKILSSETSYSYLKPFYAECM
                     EHHEESHVMFLNRANKALGVSLISKGGMAETVMDVKIILQTALKVHASGIILSHNHPSG
                     NLRPSEPDKQITSKIKEACKVLDLHLLDHIILTEESYYSFADEGLL"
     CDS             6220..6753
                     /locus_tag="ctg256_8"
                     /transl_table=11
                     /translation="MEAVTLSEARVYVGTYARYNNGSLFGKWLDLSDYSDKDEFLEACR
                     ELHKDEQDPEYMFQDYENVPESLISESWLSGKFFELWDAIEKLSETQQEAFFVWCDHHN
                     SDISEEDADDLVSSFEDDYQGEYKDEEDYAYEIVEQCYELPEFAKTYFDYSAFARDLFM
                     TDYWMEDGFVFRCA"
ORIGIN
        1 gtgcttctac cagagcgagt atgtcttctt caaaatcgct gtatccgggc actagcacgt
       61 agcgtagcca gaaaggtttc tccttgtctt ccagccaggc ggccgggcgg atggtttgct
      121 cgttgcttct tccggtgagc gtctgatggc ggttggggtt gaattccttt atatccaata
      181 acacaagatc ggtcagtctg aataattctt ctacgtcttc gttccaaagt cctccgttgc
      241 tgtccagaca gacatggatg cctttttctt tcagttcgcg gaccagcggg acgagtgctt
      301 tcgcctgaaa cgtcggttcg cctccggaga aagtgattcc ccctcgtttc ccgaagaagg
      361 ggcgctggct cattgccatg cggacgattt cttccggtgg ggtaggcgtg cctccctttc
      421 ctgctatcgt atccggattg gcgcaataga ggcagcggaa gttgcatcct tgaagaaaaa
      481 cgacgagccg taagcccggc ccgtcgaatg ttcccatact ttcgtatgaa tgtacgttta
      541 tcatcatata gagtaataga ttacatacgt tcgtggaagc tacggctgat aacttccagt
      601 tggtgttcgc ggctcagctt cacgaagttt acggcatagc cggaaacgcg gatagtcagc
      661 tgcggatagt tttccggatg ctccatggca tcgtacagca tatcacggtt cagtacgttt
      721 acgttcaggt ggtgggcacc tttggtgaag taaccgtcca tcatcgttac gaggttctcg
      781 atacggtctt cgtccgtagc acccagtgat ttcggaacga tagagaaggt gttgctgata
      841 ccgtcctgtg agtcacggta acgaagtttc gctacagagc tcagagaagc gatcgcacca
      901 ttcttgtcgc gtccgtgcat cgggttggca cccggagcga aggcaacacc tttggcacgt
      961 ccgtcgggag tagcaccggt ctttttgccg tacatcacat tggaagtgat agtcagcaag
     1021 gagagggtag gacgggcatt cttgtaaaca ggcaatttct tcaattcttc gctgaagaag
     1081 tataccaggt ctacgccaag gtggtctacc ttgtcgttgt cgttaccgaa gcaagggaat
     1141 tctccttcga tgtcgaagcc ttctgtcaga ccgatatcat tgcggcgggc agttactttc
     1201 gcatatttga tggcagacag tgaatcgagg gcgatggaaa gtccggctac accgtaagcc
     1261 aggttgatgc gcgggttcgt atctacaaga gccatctggg ccttctcgta gtaatacttg
     1321 tcgtgcatat agtggatgat gttcatcgct tcgttgtata cgcgggcgat ttcggtcagc
     1381 acttttttgt agttgtccat tacttcttcg aacttcagcg tgtcactggt cagtacgggg
     1441 atgttcttca ccatgactgt accggtattc tcgcaacgtc cgccgttgat ggcaagcaac
     1501 agggctttgg ccaggttgca acgggcgccg aagaactgaa tctgcttacc tatttcctgg
     1561 tatgatacgc agcaggcgat tccgtagtcg tcggactgac gtacttcgcg catcaggtcg
     1621 tcattttcgt actggataga agaagtgtcg atagaaacct ttgcacagaa ttctttgaat
     1681 ccttccggaa gttccgggct ccagagtacg gtcaggttcg gttccggtga agggccgagg
     1741 ttgtacaatg tctgcaggaa acggaaggag gtctttgtca ctttggtacg tccgtcgttg
     1801 agacgtccgc ccagagattc ggttacccag gtcgggtcgc ctgcaaagat gtcgttgtat
     1861 gattgcatac gcaggtggcg gaccatacgc agtttgatga caaactggtc gatcagctct
     1921 tgcgcgaatg attcggtgat ggttcctttg ctcagttcat attccagata gatatcgagg
     1981 aaagaagaaa cgttacccag tgacatagcg gcaccgtctt gttctttgac ggcggcaagg
     2041 taagccatat atacccactg tacggcttct tgtgccgtgt aagcgggacg gctcaggtcg
     2101 agaccgtagt attcgcccat cactttcatg tctttcagtg ccttgatctg ttctgccact
     2161 tcttcgcgca ggcggatgcg ggcctctgtc atcggaccgg tgaggttgtg caaatcttcc
     2221 ttctttgctt cgatcagacg gtcgatgccg taaagagcca tacggcggta gtcaccgatg
     2281 atgcgtccgc gtgcataatt gtcgggaagt ccggtaagga atcccagcga acggaaggaa
     2341 cggatttctt ccgtatatac gtcgaatact ccgtcgttgt gcgtcttgcg gtagtgagtg
     2401 aaaatatctt ttacgcggtc gtccacttcc acgccgtttt cgtgacaggc tttgctgact
     2461 acgttgatac ctccgaaagg cttgatggca cgtttcagaa gttcgtctgt ctgcaggccg
     2521 acgatcagtt cgttttcctt gtcgatatat ccggccttgt gggaagtgat ggttgatacg
     2581 gtaacattat ctaatgagcg gacaccgttg ttttctcttt cttctgccaa tgcttcgagg
     2641 cagcggttcc atacggcttt ggtacgttct gtaggtcctt cgaggaatga agcatctccg
     2701 taatacggag tgatgttatg acttacgaaa tctctgacat tgatttcgct gctccaaaga
     2761 ccgtctttaa agatcttgtt taattccata aaatgataaa attagaggtt gtagttatcc
     2821 tattctttag agcgcacaaa gttacaatca tttttcatat aatcagcata aatatgctct
     2881 attttatgct tttcaacata aaatacctat taattgctat ccttaggaaa agattagatt
     2941 aaatttttag tgaaaactct tgcaagttta aaatatcttc gtacctttgc accgctttta
     3001 acgaaaagca cttctgataa ggaagttttg gagaggtggc agagtggtcg attgcggcgg
     3061 tcttgaaaac cgttgtaccg cgaggtaccc ggggttcgaa tccctgtctc tccgctgaac
     3121 taaccggaca aaaccggaca atgaacagcc aagtcccaca aattcaacga tttgtgggac
     3181 tttttttatc cccttatgtc tgccctaccg gacatcattt agaccctaaa agacaaagtt
     3241 agtaacccaa ctcgtacccc cgacaaaaga aaaggcaaaa ggggtacagt tagtctgaaa
     3301 ttggcgaact tctgtcgcta tttggcgagc ctgtataaac ttcactttga gctaattaac
     3361 gtagttttgt aaccaaaaaa agtgagttta tgaagagtac attttctgtt cttttctttg
     3421 tgaaaaagga caagcaaaaa atcaatggca gttaccctat tttcgtccgt gtcacgattg
     3481 acggtgtggc aagtcgtttt aattccaaac ttgatgttca acccaaagtt tgggacggta
     3541 aggcaggtaa agccgccgga cgttctgcgg aagctacccg tatcaaccga ttgctggatg
     3601 atataaacgc atccctgaac accatttatc acgaactgca aaggcgtgac aattatgtta
     3661 ccgccgaaaa ggtgaagaat gaatttttag gtcatagtga gagccacgac accattctta
     3721 accttttcca aaagcataat gatgatgtta agcagttggt cggcatatct aaaacgattg
     3781 ccacttatcg taaatatgaa gtgacccgcc gccatcttgc agaattcatc caaagcaaat
     3841 ataatctgtc ggatatttct ataaaggaaa taaccccgat gttcattact gacttcgagt
     3901 tatatttgcg taccacctgt aaatgcggct ttaatacgac cgccaagttt atgcagtttt
     3961 ttaaacgcat tatcctgata gcccgtaata acggcatctt gataggcgac ccattcgcca
     4021 gttataaaat acgtttggaa aaagtggata gaggttattt gtcagaagat gaaataaaga
     4081 taatcctcaa aaagaagatg gtttctgaac ggttggaaca ggtgcgtgat gtttttatct
     4141 tttcctgttt cagtggtttg gcttatgtag atgttgcaaa cttaaaggaa gataatatcc
     4201 gtaagttctt ttacgttaat taatggttaa ttccgaaact ccaaaaaaca tatactgatg
     4261 ttaatgtttc ttttctggat attcctaaaa tgatattgga aaagtacaaa ggtaaattgc
     4321 ccgacggaaa ggtacttccc attatcagca atcaaaaact taatgcgtat ttgaaagaaa
     4381 tagcggacgt atgcgggatt aaaaagaact tgacatttca ccttgcaagg cacacatttg
     4441 ccaccacgac cacgcttgca aagggtgtcc ctattgaaac agttagtaag atgttgggac
     4501 ataccaatat agaaacgaca cagatatacg cccgcattac caataacaag ataagtaacg
     4561 atatgcaggg acttgacaag aaatttgtcg gcattgaaaa aatctacaaa gaagtatcta
     4621 tgaaataggt atatagggga acttatcaca atttgtgata agtcccttta tgctttcaat
     4681 tactcaccat taaataataa cattatggac ttacaaatca ttcaaaacaa aatctttgag
     4741 gtcagaggtt gccgggtcat gctcgatttt catttggcag aactttatca ggtggaaaca
     4801 agagctttga aacaggcagt taaaagaaac ttatcccgtt ttcccagtga ctttgctttc
     4861 cagctaacaa aggatgaatg gcaggaactt atcacaaact gtgataagtt ccctgaaaat
     4921 atccggcaca caccgacccc acctctcgca tttttggagc aaggtgtcgc catgcttagt
     4981 agtgtcttac gcagtcagac tgccatagac gtgaatattt ccatcatgcg tgctttcgtc
     5041 ctcatgcggc aaatgtcaat cggttacgag gaacttctta aacgcatcga agaactggag
     5101 cagagtacgg atgcacaatt cagcgaggtg taccaagcat tgacccagtt attaagcaaa
     5161 cccgaaccga agccacgcaa accgatagga tatagaacct atgacgaata acggtttaag
     5221 gtattcggtt gtctaagggg tgtcaccagt gatacccctt ttcagttccc taaagtaatg
     5281 tatctcaatc acaaatttta gcaaaggtac agcccggcgg ggacaagtac caaatctgaa
     5341 ccgttccggt tttctaaaaa atctccacat ctacgggtcg tattttttga aaagatatgg
     5401 ttgaagtctc cgccaaactg tgggtgtccg gcaaaatttc ttattgttta atttaaaatt
     5461 cagtagttat gaaaacaaca gaatttacca tgccggaaat cactatctct tataaagaca
     5521 atgtaaaagc atctgaaaga gtgaaaatac tctcatccga aacgtcctat tcttatctga
     5581 aaccgttcta tgcggaatgt atggaacacc acgaggaaag ccatgtaatg ttcctcaatc
     5641 gggctaacaa ggctttgggt gtttccctta tctctaaagg cggcatggct gaaacggtta
     5701 tggacgtgaa aatcatcctg caaacagcct taaaagtaca tgcttcgggg attatcctct
     5761 cacataacca cccatcgggc aacctacgtc cgagtgaacc ggacaaacaa attacctcaa
     5821 aaataaaaga agcctgtaag gtcttggatt tacatctgtt agaccatatc atcctgacag
     5881 aagaaagtta ttatagtttt gcagacgagg ggcttttata agcccttttt ttactctctc
     5941 attacccaat acaaaaagcc tgatggcttt tacttctatc ctctcaaaca agagaaaaat
     6001 tttccctaaa actgattgga acattgtcaa aggtatatcc gtgcctgtta ctgttcaaat
     6061 tattttttag aaatctccac acctacgggt agtatttcat aaataataat ttgccctgta
     6121 ccattcccaa acctttgaaa agacaatgtt cccattcagt taaaggaaaa atttatgtgg
     6181 tgggcgacaa gcgatgtaaa caattaaaat tcaaaagtca tggaagcagt aacattatca
     6241 gaagcaagag tttatgtagg cacatacgcc cggtataaca acggttcact tttcggcaag
     6301 tggttagacc tgtccgacta ttcggataag gacgagtttt tggaagcgtg ccgggaactg
     6361 cacaaggacg agcaagaccc ggaatatatg tttcaggatt acgagaatgt acccgaaagc
     6421 ctgatttccg aaagctggtt atctggtaag ttctttgaac tttgggatgc cattgagaaa
     6481 ctaagcgaaa cccagcagga agcgtttttc gtatggtgcg accaccacaa cagcgatata
     6541 agcgaggaag atgcggacga ccttgtttct tcttttgagg atgattatca gggggaatat
     6601 aaggacgagg aagattacgc ctatgaaatc gtagagcaat gttacgaact gccggagttc
     6661 gcaaagacct actttgatta ttcggctttt gcccgtgacc tgtttatgac tgattactgg
     6721 atggaagacg gttttgtttt ccgttgcgcc tgattactaa ccgggtggag caatccaccc
     6781 gcaacacacc aagacaatga aagtatataa taagatagcg acttttttac gatggttcat
     6841 acttgctttt atcatctatc aggcagccac cagcaccgcc gggacatgga tagctttaat
     6901 cataggcttt tttattctcc ggttctttct ccggttgttt atctccgccg tttacctgtt
     6961 ttgtatggcg tttattttca ttgtgttgct tccgctctta cttctctcac acctcgttat
     7021 aaattttaac gttctggaac ctgtaa
//
