LOCUS       c00186_gnlPro..         4306 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CIIIGFFF_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:51:26
            Original ID  :: gnl|Prokka|CIIIGFFF_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4306
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..447)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74; E-value:
                     4.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-24, bitscore:
                     74.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTEC"
     protocluster    1..4306
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2686]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2686
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4306
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4306
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(434..2686)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_2"
                     /sec_met_domain="Gly_radical (E-value: 3.8e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             3533..4132
                     /locus_tag="ctg186_3"
                     /transl_table=11
                     /translation="LYEEQRLTIPATGKHDWSDWWIVKKSTVFKTGTKKRECWICDKTQ
                     TVSIPKLKPFVKLNKKTISLTAGKSYRLKASYAKGDSVKKCKSSNKKVATVSKKGVISA
                     RQAGKVRITIYTKSGKKATCKVTVTAKKKAAKKSSSNKSGSGSGSSKTNGSTVYWTPGG
                     AVYHRTPNCPTLSRSRTIYHGSISSCPKNRGCKVCF"
ORIGIN
        1 acattcagta tcaatatgtt tgatatccac taataacagg tctgtatatt tcatcaattc
       61 ctgaaattta gaaaagaaag gttcctctct tgtaaaaggt cctccagcag tatcaataac
      121 agtgtggatt cctttttctt ttgccttttt aaaaaactct agcataaaat caatctgtaa
      181 aagaggctca ccaccactta cggtaatacc gccgctattt ttccagtaag tacgataacg
      241 gagtgcctga tttaacagct catccgcact tctccacacc gcaccatttt cctctgtcat
      301 cttccatgta tctggattgt gacagaactg acatcgcaaa ggacaaccct gtaaaaaaac
      361 aataaaacga actcccggcc catccaccga accaaaactc tccgtagaat gaacaagagc
      421 catcgcattt ttattacatt ctgtcatggc atgttctggc aattacatct aactgctgtt
      481 cttttgtcag agagatgaat ttaactgcat aacctgaaac acggatcgta aagtttgcgt
      541 attcttcttt ttccggatgt tccatagcat caatcagttt ctcaacacca aatacattta
      601 cgtttaggtg gtgagcaccc tgtgcgaagt agccgtccat aacatttact aagttattga
      661 tacgttcttc ttcggaatga ccgagtgcat cagggttgat tgtctgtgta ttggaaatac
      721 catctaatgc ccattcgtaa ggaagttttg ctacagagtt taaggaagct aaaagtccgt
      781 tctgctcagc accataactt gggtttgctc ctggggaaag aggttctccg gcctttcttc
      841 cgtctggaag ggaaccagtt gccttaccat atactacgtt tgatgtgata gtaaggatgg
      901 aagttgtagg ttcagaatca cggtaagtat ggaatttttc aagcttcttc ataaatgtct
      961 gaagtaacca tacagcgata tcatcagcgc ggtcatcatc atttccgtat ttagggaagt
     1021 cgccagttgt ctcataatca acaacaagtc cttcttcatt gcggactgtt tttactttgg
     1081 catatttaat cgcacttaag gaatctacta catgtgagaa tcctgcgata cctgtagcga
     1141 atgtacgacg tacatttgta tcgattaagg ccatcaaagc atattcatag taatatttgt
     1201 catgcatgta ctggatgaga tttaatgtat ttacatataa acctgctaac cagtccatca
     1261 tgatatcata tttgtgcatt acttcatcat agtcaagata ttcggaagta atcggtttat
     1321 attccggtcc aacctgctgt ccggtctttt catccacacc accattgata gcgtaaagga
     1381 gacattttgc aaggttagcg cgagcaccga agaactgcat ttccttacca gtctgtgtag
     1441 cagaaacaca gcagcagatg gagtaatcat cgccccatac tggacgcatt acatcatcat
     1501 tttcatactg gattgaactt gttgtaatag aaatatgagc tgcgtaatct ctaaatgctt
     1561 ttggaagacg ctcagagtaa agtactgtga ggtttggctc tggggatggt cccatatttt
     1621 ctaatgtgtg gaggaaacgg tagtcagttt ttgtaaccat ggaacgaccg tcctgtccaa
     1681 gtccgccaac ttcaagtgtt gcccatacag ggtcaccgga gaagagctgg ttgtaagctg
     1741 ggatacgtcc gaacttaacc atacggaact tcatagtcat atggtcgatt aattcctgcg
     1801 cttctttctc tgtaaggatt cccttatcca tatcacgttt gatataaatg tcaaggaaag
     1861 tagcgatacg tccgatactc atagcagcac cgttctgtgt cttgattgct gcaaggtatc
     1921 cgaaatacgt ccactgaaca gcttcctttg cgttagaagc aggttcggaa atatcatagc
     1981 catagctttc agccataact ttcatatctt ttaatgcttt aatttgcatg gagatttctt
     2041 ctcttaaacg gataacgtca tctgtcattg ttccgtcacc gcagtttgcg aaatcttttt
     2101 gcttgtcagc aactaatagg tcaataccgt aaagagctac acgacggtag tcaccaacga
     2161 tacgtccacg tccgtaagta tctggaagac cagtaatgat cttattgtga cgagctttct
     2221 tcatttcagg agtataagaa tcaaatactg cctggttatg tgtcttatga taatctgtaa
     2281 aaatcttgtg gaattctgga tttggtgtgt aaccataagt tgtacaagct tcttcagcag
     2341 tacggatacc accaaatggc ataaatgcac gttttaaagg tttatctgtc tgaagaccta
     2401 cgatctgttc tttctctttt aaagactcag aaatgtaacc cggtttatgg gaagtaaggc
     2461 ttgatacgat atcagtatcc atatcaagaa caccaccctt ttcacgctct gctttctgca
     2521 gcttctgcag ttcgccccac agtgtgtcag tagcttcggt aggaccttcg aggaaagaag
     2581 catctccatc atatggagtg tagttatcct ggatgaactc tcttaaatta atttcttctc
     2641 tccagattcg tccattaaat ccctgccatt gttctttttg taacatctta attgacctcc
     2701 ctgtttgtta aaaaaatcct taagtcaagg ttttgaaata tgtttgctaa aaaatcaaca
     2761 aaattgttca gatgatttac tgtaattagt tttaacttat ttaagtttta atcatctaac
     2821 aacattatta acatgatttt tggcaatcgt caaacaattt tttgatatta tgtgttcttt
     2881 tttttgaaca tatacacaaa aatgcttgta cagcctttaa aataaagggt tttagagcgt
     2941 tgataaaata ttatcaaatc gtacaaatgt actgatataa gtacaataat gacaagcata
     3001 caactataat tttatttttc gctctttaga gcatgtttgg aaaaacatct tctttagcac
     3061 atgacttaat aacgatttaa agttacttct tatcagggat attggataga atattatctt
     3121 atcagaaata aaaaagccag catccagaca agaattgccc agacggtcgg ctataagagt
     3181 cttatacttc atgtggttca ttccacttac ttccgtcagt cgtataagta tcctaactat
     3241 aaacaaaata gaacaaaatg tcaaggggtt actatttaat tcattaagag aactacagac
     3301 gaagccattg cccccaataa ggcatatttg tgattttagt cgcggcgtgt tgaatgttcg
     3361 ctttatgcgc tcgcatccaa cacttgctcc gagaccacaa atatgcctta ttgggggcaa
     3421 tggcttctgt gctgtggctg gagaggatga attaaacagg aggtggggat ttggggacag
     3481 aagctgatgt attctgctta cccgagaata tacgcaaaag acaaggaggt ttttgtatga
     3541 agaacaacga ctcaccattc ctgctaccgg aaagcatgac tggagcgact ggtggatcgt
     3601 aaaaaaatct acggttttta aaacaggaac aaaaaaaaga gaatgttgga tctgtgataa
     3661 aacacaaacg gtttctattc ctaaattaaa accatttgtg aagttaaata agaaaactat
     3721 ttccttaact gccggaaaat catatcgttt aaaggcatct tatgccaaag gcgattctgt
     3781 aaaaaaatgc aaatcttcta ataaaaaggt tgctactgtc tctaaaaaag gagtaattag
     3841 cgctcgacaa gctggtaaag ttcgaattac tatctacaca aaatccggta aaaaggcaac
     3901 ctgcaaagtt actgttactg cgaaaaagaa agccgctaag aaaagttcct ctaataagag
     3961 cggttctggt agtggttctt caaaaacaaa cggcagtact gtttattgga caccaggcgg
     4021 tgcagtttat catcgcacac caaattgtcc gactttgagt cgatcaagaa cgatttatca
     4081 tggttctatt tctagttgtc cgaagaaccg cggatgcaag gtgtgctttt aaattttgat
     4141 ttgcgcagca aaggagacac ccacagggaa tataatatcc tatgggctgt gttttgtctg
     4201 cctaaacgaa ttcagaattt attccaactg gttttctgag ctgccaacgc acagaactcc
     4261 tgaagataaa taaagcatat ttgtggcctc ggagcaaggt ttggat
//
