LOCUS       c00028_gnlPro..         6464 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JENKNIGD_28.
ACCESSION   c00028_gnlPro..
VERSION     c00028_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:12:54
            Original ID  :: gnl|Prokka|JENKNIGD_28
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6464
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6464
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2768:6258]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2769..6258
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6464
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6464
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             73..780
                     /locus_tag="ctg28_1"
                     /transl_table=11
                     /translation="MNKLLSTLILSAGMSLSATAATALLDSSIVGYSDFASQTADFGTR
                     KDSVSITSDDSNLTLSGIIFNGYGANNRDVMSVTMVLDLSKINTPETYTALFNAKNSSV
                     SWGVGLNTDRKLQGLWNNTSYNGPVVDTPLGTEGSLTISVVTGDLGTRIYFGDSATYYS
                     NSGLKFGNQQIDQILMDAGLAESIEQLYIHDSLLSQEQVGQLMSEIASIPEPATASLGL
                     LGLAALMMRRRRA"
     CDS             1211..1444
                     /locus_tag="ctg28_2"
                     /transl_table=11
                     /translation="MNLQKQQLLLRLFRYFKSRKGEPRFFNKRNICLLAAAGAYILTPD
                     EFLFDGIGRLGLLDDAAVILGAVLTVFFPDGD"
     CDS             complement(1478..2263)
                     /locus_tag="ctg28_3"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(2296..2568)
                     /locus_tag="ctg28_4"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             2769..3470
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg28_5"
                     /sec_met_domain="SucD_FumR (E-value: 2.9e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             3492..5450
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg28_6"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.7e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.3e-38,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             5491..6258
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg28_7"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 75.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.4e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.8e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     ESLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 cggattccaa attcgttatt caagcacaag tacatacatt gaaccttgaa tcattttatt
       61 aagatactga ccatgaataa attattatcc accctgattc tttcggccgg catgagccta
      121 agcgctaccg cggctaccgc tctgctggat agctccatcg tgggatattc ggactttgcc
      181 tcccaaacgg cggatttcgg cacccggaaa gattccgttt ccatcacgtc tgacgacagc
      241 aatctgacgt tgtccgggat aatcttcaac ggatacggtg ccaacaaccg cgacgttatg
      301 tctgtgacga tggttcttga cctgtccaaa atcaacactc cggagaccta taccgctctg
      361 tttaacgcca aaaacagcag tgtaagctgg ggcgtgggcc tgaacacgga ccggaaactc
      421 cagggattat ggaacaacac ctcatacaac gggcccgtcg ttgacacccc gctgggcacg
      481 gaaggttccc tgaccatttc cgtagtcacc ggagatcttg gcacccgcat ctattttgga
      541 gacagcgcta cgtactattc caacagcggc ctgaaattcg gcaaccagca aattgatcag
      601 attctcatgg acgcaggact ggctgaatcc atcgaacagc tctacatcca cgactccctc
      661 ctttcccagg aacaagttgg tcaactgatg agtgaaatcg ccagcatccc ggaacccgcc
      721 acggcttccc tgggcctgct gggactggcc gcgctgatga tgcgccgccg cagggcataa
      781 gcctgtaccg tccgcctccc tatattcaaa ggccggctca caaaatcgtg agccggcctt
      841 ttccttatcc gttcaaacat ctgccggaaa aaacaggggg gaagaagctt ccatttgctg
      901 cggcaacctc acggccagca cttgacgggg aaatggcatt caggctcccc cgggagggtc
      961 atttcaagag cttccggccc cgccccactt ccgtacacgc ccctttttcc catgccggga
     1021 cagccggaac cggagcatgc aaagcccggc ttccctcagc atccggaaca gcccatcctt
     1081 tcagcaatcc atttccccgc acggtccgga tcgctgcggt tccgtccgtc ggcaacagac
     1141 tccttccccg aaacagggaa aaggagtggg aaaatggctg agcttgaacc tttatccccc
     1201 gttcccgtcc atgaaccttc aaaaacagca gctgctactg cgcctgttcc ggtatttcaa
     1261 aagcagaaaa ggagaaccgc gttttttcaa caaacggaat atatgccttc tggccgccgc
     1321 cggcgcctac atcctgactc ccgacgaatt cctgtttgac ggaatcggca ggctgggcct
     1381 tctggatgac gcggcggtca tcctgggcgc cgtcctcacc gtctttttcc cggacgggga
     1441 ctagagggcc gtaccctccg ccgcgccgcg ggcggaacta acgggacgac actttccgga
     1501 acttcgggga ctgtcccatg aatgtctccg gattttccag gagaagacgt tccgtttcct
     1561 gccgggaaaa accgcgcctg cgcagctcgc aggccagggc cgtcagcgcc aggggatcgg
     1621 aatcccccca gtccgccgcg gaattggccc agacacggtc cattccgtac aattccagaa
     1681 tatccgccgc ccgcttggga ttcagcttgc tggtgggata aagggtcagc ccggcccagt
     1741 agccggcctc cttcaccagc gggaaggtat gttcctcaca gtgatccacc agcacgcggc
     1801 aagggtccag atccggacgg tttttcaggg cggaaagaat cattacggtc cccttaagct
     1861 tgtcttccag atggggagtg tgaatcagca caagctggtt gaggcgcacc gccagatcca
     1921 gatgctcttc aaaaatagtc atttcatggg aagtattccg gttcagccct atttccccga
     1981 tgccgaggac attgggccgg tcaatgaact ccggaatcag cttcatcaca tcccgcgcga
     2041 aagccacgtc ctgcgcctcc ttgggattga tgcacagcca tgtgtaatgg tccatgccga
     2101 acttggccgc cctggcgggt tcgcattccg taagctgtac gaaataatcc cggaaaccgt
     2161 ccgcggacga acggtcgtaa ccggcccaga acgccggttc ccctactgcc aggcagccgg
     2221 cctgcgccat ggcccggtat tcgtccgtgg tgcggcacac catatgtata tgaggttcta
     2281 tgaaagccat cattcttatt tgctgaggtt cggggctccg caggccgctt tggctgcggc
     2341 ggtttccggc acggtgtcat ccctgtagga aagggcttcc agaatggccc ggacgcgtcc
     2401 ggggccggat gcggacaaga ggggaagaac atccatcagg gcatggtagc ggaaatccgc
     2461 ctccccttcc ccgcggtcca cccggtccag aaatgcggcc acatccagaa cgccggcgcg
     2521 cgccgctgcg aaatccgcgt ccagcacctc ctttttactt cttttcatgc acagacgata
     2581 cgcctatgcg gaaagcctcg tcaagccgga acaggcaaca aaatgataac cattccaaac
     2641 tgataaacag caacctcttt ccccggtttt tcaaaaatcc gaaaagcgcc ggaaattcaa
     2701 gaagaggctt gccatccggg cttgaaccgg gcatcctgtg aagacgcatt tatttcatta
     2761 gagcgcctat gaatgcacta gtaaaaacca tatgtacatt cgtgacatcc tccatcggcc
     2821 gcaaaatcat tgtggctctg acggggttat gtttggtctt gttccttgcg ggacacctgg
     2881 caggcaacct tctcatcttc ggaggccctg aatggatcaa cacctacgcc cacggcctgc
     2941 actccatgcc ggaagcagcc ctctggggaa tccgcgcggg gcttgcggtg atcttcatca
     3001 tccacgtctg gctgaccatc cagctgaagc tggaaaacca tgcggcccgc gaaccctacg
     3061 tgttcaagaa caccattaag gcaacgctct cctcccgcta catgatctat accggtttga
     3121 ccgtcctggt gttcctgatc taccacctgt accagtacac cctgcgcgtg ggatacgacc
     3181 ccgcccagtt caccaccttt atttccgacg gtacggtgga aacctttgac gtgtacaaga
     3241 tgatcgtgac cggattctcc aacgtatggt gctccgcctt ctacatcctg gccgttctga
     3301 tgctgttcag ccatctgcgc cacggggtac agtccatctt ccagactgtg ggcgtcgatt
     3361 cccgcaaaat ccggcccttc tacaacttta tcgccatcgc ctacggcgcg attatctgcc
     3421 tgggcttcat ctccgtgccg gtctcggttc ttctgggtat catcaaataa ccatcaactc
     3481 ctctcaaagt catgattaat tttcccgtaa gcgactggat gccggatgcc aaaattccct
     3541 ccggccctat tcaggacaaa tggtccaagt acaaacttga gtccaaactg atcaatccga
     3601 acaacaagcg caagtacacg gtgcttgtgg taggttccgg cctggctggc gcctccgctg
     3661 ccgccacgct tgccgaactt ggctacaacg tcaaatgctt ctgctaccag gacagccccc
     3721 gccgcgccca ctccattgcg gcccagggcg gcattaacgc agccaagaac taccagaacg
     3781 acggcgactc cgtttaccgc ctgttttacg acaccgttaa aggcggcgac ttccgcgccc
     3841 gtgaagccaa tgtctaccgt ctggcggaag tctcctccaa catcattgac cagtgtgtgg
     3901 cccagggcgt tcccttcggc cgtgaatacg gcggcctgct ggacaaccgc tccttcggcg
     3961 gcgcccagct caagcgaacc ttctacgcac gcggacagac gggccagcag ctcctgctgg
     4021 gtgcctacca ggccctggaa aaggaagtgg ccaagggaca cattgaaatg ttctcccgcc
     4081 atgaaatgat ggatctggtg ttggtggacg gcgaagccaa gggcatcgtc acccgcaatc
     4141 tggttaccgg cgaaatcaaa tcctacgcag gggataccgt cctgctctgc acgggcggct
     4201 acggcaacgt gttcttcctg tccaccaatg cgatgggttg caacgtcact gccgcctaca
     4261 aagcctacaa gcgtggtgcc ttcttcgcca acccctgctt cacgcaaatc caccccacct
     4321 gcattccggt caaaggcgac taccagtcca aactgacgct gatgtcggaa tccctccgca
     4381 acagcggccg catctgggtg cccaagtccc gtgaaaccgc agaaaaaatc cgtaaaaagg
     4441 aaatcaagcc ttccgatgtt cccgaagaag aacgggacta ctacctggaa cgcaagtatc
     4501 cctccttcgg caacctggct ccgcgcgaca tttcctcccg cgccgccaag gaagcctgcg
     4561 acgacggccg cggcgtggcc gtcaccggca agggcgtctt cctggatttt gcggatgaaa
     4621 tcaagcgcat gggccgcgaa tggattgacg gccaatacgg taatctgttt gacatgtacg
     4681 aagaaatcac ggacgacgac ccccatgaaa cgcccatgat gatctacccc gcctcccact
     4741 acaccatggg cggcctgtgg gtggactaca acctcatgtc caacgtgaag gggttgcacg
     4801 tcctcggtga agccaacttc tccgaccacg gcgccaaccg cctgggagcc tccgccctga
     4861 tgcagggcct gtctgacggc tacttcgtca ttcccgccac gttgcccgct tacctgacga
     4921 ccaccaagcc cggtgccgtc accaccgccg ccccggaatt caaggctgcg gaagacgaag
     4981 tgaaggaacg catcaacaag ctcctgaatg tgaagggcac caagacggtg gactccttcc
     5041 accgggaact gggcctcatc atgtgggaag actgcggcat ggcccgcacg gaagaaagcc
     5101 tgacccacgc cattgaacgc attccccagc tccgcaagga attctgggaa aacgtgcgcg
     5161 taaccggcac ggcggacggc atcaacgcgg aactggaaaa agcctgccgc gtggccgact
     5221 tcctggaatt cgcccagctg ctctgctatg acgccctgaa ccgccaggaa tcctgcggcg
     5281 cccacttccg cgtggaatac cagctgccgg acggcgaagc caagcgcaac gacgatgaat
     5341 acgcctatgt agccgcctgg gaatacactg gcgtggacag cctgcctgtt ctccacaagg
     5401 aaccgctgac cttcgagaac gtgcaccttg ccatccgcag ctacaagtaa agatagccaa
     5461 tacattaaca ctcaaaagaa agattataac atggctaaaa cactcaatct cactctcaag
     5521 gtctggcgcc aggaaaaccg ggaagccaag ggccgcattg agacctatgc cgccaaggac
     5581 attccggtgg acgcctcctt cctggaaatg ctggacatcg tcaatgaaag cctggtcaag
     5641 gaaggcaagg aacccatcca ttttgaccac gactgccggg aaggcatctg cggcatgtgc
     5701 tccctgacca tcaacggcat tccccacggg ccggaacgcc aggtgaccac ttgccagctt
     5761 cacatgcgca agttcaagga cggcgatacc gtctggattg aacccttccg cgcgaaggct
     5821 ttcccaatcc tgcgggacct gatggtggac cgctccgccc tggaccgcat catcgccgcc
     5881 ggaggctata ttgacgtgcg taccggttcc gcgccgaacg ccaacaacat cgccgtggaa
     5941 aaagtgaagg cggacgccgc ctttgacgcg gccgcctgca tcggctgcgg cgcctgcgtg
     6001 gcctcctgta aaaacgcctc cgccatgctg ttcacctccg ccaagatcgc ccatctcaat
     6061 ctgctgccgc agggccagcc ggaacgttcc aagcgcgtcc tggccatgat gaagcaaatg
     6121 gaagcggaag gcttcggcaa ctgcacgaac cagtatgaat gtgaagctgc ctgcccgaaa
     6181 ggcatcagcg tggacaacat cgcccgcctc aaccgcgact tcatgcgggc caacgccaag
     6241 gaaggtctct gtttctaaag aagattcctt cttaagacgc tctgtaaaaa ctcccggcgg
     6301 catcggctgt cgggagtttt tttcattatc aggtttcctt gaaccaggaa taaaatatca
     6361 gacagttgct tcctcatcgt cgcgcagcgt aaaaaataaa tgttacactg gaaggcaccc
     6421 agtaatccag gaaaccgtac ttggaaatgc cggacgaaac ctaa
//
