LOCUS       c00118_gnlPro..         7536 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EOPLJNPH_118.
ACCESSION   c00118_gnlPro..
VERSION     c00118_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 19:54:34
            Original ID  :: gnl|Prokka|EOPLJNPH_118
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7536
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7536
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3558:6550]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3559..6550
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7536
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7536
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..230
                     /locus_tag="ctg118_1"
                     /transl_table=11
                     /translation="QHEYNVEIRLEKLPYEYIRWIENTEIDLENLRGTSDMKKIKDLKG
                     RPLLLFINSWSVGMTLERNEGLVLSEFGRA"
     CDS             506..2152
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg118_2"
                     /sec_met_domain="Radical_SAM (E-value: 7.9e-19, bitscore:
                     57.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEKEYRLKTLFWEATLRCNAFCEFCGSRCGEMSCNEISGEKIEK
                     VFEDIADAYNPGSIMINVTGGEPILRKDLFEVMAKASAMGFPWGMVSNGSLIDKCIIQK
                     MKQSGMKTISISLDGMEETHNRLRKIENGFQKTVQAIKDLKQAAFLNQIEVTTVVNHYN
                     IEQLEEMYSMIKSFGIDSWRVAVADSIGRAKENQKLLLGKREFQQYCTFLDRHRFDTEL
                     RIITSCSHYLGKNDNKYRANPFSCETGKSVASILANGDIFVCPNVERRKELIQGNVQKD
                     NFVEVWENKFEFFRSETRCQSKKCKTCKDWESCRGDSLHTWNFEKKEPSFCIKDYMEDI
                     FYSKELPMELIKKMKSQVKDRMIGIKISSKSQTDQCVYFSPNASEELFYYFHFGKKHPR
                     NMDELMAGLAGHVINDNIVLVEFIVPIELDTRSSDKAVFSNNSYNKLQEELKIMNKARE
                     NCDEELQLFSSELQLVGTIHSHPGNLRTIMSIPDMGLHKYLSEHIDTYKTTTILNAQKR
                     QIASYGDTEFCSADIIFLEAEQQIARWNLVK"
     CDS             2178..3098
                     /locus_tag="ctg118_3"
                     /transl_table=11
                     /translation="MEEYTQRLTESSWDNKEWICSICGRQNKEEFKYCPECGEKKPDHE
                     ETQTEITIKKLKRKGFENCEQLFRLLIHVEDEEGNLLGSYNMTLHGYATKQELLNLLED
                     YLGYKENHRAPKKYHLEIQDWPYHSLDSAYETKEDFHVCELYSAEWNAVRKEYGEKRTD
                     HEETWEEIRIKQKDYANCEQKYKVLVHLEDEEGNPLGNYNVMLCEYDTEKELIDLLEDY
                     WGYKKNIYKEYYLKVIDWPHNPGPSEWWPGSSLHVCEMEEREFRVIRKKYKEDDGPMPC
                     LYGCPNSRDIEEKKEVETKVEIVYF"
     CDS             complement(3559..4311)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg118_4"
                     /sec_met_domain="Radical_SAM (E-value: 3.1e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(4298..6550)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg118_5"
                     /sec_met_domain="Gly_radical (E-value: 6.3e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.4e-267, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
ORIGIN
        1 tgcagcacga atacaacgta gaaatccgcc ttgaaaaact tccatatgaa tatatccgct
       61 ggattgaaaa tacagagatt gaccttgaaa atcttcgtgg aacatcggat atgaagaaga
      121 ttaaggattt gaaaggtcgt ccattgttac tgtttatcaa tagctggagt gtgggaatga
      181 ctctggagag aaatgaagga cttgtactct cggaattcgg aagagcctag agcgtgtttg
      241 aaaaatcatt ccagcaatct gcacacccca atttacggta tattttgtct tcattcggtt
      301 gccgtagccc gctacgccgc cttcattcag gcgaaatctc ccgcaaattg tgatgcacat
      361 cttgcagaaa gcatttttca aacacgctct agaatttctg atttgttgaa ttggcaaaac
      421 tgacgcccac agagaatatt gtattccctg taggcgtctt tcttgcctgc ggcaaatcaa
      481 aatttttacc aaaaaggaga ggtatatgaa agagaaagaa tatcgtttaa aaacactatt
      541 ttgggaggca acattacggt gcaatgcctt ttgcgaattt tgcggaagcc gttgtgggga
      601 aatgagctgt aatgagattt caggtgagaa aattgaaaaa gtgtttgagg atattgcgga
      661 tgcatataat ccaggaagca ttatgattaa tgtaactgga ggggaaccga ttttgagaaa
      721 agatttgttt gaagtgatgg caaaagcatc tgctatggga tttccctggg gaatggtttc
      781 taatggttcg cttattgata aatgcattat acagaaaatg aagcaaagtg gaatgaagac
      841 aatatctata agtttagatg ggatggaaga aacacataat cggttaagaa agatagagaa
      901 tggatttcag aaaactgttc aggctataaa agatttaaag caagctgcgt ttttgaatca
      961 gatagaggtt acgacagtgg taaatcatta taatattgaa caattagaag aaatgtattc
     1021 tatgataaag tcattcggga ttgattcctg gagagttgca gtcgcagata gcataggaag
     1081 agccaaagaa aaccagaaat tacttttggg aaaaagagag tttcagcaat attgcacctt
     1141 tcttgaccgg cacagatttg atacagaatt gcggataatt actagctgta gtcattatct
     1201 tgggaaaaat gataataaat atagagcaaa tcctttttct tgtgaaacgg gcaaaagtgt
     1261 agcatcaata ttagcgaatg gagatatttt tgtttgcccg aatgtggaga gaaggaaaga
     1321 attaattcag ggaaatgtac agaaagataa ttttgtagag gtatgggaaa ataaatttga
     1381 atttttccgt tcagaaacaa gatgtcaaag taagaaatgt aaaacttgta aagattggga
     1441 atcctgtcgt ggagatagtt tgcatacatg gaattttgag aaaaaagaac cttctttttg
     1501 tataaaagat tatatggagg atatttttta ttcaaaagag cttccgatgg aattgattaa
     1561 aaaaatgaaa agtcaggtca aagacagaat gataggaata aaaatcagtt cgaaatcaca
     1621 aacagatcaa tgtgtgtatt tttctccaaa tgcatccgag gaactttttt attattttca
     1681 ttttggaaaa aagcatccaa gaaatatgga tgaacttatg gcgggattgg caggacatgt
     1741 tataaatgat aatatagtat tagtagaatt tattgttccg atagaattag atacgagatc
     1801 ttcggataaa gcagtatttt ctaataattc ttataataag cttcaggaag aattaaaaat
     1861 aatgaacaaa gcaagagaaa attgtgatga ggaacttcag cttttttctt cagaattaca
     1921 gttagtagga acaatacata gtcatccggg taatttaagg acgataatga gtattccaga
     1981 tatgggcctg cataaatatt taagcgagca tatagacaca tacaaaacga ccactatttt
     2041 aaatgctcaa aagcgacaga ttgcttctta tggggacacg gaattttgtt cagccgatat
     2101 tattttttta gaggccgaac aacaaatagc aagatggaat ttggtaaaat agtattataa
     2161 agtcagggag tgcaataatg gaagaatata cacaaagatt aacggaatct agctgggata
     2221 ataaggaatg gatatgtagt atttgtggaa gacaaaacaa agaagagttt aaatattgtc
     2281 ctgaatgcgg ggagaaaaaa ccagatcacg aagaaacaca aacagagata actataaaga
     2341 aattaaaacg aaaaggcttt gaaaactgtg aacaattgtt tagacttctt attcatgtcg
     2401 aagatgagga gggtaatcta ttaggaagtt ataacatgac gctgcacgga tatgccacaa
     2461 aacaggaatt gctaaatttg ttagaggatt atctgggata taaagaaaat catcgtgccc
     2521 ctaaaaaata tcatttagag atacaagatt ggccatatca ttctttagat agtgcatatg
     2581 agacaaaaga agatttccat gtatgtgagc tctacagtgc tgagtggaat gctgtcagaa
     2641 aagaatatgg tgagaaaaga acagaccatg aagaaacatg ggaagaaata agaataaaac
     2701 aaaaagacta tgcaaactgc gaacaaaaat ataaagtgct tgttcattta gaagatgaag
     2761 aaggcaatcc actgggaaac tataatgtaa tgctgtgtga atatgatacg gaaaaagagt
     2821 taatagattt attagaagat tattggggat ataaaaagaa tatatataaa gagtattatc
     2881 taaaagttat agattggcca cataatcctg gtccaagtga gtggtggccg ggtagtagtt
     2941 tacatgtatg tgaaatggaa gagagagaat ttcgtgttat caggaaaaaa tataaagaag
     3001 acgatggtcc aatgccttgc ctttatggtt gtccgaattc aagagatata gaagaaaaga
     3061 aagaagtgga aacaaaagta gagattgttt atttctgatt tgtagtgcta aacagacgcc
     3121 gttgtttagg gcaatgtagc cggcttttta gaagtgttta gataagaaac ataaaacaat
     3181 agcctatttt gttccgttgt gctaaatgct ccattttgcc cagtaaagca ggaacttggc
     3241 ctcaaaacct ccactggagc ttttttcgat ctgctaaggc aggagaaggg agtcccaagt
     3301 tcctatggtc aaaattccgc agcacaaagt cgcaaaatag gctatgtttt atgtttcttt
     3361 ttcctatatt ctttgtaaaa tccggaaaaa aatttttgta acgtctgttt ctctaaatct
     3421 tgcaaaaaat cctgcaaatt aatggaacaa aatacaacct tagaagaatc cctctgttag
     3481 aagtcatcga tgcaatccga ccataaatat ctccacagct gcgtttgtca tcaactttac
     3541 aaatcagaaa ttgatagatc agatagcaag aatcttctta gcgttctcca ctcgttcttt
     3601 tgttggtggc tcaattccct ccagtggata ctctaatcca agttctttcc atttataaat
     3661 tcccatcgta tgataaggca ggatttccac cttttccaca ttatcaagag agtgaatgaa
     3721 atcagctaat ctagtcagat attcatcttt atcgcttcgt tccggaacaa ggacatgacg
     3781 aatccaaatt ggttttttga tatcagaaag atagcgaatc atgtcgagaa tattttcgtt
     3841 gcttcgacca gttaaagtct tgtgacattc agtatcaata tgtttgatat ccactaataa
     3901 caggtctgta tatttcatca attcctgaaa tttagaaaag aaaggttcct ctcttgtaaa
     3961 aggtcctcca gcagtatcaa taacagtgtg gattcctttt tcttttgcct ttttaaaaaa
     4021 ctctagcata aaatcaatct gtaaaagagg ctcaccacca cttacggtaa taccgccgct
     4081 atttttccag taagtacgat aacggagtgc ctgatttaac agctcatccg cacttctcca
     4141 caccgcacca ttttcctctg tcatcttcca tgtatctgga ttgtgacaga actgacatcg
     4201 caaaggacaa ccctgtaaaa aaacaataaa acgaactccc ggcccatcca ccgaaccaaa
     4261 actctccgta gaatgaacaa gagccatcgc atttttatta cattctgtca tggcatgttc
     4321 tggcaattac atctaactgc tgttcttttg tcagagagat gaatttaact gcataacctg
     4381 aaacacggat cgtaaagttt gcgtattctt ctttttccgg atgttccata gcatcaatca
     4441 gtttctcaac accaaataca tttacgttta ggtggtgagc accctgtgcg aagtagccgt
     4501 ccataacatt tactaagtta ttgatacgtt cttcttcgga atgaccgagt gcatcagggt
     4561 tgattgtctg tgtattggaa ataccatcta atgcccattc gtaaggaagt tttgctacag
     4621 agtttaagga agctaaaagt ccgttctgct cagcaccata acttgggttt gctcctgggg
     4681 aaagaggttc tccggccttt cttccgtctg gaagggaacc agttgcctta ccatatacta
     4741 cgtttgatgt gatagtaagg atggaagttg taggttcaga atcacggtaa gtatggaatt
     4801 tttcaagctt cttcataaat gtctgaagta accatacagc gatatcatca gcgcggtcat
     4861 catcatttcc gtatttaggg aagtcgccag ttgtctcata atcaacaaca agtccttctt
     4921 cattgcggac tgtttttact ttggcatatt taatcgcact taaggaatct actacatgtg
     4981 agaatcctgc gatacctgta gcgaatgtac gacgtacatt tgtatcgatt aaggccatca
     5041 aagcatattc atagtaatat ttgtcatgca tgtactggat gagatttaat gtatttacat
     5101 ataaacctgc taaccagtcc atcatgatat catatttgtg cattacttca tcatagtcaa
     5161 gatattcgga agtaatcggt ttatattccg gtccaacctg ctgtccggtc ttttcatcca
     5221 caccaccatt gatagcgtaa aggagacatt ttgcaaggtt agcgcgagca ccgaagaact
     5281 gcatttcctt accagtctgt gtagcagaaa cacagcagca gatggagtaa tcatcgcccc
     5341 atactggacg cattacatca tcattttcat actggattga acttgttgta atagaaatat
     5401 gagctgcgta atctctaaat gcttttggaa gacgctcaga gtaaagtact gtgaggtttg
     5461 gctctgggga tggtcccata ttttctaatg tgtggaggaa acggtagtca gtttttgtaa
     5521 ccatggaacg accgtcctgt ccaagtccgc caacttcaag tgttgcccat acagggtcac
     5581 cggagaagag ctggttgtaa gctgggatac gtccgaactt aaccatacgg aacttcatag
     5641 tcatatggtc gattaattcc tgcgcttctt tctctgtaag gattccctta tccatatcac
     5701 gtttgatata aatgtcaagg aaagtagcga tacgtccgat actcatagca gcaccgttct
     5761 gtgtcttgat tgctgcaagg tatccgaaat acgtccattg aacagcttcc tttgcgttag
     5821 aagcaggttc ggaaatatca tagccatagc tttcagccat aactttcata tcttttaatg
     5881 ctttaatctg catggagatt tcttctctta aacggataac gtcatctgtc attgttccgt
     5941 caccgcagtt tgcgaaatct ttttgcttgt cagcaactaa caggtcaata ccgtaaagag
     6001 ctacacgacg gtagtcacca acgatacgtc cacgtccgta agtatctgga agaccagtaa
     6061 tgatcttatt gtgacgagct ttcttcattt caggagtata agaatcaaat actgcctggt
     6121 tatgtgtctt atgataatct gtaaaaatct tgtggaattc tggatttggt gtgtaaccat
     6181 aagttgtaca agcttcttca gcagtacgga taccaccaaa tggcataaat gcacgtttta
     6241 aaggtttatc tgtctgaaga cctacgatct gttctttctc ttttaaagac tcagaaatgt
     6301 aacccggttt atgggaagta aggcttgata cgatatcagt atccatatca agaacaccac
     6361 ccttttcacg ctctgctttc tgcagcttct gcagttcgcc ccacagtgtg tcagtagctt
     6421 cggtaggacc ttcgaggaaa gaagcatctc catcatatgg agtgtagtta tcctggatga
     6481 actctcttaa attaatttct tctctccaga ttcgtccatt aaatccctgc cattgttctt
     6541 tttgtaacat cttaattgac ctccctgttt gttaaaaaaa tccttaagtc aaggttttga
     6601 aatatgtttg ctaaaaaatc aacaaaattg ttcagatgat ttactatgat tagttttaac
     6661 ttatttaagt tttaatcatc taacaacatt attaacatga tttttggcaa tcgtcaaaca
     6721 attttttgat attatgtgtt cttttttttg aacatataca caaaaatgct tgtagagcct
     6781 ttaaaataaa gggttttaga acgttgataa aatattatca aatcgtacaa atgtactgat
     6841 ataagtacaa taatgacaag catacaacta taattttatt tttcgctctt tagagcatgt
     6901 ttggaaaaac atcttcttta gcacatgact taataacgat ttaaagttac ttcttatcag
     6961 ggatattgga tagaatatta tcttatcaga aataaaaaag ccagcatcca gacaagaatt
     7021 gcccagacgg tcggctataa gagtcttata cttcatgtgg ttcattccac ttacttccgt
     7081 cagtcgtata agtatcctaa ctataaacaa aatagaacaa aatgtcaagg ggttactatt
     7141 taattcatta agagaactac agacgaagcc attgcctcca ataaggcata tttgtgattt
     7201 tagtcgcggc gcgttgaatg ttcgctccat gcgctcgcat ccaacacttg ctccgagacc
     7261 acaaatatgc cttattggag gcaatggctt ctgtgctatg gctggagaag atgaattaaa
     7321 cagagggagg ggatttgggg acagaaactg atgtcttctg cttactcaag aatgactggg
     7381 aacaggggga tgatttagct tttttaatgg ttatctcagc aatatttcaa taatttcaga
     7441 ataaaaagca gactgttgtt atcaatatca attcattacc tcttctattt aaatacaaaa
     7501 catctattca caatatttta agagcaagca gaaaac
//
