GCA_959598445.1 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaKIIOHMHI_7 (original name was: gnl|Prokka|KIIOHMHI_7)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS	1	31,351	dipeptide aldehydes	NRP	29%

gnlProkkaKIIOHMHI_13 (original name was: gnl|Prokka|KIIOHMHI_13)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	ranthipeptide	16,450	38,038

gnlProkkaKIIOHMHI_14 (original name was: gnl|Prokka|KIIOHMHI_14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	lanthipeptide-class-ii	22,529	37,433

Compact view

gnlProkkaKIIOHMHI_7 - Region 1 - NRPS

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Location: 1 - 31,351 nt. (total: 31,351 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 31351:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg7_3

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaKIIOHMHI_13 - Region 1 - ranthipeptide

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Location: 16,450 - 38,038 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaKIIOHMHI_14 - Region 1 - lanthipeptide-class-ii

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Location: 22,529 - 37,433 nt. (total: 14,905 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg14_28

ctg14_27

Leader:

MTEEFIGCIEEELKEIELFYEENGVEVEAYTLHNG

Core:

SFFSICC

Core with dehydrations:

DhaFFDhaICysCys

antiSMASH-DB 4.0 matches for ctg14_27

100.0% NZ_JADPAT010000014.1and 2 others:

NZ_JAQDKC010000001.1 Lanthipeptides: PGQ53_RS00010_lanthipeptideNZ_QUIY01000001.1 Lanthipeptides: allorf_008439_008567_lanthipeptide

Lanthipeptides: allorf_07424_07552_lanthipeptide

1

SFFSICC

7

SFFSICC

1

SFFSICC

7

allorf_24026_24139

Leader:

MTCCLMVMNIRKALSAG

Core:

KIHPTERASVFCPYFLVSGL

Core with dehydrations:

KIHPDhbERADhaVFCysPYFLVDhaGL

antiSMASH-DB 4.0 matches for allorf_24026_24139

100.0% NZ_JADMYT010000009.1and 3 others:

NZ_JADNMI010000013.1 Lanthipeptides: allorf_010454_010567_lanthipeptideNZ_QUEF01000006.1 Lanthipeptides: allorf_116827_116940_lanthipeptideNZ_QUIT01000007.1 Lanthipeptides: allorf_004751_004864_lanthipeptide

Lanthipeptides: allorf_115889_116002_lanthipeptide

1

KIHPTERASVFCPYFLVSGL

20

KIHPTERASVFCPYFLVSGL

1

KIHPTERASVFCPYFLVSGL

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg14_27 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 769.3 Da

Molecular weight: 769.9 Da

Number of bridges: 2

allorf_24026_24139 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 2209.2 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2