LOCUS       c00081_gnlPro..        15149 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MJBJNFAH_81.
ACCESSION   c00081_gnlPro..
VERSION     c00081_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-08 00:27:31
            Original ID  :: gnl|Prokka|MJBJNFAH_81
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15149
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15149
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2710:7570]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2711..7570
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..15149
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15149
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(342..1481)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 53.7; E-value:
                     4.4e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg81_1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-17, bitscore:
                     53.7, seeds: 145, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding_C (E-value: 1.6e-09, bitscore:
                     28.8, seeds: 272, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFDRQQQRLLLEGKEYTFEDISQLIAGGAEAHSSAYWNLYLFLN
                     EWFNDSPVITVHTSGSTGIPKELMVRKDQMMQSARLTCEFLNLKKGESALLCMNLRYIG
                     AMMVVVRSLVVGLNLIVRPASGHPLADIKEPLRFVAMVPLQVYNTLQVPEEKERLKQTD
                     ILIIGGGAVDEALETEIKYLPTAVYSTYGMTETLSHIALRRLNGASASEHYYPFPSVKL
                     SLSVENTLVIDAPLVCDEILQTNDIARIYPDGSFMILGRKDNVINSGGIKIQAEEMEKL
                     LRPFIPASFVITSVPDQRLGQAVTLLIVGQLDTRELENKLQTTLEPYYRPKHIFITESI
                     PQTENGKIDRVGCRTLAASYLSHSQQFPSIELHDDAKCS"
     CDS             complement(1513..2538)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg81_2"
                     /sec_met_domain="MR_MLE_C (E-value: 7.8e-30, bitscore:
                     94.0, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILKRFCRQVEKEQGMWDKDVLCQYPSILFGLETAIWHFF
                     AGSWALSDTAFSRGEVGIQINGLIWMGDFDHMLSQIEKKMEAGFRCVKLKIGAIDFEKE
                     LALLRHIRTHFSSKEIELRVDANGAFSPGDAMEKLKRLSEFDLHSIEQPIRAGQWEEMA
                     RLTSESPLPIALDEELIGCNTLDEKKKLLATIRPQYIIIKPSLHGGICGGDEWIMEAEK
                     QHIGWWITSALESNIGLNAIAHWCATFNNPLPQGLGTGMLFTDNIEVPLEIRKDCLWFC
                     K"
     CDS             complement(2711..3535)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_3"
                     /sec_met_domain="ECH_1 (E-value: 1.4e-82, bitscore: 266.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             complement(3539..5206)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-07, bitscore:
                     21.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVIISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINVPISEPLFQFTVDSLPEVRVITRYQGLNVYDRDYNDLVDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVGQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKIIPEPEFAYSEMSAVGTLLKALPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTNGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNVNVRSNLRV
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             complement(5226..6341)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg81_5"
                     /sec_met_domain="Chorismate_bind (E-value: 2e-57, bitscore:
                     184.7, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRVPGEKVPRLLTQAEGAVRL
                     IYDLKELNGQRGFVIAPFQVSESCPVVLIQPDQWGQPLPMDDDTEEDREIALRLQGQES
                     FLTSSTEEYAACFHTFINALRDRTFDKLVLSRHLTVDKVADFSPSSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLDIHATENGPYPAYAGALSHLKTDFRFSLKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYQFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIEDKQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIAVPPLKS"
     CDS             complement(6338..7570)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_6"
                     /sec_met_domain="HBT (E-value: 2.3e-18, bitscore: 56.5,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNG
                     FALFTYHDDTIITDSPENEHIQNEARLNNLQIIKEEEFSAAVDFAPCKCMLVSDDEEAL
                     VGLEDHWKRRLNGALDVFRSEPYFLEVVPCAIDKANSLGALLEVLDVKREEVIAVGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDGVAVAVEKAIIAEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALAETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             complement(7655..8017)
                     /locus_tag="ctg81_7"
                     /transl_table=11
                     /translation="MIQVRIKRVYEDFSETDGYRVLVDKLWPRGMKKEWLKYDYWAKDI
                     TPSSTLRRWFHEDIPGHWEDFVIQYQKELDASPSMADFLTLIKPHPVITLLYASKEPVY
                     NHARILRDYLEMRLKE"
     CDS             complement(8083..9840)
                     /locus_tag="ctg81_8"
                     /transl_table=11
                     /translation="MRFFVCILVLLFVHNQFSRADKTLTDDSLYTEKYIRDIYIPEPRR
                     ALQLLDEAENRKTIPLRVVNELRSLSYSNMYMNKLAFMYARKAYLLDSIYQKDPKHMLK
                     MTVHLAEFSAMMSKYNESMRYALDGIRQAQELGNREAKARLFFCMGENNWRLSFKDKAY
                     DYFNRTIELLRGSKEMREMMLLSYYYGAEMSFLMNDSRIDEALKVGFEREKQIKRLKEV
                     PQISEDYVDGQYSYLYAKLAYIYCMEKKYEKAEQYYQKYLSKKESHTPDGKMYSVPYLA
                     LSGQYEKVIDNCRGFKELMRTQQDTLNEQYLTVLRQEVKAYLGMHKYKEAAEIRETILT
                     ITDSINTRDRNNAALELNVMYGASEKEEYIAEQAFQLKIRNITLCFLACIVVLTLFIVW
                     RLWHFNHIVEYKNRILARLINEKFANRKDGNQLSEVYEQLAVVSEIEPERISPEELEEL
                     ANDTDKESGEEEENKKIFQELNDIVIQKQLYLSSELSREDLAQIVHLNNARFARMIREC
                     TGTNFNGYINELRINYAIELMKKHPNYTIRAIADEAGFNSTPILYNLFKKKTGMTPYEF
                     KKAQDSLQN"
     CDS             complement(9995..10762)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acetyltransf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg81_9"
                     /sec_met_domain="Acetyltransf_1 (E-value: 1e-08, bitscore:
                     25.5, seeds: 223, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METNYITLTKENIADEHICCAFSDKKCKDSYELKKAWLKKEFDNG
                     YVFRRIDERAKVFIEYGPAEKAWVPVSAPNYLMINCFWVSGKYKGCGHGKALLQSAIDD
                     AKSQGKDGLVTVVGTSKFHFMSDTKWLLRQGFQTIEKLPYGFSLLALKINPAAPDPSFN
                     DSVLTGECPDRDGIVVYYTHRCPFTEFHVRGSLVNAAKSKDLPLKIIELETMEQAQNAP
                     TPATIFTLFYNGKFVTTDLSACIEARLGKALGL"
     CDS             complement(10815..11750)
                     /locus_tag="ctg81_10"
                     /transl_table=11
                     /translation="MAQKGELHIRNKHNGQYDFLLLIENYPPLKRFVSLNPLGVQTINF
                     FNPQAVKALNKALLISYYGIRYWDIPKQYLCPPIPGRADYIHYIADLIQPDRVANDLQT
                     EEEDANEQKTKCRCLDVGVGANCIYPIIGHTEYGWTFVGSDIDPVSIENARKIVTCNPV
                     LAHKIDLRLQKDSRKIFDGIIMPGEYFDVTICNPPFHSSKEEAEDGTLRKLSSLKGTKV
                     KKVQLNFGGSANELWCEGGEIRFILNMISESQKYQKNCGWFTSLVSKEKNLEKLCAKLK
                     SVNASEYKIIRMQQGTKSSRILAWRFSNNS"
     CDS             11921..12136
                     /locus_tag="ctg81_11"
                     /transl_table=11
                     /translation="MRGSEKDTLLAPTYHWTLFRARYGEACLINPIYTQWVNMLEKSQK
                     EISFWNNLLPKCEINNLKTKEEIARI"
     CDS             complement(12049..12348)
                     /locus_tag="ctg81_12"
                     /transl_table=11
                     /translation="MRRNNNIKYTYKQIGGITFPILISLLMEMMIGVTDTAFLGRVGEI
                     ELGASAISGVFYLIIFMVAFGFSVGAQILAISSFVFKLLISHFGNKLFQNDISF"
     CDS             complement(12345..13046)
                     /locus_tag="ctg81_13"
                     /transl_table=11
                     /translation="MKYLNQEIENELRNQGAELIRFVDISHLSETQNRQFPHAIVFALP
                     LTAGYIKEVCDTPDYVQARIDDNYNFDDDEYSITENRTHKLADNMAAYIAEKGYNAFSQ
                     SDENLIAEGKFDAAHKESLLPNKTIALLAGLGWIGKNNLLITPEYGAAQCLGTVLTDAP
                     LETFLCETLHPHCGKCTACVNICERKVLKGKVWSTSVSRDEIVDVYGCSTCLKCLVHCP
                     YTRRYCKQSTK"
     CDS             complement(13043..14170)
                     /locus_tag="ctg81_14"
                     /transl_table=11
                     /translation="MNINTETKYCQTCGIPLDIDYASLEEGQNEEYCDYCLKNGVKSYD
                     FSMDYLIYLWGLFPEEYYREVGISYSSSELREIMSKRLPEIKRWKQKINTAHVQYELII
                     KVQEYINCHLFDDLDSDRLSQVAGISKFHFRRLFKAICGDSLGNYIFRLRLEYIAFKLI
                     STDTSVPELLSQINYQNKHTLSRAFKSYFNCSIPEFRKRHSNARPEGKNPIQVELSIKK
                     VHNMRIAYLKLERTNNISHSFSLLWKQLLQFSENYELPSKGCKYVSLTLDYPLITPEEH
                     SRFMVGVTLPQSFETPKGFGMYEISSGEYAIFRFKGLYHELNRVYRHIYLDWLPTSDYT
                     LREPFTFETYINTPEKTPASELITAIYIPVTRKET"
     CDS             complement(14713..14865)
                     /locus_tag="ctg81_15"
                     /transl_table=11
                     /translation="MNLYLYCFFRIFAKVSKSVTGRKLQHICGELCGVIGIEDLCKMLI
                     MSKEP"
ORIGIN
        1 aatttgtcaa tgacgggcaa ctggctatgt tctgattata tcgaactcac gttaacttta
       61 ctattaatga ttcaagcgta aaatagcagt agattgttat acaactgcta tgagaaataa
      121 caagaatgag tatgagaaat gcctgttttt agggacagag cacaggaaaa atatgagaaa
      181 tgccaacttt taaaagtatt tctcatattc tattacatta tgtttcaaca ggttgactta
      241 tattcatgag aatatgagaa atagaattgc caaatcactc tgggggggag gaggctccct
      301 aagttcctca atttacaagt tcactagttt taccgggaac ctcaactaca ttttgcatca
      361 tcatgtagtt ctatggatgg gaactgctgc gaatgggaaa ggtatgatgc tgccaaagtg
      421 cggcagccga cccggtctat tttgccgttt tccgtctgtg gaatagattc tgtaataaaa
      481 atatgtttgg gacgatagta tggttccaat gttgtctgca acttattttc tagctctctc
      541 gtatccaatt gtcccactat caataaagtg acagcctgcc ctaagcgctg atccggaact
      601 gaagtaatga caaaggacgc aggaataaac gggcgaagta acttctccat ctcttctgct
      661 tgaatcttaa ttcctccact gttaatcaca ttatctttcc ggcctagaat cataaaactt
      721 ccatcgggat agatacgtgc aatgtcattg gtttgcaaaa tctcatcaca gacaaggggg
      781 gcatcaatca ccaatgtgtt ctctacggat aaagacagtt ttactgaagg aaatggataa
      841 taatgttctg aagccgatgc tccattcaga cgacgaaggg caatatgcga caaggtttca
      901 gtcatgccat aagtggaata aacggcagtc ggaagatatt ttatctccgt ttccagagcc
      961 tcatcaacgg ctccgcctcc tataatcaga atatccgttt gctttaatcg ttctttctct
     1021 tcgggaactt gcaacgtatt ataaacttgc aggggaacca ttgccacaaa tctcaaaggc
     1081 tcttttatat ccgctaaagg atgaccggaa gcaggacgta caatcaaatt caaacctaca
     1141 accaatgaac gtacgaccac catcattgca ccgatataac gaagattcat gcataataat
     1201 gccgactcac ctttcttcaa attcagaaat tcacaagtca gacgcgcact ttgcatcatt
     1261 tgatccttgc gtaccatcag ttctttaggt atacccgtgg agcccgatgt atgaactgta
     1321 ataacaggag aatcattaaa ccattcgttc aggaaaagat ataaattcca ataagcagaa
     1381 gaatgagctt ctgctcctcc tgctatcagt tggcttatat cttcaaaagt gtattctttt
     1441 ccttccagta gcaagcgctg ttgttgtcga tcaaatatca tcagaatatc ctttaaatac
     1501 cactaaatac catcatttac aaaaccacag gcaatctttt ctgatttcaa gaggcacttc
     1561 tatattgtca gtaaagagca tacccgttcc caatccttgt ggcaacggat tattgaatgt
     1621 tgcacaccaa tgggcgatag cgttcaaacc aatgttggac tctaaagcgg aagtgatcca
     1681 ccagccgata tgttgctttt ccgcttccat aatccattca tctcctccgc atattcctcc
     1741 gtggagagat ggtttaatga tgatatattg cggacggatg gttgccagca gtttcttttt
     1801 ttcatccagc gtgttgcatc ctatcagctc ttcatccaat gctatcggca acggagattc
     1861 agaagtgagc cgggccattt cctcccattg tccggcacgg atcggctgtt cgatagaatg
     1921 taaatcaaat tccgacagac gtttcagttt ttccattgca tcacccggag agaaagcacc
     1981 gtttgcatct acacgcaact ctatttcttt agaagagaaa tgggtacgaa tatggcgtaa
     2041 caacgccaat tctttttcaa aatcgatagc accaatcttt agtttgacac aacggaatcc
     2101 ggcttccatc tttttctcga tttgggataa catgtgatca aaatctccca tccaaatcaa
     2161 accattaatt tgaatcccta cctctccacg agaaaaagcc gtatcggaca aagcccaact
     2221 ccctgcaaag aaatgccaga tagccgtttc caagccaaat aagatggaag gatactggca
     2281 taaaacatcc ttatcccaca ttccttgttc tttctctact tgccgacaaa atctttttaa
     2341 tatatcttcg taatcaggaa gatcgtcaca acttaaagcg ggcaaaggtg cacactcccc
     2401 tattcccact ctgccaggga agtcaggaga ggtaaaatga agataccaaa ctttacgcgt
     2461 agtatatgtt cctctggaag tcccggcagg ttgcttgaaa tggagcaatc ggggaataat
     2521 ttctatttta caattcatcg tgaataattt ttcaattcac cacagaataa cacagagcct
     2581 cacgaagttt taataagtta tatctcttgc agttctccta ccattctctc ctccacagtc
     2641 ttgctttgca gaaaatcggg cttaaacgca ggaaaagcaa actctgtggt actctgtggc
     2701 aaagccgatt ttaagggaat ttcggatatt gtttgaagtt cggtttacgt ttctccagaa
     2761 acgcattctt gccttcctgt gcttcatcag tcaggtaata gagcaacgtt gcgtcacctg
     2821 ccaattcctg aatacctgcc tgaccatcca gttcggcatt caatcctgct ttaatcatgc
     2881 gcaaagccaa cggactaagc aacatcattt cttccgccca ctgtacatat tcgtcctcca
     2941 actgctccaa aggaactact ttattcacta atcccatgtc caatgcttct tgtgcattat
     3001 acttccggca gaggaaccag atttcgcgcg ctttcttctg ccccactaca cgtgccagat
     3061 aagacgcacc gaatccggca tcaaagctac cgacacgagg acccgtctga ccgaaaatag
     3121 cattttccga cgcaatggac aaatcacata ccacatgaag cacatgtccg ccaccaatag
     3181 caaaaccatt cacggcagca atcaccggtt tcgggatgct gcgaatctgc ttttgcacat
     3241 ccaatacact aagacgaggc acaccgtctt ttccaatata tccgccacgt cctttcacat
     3301 tctggtcgcc acccgaacaa aatgccttat caccggctcc ggtaatcaca atcacatcaa
     3361 tatccgcttc ttcacggcag atgcgcaatg cgtcactcat ttcagcagta gttgtcggag
     3421 taaacgcatt ccgatagcgt tcacggttga tcgtgatacg ggcaattcca ttataataat
     3481 caaaaagaat atcgtcgtat tctctaatgg ttgtccactc tctttgtgtt gacataatct
     3541 atttttgttt taattgatga taataattct tcaacatgcg cgcgtcctta tttttgttgg
     3601 taaacacctc caagagcaca ggctgctcca tagcttccgg ttgagtgaaa gttttcatcg
     3661 cttcagccag ttcttcgtca ttttgcgctt gcagataaag aaacccacgc tcttctgccc
     3721 aacctttggc cgatgtttta tgaactgccg caataaattt atgtgaggta cccgacatat
     3781 ccaatcccgg caaggtatgg aaaatctctc ctcctccatt atttaacaga agaacacgca
     3841 gattggagcg aacattgacg ttccacaacg cattcatatc atagaagaag ctcaaatctc
     3901 cgatagctat aaagttcagt ttgtcggaag ctgccgcata accgacagcc gtagacagcg
     3961 agccttcaat gccgtttgtt ccccggttgc aacaaacttc aatcgtagaa ggaatggagt
     4021 ataattgcgc atagcgcacc accgaactgt tagccagatg caacgcacat gactccggca
     4081 aggctttcaa caacgtaccg acagcagaca tttccgaata agcaaactcc ggttcaggaa
     4141 tgatcttgca atagttttcc cacacacggg gatattcagg agtccgattg tccagcaaac
     4201 tggctatctt ctccagaaac tcgaacgggt ccatttcgat caccgtagtt aatgagccat
     4261 acaaatcgac cacctcacca tccggcgata cgtgccaatg ttctttaggc ggatgctgac
     4321 ggagaaactt cttcagccgt ttggaaacga catgtcctcc ataagtaatc aacaactccg
     4381 gaaccatctg gccgaccttc tcttcgggca tggcataaag cgccgcatca aagttcttta
     4441 ccggaatacc gggaactgtc tggttgccga tatgttccgt caaccaggca aaatgtttat
     4501 ataataactt gatatatctt ttttcaaaca aatagataag attcatctga ccgatgataa
     4561 tcattcgctt ctggtattta ttcatccgat cgaccaaatc gttataatca cgatcgtaga
     4621 cgttcaaccc ctgataacgg gtaatgacac gcacttcagg aagcgaatcg accgtaaact
     4681 ggaacaacgg ttcagaaata gggacattga tatgtaccgg tccttttcca tgatggttcg
     4741 tttccagcaa agcttcattc accagacgat tgcaatacca ttcatcttct tccgtatgaa
     4801 tctccggaag gtttaccgac ttcttgacca gcgtctgaaa tacgcctggt tgtggtacag
     4861 tctgtccgtc catttgtccg atccaggcag cgggacgatc cgcagaaata atgaccagag
     4921 gaacattttg ataaaatgct tctgccacag ccggatgaag gtttaataat gcagtaccgg
     4981 aagtgcaaca gacagctgcg ggcttaccac cattcaacgc aagaccgatg gcaaaatagc
     5041 cggcactccg ttcatccgtc accggatagc aggtaaaatt cggatggtta gacaaagtat
     5101 gtactatggg cgtattccga ctacccggac acaacacaac cttagtaatc ccatgcgcct
     5161 cgagcaacgc aaccaactgg agtatgttct tcttgtctgt atacatgatg ataattatta
     5221 gtagtttatg attttaaagg aggcactgca atcagccgtt tgatggtttg caactttttc
     5281 tccgtttcca gccattcgtc attcaattcc gaagaggcca ataatccacc gccggcataa
     5341 agagtaagtt gtttatcttc gatatgcata cagcgtaagt ttacatagat gtctgttcgc
     5401 ccttccggat cgagccatcc gataaagccg gagtaatagc ggcggtcgta accttcattc
     5461 tgcaaaataa actgataggc ttcttccttc ggcaagccgc atacggcagg tgtcggatgc
     5521 aaaactttca gaaggtctcc caaaccttta ttatctttca aagaaaaacg gaaatcagtt
     5581 tttaaatgtg acaaagcacc ggcataagca ggatatggcc cgttttccgt agcatgaata
     5641 tccagtgaaa gaagctgccg acggatataa gaagcaacat aatcttgttc tttccggttc
     5701 ttttcatccc atacttgcgg cagtctgcca tcttgtaaag gctgcgttcc ggctagcgca
     5761 accgtatgcc attcgtcttt ctcacctgat aaaataattt cgggagtgct tcccagccag
     5821 atacctgtct gcggagtata acataaatat atatacgaat gaatataacg tttacaagcc
     5881 gcccgaaaga tggacgaagg agagaaatca gctactttat caactgtaag atgacgggaa
     5941 agcactagtt tatcaaacgt cctgtcacgt aacgcattta taaaagtatg aaagcatgcc
     6001 gcatattctt ccgtggaaga agtcagaaat gattcttgcc cttgcaggcg caaggctatc
     6061 tcccggtctt cttccgtatc atcatccatc ggtaaaggct gcccccactg atcgggttgg
     6121 atcaacacaa ccggacatga ctcactcacc tgaaacggag caattacaaa tcctctctgt
     6181 ccgttcagtt ctttcaaatc gtatatcaaa cggaccgctc cttccgcctg cgtcaacaga
     6241 cggggaacct tctctccggg aacacggtaa acagcaaacg gctgcttccg ttgaataaat
     6301 gcatcaatag ttgtcaagtt acttatttct tcgtcggtca tctctttttc atcacactgt
     6361 tgactacccg tatggaagac accagtttgt ttgttgaagt aaacacgtct acattccata
     6421 cgtgggagga acgtccctta tgtacaactg ttcctactgc acgcacggta tctccttcat
     6481 gtgcagaaga tatatggttt ccgctgacct gcattcctac tacaatctcg tccggctggc
     6541 agagaatcat cgaaccgagt ccggcaactg tttctgccag tgcaagagta gctcccccat
     6601 gcaaaatgcc gaaaggctga cgggtacgat ggtctaccgg catcgttgct tccacacggt
     6661 cttcgtcggc gtatgtatat tgaattccca agtttcccat taatgcgtgg cgtgcctgcg
     6721 cattcagttg gtcaagggga atttcggctg cacgcacttc ggctataata gccttctcca
     6781 ctgcaacggc cactccatct tcctcattcg aagcggtcac atagtcggca caggctttca
     6841 ccgagtcttg tgaatgtccc atagctacgc ccagcccggc caattgaatc attgttacgt
     6901 cacacacacc gtcgcccaca gcaatcactt cctcacgctt cacatccagt acctccagca
     6961 aagcacccaa agaattggct ttgtcgatgg cgcagggaac tacttccaga aaatatggct
     7021 ccgaacggaa tacgtccaac gctccgttca atcgtctctt ccagtgatct tccagaccaa
     7081 ccagtgcttc ttcgtcatcg ctcaccagca tacacttaca aggagcaaaa tcaacggcag
     7141 cagaaaattc ttcttctttg attatttgca ggttattcaa ccgggcttca ttttggatat
     7201 gttcattttc aggagaatcc gttatgatcg tatcatcatg ataagtgaat aaggcaaaac
     7261 cgtttttacg ggctttcttt tccagatacg gcaacatttc gggattaatc cgacgttcaa
     7321 acagaatttc cccgttttgt gcattgataa tctgacagcc attataggaa aggataaaac
     7381 ctccatagtt tccaagttca agcatcttgg ccagcggcat caggccataa gtaggtctgc
     7441 cggatgccag cacaatacgt actcccattt gctgaacctt caacagggca gccaatgtac
     7501 gcttactaat ttctttcgca tcattgagga gtgttccgtc tacatcaaga accaataatt
     7561 tatacttcat gattacacga ttttgattta gattacaaag gtaggaaatt gagtgaacga
     7621 cagaaagcgt aaacagataa aaattacttt aatttcactc tttcaaacgc atttccagat
     7681 aatcgcggag gatacgggca tgattataca ccggttcttt ggaagcgtaa agcaatgtta
     7741 tcaccggatg cggcttgata agagtcagaa aatcagccat tgagggagaa gcatctagtt
     7801 ctttctgata ctgtatgaca aagtcttccc agtgtccggg aatatcttcg tgaaaccatc
     7861 tacgcaatgt ggaagacggt gtgatgtctt ttgcccaata atcgtactta agccattctt
     7921 tcttcattcc acgtggccat aatttatcta ccagtacccg gtagccatct gtttctgaaa
     7981 aatcttcgta aacccgcttt atccttacct gtatcatctc tattaatagt taattataaa
     8041 tagataaacc ggaaaatgcg aaaattgttt agcagaatcc actcaattct gtaacgaatc
     8101 ctgtgctttt ttaaattcgt aaggagtcat tcccgtcttt ttcttaaaaa gattgtataa
     8161 aatcggggtg ctattaaatc cggcctcatc tgcaatggca cgtatcgtat agttgggatg
     8221 ctttttcatc agttcaatgg cataattaat gcgcaattca ttgatatatc cattaaaatt
     8281 ggttccggta cattctctta tcatcctcgc aaaacgtgcg ttattcagat gtacgatttg
     8341 tgccagatct tctctcgaca attctgaaga aagatataac tgtttctgaa taacgatatc
     8401 attcagttcc tgaaatatct ttttattctc ttcttcttcc ccactttctt tatctgtatc
     8461 gttagcaagt tcttccagtt cttcgggtga aatacgttcc ggttcaatct ccgacacaac
     8521 agccagttgt tcgtatactt ccgatagctg gttcccatct tttctattag caaatttctc
     8581 gtttataagc ctggcaagta tcctgttctt atattcgact atatgattga aatgccacaa
     8641 acgccaaacg ataaacagag tcaacacaac aatacacgca agaaaacaga gggtaatatt
     8701 tcttattttt aattgaaaag cctgttcggc aatatattct tctttttcag aagcgccgta
     8761 cattacattc aattccaatg cagcattgtt tctatcccgg gtattgatac tatctgttat
     8821 agttaaaatc gtttcacgta tctcggcagc ttctttgtat ttatgcatac ccaaatatgc
     8881 cttcacttcc tgtcgaagga cagtcagata ctgttcattc aaagtatcct gctgggttct
     8941 catcaattcc ttgaaccccc tgcaattatc aataaccttt tcatattgtc cggaaagcgc
     9001 caaataagga accgagtaca tttttccgtc gggagtatga gactctttct ttgataagta
     9061 tttctgataa tattgctccg ccttctcata tttcttctcc atgcaataga tgtaggctaa
     9121 tttggcatat aaataactat attggccatc tacataatct tcagagattt gaggtacttc
     9181 cttaagccgt ttaatctgct tttcacgctc aaaacccact ttcaatgcct catcaattcg
     9241 agaatcattc attaaaaagc tcatttctgc tccatagtaa taagagagca gcatcatttc
     9301 ccgcatctcc tttgagcccc gcaaaagctc aatagtccgg ttaaaataat cgtatgcttt
     9361 atctttaaaa gataatctcc aattattctc ccccatacag aaaaataacc tggctttggc
     9421 ttccctgttc cctaattctt gcgcctgcct gattccatct aatgcataac gcatactttc
     9481 attatatttg ctcatcattg ccgaaaactc agccaaatgt actgtcattt tcagcatgtg
     9541 tttcggatct ttttgatata tggaatcaag caaataagct ttccttgcat acataaatgc
     9601 caatttattc atatacatat tactatatga taaactacgc aattcattta caacccgcaa
     9661 aggaatcgtt ttcctgttct ccgcttcgtc cagtaattgc agagcacgcc taggttcggg
     9721 aatataaata tccctgatat atttctccgt atacagagaa tcatctgtta aagttttatc
     9781 agccctcgaa aattgattat gtacaaataa aagcaccaaa atacatacaa aaaaacgcat
     9841 aagttcttac cgtgttttaa tcattcaaca tccaaagata tgtattttcc ataaaaagtg
     9901 caaggtacat cttaattttg caaaattaaa ataagaaaca ttagctatct tcttatccca
     9961 atcctagttt ctgataaatc ttcatctcac agaatcacaa gcctaaagcc tttcccaacc
    10021 gggcttcaat acacgcgctc aaatcggtcg taacaaactt cccattataa aaaagagtaa
    10081 agattgtggc tggagtaggc gcattttgtg cctgttccat ggtttctaat tctattattt
    10141 ttaatgggag atctttactc ttggctgcat tgaccaaaga accgcgaaca tggaattctg
    10201 taaacggaca gcgatgagta taatacacta ctattccatc ccggtcggga cactcacctg
    10261 tcaatacact gtcgttgaat gaaggatcgg gagcagccgg atttattttc aaagccaaca
    10321 aactgaatcc gtaaggaagt ttttctatag tttggaaacc ttgtcgcagc agccattttg
    10381 tatcactcat aaaatgaaat ttagaagtgc ccactacggt aactaaacca tcttttccct
    10441 gcgatttcgc atcatcaata gcagactgca gcaaagcctt gccatgcccg catcctttat
    10501 atttacccga cacccagaaa caatttatca tcagataatt aggagcactg acaggaaccc
    10561 acgccttttc agcaggacca tattcgatga acactttcgc ccgttcgtca atacgtctga
    10621 aaacataacc attatcaaac tcttttttca gccatgcttt ttttaactca taactatctt
    10681 tgcatttctt atccgaaaag gcacagcata tatgttcgtc cgcaatattc tccttcgtca
    10741 aagtgatgta atttgtttcc atataatatt ttatttaata ggtttaaaaa taacagatca
    10801 ataagcactc attattatga gttattagaa aatcgccaag ctaatattct gctgctttta
    10861 gttccctgct gcattctgat aatcttatac tccgatgcat tcacagactt caatttagca
    10921 catagctttt caagattctt ttcttttgaa accaagctgg taaaccatcc acagttcttc
    10981 tgatatttct gactctcgga gatcatattc agtataaaac ggatttctcc accctcacac
    11041 caaagttcat ttgcactccc gccaaaattt aattgtactt ttttcacctt cgtccccttc
    11101 aggctactca atttgcgcaa ggttccgtct tccgcttctt ccttcgagct atgaaacgga
    11161 gggttacaga tcgtaacatc aaaatactcg ccaggcatga taattccatc aaaaattttc
    11221 cgactgtctt tctgaagtcg caagtcaatc ttatgtgcca ataccgggtt gcaagtcact
    11281 atcttccgtg cattctcaat cgagaccgga tcaatatcac tgcccacaaa tgtccatcca
    11341 tattctgtgt gccctataat cggataaata caatttgcac ctaccccaac atccagacat
    11401 ctgcatttcg ttttctgttc gttcgcatct tcctcctctg tctgcaaatc gttcgctacc
    11461 ctatccggct gaataagatc ggcaatataa tgaatataat ccgctcttcc aggaatgggt
    11521 ggacacaagt attgtttcgg tatatcccag taccgaatgc cataataact gattaataat
    11581 gctttattta aagcttttac tgcttgcgga ttaaaaaagt ttatagtttg tacacccaga
    11641 ggattaagtg atacgaatct cttaagtgga ggataatttt ctatcaacaa cagaaaatcg
    11701 tattgaccat tgtgcttatt tcttatatgt aactcacctt tttgcgccat gtcggcaaag
    11761 atacagagaa atttcctgtc tgcaaatttt ccctaactct tacctcataa catcactaac
    11821 attcggtgtc ttttttcggt tcttctttta tctgccgagt tatagattat gtttcaattt
    11881 tgcaagtctt taattactaa tatttaaact tagacataag atgagggggt ccgaaaagga
    11941 taccctcttg gcaccgactt atcattggac tttgtttaga gcaagatatg gtgaggcttg
    12001 cttgataaat cctatctata cacaatgggt aaatatgtta gaaaaatctc aaaaagaaat
    12061 atcattttgg aacaatttat tgccgaaatg tgaaattaat aacttaaaaa caaaagagga
    12121 aatcgcaaga atctgagctc cgacactgaa accgaaagca accataaata taatcaagta
    12181 gaacaccccc gagatggcag atgctcccaa ctcgatctct ccgacacgac ccagaaaggc
    12241 tgtgtccgta acgcctatca tcatctccat cagcaggctg ataagaatgg gaaatgtgat
    12301 acccccaatt tgtttatagg tatattttat gttgttattt ctcctcattt tgttgattgt
    12361 ttacaatacc gacgagtata cgggcaatga actaaacact ttaagcaggt gctgcatcca
    12421 tatacatcga cgatttcatc tcttgaaaca gaggtgctcc aaaccttacc tttaagaact
    12481 tttctctcgc agatattgac acaagcagtg cactttccgc aatgcggatg gagagtctca
    12541 cacaaaaacg tttccaacgg ggcatcggtt aaaactgtac cgagacactg tgccgcacca
    12601 tattcaggag tgattaacag attgtttttt ccaatccacc ctaatccggc gagcaaggct
    12661 atcgttttat tcggcaaaag agactcttta tgtgctgcat caaattttcc ctcggcaata
    12721 aggttttcat ccgattgcga aaaagcgttg tatccttttt ctgctatata cgctgccatg
    12781 ttatctgcca atttatgagt cctgttttcg gtaatggagt attcgtcgtc atcgaaatta
    12841 taattgtcat ctatccgggc ctgtacgtaa tcgggggtat cacatacttc ttttatatat
    12901 ccggctgtca gcggaagtgc aaatacaatg gcatgaggga attgcctgtt ttgtgtttca
    12961 ctcaagtgtg atatatcaac aaagcgtatc agttctgctc cttgatttct taattcgttt
    13021 tctatctctt gattcaggta tttcatgttt cttttcttgt tacagggata taaatagctg
    13081 ttatcaactc cgaagccgga gttttctcag gtgtattgat atacgtttca aaagtgaaag
    13141 gctctcgcaa cgtataatca cttgtgggca accagtcaag gtagatatgg cgatacaccc
    13201 tatttagctc gtggtataat cctttgaaac gaaagatggc atattcgccc gaagaaattt
    13261 catacatgcc aaagccttta ggggtctcaa atgattgagg caaggttact ccaaccataa
    13321 aacggctgtg ctcctcaggc gtaatcaaag gataatcaag agtcaggctt acatatttgc
    13381 atccttttga tggcagttca tagttttcgg agaattgtaa caactgtttc cataagagag
    13441 aaaaactatg actgatgtta tttgtccttt ccaacttcaa ataggcaatg cgcatgttgt
    13501 gcactttctt tatgctcaac tcaacctgta ttggattctt tccttcgggg cgagcattgg
    13561 aatgtctctt gcgaaattct ggtatagaac aattaaaata actcttgaat gctcttgaaa
    13621 gcgtatgttt attctgataa ttgatttgac taagaagttc aggaacactt gtatctgtcg
    13681 atattaactt aaatgcaatg tactccaatc ttagccggaa gatgtaattt ccaagactat
    13741 caccacagat tgctttaaaa agtctacgaa aatgaaattt agaaatacct gcaacttggg
    13801 atagcctgtc tgaatcgagg tcgtcaaaca agtggcagtt aatgtattcc tgaactttta
    13861 taattaattc gtactgcaca tgggcagtgt tgattttttg cttccatctt ttgatttccg
    13921 gcaatcgttt ggacataatt tcccttaatt ccgatgaaga atagcttatt ccgacctctc
    13981 tataatattc ttcggggaaa agcccccaaa ggtagatgag gtaatccatt gagaagtcat
    14041 acgacttaac tccatttttt aggcagtagt cgcaatattc ctcattctga ccttcctcta
    14101 aactggcata atctatatct aagggtattc cacacgtttg gcaatatttt gtttcagtat
    14161 taatgttcat acacgtctca ttatttaagt gcaaaattac tcaataaatt acggaaaggg
    14221 ttaccctaaa tggctatctt ttactttatc ataatctcct ccaagcaggt aaatcacatt
    14281 tttgtataac acgttcatca actttttgcg gatcaagctt tttaggtata aaagtagtat
    14341 atacttcaag ctgatagcat tgatgcagta ctatatcccg ttgaatacca tcgatagatg
    14401 tttctataaa ttcgaatatg tcctaaatag acactagtgg tttaaaatag ccgttctcta
    14461 tattggatac tatgtattct agtgttcttt tctagaggta ttgcatatct gcctctattt
    14521 ttacattttc ggtgaatggc tccaaatatt caggctgatc cagtattcag actgagccgt
    14581 ctaaatgatg tccggtagag cagacataat gggataaaaa aagtcccaca aatcattgaa
    14641 tttgtgggac ttgactgttc attgcccggt tttgtccgga tagttcagcg gagagacagg
    14701 gaacgattaa ctttaaggtt ccttactcat tatcagcatc ttacataaat cctcaattcc
    14761 tatgacaccg cataattcac cgcatatatg ctgcaacttt cttcctgtta cacttttact
    14821 tacttttgca aatatacgaa aaaagcagta taaatacaaa ttcatcactt gatttaacga
    14881 agataacttc tataattttt ttccaatacc gattgcaatt tggaaagagg ataaaggaac
    14941 ttgccaccta taacagtata agctatcccc ttttcatccc gcaacttttg caaagtcctg
    15001 cttgatatat gtaacatact acatacctgc tcaccagtca tataaacctc attagctaca
    15061 ggaacctgca aattctgaaa ttcaagaatt tccttggttc ttcgtcaaat ttggtggtaa
    15121 tctctttttt atttcgatgg ttttcctct
//
