LOCUS       gnlProkkaBNAFLIDF_228   3744 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BNAFLIDF_228.
ACCESSION   gnlProkkaBNAFLIDF_228
VERSION     gnlProkkaBNAFLIDF_228
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-02 08:51:39
            Original ID  :: gnl|Prokka|BNAFLIDF_228
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3744
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3744
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:2918]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..2918
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..3744
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3744
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             3..2918
                     /NRPS_PKS="Domain: AMP-binding (4-395). E-value: 4e-95.
                     Score: 310.6. Matches aSDomain:
                     nrpspksdomains_ctg228_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (500-567). E-value: 1.8e-21. Score:
                     68.0. Matches aSDomain: nrpspksdomains_ctg228_1_PCP.1"
                     /NRPS_PKS="Domain: NAD_binding_4 (611-849). E-value:
                     2.7e-36. Score: 116.8. Matches aSDomain:
                     nrpspksdomains_ctg228_1_NAD_binding_4.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     319.8; E-value: 4.4e-97)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_1"
                     /sec_met_domain="AMP-binding (E-value: 3.5e-105, bitscore:
                     339.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.6e-12, bitscore:
                     35.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.4e-09,
                     bitscore: 25.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.9e-15, bitscore:
                     44.3, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.4e-37, bitscore:
                     116.8, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDLFKEMAARYPDRPAVRDDSGDFTYRELDRMSDYIAQKLTENG
                     FGPEQAAGILCGRTKEYTVAYVGVMKAGGAYVPLDPEYPQSRIEYMLKDSGARNLLVID
                     QYQNLVEFYDGNVISLDSVADEAEDFELSAELISPKPENLAYMIYTSGSTGKPKGVMIE
                     HRNLLNLIEYITLSRNTSPDDIVAEFASFCFDASVIDLFAPLTAGAVLYILPESIRKDA
                     IAISRYIKEKEITTVTFPTQMGELVTELLEDAPALKFVTLGGEKFKHYRNRTYQMINGY
                     GPTENTVSSTEFLVDRQYDNIPIGKSQRNVRSYIVDENLNRLPVGASGELCHAGRQIAR
                     GYHNLPEKTASVFVENPFAVCEQESRLYRTGDMVRMKGDGNIEYIGRIDSQVKIRGYRV
                     ELGEIEGALLKHELVKNAAVTVIEKGGNKYITAYYTGEAIPEDELKTFLEPLIPDYMMP
                     SFFVSIEEMPVTPGGKIDKKALPMPEVTTNTASYVEPVTAAQRALCEIFEKALGIERVG
                     IEDNFFELGGSSLTASKVAVMCLSKNISLVYADIFKYPTVRELAALVDDDGVTEAAQSK
                     NEFSDYNYNRIQNVISANTEENADRITKEKLGDIMVTGATGFLGIHVLKAFLDNYDGKV
                     YCLVRKGKYESPEKRMMNMLMYYFDDPYKELFESRIICVDGDITSKEQVTGFSEYKFST
                     IINCAACVKHFAADDVLERINVQGVENLIDFCKNNGRRLIQISTVSVAGEGSDGVPPMS
                     RVFCENDLYIGQNITNEYIRTKFLAERAVLEAVSEGLDGKVVRVGNLMSRNSDGEFQIN
                     FITNGFLRSLRGYAAVGKFPMGGMHEVAEFSPIDSTALAVLRLAQTDRRFTVFHACNSH
                     HIYMADLIYAMRSYGFRIDIVRDEEFEAAVKEFAKTGQDSDAVSGLIAYTSHNENEIYT
                     IDYSNRFTAQVLYRLDYKWPVTDDRYLENAIAALDRLTFFD"
     aSDomain        15..1187
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg228_1_AMP-binding.1"
                     /evalue="4.00E-95"
                     /label="ctg228_1_AMP-binding.1"
                     /locus_tag="ctg228_1"
                     /protein_end="395"
                     /protein_start="4"
                     /score="310.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FKEMAARYPDRPAVRDDSGDFTYRELDRMSDYIAQKLTENGFGPE
                     QAAGILCGRTKEYTVAYVGVMKAGGAYVPLDPEYPQSRIEYMLKDSGARNLLVIDQYQN
                     LVEFYDGNVISLDSVADEAEDFELSAELISPKPENLAYMIYTSGSTGKPKGVMIEHRNL
                     LNLIEYITLSRNTSPDDIVAEFASFCFDASVIDLFAPLTAGAVLYILPESIRKDAIAIS
                     RYIKEKEITTVTFPTQMGELVTELLEDAPALKFVTLGGEKFKHYRNRTYQMINGYGPTE
                     NTVSSTEFLVDRQYDNIPIGKSQRNVRSYIVDENLNRLPVGASGELCHAGRQIARGYHN
                     LPEKTASVFVENPFAVCEQESRLYRTGDMVRMKGDGNIEYIGRIDSQVKIR"
     aSModule        15..2549
                     /complete
                     /domains="nrpspksdomains_ctg228_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg228_1_PCP.1"
                     /domains="nrpspksdomains_ctg228_1_NAD_binding_4.1"
                     /locus_tags="ctg228_1"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       204..242
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg228_1_0001"
                     /evalue="9.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg228_1"
                     /protein_end="80"
                     /protein_start="67"
                     /score="18.8"
                     /tool="antismash"
                     /translation="GVMKAGGAYVPLD"
     CDS_motif       435..494
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg228_1_0002"
                     /evalue="2.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg228_1"
                     /protein_end="164"
                     /protein_start="144"
                     /score="35.4"
                     /tool="antismash"
                     /translation="AYMIYTSGSTGKPKGVMIEH"
     CDS_motif       972..1061
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg228_1_0003"
                     /evalue="5.60E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg228_1"
                     /protein_end="353"
                     /protein_start="323"
                     /score="41.0"
                     /tool="antismash"
                     /translation="PVGASGELCHAGRQIARGYHNLPEKTASVF"
     CDS_motif       1152..1217
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg228_1_0004"
                     /evalue="2.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg228_1"
                     /protein_end="405"
                     /protein_start="383"
                     /score="38.4"
                     /tool="antismash"
                     /translation="YIGRIDSQVKIRGYRVELGEIE"
     aSDomain        1503..1703
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg228_1_PCP.1"
                     /evalue="1.80E-21"
                     /label="ctg228_1_PCP.1"
                     /locus_tag="ctg228_1"
                     /protein_end="567"
                     /protein_start="500"
                     /score="68.0"
                     /tool="antismash"
                     /translation="RALCEIFEKALGIERVGIEDNFFELGGSSLTASKVAVMCLSKNIS
                     LVYADIFKYPTVRELAALVDDD"
     aSDomain        1836..2549
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg228_1_NAD_binding_4.1"
                     /evalue="2.70E-36"
                     /label="ctg228_1_NAD_binding_4.1"
                     /locus_tag="ctg228_1"
                     /protein_end="849"
                     /protein_start="611"
                     /score="116.8"
                     /tool="antismash"
                     /translation="VTGATGFLGIHVLKAFLDNYDGKVYCLVRKGKYESPEKRMMNMLM
                     YYFDDPYKELFESRIICVDGDITSKEQVTGFSEYKFSTIINCAACVKHFAADDVLERIN
                     VQGVENLIDFCKNNGRRLIQISTVSVAGEGSDGVPPMSRVFCENDLYIGQNITNEYIRT
                     KFLAERAVLEAVSEGLDGKVVRVGNLMSRNSDGEFQINFITNGFLRSLRGYAAVGKFPM
                     GGMHEVAEFSPIDSTA"
     CDS             2938..3240
                     /locus_tag="ctg228_2"
                     /transl_table=11
                     /translation="MEIITKTEGNKATMEISGWLDTQTAPQLGEALSGLEDSITSLVFD
                     FTNLEYISSAGLRQVIVAYKKMAQKDGFKIINVSDEVYDVFSLTGFDSKIDIQKK"
     CDS             3252..3743
                     /NRPS_PKS="Domain: ACPS (98-158). E-value: 2.9e-13. Score:
                     41.7. Matches aSDomain: nrpspksdomains_ctg228_3_ACPS.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012:4'-phosphopantetheinyl transferase (Score: 124.4;
                     E-value: 6.9e-38)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg228_3"
                     /transl_table=11
                     /translation="MQKLYRININHFEDPLKNERLLDLVGIRRREKVIRYRMPDDRKRS
                     LGAGIIIRKILDENGLTESCLRYSDNEKPVVDGLFFNVSHAGDYVVGVLSDCEVGCDIE
                     KNANAPLEVAEHYFYHSELAYIKAAKNKDKAFFTLWTLKESYMKMTGRGMSLPWDSFEV
                     V"
     aSDomain        3546..3725
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg228_3_ACPS.1"
                     /evalue="2.90E-13"
                     /label="ctg228_3_ACPS.1"
                     /locus_tag="ctg228_3"
                     /protein_end="158"
                     /protein_start="98"
                     /score="41.7"
                     /tool="antismash"
                     /translation="VGCDIEKNANAPLEVAEHYFYHSELAYIKAAKNKDKAFFTLWTLK
                     ESYMKMTGRGMSLPW"
ORIGIN
        1 ccttcataga tctgtttaag gagatggctg ccagatatcc ggacagaccg gcagtgcggg
       61 atgactccgg agattttact tacagagagt tggacagaat gtctgattat attgctcaga
      121 agcttacgga aaacggattt ggcccggaac aggcagcagg tatcctgtgc ggcaggacaa
      181 aggaatatac agtagcctat gtgggtgtta tgaaggccgg cggagcctat gttcctctgg
      241 atccggagta tccacagagc agaattgaat atatgctgaa ggattcagga gcaagaaatc
      301 tccttgtgat cgatcagtat cagaatcttg tggaatttta tgacggaaat gtgatcagtc
      361 tggacagtgt ggcagatgag gctgaggatt ttgaactttc tgcagagctt atttctccga
      421 aaccggagaa ccttgcctat atgatctata cctcaggctc cacaggaaag ccaaagggtg
      481 ttatgatcga gcacagaaat ctgttgaacc tgatcgaata tatcacactg agcagaaata
      541 cttcaccgga tgacattgtg gcagagtttg cgagcttctg tttcgatgcg tcagtgatcg
      601 atctgtttgc acctcttacc gcaggagcag tcctgtatat tttaccggag agtatccgta
      661 aggacgccat tgccataagc agatatataa aggaaaaaga gatcaccacc gtaaccttcc
      721 ctacccagat gggagaactg gttacagaac ttctggagga tgcacctgct cttaaatttg
      781 ttacacttgg cggcgagaaa ttcaagcatt accgtaaccg tacctatcag atgatcaatg
      841 gctacggtcc tacagagaat acggtttctt ccacagagtt tttggtagac agacagtatg
      901 acaacatccc gatcggaaaa tcacagcgga atgtgcgcag ctacattgtg gatgagaatc
      961 tgaaccggct tccggtgggt gcgtccggcg aactttgtca cgcaggaagg cagattgcca
     1021 gaggatatca taatcttccg gaaaaaacag catctgtgtt tgtagaaaat ccatttgctg
     1081 tatgtgagca ggaaagccgt ctttacagga ccggtgatat ggtccgcatg aaaggcgacg
     1141 gaaatattga gtacataggc cggatcgatt cacaggtgaa gatcagagga tatcgtgtgg
     1201 agcttggtga gatcgaaggt gcccttctga aacatgagct ggtgaaaaat gcagccgtta
     1261 cagtaattga aaagggtgga aataaatata tcacagctta ttatacagga gaagccatcc
     1321 cggaggatga actgaagacc ttcctggagc cgctgatccc ggattatatg atgccgtctt
     1381 tctttgtcag cattgaggaa atgcctgtga caccgggcgg taagattgat aaaaaagcac
     1441 tgccaatgcc ggaggtaacc acgaacactg ccagctatgt agagccagtt acagcagctc
     1501 agagagcact ctgtgagatt tttgagaaag cactgggaat tgaaagagta gggattgagg
     1561 acaacttctt tgaactgggc ggatcttccc ttactgcatc caaggttgct gttatgtgtc
     1621 tgtcaaagaa tatttcactg gtatatgcgg atattttcaa atatcctact gtacgggaac
     1681 ttgcagctct ggttgatgat gacggagtta cagaggctgc acagagcaag aatgaatttt
     1741 cggattataa ctataacagg atccagaatg tgatcagcgc aaatacagaa gaaaatgcag
     1801 accggatcac aaaggaaaag ctgggagata ttatggtcac aggtgccaca ggtttcctgg
     1861 ggatccatgt ccttaaagca ttccttgaca attatgacgg taaggtttac tgtcttgtac
     1921 gtaagggcaa atatgaatcg cctgaaaagc gaatgatgaa catgctgatg tactattttg
     1981 atgatccata caaagagctg tttgaaagcc gtattatctg tgtggatggg gatattacct
     2041 caaaggaaca ggtaacagga ttttcagaat acaaattcag caccatcatt aactgtgctg
     2101 cctgcgtaaa gcattttgcg gctgatgatg tactggaaag aattaatgtg cagggtgtgg
     2161 aaaatctgat cgatttctgt aagaataacg gccgcagact gatccagatt tctacggttt
     2221 ccgttgcggg agagggttct gacggtgttc cgccaatgtc cagagttttc tgtgaaaatg
     2281 atctgtacat cggacagaat attaccaacg aatatatccg taccaaattc ctggcagaga
     2341 gggcggttct ggaagcagtt tccgagggac tggacggcaa ggtagtccgg gtaggtaatc
     2401 tgatgagccg aaactctgac ggcgagttcc agattaattt cattaccaat ggattccttc
     2461 gcagcctgag aggttatgcg gctgtaggta aattccctat gggcggtatg catgaggtgg
     2521 cagaattctc accgattgat tctactgcgc tggcagttct gagactggct cagaccgacc
     2581 ggagatttac tgtattccat gcatgcaaca gccatcatat ttatatggca gacctgatct
     2641 acgcaatgcg cagctatgga ttcaggattg atattgtaag agatgaggag tttgaggcag
     2701 cagtgaagga atttgcaaag accggtcagg attctgatgc ggtttccgga ctgatcgcct
     2761 atacctcaca taatgaaaat gaaatctata ccattgatta cagcaatcgt tttacagcac
     2821 aggttctgta caggctggat tataaatggc cggttacaga tgacagatat ctggaaaatg
     2881 caattgcagc acttgacaga cttacatttt ttgattgatt caggagggaa aattacaatg
     2941 gaaattatca caaaaacaga aggtaacaaa gcaactatgg aaatcagcgg atggcttgat
     3001 acacagacag ctccccagtt aggggaggct ctgtccggac ttgaagacag catcacaagc
     3061 ctggtatttg attttacaaa tctggagtat atttcatctg caggcttacg tcaggtgatc
     3121 gtagcctaca aaaaaatggc tcaaaaggat ggatttaaga tcatcaatgt ttctgatgag
     3181 gtttacgatg tattcagcct tacagggttt gacagcaaga tcgatattca gaagaagtaa
     3241 ctgttgatca gatgcagaaa ttatacagga tcaatattaa tcattttgag gatccgctta
     3301 agaatgaaag gcttctggat ctggttggga tcagaagaag agaaaaggtc atcaggtatc
     3361 gtatgcctga tgaccgcaaa agaagtctgg gtgcagggat catcatcaga aaaatcctcg
     3421 atgaaaacgg gcttacggaa agctgcctga gatattctga caatgaaaag ccggttgtgg
     3481 atggcctttt ttttaatgta tctcatgcgg gggactatgt tgtgggtgtg ctgtcagact
     3541 gcgaagtcgg gtgcgatatt gagaaaaatg ccaatgcgcc tcttgaggtg gcagaacatt
     3601 atttctacca ttcagagctt gcttacatca aggctgcgaa gaacaaagat aaggcgtttt
     3661 ttacgctctg gacattaaaa gaaagttata tgaaaatgac aggcagagga atgagccttc
     3721 cttgggattc ttttgaagtg gttc
//
