LOCUS       c00225_gnlPro..         3783 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LPHEGECM_225.
ACCESSION   c00225_gnlPro..
VERSION     c00225_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-08 00:49:04
            Original ID  :: gnl|Prokka|LPHEGECM_225
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3783
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3783
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1731:3781]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1732..3781
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3783
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3783
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(2..1228)
                     /locus_tag="ctg225_1"
                     /transl_table=11
                     /translation="MWEAINSFLSTVDNLVWGVPLMALIMAGGILLTVRLGLLQMRRLP
                     LALKWMFKNEEEGDGEISSFAALCTALSATIGTGNIVGVATAVCAGGPGALFWMILAAF
                     FGMATKFSEGLLAVKYRVVAEDGHSLGGPFYYIEKGMGTKWKWLAKIFAFFGVCVGLFG
                     IGTFSQVNGISSAVQNFFDPNKSNCVNIPFLGEYSWAVVISSLILAACVAAILIGGLKR
                     IATVSQIIVPFMAVIYLIFSVALILLNVTEIPAAIVTVVKGAFNPSAVTGGVVGTMIVS
                     MQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTMTGLSIVL
                     TGAWQVEGLEGVQVTTYAFQHGLPIPGQISAFVLMICLVFFAFTTILGWDYYSERCLEY
                     LTNGHKKTIL"
     CDS             complement(1732..3411)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_2"
                     /sec_met_domain="HGD-D (E-value: 4e-18, bitscore: 53.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 2.8e-66, bitscore:
                     211.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKIYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYILDVLHCDSACSRER
                     MAAQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADA"
     CDS             complement(3404..3781)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_3"
                     /sec_met_domain="HGD-D (E-value: 1.1e-22, bitscore: 68.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNGSALRR
                     IEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRSHGG
                     EGQTSTRLGAFLEMLGGAENE"
ORIGIN
        1 taaggattgt tttcttatgt ccgtttgtca gatattccag acatctttca ctgtaataat
       61 cccatccaag aattgtagta aatgcaaaga ataccagaca gatcattaat acaaatgcag
      121 aaatctgtcc aggaatcgga agtccgtgct ggaatgcata ggttgttacc tgaacacctt
      181 ccagaccttc tacctgccat gcacctgtca gtacgatgga aagacctgtc attgtacaga
      241 tcacgatcgt atcaataaat gtaccagtca tgcttacaag accctgacgt acaggctctt
      301 ttgtctgagc tgctgccgct gcgataggtg cgctaccaag acctgcttca tttgaaaaaa
      361 taccacgagc cacacctttc tgcatggata cgatcattgt tcctaccaca ccgcctgtca
      421 ctgctgacgg attaaaggct cccttcacaa ctgttacgat agctgccggg atttctgtaa
      481 cgtttaaaag aatcagcgct acactgaaga tcagatagat caccgccata aacggtacaa
      541 taatctggct gactgttgca attcgtttca gaccaccgat gaggattgct gcaacacagg
      601 ctgcaagaat cagggaagaa attacaactg cccaggaata ctctccaagg aacggaatat
      661 ttacgcagtt tgatttgttc ggatcaaaga agttctgaac tgcactggaa ataccattta
      721 cctggctgaa tgtaccgatt ccgaaaagac ctacacatac accaaagaat gcaaagatct
      781 ttgcaagcca tttccatttt gttcccatac ctttctcaat ataatagaaa ggaccgccaa
      841 ggctgtgacc gtcttctgct accacacggt atttcactgc aagaagacct tcggaaaatt
      901 ttgttgccat tccgaagaat gctgcaagaa tcatccagaa taaagctccg ggacctccgg
      961 cacagacagc agttgcaaca ccgacgatat taccggttcc gattgtggcg ctcagtgctg
     1021 tacataaagc tgcaaagctg gaaatctcac catcgccttc ttcttcgttc ttaaacatcc
     1081 atttcagtgc aagagggagt cttctcatct gaagaagtcc aagtctgaca gttaacaaaa
     1141 taccacctgc catgatcagg gccataaggg gcacacccca gacgagattg tctacagtag
     1201 ataaaaatga attgatcgct tcccacataa taataaatct cctttttctt ttaaaaagtg
     1261 taatatattg tacagctggg aaacaaagta ataacagacc gtttcaaaaa tcagaagttc
     1321 agaaaatact gttcatatag ttcatatgaa tcagcgggga tgataaagca tcctctgtcc
     1381 ttttgcctga gagattcaag acacgaatca gataaccatt tgtgggtagc tattgataat
     1441 tcgtgttatt tgcaccttcg gcccccgatc taacgggtgt ctccagagaa acgatccatt
     1501 tgcagttttt ccctgcatcc agggaacctg agagttttac tccttcggcg ggtatagcct
     1561 tttgtgtggc ttcctctttc ctgcaaactt cacctcgtta atttgctgtt actgtggtat
     1621 actatagctc ttaaatttga actcgtcaat agaaaattaa acttttataa aaaaagtata
     1681 aaaattccag ggatttccac ctcgtaaaat ccccggatat ttaaacgttt tttacgcatc
     1741 agccatatca gccgcaaaca gtgctgcgcc aagtgcaccg caaagctgag ccttgtcact
     1801 gatcaccagc tttgttccaa gcttctcttc cagtgtcttt accaggccct tattcttaga
     1861 aacaccaccg gtcatcatat atttctcctc accgcccaca cgtcttgtaa gggcggcagt
     1921 ctttacggca accgccttat tcaggccatg gacaatgtca tctgttgcct tattctgggc
     1981 gatcagggaa acaacttctg attctgcaaa tactgtacac atactggaaa ttgtaatatc
     2041 ttctttgaat tccagcccac attcgctcat ctgatccaga ttcatttcca tggtacgtgc
     2101 catcatttcc agaaatcgtc cggttcctgc cgcacatttg tcattcataa caaagtttgc
     2161 aactgcaccg ttctcatcca gtcggatcac cttgctgtcc tgtccgccaa tatcaataac
     2221 agtacggact tccggattca ggaaatgcgc accccttgca tgacaggtga tctctgtgat
     2281 actcttatca ccatttttaa tagctgtacg tccgtatccg gtagtaacca gcgcatcaat
     2341 atcttctctc tgcaatccgg cctccttcaa tgcctctgca agggcacggt ctgctccgat
     2401 agcagctcct gctcctgtag gcaggataat actggtcaca atctcatgat ctttatttaa
     2461 aatcaccaca tctgtactgg tggaaccact gtcaatccct gcgaaataac cttttcccat
     2521 ttttctctct ccttttgtgt ctccatctat ggtctgctcc agtgtttcag gctgaatgct
     2581 ctctgcaaaa gcttccagtc tggtaagaag ctgaccgctg ctctgaatag tataatctga
     2641 ttcaattttt aaaagaggta catctgtatg gttcttgatc tcagcatatt cgaagctgta
     2701 aaaatcacaa aacttcactg tatgataaat aatccccgca acagacggat cattataaag
     2761 cttcttacga cctgtcgggt ccatcatacg catacatgga atctgcccca gaatctcacc
     2821 tgcataccag tccatcagct catcaaaaga agcaccctcc ggtggaagta tatttcccac
     2881 agaacggtta tgcacgcagg tatcattctc tacaggaaga ggcatagcct tgctggtcat
     2941 ttcaaataat tcctgcccca ttctggcgcc aagcactgca atatgggact ttgtgatttt
     3001 ctcctgtgca tggaaagcag cccggaattt tccggcatca aactctgtgc ctttgtactg
     3061 tgcataagcc ttggccagac cttttaactg tgcagccata cgctctctgc tacaggcact
     3121 gtcacagtga agcacatcca aaatataaag gaaatccagt ttaccgcttt ccagcaaaat
     3181 gtcatacaca cttcggatgg tatcacagca gctcaccaga acaagttcct tgattttccc
     3241 ctccatgact ccttccagaa gtgtttttcc aaaaccgcag atattgggat gtgaaacctg
     3301 atcggccagg tcaaatccct ccggcatatg attaaagttc tcacattccc cgccaagaga
     3361 ttccagaagt tcaattgggg tatatttaca gacatagtaa attttattca ttctcagcac
     3421 ctcccagcat ttccagaaaa gctcccagcc tggtggaagt ctgtccttct cctccatgac
     3481 ttcgatcaca cccgtcaccg tccagaatca aaaggggcag accggcttcc tcgaacttct
     3541 tctttgcaag ctgtgccgca ccaagcgtat gtttacatcc ccaatgtcca aaccacacag
     3601 caccgtctgc tcctgcctgt ttcgcatggc ggataccggc ttcaatcctt ctaagggcac
     3661 ttccgttcag tgaatgatat accattcttt ctgccattgc ttcaaagggg ttctcaggat
     3721 caaaatccgg ctcacaggtt tctgcaagtt cacatcctac gatctgggct ttctcactga
     3781 agc
//
