LOCUS       c00226_gnlPro..         3770 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LPHEGECM_226.
ACCESSION   c00226_gnlPro..
VERSION     c00226_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-08 00:49:04
            Original ID  :: gnl|Prokka|LPHEGECM_226
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3770
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3770
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1729]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1729
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..3770
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3770
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             complement(3..872)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg226_1"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 3.5e-19, bitscore:
                     57.6, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 3.9e-37,
                     bitscore: 115.2, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLLVIGCGGVASVAIRKCCQNSDVFTEIMIASRTKEKCDALKE
                     KIESTTKTKIETAKIDADNAAEVAELIRAYKPDAVLNVALPYQDLTIMDACLEAGADYI
                     DTANYEAEDTEDPTWRAIYEKRCKEKGFTAYFDYSWQWAYDEKFKEAGLTALLGTGFDP
                     GVTSVFSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWEDGH
                     WVETEPMEFKSVYDFPEVGKKDMYLLHHEEIESLAKNIPGVQRIRFFMTFGQSYLTHMK
                     CLENVGMLS"
     CDS             complement(869..1729)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg226_2"
                     /sec_met_domain="Spermine_synth (E-value: 2.5e-54,
                     bitscore: 171.2, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 1.4e-16,
                     bitscore: 48.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELWYSEFHTGNVKLSVRINRQLFSGESEFQRIDVFESEEFGRFV
                     ALDGEIVFSDKDEFIYDEMVTHVPMTVHPNVKNVLIIGGGDGGVARELIHYPQIESIDV
                     VESDKMFVDVCAEMFPDIAQGLKDERVNIYYEDGLRFLRNKKARYDLIINDSTDPLGHT
                     EGLFTKEFYGSCYTALRDDGIMVYQHGSPFYDEDEVECRKMHRKVFRSFPVSRVYQAHI
                     PTCPSGYWLFGFASKKYHPLEDFRPERFDNLNIETWYYTTNLHRGAFMLPKYVEDLLEE
                     EENSL"
     CDS             complement(1732..3189)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_7_arginine"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg226_3"
                     /sec_met_domain="OKR_DC_1_C (E-value: 7.5e-15, bitscore:
                     43.2, seeds: 113, tool: rule-based-clusters)"
                     /sec_met_domain="OKR_DC_1_7_arginine (E-value: 1.7e-127,
                     bitscore: 413.0, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYERQQHAPIYEALERFRKKRVVPFDVPGHKRGRGNPELAQLLG
                     EKCVGLDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSAVQSMILSVC
                     KAGDKIILPRNVHKSAINALVLCGAIPVYVNPEVNAQLGISLGMEVSQVEKAMDENPDA
                     VAVLVNNPTYYGICSDLRTIVKKAHARGMKVLADEAHGTHLYFGRNLPVSGMAAGADMA
                     AVSMHKSGGSLTQSSLLLLNKSMNAEKVRQIINLTQTTSASYLLLSSLDISRRNLALRG
                     EESFEKVAGMAQYAREEINEIGGYYAYGMDLINGTSVFDFDVTKLSIYTLGNGLAGIEV
                     YDLLRDEYDIQIEFGDICNILAYISIGDRLQDIERLVGALADIERLYKKDSTGMLSGEY
                     IAPAVVASPQQAFYAEKESLPMEQAAGRISGEFVMCYPPGIPILAPGEMVTQEIVEYIL
                     YARDKGCSMQGMEDPKVEHLQVLKGGI"
ORIGIN
        1 gtggaaagca ttcctacatt ttccagacat ttcatatgtg tcagatagct ctgtccgaat
       61 gtcataaaga aacggatcct ctgtactccc ggaatatttt ttgccagtga ttcaatctct
      121 tcatgatgaa gaagatacat atccttcttt cctacctcag ggaaatcgta gacagattta
      181 aattccatag gctctgtttc tacccaatgt ccgtcttccc agtaggaacc atttgcggaa
      241 acctctcgaa gattgatctc cgggttaaag tttgtggcaa aaggatatcc atgatcaccg
      301 ccgttacagt ccagaatatc aatggtatgg atttcatcaa aataatgctt caaggcatag
      361 gcactgaata cactggtaac acccggatca aaaccggttc ccagaagagc agtcagtcct
      421 gcttccttaa atttctcatc ataagcccac tgccaggaat aatcgaaata tgcagtaaag
      481 cctttttctt tacatctctt ttcgtagatt gcacgccatg tgggatcttc tgtatcctct
      541 gcttcataat tggcagtatc aatataatcc gcaccggctt ccagacatgc atccatgatg
      601 gtaagatcct gatatggaag tgccacattc aggacagcat ccggcttata ggctctaatc
      661 aattctgcga cctctgctgc attatccgca tctatctttg ctgtctcaat ttttgtcttc
      721 gttgtacttt caatcttctc tttgagtgca tcgcattttt cctttgtcct gcttgcgatc
      781 ataatctctg taaaaacatc gctattctga cagcatttgc gaattgccac tgaagctacc
      841 ccaccacatc cgattaccaa taatctgctc ataaactgtt ttcctcctct tccagtaaat
      901 cttctacata ttttggaagc ataaatgctc ctctgtgaag gtttgttgta taataccatg
      961 tttctatatt aagattatca aacctctccg gcctgaaatc ttccagtgga tgatatttct
     1021 ttgatgcaaa tccaaaaagc cagtatcccg agggacaggt agggatatgt gcctgataaa
     1081 ccctgctcac cggaaatgac cggaaaacct ttctgtgcat ctttctgcat tccacctcat
     1141 cctcatcata aaaaggactt ccatgctgat aaaccatgat tccgtcatct ctgagagccg
     1201 tataacagct tccgtagaat tcctttgtaa aaagtccctc tgtatgtccc agcggatctg
     1261 tggaatcatt gatgatcagg tcatatctgg cctttttatt ccgcaggaac cgaaggccgt
     1321 cttcataata aatgttgact ctctcatcct tcagtccctg ggcaatatcc ggaaacatct
     1381 ccgcacatac atccacaaac attttatcgg attccaccac atcaatgctc tcaatctgtg
     1441 gataatggat cagttctctg gcaactccac cgtcaccgcc gccgatgatc agaacatttt
     1501 ttacattggg atgtactgtc attggcacat gcgtgaccat ctcatcataa ataaattcat
     1561 ctttatcaga gaatacgatc tcaccgtcca gagccacaaa tcttccaaat tcttcagact
     1621 caaacacatc gattctctga aattcacttt cccctgaaaa taactgtcgg ttaatccgga
     1681 cagaaagctt tacatttcct gtatggaact cagaatacca taattccatg atcagatacc
     1741 ccctttcagg acctgaagat gttctacctt cggatcctcc attccctgca tggaacagcc
     1801 tttgtctctt gcataaagaa tgtattctac aatttcctgt gtcaccattt ctccaggagc
     1861 aagaatcggg attcccggag gataacacat aacaaattca ccgctgatcc tgcctgccgc
     1921 ctgttccatg ggcagagatt ctttctccgc ataaaatgcc tgctgtggag acgccaccac
     1981 tgcaggtgcg atatattccc cggaaagcat acctgtgctg tccttcttat acagacgttc
     2041 aatatcagcc aatgccccta ccagtctctc aatatcctga agcctgtctc cgatggaaat
     2101 gtaagccaga atattacaga tatctccaaa ctcgatctga atatcatatt catctctgag
     2161 aagatcataa acttcaatac ctgccagccc atttcccagt gtataaatgg agagttttgt
     2221 aacatcaaaa tcaaaaacac tggttccatt gatcagatcc ataccatatg cataataacc
     2281 gccgatctca ttgatctcct ctctggcata ctgcgccatc cctgcaacct tttcaaagga
     2341 ttcctctcct ctgagtgcca gatttcttct ggagatatcc agactggaca acagaagata
     2401 agaagcgctt gtggtctgag tcagattgat gatctggcgc actttctctg cattcatact
     2461 cttattcaaa agcagcagcg aactctgtgt cagacttccg ccggatttgt gcatggaaac
     2521 tgccgccatg tcagcacctg ccgccattcc ggatacagga aggtttctgc cgaaataaag
     2581 atgggttcca tgagcctcat ctgccagcac cttcattcct cttgcatgag ccttctttac
     2641 gatagtccgc agatccgaac agattccata ataggtaggg ttatttacaa gaactgccac
     2701 cgcatccgga ttctcatcca tagccttctc cacctggctg acttccatgc caagggaaat
     2761 gcccagctgc gcattcacct ccgggtttac ataaaccgga attgcaccgc acagtaccag
     2821 ggcattgatg gcacttttat gtacatttcg cggcagaatg atcttatccc ctgccttgca
     2881 gacagacagg atcatgctct gcactgcaga tgtggttcct cctaccataa ggaatgcatg
     2941 cgctgcgcca aaagcatctg ctgcaagctc ctccgcttct ctgatcaccg aaaccgggtg
     3001 gcagagattg tccagcggct tcatagaatt aacatccagc cccacacatt tctctcccag
     3061 aagctgtgca agctccggat ttcctcttcc tcttttatgt cccggcacat caaagggaac
     3121 gactctcttc tttcggaaac gttccagtgc ttcatatatg ggcgcatgct gttgtctttc
     3181 ataatccatt tgacatgttc ctcttttcct gtcgtcatgt accggcaaat acagttctat
     3241 cattcagcaa ttgtatactg cctcaaaccg gcacaatgat tcaattattt ctatatatat
     3301 ttgctatatc tgcacaaaaa aagaagcaga taagttcatt caccatctgc ttctctcatg
     3361 atctatagaa tttctcaggc tctgtctctt tatatactga ccggatttcc cgtataaatc
     3421 cgtctgtatc ctgtgcctgt tcccgtattc tgaaatattc atgactgctg tcgtcttcaa
     3481 tcgctggcaa atatatgcta tgctcttatt gaccgtttca caagatgatg tgtgcttacg
     3541 cactgattat tataaagtaa tcaacttata aaatttgagc ttctaactca atcaataatg
     3601 tggtgtctcc cacccggcaa ctgccccgcc tgtccgtatg gcttaacatt cagaaactgt
     3661 ctctgaatgt gagcaaatat ttgcatgaaa acgatagtgt cttttcatac taaagatgag
     3721 aataacatat cttctgaact gttgtcaatt ccattttcca tattttgcac
//
