LOCUS       c00282_gnlPro..         7479 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MMMEGNHO_282.
ACCESSION   c00282_gnlPro..
VERSION     c00282_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 20:19:42
            Original ID  :: gnl|Prokka|MMMEGNHO_282
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7479
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7479
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[307:3266]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      308..3266
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7479
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7479
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(105..218)
                     /locus_tag="ctg282_1"
                     /transl_table=11
                     /translation="MLNSIKIPIYGEKPEGGYYFQIYFSNIFLRNNLQVKK"
     CDS             308..2536
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_2"
                     /sec_met_domain="Gly_radical (E-value: 2.5e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2541..3266
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg282_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.4e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEAGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFQTVQVN"
     CDS             complement(3499..4758)
                     /locus_tag="ctg282_4"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVLTTAVSLYFLNSPVADEIRYATAPSLAVNPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLVEKRPEAVTEEKRSEPQQEAIAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSENFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVVDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(4813..5361)
                     /locus_tag="ctg282_5"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDYFDKFTWRGEGSLRAWMERVMVNTALQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
     CDS             5549..6301
                     /locus_tag="ctg282_6"
                     /transl_table=11
                     /translation="MKKFNLITFAVILMLLPILQSCLDDANNDEWVTCPPGGILAIGTM
                     KIPNADTPRDFFIALDNGDNVLPADTADIRNRKYTVAEGQRVFVGYLQMGEKKPGYENG
                     KIFTIEDILTKEIIPLTEATADSIGDDRINVTAHALTKDYLTIEYQYLGSMNENKKHML
                     NLVQNEITGPIKDDGYIYLEFRHNAFNDSPNQLGSSLVSFKLDSIAEQLATAKGIKLRV
                     NTIYDNIQYVTIDINEDKNLKIKSFHSQ"
     CDS             complement(6406..7404)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     -3.5; E-value: 38)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg282_7"
                     /sec_met_domain="LysR_substrate (E-value: 1.2e-47,
                     bitscore: 150.8, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 5.2e-19, bitscore: 57.0,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVKLKISIFALIDSISLINQRTMTIQQLEYILAVDQFRHFAKA
                     AEYCRVTQPTLSAMIQKLEDELGVKLFDRSMTPVCPTAIGKKVLEQARKILSEVICVKE
                     IISEEQHSLSGTFRLAVLPTIAPYLLPRFFPQLMEKYPDLDIRVMEMKTPDIRKALLTG
                     EADAAIIASMLDDAALTEETLFYEQFLGYVSKKEPLFKHDVIRTSDVTGERLWLLDEGH
                     CFRDQLVRFCQMEAVKISQMAYRLGSMETFMHMVESGKGITFIPELAVMQLSEEQKELV
                     RPFAIPRPTRQIVLVTRKDFIRTSLLQVLKEEIQAAVPKEMLTLQAVQCLV"
ORIGIN
        1 cgcagtacaa cggttttcaa gaccgccgca atcgaccact ctgccacctc tccagaacta
       61 cggttaagta gtgcttttct cttaaagcgc tgcaaaggta cgaatcattt tttaacttgc
      121 aaattattcc gcaaaaaaat attagaaaag tatatttgga agtaataacc gccctctggc
      181 ttctccccat aaataggtat ttttatgcta ttcaacataa aataggaccc ttttatatac
      241 ctaaaccgaa aaaaggttgt aaatttgcgc gctctaaagg ataactaacc actaactgat
      301 taattttatg gaactgaata agacctttaa agatggactt tggagcaaag aaatcaatgt
      361 aagggatttc gttagtaaca acatcactcc ttatgaaggg gatgcttctt tcctgcaagg
      421 acctaccgaa cgcaccaaag ctgtctggaa tcactgtctc aaagctcttg aagaagagag
      481 aaacaataat ggtattcgtg cactcgacta cacaactgta tctactataa cctctcaccc
      541 tgccggctac atcgataaag agaacgaact gatcgtcgga ctacagaccg atcaggtgtt
      601 aaaacgtgcc atcaaaccgt ttggcggaat caatgtggtt atgaaagctt gccgtgaaaa
      661 cggagtggaa gtagatgaca gagtcaagga tatcttcact cactatcgca agactcataa
      721 tgatggggta ttcgatgtgt ataccgaaga aattcgttcg ttccgttcat tgggattcct
      781 taccggactt cccgacaact atgcacgcgg acgtatcatc ggtgattatc gccgcctggc
      841 tctctatggt cttgaccgtc tgatagaagc taaaaaagaa gatctgcata atctgacagg
      901 tcctatgact gaagcccgca ttcgtcttcg tgaagaagta gccgagcaaa tcaaggccct
      961 gaaagagatt aaagtattgg gcgaatatta tggtttggat ctgagccgac cggcatatac
     1021 cgcacaggaa gctgtacaat gggtgtatat ggcttatctg gctgccgtaa aagagcagga
     1081 tggtgctgcc atgtcactgg gtaatgtatc ttcattcctc gacatctatc ttgaatatga
     1141 attaagccag ggtacgataa ccgaaacatt cgctcaggaa ctgatcgacc aattcgttat
     1201 caaactccgt atggtacgcc acttgcgtat gcagtcgtac aacgatattt ttgcgggtga
     1261 tcccacatgg gtgaccgaat ctatcggtgg acgtttcaat gatggacgta ccaaagttac
     1321 taaaacttct ttccgcttct tgcagacact ttataactta ggcccatcac cggaacctaa
     1381 catgactgta ctatggagcc ctgagttacc cgaaggtttc aaagcattct gtgcacaagt
     1441 atctatcgac acttcttccg tacaatacga aaacgacgac ctgatgcgtg aagtccgtca
     1501 atcggatgac tacggaatcg cttgttgtgt ttcatatcag gaaatcggaa agcagattca
     1561 gttcttcggt gcacgtgcca accttgccaa agccctgcta ctggctatca acggaggacg
     1621 ttgcgaaaat acaggtacag ttatggtaaa agacatccct gtattgaccg gtgaaacact
     1681 gaagttcgaa gaagtaatgg ccaactataa gaaagtattg atacaaattg cacgtgtata
     1741 caacgaagca atgaatatca tacattacat gcacgataag tattactacg aaaaggcgca
     1801 gatggctttc gtggatactg atccccgcat taacctcgct tatggtgtag ccggactttc
     1861 aatcgccatc gattcactgt ccgccatcaa atatgcacat gtgaaagccc gtcgcaacga
     1921 catcggactg acggagggat tcgatatcga aggtgctttc ccttgtttcg gtaatgacga
     1981 tgaccgtgta gaccacttgg gtgtggacct ggtatatttc tttagcgaag aattaaagaa
     2041 actccccgta tataagaatg cccgtcctac cctttcgctg ctgactatca cttcgaacgt
     2101 aatgtatggc aagaagaccg gtgccactcc tgacggacgc gccaaaggca ttgcctttgc
     2161 tcccggtgcc aaccccatgc acggacgtga taaaaatggt gcaatcgcct cactgagttc
     2221 tgtagccaag ttgcgttatc gcgactcaca ggatggtatc agcaatacat tctcaatcgt
     2281 tcccaaatca ttgggagcaa cacaagaaga ccgtatcgac aatctggtga ccatgatgga
     2341 cggatatttt acaaaaggcg cacaccacct gaacgtaaac gtgctgaacc gggaaatgtt
     2401 gcaggatgcg atggaacatc ccgaaaagta tccgcaactg actatccgtg tctcaggata
     2461 tgccgttaat ttcgtgaaat tgagccgcga acatcagttg gaagtaatca gccgcagttt
     2521 ccacgaaaga atgtaattga atgataaacg tacactcata cgagtcaatg ggaacattcg
     2581 acgggccggg tctccggctc gtcgttttcc ttcaagggtg taacttccgt tgcctctatt
     2641 gtgccaatcc ggacacaatt gaagccaaag gagggacagc aaccgaccct gaggaaatag
     2701 ttcgcatggc cgtcagtcag aaagctttct tcggtaaaaa gggaggtatc accttttccg
     2761 gcggagagcc tacttttcaa gcaaaatcgc ttatcccact ttttaaaaga ctaaaggaag
     2821 caggaatcca tatttgcctt gataccaatg gcgggctatg gaacaatgat gtagaggaac
     2881 tattggaatt gactgatctg gttttattag atatcaaaga atttaatccg gaacatcatc
     2941 agtctttaac cggaagaagc aacgagcaga ccctgaaaac cgcagcctgg cttgaaacca
     3001 atcataaacc attctggttg cgatacgtat tagtgcccgg ctacagtgac ttcgaagacg
     3061 atatccggca gctgggagag catcttggaa cgtaccagat gattcagcgt gtagaaatat
     3121 tgccttacca caccttgggt gttcacaaat atgaagcaat gaacaaagaa tatatgctga
     3181 aaggagtgaa agagaatact ccagagcaga tagaaaaggc tgagaaacta ttcaggcaat
     3241 atttccagac cgtacaagta aattgactta tataagtgtc gaaactcgta ttcgccacag
     3301 attacacgaa tttacacaga taaagattca ttggccatca atagattaag ttcactctgc
     3361 ggtgaattat gacgactgca cttatttata attttgtaag tcacgatata aaagtctccc
     3421 tgttcttgcc ttaattcatt acaatacaag gcaagaacag ggagataatg taaccggcat
     3481 attttttgcc accctacttt aataagtgaa tcgcaatcct gcctgcaagt taaagttaca
     3541 aggcctttct tttcggatgg tttgtacttt ggagccatca tcaaagaaat acgaaactcc
     3601 cggttccaca tataggccga catgatctgt cgcattgaac tgcgctccca cggcaccggc
     3661 tacagaaaat tgcaaaggct tcacattcac cttatcatcc gccaactttc cataaacaca
     3721 cttttcaacc attcctccgg cagatacata gagtgtaaaa tatttctttt ccagaaaatt
     3781 ccagtttcca cgtacaggaa tacctatata atgcaatttc tgactctgca tcttggtatc
     3841 cccctgccgt ttgatatcag aagacaacaa cgtataagtc aatcccgtct ctacagagaa
     3901 acctttggga agagacttac gtacagacag tccgaagcta accggctgat gatgttcata
     3961 atccaccacg gcagttgtat tggacttcag gtaaggaacc ccttcctcaa atataacttc
     4021 ctgatcggca ggaatactca cagctccatt catgatcgtc atcaagtcga ccctgttagt
     4081 catcggacgg ggcgcaaaat tttcggaacc atttgtcgga agtccacctc cattaccgat
     4141 acccactccc atcgaccatt taccaccacg tttagcagaa gagtctccga taggcaactg
     4201 atacttgtct ttaccggacg gtcgccgcgc ttctttcctc tttggaggct gcgccgtcgc
     4261 actttctttt ttctcgattg cagcaattgc ttcctgttgc ggctcagatc tcttttcctc
     4321 cgtcactgcc tccggtcgct tctctaccag ggaggatacc tccggaacaa tcaccggatc
     4381 agtattcttt gccaaatagc ccgaaacagg gttaatactc gtcgttccta ccggcttaac
     4441 tggttcggat acagctacct gtacatccgg caaggcaggt tccggcaata catccgggtt
     4501 gacagccagt gaaggagccg ttgcataacg gatttcatca gctaccggac tattcagaaa
     4561 atacaaactg acagcagtcg ttagcaccaa taccacagct gccgcagcaa ccgcccaccg
     4621 gcgatacgga tatattcgtt tttcggttac aggcatcagt tccctttcca gtcgttccca
     4681 accggaagca ggcatcggct ccgaataatc ttcgagctta tccttgaatg ctttaatcca
     4741 tttttcgtct tctttcatca ttctatttca tttttgaaat ccaaggttca cctcagtatc
     4801 tgtaaataca tgtcaaccat tggtcaccaa ccattctttc actttctttg ccaatacact
     4861 ttttgcgcga aaaagctgtg aggcagaaga tttttcatta atccccaaca tctgtgcgat
     4921 ttccttgtgc gacttatctt caaaggtgta taaattgaat acggtacgat atccggcggg
     4981 gagttcatta ataaattgca tcagcacttt ctgtggtatt gcttcaacag ccgaagcatc
     5041 cggttcttca tacgtttcgg gaacttcatc cagtgccgtg gtctggttca tcacgtcgtt
     5101 ctttcttaag aactgtaaag ccgtattcac cattacacgc tccatccatg ctctcagaga
     5161 gccttcaccc cgccaggtaa acttgtcgaa gtagtcgaaa atcttcagga aaccgtcgtg
     5221 taccaagtct tgtgccatat ccctgtctcc ggcataacga agacagacac caagcatgcg
     5281 ccctgcgtac tgctcataaa gttctttgcg ggcacgatta tctccctgcc tgcaacgttc
     5341 cgacagctct aattcattca ttctcctaaa cacgtgttta ccttataaat gcagcaaaga
     5401 tagaaatact gcacagagag aacaactctt ttatcacttt tgtttcttta acattttaag
     5461 atgcaatata tcatcacttc ctgcattttc tcaatacaat aaagaataaa tctcaatgga
     5521 taaataggat aaaaccaaca agaaacttat gaagaaattt aatttaataa cttttgccgt
     5581 catcctgatg ttattgccca ttttacagtc atgtctggac gatgccaaca acgacgagtg
     5641 ggtcacttgt cctccgggtg gaatattggc catcggcaca atgaagattc ccaatgcgga
     5701 cactccaagg gactttttca ttgcattgga caatggagac aatgtacttc cggcagatac
     5761 ggccgatata cgcaacagga aatacactgt tgcagaaggt caaagagtct tcgttggcta
     5821 tctgcagatg ggagaaaaga aacccggata tgagaacggt aaaatattca cgatagaaga
     5881 tatattgacg aaagagatca ttccactgac ggaagctaca gcagacagca ttggagacga
     5941 ccgtatcaac gtcacagcgc atgcacttac gaaagattat ctgaccatcg aatatcaata
     6001 tctgggcagc atgaatgaaa ataaaaaaca catgctgaac ttggtacaaa acgaaataac
     6061 aggtcccata aaagatgacg gatatatcta tctggagttc cggcataatg cctttaatga
     6121 ctcccccaat caattgggtt cgagccttgt ttctttcaag ctggatagta tagccgagca
     6181 attggcgaca gccaaaggaa taaaactccg tgtgaacacc atttatgaca atatacagta
     6241 tgtcacaata gatatcaacg aagacaaaaa cttaaaaata aaatcatttc attctcaata
     6301 aaacaacaag aaacacctac acaatcaggc aaaacgagta ataattctga tacataaaag
     6361 aaataaggcg gcgatgtttg tgaaaatatc gccgtcattt ttatattata ccaggcactg
     6421 aacggcctgc agggtaagca tctccttggg aaccgccgcc tgaatctcct ctttcaaaac
     6481 ctgcagcaga ctggtgcgga tgaagtcttt ccgggtcacc aggacaatct gacgggtagg
     6541 ccgcgggata gcaaacggac ggaccagttc tttctgctct tcgctcaatt gcatcactgc
     6601 cagttcggga ataaacgtaa tccccttacc actctccacc atatgcataa aggtttccat
     6661 actacccagc cgatacgcca tttggctgat cttaaccgct tccatctgac aaaaacgaac
     6721 caactgatcg cggaagcaat gcccttcgtc aagaagccaa agccgttccc ctgtcacatc
     6781 ggacgtacga atcacatcgt gcttgaacaa cggttccttc tttgagacat agcccaggaa
     6841 ttgctcgtag aataaggtct cttccgttaa tgcggcatca tccagcatgc tggcaatgat
     6901 agcagcatcc gcttcaccgg tcagcagagc cttgcgaatg tcgggcgtct tcatctccat
     6961 cacccgtata tcaagatccg ggtatttctc catcagttgc ggaaagaaac ggggcagaag
     7021 ataaggtgca atcgtcggta acactgccaa tcggaatgta cccgacaagg agtgctgttc
     7081 ttcgctgatg atctccttga cacagataac ttccgaaaga atcttacggg cctgttccag
     7141 cactttctta ccaatggcag tagggcaaac cggtgtcatg ctacggtcga acagtttcac
     7201 ccccaattcg tcttccagct tttggatcat tgcactcaaa gtaggttgcg tcacccggca
     7261 atactcggca gctttagcaa aatgccggaa ctggtcgaca gccagaatat attccaattg
     7321 ttgtatagtc atcgttctct gatttattaa agatatagaa tctatcaatg caaagataga
     7381 aattttcagt ttgacacgaa tcattttgtg ccgtatcttt gttccagata aaaagaagaa
     7441 atatagtatt ctggtaatct gcgcaaccaa tatgagtac
//
