safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	28	44.05

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
IIKHECMK_1	43848	44687	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
IIKHECMK_1	1044703	1045493	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	100.00	100.00	-
IIKHECMK_1	862970	864628	fbp54	0.0	FBPs	Adherence	100.00	100.00	WP_011227179
IIKHECMK_1	1315186	1316037	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
IIKHECMK_1	1126113	1126874	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
IIKHECMK_1	967063	968484	STR_RS05200	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227236
IIKHECMK_1	968492	969415	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
IIKHECMK_1	969375	970325	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
IIKHECMK_1	970350	971432	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
IIKHECMK_1	971522	972496	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
IIKHECMK_1	972502	972981	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
IIKHECMK_1	973174	974292	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
IIKHECMK_1	974860	975819	STR_RS05240	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827042
IIKHECMK_1	975983	976582	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
IIKHECMK_1	976719	977468	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
IIKHECMK_1	977478	978170	STR_RS05255	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227247
IIKHECMK_1	978179	978910	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
IIKHECMK_1	978911	980365	STR_RS05265	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827043
IIKHECMK_1	1090756	1091349	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
IIKHECMK_1	1302708	1304453	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
IIKHECMK_1	1304455	1306155	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
IIKHECMK_1	1306173	1307375	STR_RS06895	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227428
IIKHECMK_1	1307375	1308181	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
IIKHECMK_1	1308181	1309122	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
IIKHECMK_1	1309119	1310267	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
IIKHECMK_1	964311	965369	waaB	0.0	Capsule	Immune modulation	100.00	100.00	WP_157822584
IIKHECMK_1	1090756	1091349	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IIKHECMK_1	1090756	1091349	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IIKHECMK_1	1304455	1306155	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
IIKHECMK_1	1307375	1308181	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
IIKHECMK_1	1308181	1309122	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
IIKHECMK_1	1306173	1307375	STER_RS07055	0.0	Capsule	Immune modulation	99.42	100.00	WP_011681436
IIKHECMK_1	977478	978170	STU_RS14640	0.0	Capsule	Immune modulation	99.42	100.00	WP_011226047
IIKHECMK_1	1309119	1310267	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
IIKHECMK_1	1091349	1092218	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
IIKHECMK_1	978179	978910	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
IIKHECMK_1	976733	977468	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
IIKHECMK_1	978179	978910	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
IIKHECMK_1	1302708	1304453	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
IIKHECMK_1	976733	977468	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
IIKHECMK_1	978911	980365	STU_RS14650	0.0	Capsule	Immune modulation	97.94	100.00	WP_011226049
IIKHECMK_1	1313806	1315065	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
IIKHECMK_1	978911	980365	STER_RS05320	0.0	Capsule	Immune modulation	96.92	99.79	WP_011681177
IIKHECMK_1	977478	978170	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
IIKHECMK_1	595961	597264	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
IIKHECMK_1	1595768	1596778	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.82	99.51	WP_002986042
IIKHECMK_1	1307375	1308181	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
IIKHECMK_1	149223	150506	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
IIKHECMK_1	1089281	1090327	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
IIKHECMK_1	1313111	1313809	STER_RS07095	2.93e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
IIKHECMK_1	974568	974804	STR_RS05235*	6.61e-119	Capsule	Immune modulation	100.00	100.00	-
IIKHECMK_1	1312291	1312757	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
IIKHECMK_1	1306638	1307375	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IIKHECMK_1	1129561	1130544	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IIKHECMK_1	708534	709607	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IIKHECMK_1	513725	514693	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
IIKHECMK_1	1477370	1479673	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IIKHECMK_1	1216422	1217903	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
IIKHECMK_1	737215	739461	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IIKHECMK_1	63479	64471	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
IIKHECMK_1	27366	28337	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IIKHECMK_1	1363540	1362389	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
IIKHECMK_1	1329067	1327085	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
IIKHECMK_1	1785332	1783854	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IIKHECMK_1	1113367	1111637	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.52	100.00	AAK34199
IIKHECMK_1	595961	597262	Eno	0.0	enolase	86.21	99.77	ABP90468
IIKHECMK_1	1770596	1768704	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
IIKHECMK_1	742386	740677	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
IIKHECMK_1	1637458	1638468	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IIKHECMK_1	886417	885056	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
IIKHECMK_1	1687789	1685897	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
IIKHECMK_1	581531	582421	bglB	2.25e-176	putative phospho--glucosidase	87.88	99.66	AAK33487
IIKHECMK_1	801784	800849	SP_0676	7.16e-168	transcription facot	81.73	96.89	AAK74821
IIKHECMK_1	739653	740510	metF	8.38e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IIKHECMK_1	1017003	1016299	covR	3.40e-130	responder protein	84.68	100.00	AAK71319
IIKHECMK_1	1182743	1182072	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
IIKHECMK_1	1017003	1016299	walR	2.43e-120	DNA-binding response regulator	80.85	100.00	ANC99504
IIKHECMK_1	300651	301337	CovR	9.75e-118	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IIKHECMK_1	746339	746989	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
IIKHECMK_1	746339	746989	ciaR	6.91e-114	two component system response regulator	83.87	96.02	AIK75708
IIKHECMK_1	64768	65163	SpxA2	3.59e-74	transcriptional regulator	90.91	100.00	ERL21461
IIKHECMK_1	150664	151164	RopE (M5005_Spy_1611)	9.57e-75	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
IIKHECMK_1	1477370	1479667	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IIKHECMK_1	1673328	1672309	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IIKHECMK_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found