safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
7	0	1	7.07

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
FIJEFOCF_3	1714696	1715901	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
FIJEFOCF_1	608767	610692	tet(Q)	ARO:3000191	tet(Q)	97.04	97.56	Z21523.1
FIJEFOCF_3	245423	246184	ErmB	ARO:3000375	ErmB	97.95	102.02	AF242872.1
FIJEFOCF_3	241407	242192	APH(3')-IIIa	ARO:3002647	APH(3')-IIIa	100.00	98.86	CP004067.1
FIJEFOCF_10	800	1990	tet(C)	ARO:3000167	tet(C)	100.00	100.00	AY043299.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
FIJEFOCF_3	248579	249445	ant(6)-Ia	Streptomycin	100.00	100.00	KF864551
FIJEFOCF_1	608767	610692	tet(Q)	Doxycycline, Tetracycline, Minocycline	100.00	100.00	X58717
FIJEFOCF_3	241403	242192	aph(3')-III	Kanamycin, Amikacin, Neomycin, Butirosin, Isepamicin, Lividomycin, Paromomycin, Ribostamycin	100.00	99.37	M26832
FIJEFOCF_10	800	1990	tet(C)	Doxycycline, Tetracycline	99.92	100.00	AF055345
FIJEFOCF_3	245456	246184	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.86	98.78	U18931

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
FIJEFOCF_3	248579	249442	aadE	aminoglycoside 6-adenylyltransferase AadE	EXACTX	100.00	100.00	WP_001255868.1
FIJEFOCF_3	1715926	1716435	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
FIJEFOCF_10	803	1990	tet(C)	tetracycline efflux MFS transporter Tet(C)	EXACTX	100.00	100.00	WP_001297013.1
FIJEFOCF_1	608767	610689	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	EXACTX	100.00	100.00	WP_004293868.1
FIJEFOCF_3	241404	242189	aph(3')-IIIa	aminoglycoside O-phosphotransferase APH(3')-IIIa	BLASTX	100.00	99.24	WP_001096887.1
FIJEFOCF_3	1714696	1715898	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
FIJEFOCF_3	245450	246181	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.18	99.59	WP_002292226.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FIJEFOCF_5	173207	171627	GroEL	0.0	folding of newly synthesized proteins, preventing misfolding and aggregation	80.64	96.70	BAA04222

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FIJEFOCF_1	0.0	1.0	0.0
FIJEFOCF_2	0.0	1.0	0.0
FIJEFOCF_3	0.0	1.0	0.0
FIJEFOCF_4	0.0	1.0	0.0
FIJEFOCF_5	0.0	1.0	0.0
FIJEFOCF_6	0.0	1.0	0.0
FIJEFOCF_7	0.0	1.0	0.0
FIJEFOCF_8	1.0	0.0	1.0
FIJEFOCF_9	0.0	1.0	0.0
FIJEFOCF_10	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.4.0 (Database: Jan. 2024 release)