Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 3 | 5.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPNAPNAK_1 | 494938 | 495984 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KPNAPNAK_1 | 494938 | 495981 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| KPNAPNAK_1 | 2605294 | 2606670 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.36 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KPNAPNAK_1 | 494938 | 495981 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| KPNAPNAK_1 | 2605300 | 2606670 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.09 | 99.78 | WP_003242953.1 |
| KPNAPNAK_1 | 492669 | 493187 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
| KPNAPNAK_1 | 1852804 | 1855401 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPNAPNAK_1 | 3508642 | 3509823 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| KPNAPNAK_1 | 3508178 | 3508627 | capC | 1.48e-113 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPNAPNAK_1 | 127987 | 129123 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| KPNAPNAK_1 | 19038 | 19907 | pdxS | 1.57e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| KPNAPNAK_1 | 1651679 | 1652455 | CodY | 3.30e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KPNAPNAK_2 | 3007 | 3193 | Col(SD853) | 187 / 194 | 87.17 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPNAPNAK_1 | 0.0 | 1.0 | 0.0 |
| KPNAPNAK_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KPNAPNAK_1 | 560477 | 607472 | False | Siphoviridae | VOG0275, VOG4602, VOG8483, VOG9667, VOG9667, VOG0806, VOG4322, VOG0650, VOG4552, VOG4600, VOG1309, VOG9093, VOG0189, VOG0044, VOG0536, VOG1336, VOG4658, VOG8569, VOG0397, VOG0044, VOG5437, VOG9071, VOG6517, VOG5447, VOG3631, VOG4972, VOG10038, VOG0796, VOG10227, VOG0720, VOG4564, VOG6778, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG0641, VOG10230, VOG10380, VOG6422, VOG3490, VOG4666 |
| KPNAPNAK_1 | 858885 | 886455 | False | Siphoviridae | VOG4552, VOG3547, VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG7884, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0207, VOG4586, VOG5852, VOG6163, VOG0801, VOG3462, VOG4619, VOG4856, VOG0804, VOG4705 |
| KPNAPNAK_1 | 1258194 | 1283257 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| KPNAPNAK_1 | 2172866 | 2179763 | False | Siphoviridae | VOG0918, VOG4551, VOG0025, VOG7476, VOG0322 |
| KPNAPNAK_1 | 2241999 | 2266226 | False | Siphoviridae | VOG7718, VOG3390, VOG5008, VOG8610, VOG7930, VOG0275, VOG5250, VOG9312, VOG4910, VOG3389, VOG5042, VOG6023, VOG9712, VOG4660, VOG10230, VOG8537, VOG0054 |
| KPNAPNAK_1 | 3143222 | 3178234 | False | Siphoviridae | VOG4707, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG0198, VOG4548, VOG0327, VOG4609, VOG4567, VOG0026, VOG1565, VOG0021, VOG1047, VOG7438, VOG0181 |
Phigaro v2.4.0 (Database: Jan. 2024 release)