Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 17 | 34 | 38.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IMFCMAMH_6 | 511352 | 512203 | rfbD | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_014621820 |
| IMFCMAMH_6 | 514627 | 515342 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.55 | 99.86 | WP_011681442 |
| IMFCMAMH_3 | 466379 | 467110 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011227248 |
| IMFCMAMH_3 | 466379 | 467110 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| IMFCMAMH_3 | 466379 | 467110 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.58 | 100.00 | WP_011681176 |
| IMFCMAMH_6 | 515688 | 516458 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 92.48 | 99.23 | WP_011681440 |
| IMFCMAMH_6 | 512322 | 513580 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.30 | 99.92 | WP_011681445 |
| IMFCMAMH_3 | 467111 | 468571 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.26 | 99.86 | WP_011681177 |
| IMFCMAMH_3 | 607509 | 608102 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| IMFCMAMH_3 | 608102 | 608971 | rfbA | 0.0 | Capsule | Immune modulation | 90.92 | 100.00 | WP_011681286 |
| IMFCMAMH_3 | 607509 | 608102 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| IMFCMAMH_3 | 607509 | 608102 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| IMFCMAMH_3 | 467111 | 468571 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.65 | 100.00 | WP_011226049 |
| IMFCMAMH_3 | 357738 | 359396 | fbp54 | 0.0 | FBPs | Adherence | 90.60 | 99.94 | WP_011227179 |
| IMFCMAMH_3 | 467111 | 468571 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| IMFCMAMH_3 | 465678 | 466370 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| IMFCMAMH_3 | 465678 | 466370 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| IMFCMAMH_3 | 465678 | 466370 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| IMFCMAMH_3 | 791017 | 792320 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| IMFCMAMH_6 | 421228 | 422067 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| IMFCMAMH_3 | 1265838 | 1266848 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.18 | 99.80 | WP_002986042 |
| IMFCMAMH_3 | 200242 | 201003 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| IMFCMAMH_3 | 606193 | 607239 | rfbB | 0.0 | Capsule | Immune modulation | 83.59 | 99.90 | WP_002263087 |
| IMFCMAMH_6 | 134479 | 135762 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.53 | 99.61 | WP_000107753 |
| IMFCMAMH_3 | 464998 | 465668 | STU_RS14635 | 1.65e-148 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| IMFCMAMH_3 | 464998 | 465668 | STER_RS05305 | 7.67e-147 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011681174 |
| IMFCMAMH_3 | 464998 | 465668 | STR_RS05250 | 7.78e-137 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IMFCMAMH_3 | 197552 | 196569 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| IMFCMAMH_3 | 209139 | 210212 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| IMFCMAMH_3 | 1123462 | 1125768 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| IMFCMAMH_3 | 61928 | 60447 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.32 | 100.00 | ABJ54480 |
| IMFCMAMH_3 | 880104 | 879136 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| IMFCMAMH_3 | 229260 | 231506 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| IMFCMAMH_6 | 25207 | 26178 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| IMFCMAMH_3 | 146761 | 153324 | PrtA | 0.0 | serine protease | 90.14 | 101.96 | ABJ55268 |
| IMFCMAMH_3 | 635768 | 634038 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| IMFCMAMH_6 | 497674 | 499662 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| IMFCMAMH_3 | 965808 | 964657 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| IMFCMAMH_2 | 161112 | 159634 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| IMFCMAMH_3 | 792320 | 791019 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| IMFCMAMH_2 | 145771 | 143879 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| IMFCMAMH_3 | 234425 | 232716 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| IMFCMAMH_6 | 59644 | 60735 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| IMFCMAMH_2 | 20101 | 21111 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| IMFCMAMH_3 | 373325 | 371964 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| IMFCMAMH_3 | 806739 | 805849 | bglB | 1.41e-176 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| IMFCMAMH_3 | 231693 | 232550 | metF | 3.01e-169 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| IMFCMAMH_3 | 273669 | 272731 | SP_0676 | 8.90e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| IMFCMAMH_3 | 128411 | 129082 | rr01 | 2.79e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| IMFCMAMH_3 | 500891 | 500187 | covR | 5.80e-126 | responder protein | 85.53 | 100.00 | AAK71319 |
| IMFCMAMH_3 | 238387 | 239058 | ciaR | 1.25e-124 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| IMFCMAMH_3 | 238387 | 239055 | CiaR | 2.30e-124 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| IMFCMAMH_6 | 290077 | 290763 | CovR | 5.13e-120 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| IMFCMAMH_3 | 500891 | 500187 | walR | 1.32e-119 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| IMFCMAMH_6 | 60908 | 61303 | SpxA2 | 6.02e-75 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| IMFCMAMH_3 | 384790 | 385191 | SpxA1 | 2.66e-69 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| IMFCMAMH_6 | 114027 | 114440 | GrpE | 4.75e-67 | protein grpE | 81.88 | 80.59 | ABP89267 |
| IMFCMAMH_6 | 313285 | 313572 | spr0479 | 1.62e-47 | essential gene | 85.42 | 98.97 | AAK99283 |
| IMFCMAMH_3 | 922815 | 922561 | spr1327 | 4.86e-43 | essential gene | 90.59 | 100.00 | AAL00131 |
| IMFCMAMH_2 | 112281 | 112544 | spr0175 | 8.89e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| IMFCMAMH_2 | 113106 | 113372 | spr0177 | 1.97e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| IMFCMAMH_6 | 135929 | 136420 | RopE (M5005_Spy_1611) | 2.96e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| IMFCMAMH_3 | 1123462 | 1125762 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.97 | 98.08 | MYN69560 |
| IMFCMAMH_2 | 56520 | 55501 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| IMFCMAMH_3 | 333800 | 334339 | pnuC | 1.71e-93 | NAD salvage pathway importer | 83.89 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IMFCMAMH_1 | 1.0 | 0.0 | 1.0 |
| IMFCMAMH_2 | 0.0 | 1.0 | 0.0 |
| IMFCMAMH_3 | 0.0 | 1.0 | 0.0 |
| IMFCMAMH_4 | 0.0 | 1.0 | 0.0 |
| IMFCMAMH_5 | 0.0 | 1.0 | 0.0 |
| IMFCMAMH_6 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)