Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 31 | 39.82 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HNNLDLLG_1 | 1212765 | 1213634 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| HNNLDLLG_1 | 1212172 | 1212765 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| HNNLDLLG_1 | 1419194 | 1420000 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681437 |
| HNNLDLLG_1 | 1212172 | 1212765 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HNNLDLLG_1 | 1414485 | 1416230 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_011227426 |
| HNNLDLLG_1 | 1416334 | 1417974 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 99.69 | 96.47 | WP_041827753 |
| HNNLDLLG_1 | 1420000 | 1420941 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| HNNLDLLG_1 | 1212172 | 1212765 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HNNLDLLG_1 | 959970 | 961628 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| HNNLDLLG_1 | 1420000 | 1420941 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| HNNLDLLG_1 | 43785 | 44624 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| HNNLDLLG_1 | 1416334 | 1417974 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 99.51 | 96.47 | WP_041827077 |
| HNNLDLLG_1 | 1417992 | 1419194 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011681436 |
| HNNLDLLG_1 | 1419194 | 1420000 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| HNNLDLLG_1 | 1417992 | 1419194 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011227428 |
| HNNLDLLG_1 | 1075460 | 1076191 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | WP_011681176 |
| HNNLDLLG_1 | 1075460 | 1076191 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| HNNLDLLG_1 | 1075460 | 1076191 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| HNNLDLLG_1 | 1074009 | 1074749 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| HNNLDLLG_1 | 1074009 | 1074749 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011226046 |
| HNNLDLLG_1 | 1414485 | 1416230 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 98.17 | 100.00 | WP_011681434 |
| HNNLDLLG_1 | 1427131 | 1427982 | rfbD | 0.0 | Capsule | Immune modulation | 98.12 | 100.00 | WP_014621820 |
| HNNLDLLG_1 | 1074014 | 1074749 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.10 | 98.13 | WP_011227246 |
| HNNLDLLG_1 | 1248956 | 1249717 | srtA | 0.0 | Sortase A | Exoenzyme | 97.77 | 100.00 | - |
| HNNLDLLG_1 | 1076192 | 1077652 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.60 | 100.00 | WP_011681177 |
| HNNLDLLG_1 | 1076192 | 1077652 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.44 | 100.21 | WP_011226049 |
| HNNLDLLG_1 | 542010 | 546905 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 95.79 | 99.96 | WP_000167699 |
| HNNLDLLG_1 | 541962 | 546905 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.67 | 99.78 | YP_001200536 |
| HNNLDLLG_1 | 541962 | 546905 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.65 | 99.78 | YP_001198331 |
| HNNLDLLG_1 | 1076192 | 1077652 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.62 | 100.21 | WP_041827043 |
| HNNLDLLG_1 | 1072588 | 1073953 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.39 | 99.85 | WP_011681173 |
| HNNLDLLG_1 | 1424754 | 1426028 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 94.98 | 99.92 | WP_011226331 |
| HNNLDLLG_1 | 1074759 | 1075451 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 94.66 | 100.00 | WP_011681175 |
| HNNLDLLG_1 | 1072586 | 1073953 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.44 | 100.00 | WP_011226045 |
| HNNLDLLG_1 | 1156057 | 1156835 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 93.06 | 99.37 | - |
| HNNLDLLG_1 | 1420938 | 1422083 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 93.02 | 99.74 | WP_011227431 |
| HNNLDLLG_1 | 1420938 | 1422083 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 92.76 | 99.74 | WP_011681439 |
| HNNLDLLG_1 | 683217 | 684520 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| HNNLDLLG_1 | 1712534 | 1713544 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| HNNLDLLG_1 | 1074759 | 1075451 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.43 | 100.00 | WP_011226047 |
| HNNLDLLG_1 | 1074759 | 1075451 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 84.85 | 100.00 | WP_011227247 |
| HNNLDLLG_1 | 1419194 | 1420000 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| HNNLDLLG_1 | 177479 | 178762 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| HNNLDLLG_1 | 1210697 | 1211743 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| HNNLDLLG_1 | 1063055 | 1063638 | SPD_RS01775 | 1.14e-146 | Capsule | Immune modulation | 83.11 | 96.04 | WP_001819836 |
| HNNLDLLG_1 | 1418457 | 1419194 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.96 | 60.29 | WP_012000441 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HNNLDLLG_1 | 541989 | 546902 | B9H01_RS04765 | 0.0 | glucan-binding protein | 97.13 | 99.70 | AUS90958 |
| HNNLDLLG_1 | 1252404 | 1253387 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.51 | 100.00 | AAK75326 |
| HNNLDLLG_1 | 792362 | 793435 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HNNLDLLG_1 | 1590734 | 1593037 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HNNLDLLG_1 | 601006 | 601974 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| HNNLDLLG_1 | 1317470 | 1318951 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| HNNLDLLG_1 | 815890 | 818136 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| HNNLDLLG_1 | 63422 | 64420 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| HNNLDLLG_1 | 27301 | 28272 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HNNLDLLG_1 | 1469345 | 1468194 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HNNLDLLG_1 | 1441147 | 1439159 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.39 | 100.00 | AE014259_2 |
| HNNLDLLG_1 | 1236207 | 1234477 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.22 | 100.00 | AAK34199 |
| HNNLDLLG_1 | 1918928 | 1917450 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| HNNLDLLG_1 | 683217 | 684518 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HNNLDLLG_1 | 1903740 | 1901848 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HNNLDLLG_1 | 821061 | 819352 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| HNNLDLLG_1 | 1754501 | 1755511 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| HNNLDLLG_1 | 989296 | 987935 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| HNNLDLLG_1 | 1804817 | 1802925 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| HNNLDLLG_1 | 668780 | 669670 | bglB | 1.39e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| HNNLDLLG_1 | 892923 | 891988 | SP_0676 | 7.69e-168 | transcription facot | 81.73 | 96.89 | AAK74821 |
| HNNLDLLG_1 | 818328 | 819185 | metF | 1.59e-164 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| HNNLDLLG_1 | 1114438 | 1113734 | covR | 7.05e-131 | responder protein | 85.53 | 100.00 | AAK71319 |
| HNNLDLLG_1 | 1298991 | 1298320 | rr01 | 3.13e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| HNNLDLLG_1 | 1114438 | 1113734 | walR | 3.33e-121 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| HNNLDLLG_1 | 331667 | 332353 | CovR | 1.05e-117 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HNNLDLLG_1 | 825015 | 825683 | CiaR | 2.10e-117 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| HNNLDLLG_1 | 825015 | 825686 | ciaR | 2.07e-115 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| HNNLDLLG_1 | 64706 | 65068 | SpxA2 | 3.26e-66 | transcriptional regulator | 89.26 | 91.67 | ERL21461 |
| HNNLDLLG_1 | 1000715 | 1001116 | SpxA1 | 3.92e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HNNLDLLG_1 | 1790104 | 1789340 | ilvC (SPD_0406) | 1.49e-178 | ketol-acid reductoisomerase (NADP(+)) | 95.29 | 75.00 | ABJ55451 |
| HNNLDLLG_1 | 178920 | 179420 | RopE (M5005_Spy_1611) | 5.13e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HNNLDLLG_1 | 1590734 | 1593031 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| HNNLDLLG_1 | 576517 | 577713 | AUS90997 (B9H01_RS07385) | 0.0 | site-specific integrase | 97.74 | 100.00 | AUS90997 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HNNLDLLG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)