safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
7	1	0	7.07

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
EEEDKGCD_1	3177131	3179056	tet(Q)	ARO:3000191	tet(Q)	97.82	97.56	Z21523.1
EEEDKGCD_1	1828235	1829137	cepA	ARO:3003559	cepA	100.00	100.00	U05883.1
EEEDKGCD_1	1805330	1806535	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
EEEDKGCD_1	586445	588370	tet(Q)	ARO:3000191	tet(Q)	94.07	97.56	Z21523.1
EEEDKGCD_1	583408	584544	tet(X)	ARO:3000205	tet(X)	99.74	97.42	M37699.1
EEEDKGCD_1	582383	583195	ErmF	ARO:3000498	ErmF	99.62	101.50	M17124.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
EEEDKGCD_1	582395	583195	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	M17808
EEEDKGCD_1	583408	584574	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.91	100.00	M37699
EEEDKGCD_1	1828235	1829137	cepA	Unknown Beta-lactam	99.67	100.00	L13472
EEEDKGCD_1	3177131	3179056	tet(Q)	Doxycycline, Tetracycline, Minocycline	97.66	100.00	L33696

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
EEEDKGCD_1	1828235	1829134	cepA	CepA family extended-spectrum class A beta-lactamase	EXACTX	100.00	100.00	WP_005795628.1
EEEDKGCD_1	582395	583192	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
EEEDKGCD_1	1804796	1805305	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
EEEDKGCD_1	583411	584574	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	BLASTX	99.74	100.00	WP_008651082.1
EEEDKGCD_1	1805333	1806535	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
EEEDKGCD_1	3177134	3179056	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.82	100.00	WP_002560998.1
EEEDKGCD_1	586448	588370	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	95.79	100.00	WP_063856407.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
EEEDKGCD_1	3475392	3476585	bft-2	BFT B. fragilis toxin, subtype 2	Exotoxin	100.00	100.00	BAA77277
EEEDKGCD_1	3475392	3476585	bft-3	BFT B. fragilis toxin, subtype 3	Exotoxin	96.90	100.00	BAA77275
EEEDKGCD_1	3475392	3476585	bft-1	BFT B. fragilis toxin, subtype 1	Exotoxin	94.89	100.00	BAA77276

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EEEDKGCD_1	0.0	1.0	0.0
EEEDKGCD_2	0.0	1.0	0.0
EEEDKGCD_3	0.0	1.0	0.0
EEEDKGCD_4	0.0	1.0	0.0
EEEDKGCD_5	0.0	1.0	0.0
EEEDKGCD_6	1.0	0.0	1.0
EEEDKGCD_7	1.0	0.0	1.0
EEEDKGCD_8	1.0	0.0	1.0
EEEDKGCD_9	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
EEEDKGCD_1	312855	320568	False	Siphoviridae	VOG4556,
EEEDKGCD_2	550403	562394	False	Unknown	VOG4572,

Phigaro v2.4.0 (Database: Jan. 2024 release)