Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 0 | 1 | 10.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GMMBOGCL_7 | 265152 | 266117 | CfxA2 | ARO:3003002 | CfxA2 | 99.69 | 100.00 | AF118110.1 |
| GMMBOGCL_8 | 66140 | 68065 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| GMMBOGCL_14 | 4486 | 5322 | APH(6)-Id | ARO:3002660 | APH(6)-Id | 99.64 | 100.00 | AF024602.1 |
| GMMBOGCL_14 | 3683 | 4486 | APH(3'')-Ib | ARO:3002639 | APH(3'')-Ib | 99.63 | 100.00 | AF313472.2 |
| GMMBOGCL_15 | 1889 | 2683 | APH(3')-IIa | ARO:3002644 | APH(3')-IIa | 100.00 | 100.00 | V00618.1 |
| GMMBOGCL_14 | 1604 | 2404 | ErmF | ARO:3000498 | ErmF | 99.62 | 100.00 | M17124.1 |
| GMMBOGCL_15 | 1496 | 1858 | BLMT | ARO:3005036 | BLMT | 100.00 | 95.24 | AB255435.1 |
| GMMBOGCL_16 | 503 | 1240 | ErmB | ARO:3000375 | ErmB | 98.37 | 98.79 | AF242872.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GMMBOGCL_6 | 158450 | 159316 | ant(6)-Ia | Streptomycin | 100.00 | 100.00 | KF864551 |
| GMMBOGCL_14 | 3683 | 4486 | aph(3'')-Ib | Streptomycin | 100.00 | 100.00 | AF321551 |
| GMMBOGCL_15 | 1889 | 2683 | aph(3')-IIa | Kanamycin, Neomycin, Kanamycin, Neomycin, Paromomycin, Ribostamycin, Butiromycin, Gentamicin | 100.00 | 100.00 | V00618 |
| GMMBOGCL_14 | 4486 | 5322 | aph(6)-Id | Streptomycin | 100.00 | 100.00 | M28829 |
| GMMBOGCL_14 | 1604 | 2404 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | M17808 |
| GMMBOGCL_14 | 2617 | 3461 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.88 | 72.41 | M37699 |
| GMMBOGCL_16 | 503 | 1240 | erm(B) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.86 | 100.00 | U18931 |
| GMMBOGCL_7 | 265152 | 266117 | cfxA3 | Ampicillin | 99.79 | 100.00 | AF472622 |
| GMMBOGCL_7 | 265152 | 266117 | cfxA4 | Unknown Beta-lactam | 99.79 | 100.00 | AY769933 |
| GMMBOGCL_7 | 265152 | 266117 | cfxA5 | Unknown Beta-lactam | 99.79 | 100.00 | AY769934 |
| GMMBOGCL_7 | 265152 | 266117 | cfxA | Cefoxitin, Ampicillin | 99.79 | 100.00 | U38243 |
| GMMBOGCL_8 | 66140 | 68065 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.79 | 100.00 | L33696 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GMMBOGCL_6 | 158453 | 159316 | aadE | aminoglycoside 6-adenylyltransferase AadE | EXACTX | 100.00 | 100.00 | WP_001255868.1 |
| GMMBOGCL_14 | 3683 | 4483 | aph(3'')-Ib | aminoglycoside O-phosphotransferase APH(3'')-Ib | EXACTX | 100.00 | 100.00 | WP_001082319.1 |
| GMMBOGCL_15 | 1892 | 2683 | aph(3')-IIa | aminoglycoside O-phosphotransferase APH(3')-IIa | EXACTX | 100.00 | 100.00 | WP_000572405.1 |
| GMMBOGCL_14 | 4486 | 5319 | aph(6)-Id | aminoglycoside O-phosphotransferase APH(6)-Id | EXACTX | 100.00 | 100.00 | WP_000480968.1 |
| GMMBOGCL_14 | 1604 | 2401 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | EXACTX | 100.00 | 100.00 | WP_002682030.1 |
| GMMBOGCL_15 | 1565 | 1858 | ble | bleomycin binding protein BLMT | PARTIALX | 100.00 | 77.78 | WP_000131935.1 |
| GMMBOGCL_14 | 2620 | 3459 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | PARTIALX | 100.00 | 72.16 | WP_008651082.1 |
| GMMBOGCL_7 | 265155 | 266117 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 99.69 | 100.00 | WP_004339683.1 |
| GMMBOGCL_16 | 506 | 1240 | erm(B) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | BLASTX | 99.59 | 100.00 | WP_002292226.1 |
| GMMBOGCL_8 | 66140 | 68062 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GMMBOGCL_7 | 133311 | 134891 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 80.64 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GMMBOGCL_1 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_2 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_3 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_4 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_5 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_6 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_7 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_8 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_9 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_10 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_11 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_12 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_13 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_14 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_15 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_16 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_17 | 0.0 | 1.0 | 0.0 |
| GMMBOGCL_18 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_19 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_20 | 1.0 | 0.0 | 1.0 |
| GMMBOGCL_21 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GMMBOGCL_1 | 303122 | 311307 | False | Unknown | VOG4573, |
| GMMBOGCL_2 | 191526 | 200315 | False | Siphoviridae | VOG4751, |
Phigaro v2.4.0 (Database: Jan. 2024 release)